New insights into the biophysical behavior of an old molecule: eperimental and theoretical studies of the interaction between 1,10-Phenanthroline and model phospholipid membranes]]> Alvarez, Natalia ]]> Freddi, Priscilla ]]> Castellani, Stephanie ]]> Veiga, Nicolás ]]> Facchin, Gianella ]]> Costa Filho, Antonio J. ]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> Adding sunflower or soybean oil to goat’s pasturebased diet improves the lipid profile without changing the sensory characteristics of milk]]> Dauber, Cecilia]]> Carreras, Tatiana]]> Casarotto Daniel, Gabriela]]> Cabrera, Fabiana]]> Liscano, Adriana]]> Vicente, Gabriela]]> Britos, Alejandro]]> Carro, Silvana]]> Cajarville, Cecilia]]> Gámbaro, Adriana]]> Vieitez, Ignacio]]>

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]]> Structure-based boisosterism design, synthesis, biological evaluation and in silico studies of benzamide analogs as potential anthelmintics]]> NEMATODA]]> Vairoletti , Franco]]> Paulino, Margot]]> Mahler, Graciela]]> Salinas, Gustavo]]> Saiz, Cecilia]]> Información sobre Derechos de Autor

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]]> A peculiar cell cycle arrest at g2/m stage during the stationary phase of growth in the wine yeast Hanseniaspora vineae.]]> Schwarz, Luisa Vivian]]> Valera, María José]]> Longaray Delamare, Ana Paula]]> Carrau, Francisco]]> Echeverrigaray, Sergio]]> Información sobre Derechos de Autor

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]]> Social representations of cooking and homemade meals]]> that the social representations of homemade meals were largely consensual among participants and mainly related to pleasure, health, and wellbeing. However, the social representations of cooking did not have a clear structure, which raised diverse associations related to homemade foods, positive emotions but also to lack of convenience in terms of time and effort. Results suggest public campaigns aimed at encouraging people to cook from scratch should position cooking as enjoyable task and diminish perception of lack of time and effort association.]]> Gugliucci, Vanessa]]> Vitola, Agustina]]> Girona, Alejandra]]> Santín, Viviana]]> Vidal, Leticia]]> Giménez, Ana]]> Curutchet, María Rosa]]> Ares, Gastón]]>

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]]> The impact of nutritional warnings on the mental associations raised by advertisements featuring ultra-processed food products]]> control advertisements or advertisements featuring black octagonal nutritional warnings. Right after watching the advertisements, they were asked to complete a word association task. Responses were grouped into 20 categories following inductive coding. A generalized linear model was used to assess the effect of experimental condition on the likelihood of providing responses within each of the identified categories. Participants exposed to the advertisements with (vs. without) warnings were more likely to provide responses related to NCDs or nutritional warnings. Meanwhile, no significant differences were found in the frequency of mention of categories related to sensory, hedonic, emotional, or social aspects linked to consuming the products between participants
exposed to advertisements with and without warnings. These results suggest that nutritional warnings may be a promising strategy to make consumers aware of the nutritional characteristics of ultra-processed products without affecting the ability of advertisements to convey their intended message.]]> Pérez, Lucía]]> Antúnez, Lucía]]> Otterbring, Tobias]]> Ares, Gastón]]>

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]]> Multiresidue analysis of basic, neutral, and acidic pesticides in biobeds’ biomixture]]> Contain between 1 and 3 bullet points highlighting the customization rather than the steps of the procedure. An analytical methodology was adjusted and validated for the analysis of 15 selected pesticides currently employed in extensive agriculture. The main application of this methodology is studying the pesticides degradation behavior using biobeds as a friendly environmental tool for the treatment of wastewaters generated in fields. The scope of the method was selected based on the most used pesticides in soybean crops in Uruguay. The novelty of this work is the inclusion of neutral and acidic herbicides such as 2,4-D, clethodim, dicamba, together with fungicides and insecticides which are usually included in Multi Residue Methods. An acetonitrile extraction methodology without a clean-up step yielded acceptable results for all the analytes. The instrumental analysis was performed using HPLC-MS/MS. The selected methodology was validated according to the SANTE guidelines. The recoveries were between 65 and 130% with RSD < 20%. The instrumental LOQs were fixed at 1 µg/L for all the compounds except for clethodim, and the method LOQs were 1 mg/kg in biomixture dry basis. These LOQs values are acceptable for biodegradation studies in biobeds.

