Genetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay]]> Clavijo, Felipe

]]> Curland, Rebecca D.

]]> Croce, Valentina

]]> Lapaz, María Inés

]]> Dill-Macky, Ruth

]]> Pereyra, Silvia

]]> Siri, María Inés

]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derechode autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> Fernández, Gerónimo]]> Sbres, Mauricio]]> Lado Lindner, Joanna]]> Pérez Faggiani, Elena]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> “Ready-to-use” immunosensor for the detection of small molecules with fast readout]]> Immunoassays are commonly used methods for detection of small molecules that typically require numerous steps of the labeling between immune-recognition reagents and tracers, immobilization and recurrent washing, making them time consuming and difficult to adapt into point of care formats. Here we describe a “ready-to-use” homogeneous competitive immunosensor with an assay time of 10 min that is based exclusively on recombinant reagents. The signal is produced when the split fragments of the nano luciferase (Nluc) are brought together by the interaction of a heavy chain only variable domain (VHH) with a peptidomimetic of the target small molecule. A VHH to 2,4-dichlorophenoxyacetic acid (2,4-D) was used to isolated the peptidomimetic (NGFFEPWQVVYV)
from phage display libraries using six panning conditions. Then the peptidomimetic and VHH were fused with the larger (LgN) and smaller piece (SmN) of split fragments of Nluc, respectively. In order to optimize the signal and sensitivity of the immunosensor, we explored the effects of the spacer between the peptidomimetic and LgN, the copy number of peptidomimetics, and the spacer between SmN and VHH, generating 24 combinations that allowed to conclude on their respective roles. Eventually, the developed “ready-to-use” immunosensor performed excellent signal-to-noise ratio and sensitivity, and could be applied to the detection of 2,4-D in real samples. Meanwhile, the immunosensor totally realizes labeling-free, immobilization-free and washing-free, also can be
produced in a highly cost effective way.

]]> Ding, Yuan]]> Cui, Panpan]]> Chen, He]]> Li, Jiao]]> Huang, Lianrun]]> González Sapienza, Gualberto]]> Hammock, Bruce D.]]> Wang, Minghua]]> Hua, Xiude]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Characterization and incorporation of extracts from olive leaves obtained through maceration and supercritical extraction in Canola oil: oxidative stability evaluation ]]> Olive leaves (OL) are considered a potential source of bioactive compounds mainly due to its high content of phenolic compounds, widely known as natural antioxidants. The main objective of this study was to compare the performance of three OL extracts obtained by different extraction techniques in protecting canola oil against oxidative damage. The technologies evaluated were maceration with ethanol/water 75:25 (v/v), supercritical fluid extraction with CO2 (SC–CO2) and SC-CO2 with 10% ethanol as modifier (SC–CO2/EtOH). Each extract was analyzed as for total phenolic compounds (TPC), antioxidant activity (ABTS assay) and phenolic composition by reversed phase liquid chromatography-quadrupole-time of flight mass spectrometry. The oxidative stability of canola oil with or without the incorporation of 250 mg/kg of each extract was assessed during five weeks of
storage at 60 ◦C. Peroxide, K232, K270, and Rancimat values, besides tocopherols content were determined.
Macerated extract showed the highest TPC and antioxidant activity, but both SC-CO2 extracts were more effective in preserving tocopherols. In addition, SC-CO2 extracts delayed the oxidation progress as they lead to higher induction periods than control and macerated extracts, and a slower increase in peroxide values. Results obtained reinforce the use of supercritical fluid technology to obtain antioxidants compounds from natural sources.

]]> Dauber, Cecilia]]> Carreras, Tatiana]]> González, Laura]]> Gámbaro, Adriana]]> Valdés, Alberto]]> Ibáñez, Elena]]> Vieitez, Ignacio]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Immobilized peptide-N-glycosidase F onto magnetic nanoparticles: a biotechnological tool for protein]]> The elucidation of glycans biological function is essential to
understand their role in biological processes, both normal and
pathological. Immobilized glycoenzymes are excellent tools
for this purpose as they can selectively release glycans from
glycoproteins without altering their backbone. They can be
easily removed from the reaction mixture avoiding their
interference in subsequent experiments. Here, we describe the
immobilization of peptide-N-glycosidase F (PNGase F) onto
silica magnetic nanoparticles with immobilization yields of
86% and activity yields of 12%. Immobilized PNGase F showed
higher thermal stability than its soluble counterpart, and could
be reused for at least seven deglycosylation cycles. It was
efficient in the deglycosylation of several glycoproteins
(ribonuclease B, bovine fetuin, and ovalbumin) and a protein
lysate from the parasite Fasciola hepatica under native
conditions, with similar performance to that of the soluble
enzyme. Successful deglycosylation was evidenced by a
decrease in specific lectin recognition of the glycoproteins
(40%–80%). Moreover, deglycosylated F. hepatica lysate
allowed us to confirm the role of parasite N-glycans in the
inhibition of the lipopolysaccharide-induced maturation of
dendritic cells. Immobilized PNGase F probed to be a robust
biotechnological tool for deglycosylation of glycoproteins and
complex biological samples under native conditions.