A multiresidue methodology was:

• Validated for 15 pesticides in biomixture.

• Acidic herbicides were included in the scope.

• The method was employed for the environmental monitoring of pesticide degradation in biobeds.

]]> Rezende, Sofía]]> Besil, Natalia]]> Archondo, Lucas]]> Heinzen, Horacio]]> Cesio, María Verónica]]>

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]]> Hybrid Organic-Inorganic materials and Interfaces with mixed Ionic-Electronic transport properties: advances in experimental and theoretical approaches]]> Romero, Mariano]]> Mombrú, Dominique]]> Pignanelli, Fernando]]> Faccio, Ricardo]]> Mombrú, Álvaro W.]]> Información sobre Derechos de Autor

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]]> Structure elucidation of Triterpenoid Saponins found in an immunoadjuvant preparation of Quillaja brasiliensis using mass spectrometry and 1H and 13C NMR spectroscopy]]> Wallace, Federico]]> Fontana, Carolina]]> Ferreira, Fernando]]> Olivaro, Cristina]]> Información sobre Derechos de Autor

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]]> Diversity and effect of increasing temperature on the activity of methanotrophs in sediments of fildes Peninsula Freshwater Lakes, King George Island, Antarctica]]> temperature on the CH4 oxidation potential in sediments of freshwater lakes in polar regions of the southern hemisphere.]]> Roldán, Diego M.]]> Carrizo, Daniel]]> Sánchez García, Laura]]> Menes, Rodolfo Javier]]> Información sobre Derechos de Autor

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]]> Sensitivity of Haemonchus contortus to anthelmintics using different in vitro screening assays: a comparative study]]> Background: Helminthiasis and resistance to commercial anthelmintic compounds are major causes of economic
losses for livestock producers, resulting in an urgent need for new drugs and reliable in vitro screening tests capable
of detecting potentially active products. Considering this, a series of novel benzimidazole derivatives (5-methylbenzimidazole 1,2-disubstituted, 5-carboxybenzimidazole, 5-methylbenzimidazole 2-one) was screened on exsheathed L3 (xL3) and on the adult stage of Haemonchus contortus (Kirby anthelmintic-susceptible McMaster isolate).
Methods: This work presents the set-up of an automated motility assay on the xL3 stage of H. contortus using an
infrared tracking device (WMicrotracker One) together with a larval development test (xL3 to L4) and a motility assay
on the adult stage of H. contortus. A comparative study of the sensitivity of these in vitro assays using commercial
anthelmintics with different mechanisms of action was carried out, also evaluating anthelmintic activity of a series of
novel benzimidazole derivatives.
Results: The automated xL3 assay had the great advantage of being able to analyze many compounds simultaneously,
but it showed the limitation of having lower sensitivity, requiring higher concentrations of the commercial
anthelmintics tested compared to those needed for the adult motility or development assays. Although none of the
novel 1,2,5-tri-substituted benzimidazole derivatives could significantly decrease the motility of xL3s, one of them (1e)
significantly affected the development of xL3s to L4, and five new compounds (1b, 1d, 1e, 2a and 2c) reduced the
motility of H. contortus adult stage.
Conclusions: The analysis of the results strongly suggests that the in vitro xL3 to L4 development test, particularly
for the L4 stage, could be closer to the pharmacological sensitivity of the adult stage of H. contortus (target of interest)
for commercial anthelmintic selected, with different mechanisms of action, and for the series of benzimidazole
derivatives assayed. Therefore, an automated motility assay on L4 using the infrared tracking device is being set up.
Further studies will be conducted to evaluate the in vivo anthelmintic activity of the most active novel benzimidazole
derivatives.

]]> Munguía, Beatriz]]> Saldaña, Jenny]]> Nieves, Magdalena]]> Melian, María Elisa]]> Ferrer, Manuela]]> Teixeira, Ramiro]]> Porcal, Williams]]> Manta, Eduardo]]> Domínguez, Laura]]> BioMed Central]]> Información sobre Derechos de Autor

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]]> Phylogeny and identification of pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay]]> Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens.]]> De Armas, Stefanie]]> Galván, Guillermo A.]]> Lapaz, María I.]]> González Barrios, Pablo]]> Vicente, Esteban]]> Pianzzola, María J.]]> Siri, María I.]]> Información sobre Derechos de Autor

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]]> Secretion and detection of defensive compounds by the red flour beetle tribolium castaneum interacting with the insect pathogenic fungus beauveria bassiana]]> Información sobre Derechos de Autor

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]]> Can children use the A-not a test?]]> results. A total of 126 children were involved in the study. They participated in three sessions, each composed of a familiarization task with a visual stimuli and sample tasting with
one of three dairy products (vanilla milk desserts, chocolate-flavored milk, and vanillaflavored yogurt). Half of the children evaluated the samples in terms of overall differences and the other half in terms of differences in liking. Results from the familiarization step showed that children correctly identified the visual reference in the A-not-A test regardless of how the question was framed, suggesting that they were able to understand the methodology. In the case of tasting samples, children were significantly more likely to correctly identify the reference in two of the three studied dairy products, when the question was based on liking, as compared to the framing based on overall differences.]]> Velázquez, Ana Laura]]> Vidal, Leticia]]> Varela, Paula]]> Ares, Gastón]]> Información sobre Derechos de Autor

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]]> Season and No-Till Rice Crop Intensification Affect Soil Microbial Populations Involved in CH4 and N2O Emissions]]> Fernández Scavino, Ana]]> Oreggioni, Daniela]]> Martínez Pereyra, Andrea]]> Tarlera, Silvana]]> Terra, José A.]]> Irisarri, María del Pilar]]>

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]]> Methanogenic community linked to organic acids fermentation from root exudates are affected by rice intensification in rotational soil systems]]> Pereira Mora, Luciana]]> Terra, José A.]]> Fernández Scavino, Ana]]>

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]]> Synthesis of Pyrethroids and Jasmonoids through Palladium-Catalyzed Cross-Coupling Reactions]]> Parpal, Florencia]]> Paullier, Ana Paula]]> Pandolfi, Enrique]]> Heguaburu, Viviana]]>

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]]> Synthetic Study of 5-Hydroxymethylfurfural in Groebke-Blackburn-Bienaymé Reaction]]> de la Sovera, Victoria]]> López, Gloria V.]]> Porcal, Williams]]>

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]]> Metabolic engineered E. coli for the production of (R)-1,2-propanediol from biodiesel derived glycerol]]> Rodríguez Giordano, Sonia]]>

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ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

]]> Detection of organochlorine pesticides in water and biological matrix]]> Umpiérrez, Eleuterio F.]]> Díaz, Daniela Ariadna]]>

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]]> Consumer perspectives on the inclusion of nutritional warnings in retail food establishments ]]> El presente trabajo tuvo como objetivo evaluar la percepción de los consumidores uruguayos en relación a la implementación de advertencias nutricionales en cuatro puntos de venta de comida y generar ideas sobre cómo podría implementarse esta medida. Se realizó un estudio online con una muestra no probabilística de 547 participantes, reclutados utilizando publicidad en redes sociales. Los participantes indicaron su grado de acuerdo con la implementación de advertencias nutricionales en panaderías, rotiserías, lugares de venta de comida rápida y sitios de pedidos online, y proporcionaron ideas sobre cómo incluir esta inforamción en cada punto de venta. Los resultados sugieren que la población tiene una perecpeción positiva sobre la inclusión de advertencias nutricionales en puntos de venta de comida. Los participantes destacaron la importancia de incluir esta información en un lugar visible al momento de elegir los productos, de forma que pueda ser tenida en cuenta al tomar las decisiones. En particular, las vitrinas y la cartelería/pizarras fueron identificados como lugaresestatégicos para la inclusión de advertencias nutricionales en panaderías/rotiserías y puntos de venta de comida rápida, respectivamente. Los resultados proporcionan valiosa información para extender la inclusión de advertencias nutricionales a puntos de venta de comida.]]> Antúnez, Lucía]]> Curutchet, María Rosa]]> Moratorio, Ximena]]> Giménez, Ana]]> Ares, Gastón]]> Información sobre Derechos de Autor