]]> Bidondo, Lucía]]> Festari, Florencia]]> Freire, Teresa]]> Giacomini, Cecilia]]> Información sobre Derechos de Autor

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Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> How are the sensory properties perceived by consumers?: a case study with pressurized tropical mixed juice]]> min). Thirty fruit juice consumers participated in the characterization of mixed juices using rate-all-that-apply (RATA) questions. Samples were analyzed at zero, 14, and 28 days of storage at 4 ◦C. Data were analyzed by ANOVA, and Fisher’s test was used for post-hoc comparison of means and PCA. Results showed that 12 of the 22 RATA terms differed (p ≤ 0.05) regarding the mean perceived intensity, suggesting that consumers perceived
differences in the sensory characteristics of the juices. The first dimension of the PCA separated the pasteurized juices from the others. Pasteurized samples were characterized by yellow color, cooked aroma, cooked flavor, off aroma, off flavor, stale aroma, and stale flavor. On the other hand, the unprocessed (control) and pressurized (HPP1 and HPP2) juices were characterized by orange color, fruit aroma, fruit flavor, sweet aroma, fresh juice, and watery consistency. Results confirmed the suitability of the sensory characterization performed by consumers using RATA and the potential of HHP to obtain juices with sensory characteristics similar to the fresh product.]]> Araújo Martins, Inayara Beatriz]]> Souza, Carla Regina de]]> Alcantara, Marcela de]]> Rosenthal, Amauri]]> Ares, Gastón]]> Deliza, Rosires]]>

Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Age, time orientation and risk perception are major determinants of discretionary salt usage]]> The present work explored the relationship between discretionary salt usage and personal characteristics, using
boosted regression trees (BRT). Specifically, the focus was on how socio-demographic characteristics and personality traits linked to risk perception and time orientation impact on discretionary salt consumption patterns. For this purpose, an online cross-sectional survey with a convenience sample of 498 Uruguayan participants was carried out. Participants completed the consideration of future consequences (CFC) scale adapted for eating behaviour, a short survey about discretionary salt consumption patterns and indicated their degree of agreement
with statements measuring perceived risk of sodium consumption. Finally, socio-demographic data were
collected. BRT were applied to build predictive models that related discretionary salt usage to socio-demographic
characteristics, the two factors of the CFC-Food scale (consideration of the future and consideration of the immediate
consequences of eating behaviour), and the two factors of the perceived risk of sodium consumption scale (severity of perceived risks and risk compensation). Age, time orientation and perceived risk were the most relevant explanatory variables for discretionary salt usage. Older people had a lower likelihood of adding salt to food, either at home or when eating out. In addition, individuals who tend to be present rather than future
oriented, as well as those with low perception of risk severity and susceptibility were more likely to add salt to foods. Results from the present work suggest that communication campaigns to reduce discretionary salt intake should mainly focus on stressing the short-term health benefits of reducing sodium intake and raising perceived susceptibility.

]]> Antúnez, Lucía]]> Vidal, Leticia]]> Giménez, Ana]]> Curutchet, María Rosa]]> Ares, Gastón]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Co-creation of a healthy dairy product with and for children]]> Children’s involvement in new product development may contribute to ensure that healthy alternatives meet their wants and needs. Co-creation is a potential approach to bridge the world of the child and the grown-up product developer. In this context, the objective of the present work was to explore the potential of a cocreation approach with children to develop a healthy dairy product. A total of 52 school-aged children (54%
girls, 6–13 year old) recruited from an after-school club in Montevideo (Uruguay) participated in co-creation workshops. Two sessions were carried out. In the first session, children developed a dairy product using a set of ingredients and proposed strategies to promote their product. Children showed a high level of engagement during the activity and provided actionable ideas for product development. Three key factors drove the product formulation (familiarity, hedonics, and healthiness), whereas common communication strategies were proposed by the participants to promote their products. In the second session, children evaluated four products developed
by the researchers based on children’s ideas. The products showed high overall liking scores (5.9 to 7.9) despite the inclusion of novel and healthy ingredients and the lower sugar content compared to similar commercial products available in the Uruguayan marketplace. These results show the potential of involving children in the co-creation of healthy dairy products. Considering the lack of published studies regarding the use of co-creation approaches with children in the food domain, the present work provides useful insights for the implementation of
co-creation to develop innovative products with and for children.