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]]> Straightforward sample preparation method for the analysis of pesticide residues in ginger (Zingiber officinale Rosc.) using gas chromatography-tandem mass spectrometry]]> Zingiber officinale Rosc., “ginger”, is widely cropped for its use as a spice and raw material for phytotherapeuticals. During cropping or post-harvest, different phytosanitary products are used, whose residues can be found in the final product, lowering the, quality and safety of the raw material. To detect those residues, a multi-contaminant methodology for GC–MS/MS analysis was developed and validated. For the selection of most suitable methodology, the performance of three methods in the determination of 39 analytes were compared: QuEChERS, QuEChERS AOAC 2007.01, and a modification of the AcOEt method, based on the inclusion of Al₂(SO₄)₃ in the extraction step to form stable and ethyl acetate insoluble Aluminum- (gingerols and/or polyphenols) complexes, lowering the co-extractives load injected in the instrument. Compared to the Acetonitrile based methods, the AcOEt method showed the best performance as 95% of the targeted analytes yielded recoveries between 70 and 120 and 80% of them between 90 and 110%). The method was validated for 57 contaminant residues by GC–MS/MS. The figures of merit (media of recoveries 96% and RSD 16%, back calculated concentration < 20%, matrix effects > 100%, linearity 5–300 μkg-¹) of the method accomplished the SANTE guidelines and the LOQs for all compounds (10-25μkg^-1) were below the ginger MRLs defined by food and medicinal herbs regulations, to ensure its safe consumption.

]]> Gérez García, Natalia]]> Zinola, Gianna]]> Macías, Gonzalo]]> Cesio, María Verónica]]> Heinzen, Horacio]]> Información sobre Derechos de Autor

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]]> Design and construction of a low-cost, in-situ analyzer for nutrients in surface waters, based on open-source hardware and software]]> This paper explains the design and construction of an automated analyzer for the in-situ determination of nutrients in surface waters. The analyzer is based on a pulsed flow analysis system employing solenoid micropumps and uses photometric detection based on LEDs and photodiodes. The analytes it determines are orthophosphate, nitrite and nitrate. The analyzer was programmed to perform a complete analysis cycle every 6 h. The end user receives the information from the analyzer via text messages (SMS), a service available even in rural and less developed areas, and requiring simple (non-smart) cell phones. The linear range was up to 25 mg L^-1P for phosphate, up to 1600 μg L^-1N-NO₂for nitrite, and up to 2500 μg L^-1N-NO₃for nitrite plus nitrate. The detection limits for each analyte were 0.28 mg L^-1P for phosphate, 19 μg L^-1N-NO₂for nitrite and 26 μg L^-1N-NO₃for nitrite plus nitrate. The analyzer can perform analysis cycles every 6 h with an autonomy of one week. It is suitable as a versatile analytical platform for environmental research.

]]> González, Pablo]]> Pérez, Nicolás]]> Knochen, Moisés]]>

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]]> Flavonoids and limonoids profiles variation in leaves from mandarin cultivars and its relationship with alternate bearing]]> Alternate bearing in citrus trees has been extensively studied as a key feature for citrus growers. Although the genetic and the biochemical process occurring during alternate bearing has been studied extensively, there is a lack of information identifying the presence of metabolic indicators during “on” and “off” years. In citrus plants, leaves play a central role in the metabolic pathway triggering the flflowering induction process. To investigate the changes during this transition, a liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis of the leaf profifiles of 20 compounds (17 polyphenols, two limonoids, and one furanocoumarin), in bearing and non-bearing branches arising from four different mandarin genotypes, was performed. The same metabolites were found in all the genotypes at both stages: both limonoids and 11 polyphenols.

Using these compounds, the chemotaxonomic differentiation between cultivars was assessed. The levels of flflavanones and limonoids showed differences in both bearing stages and the transition from vegetative to flflowering could be shown by the activation of the polyphenol biosynthetic pathway, from precursors like naringenin to metabolic end-points such as narirutin and polymethoxyflflavones.

Narirutin levels showed signifificant differences between both stages, suggesting it as a possible marker of the physiological status of the branch.

]]> Rodríguez Ceraolo, Cecilia]]> Vázquez, Valeria]]> Migues, Ignacio]]> Cesio, María Verónica]]> Rivas, Fernando]]> Heinzen, Horacio]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Electronic structure properties of boron–doped and carbon–boron–codoped TiO2(B) for photocatalytic applications]]> Heffner, Herman]]> Faccio, Ricardo]]> López Corral, Ignacio]]> Información sobre Derechos de Autor

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