]]> Velázquez, Ana Laura]]> Galler, Martina]]> Vidal, Leticia]]> Varela, Paula]]> Ares, Gastón]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> “I don’t throw away food, unless I see that it’s not fit for consumption”: an in-depth exploration of household food waste in Uruguay]]> Significant reductions in household food waste have been regarded as a key step towards achieving global sustainable development. Household food waste is a complex phenomenon determined by consumer behavior along the steps of the “food journey” that goes from purchasing to final disposal. Although avoiding food waste is socially desirable and raises positive attitudes, consumers do not frequently engage in avoidance behaviors. The objectives of the present work were: i) to explore the views of Uruguayan citizens on household food waste, and ii) to identify drivers of food waste among Uruguayan households. A total of 20 in-depth interviews based on a semi-structured guide were conducted by telephone. Participants were asked to recall and describe the last time
they discarded food, as well as to describe the most common food waste situations in their household, the most commonly used strategies to avoid food waste and how they could reduce it. The transcripts were analyzed using content analysis based on a deductive-inductive approach. Interviews revealed that most of the participants perceived food waste in their homes as null or low, whereas food waste in the country was regarded as high. When participants described food waste incidents, they perceived it as ’unavoidable’, suggesting that they tended
to find a rational explanation outside of their will to justify their behavior. Participants’ discourses enabled the identification of drivers related to behavioral factors, personal factors, product factors, and contextual factors. Results stress that most promising entry points for communication campaigns and intervention programs to reduce household food waste should focus on behavioral factors, planning throughout all the household stages of the food journey and the provision of knowledge and skills on food storage, handling, and preparation.

]]> Ferro, Camila]]> Ares, Gastón]]> Aschemann Witzel, Jessica]]> Curutchet, María Rosa]]> Giménez, Ana]]> Información sobre Derechos de Autor

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]]> Dauber, Cecilia]]> Carreras, Tatiana]]> Fernández Fernández, Adriana]]> Irigaray, Bruno]]> Alborés, Silvana]]> Gámbaro, Adriana]]> Ibáñez, Elena]]> Vieitez, Ignacio]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Measuring consumer attitudes using text highlighting: methodological considerations]]> Direct attitude questions occupy a central position in consumer research despite concerns over validity and reliability. Text highlighting is an alternative method where participants use highlighter functions to indicate content that they ’ike’ and ’dislike’ as they read a text. Jaeger et al. (2022) [Food Qual Prefer, 95, 104356] introduced this approach and the present research investigated it methodologically to further assess its potential for attitude measurement. There were two research questions (RQ) that asked whether: 1) highlighting responses
are independent of text characteristics (length, content); and 2) consumers’ attitudes are altered by the process of
completing the highlighting task. Data were collected in an online survey on vertical farming (VF) where 1466 UK consumers took part. For RQ1, results showed that word highlighting frequency was altered by textual context and decreased for longer texts. The sentiment scores for sentences were also altered, but the characteristics about VF that consumers responded positively and negatively to were minimally influenced by text length and content. For RQ2, results showed that completion of the text highlighting task did not influence attitudes to VF, meaning that the task of highlighting did not introduce response bias. Collectively, the findings of the present research further support that text highlighting has potential for attitude measurement.

]]> Jaeger, Sara R.]]> Ares, Gastón]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Comparison of variants of the valence × arousal circumplex-inspired emotion word questionnaire]]> Product-focused emotion research has been in ascendency for more than a decade, but methodological research into questionnaires, the most popular tool for measuring emotional associations is still needed. The present research was situated in this context and pertained to the valence × arousal circumplex-inspired emotion questionnaire (CEQ) which includes 12 pairs of emotion words. Variants of the CEQ that pertained to response options (single vs. multiple) and layout (circular vs. list) were compared in four consumer studies with a total of 1857 participants. In Studies 1 to 3, the CEQ variant that featured a circular layout and single response format
(C&S) was compared to a list variant with multiple response format (L&M) (i.e., a check-all-that-apply question).
Selection of CEQ emotion word pairs and degree of sample discrimination was contingent on CEQ variant, meaning that conclusions about sample similarities and differences depended on the CEQ variant that was used.
This was also the result in Study 4 where two circular CEQ variants were compared, with single and multiple response formats, respectively. A further important finding was that stimuli format – tasted samples in Studies 1–2 and written stimuli in Studies 3–4 – as well as degree of sample differences influenced the extent to which the results from the CEQ variants differed. Our recommendations at this stage are to be aware that conclusions about sample profiles and discrimination is CEQ variant dependent and avoid directly comparing results from studies that use different CEQ variants. Implementing the CEQ as a multi-response variant is supported since it improves
sample discrimination relative to single response data, particularly for pairs of emotion words that primarily
relate to the dimension of arousal.

]]> Jaeger, Sara R.]]> Ryan, Grace S.]]> Jin, David]]> Ares, Gastón]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Text highlighting for consumer insights: influence of text length and difficulty]]> Text highlighting is a novel method for measuring consumer attitudes where participants read information about a focal topic and use highlighting functions to select aspects of the text that they like and dislike. The present research contributed methodological knowledge about text highlighting by investigating how responses are influenced by two aspects of the texts — length and degree of reading difficulty. A case study pertaining to biodynamic agriculture was used to assess the research questions and empirical data were collected from 3718 consumers across four countries (United Kingdom, Australia, Germany, and Singapore). Results showed that both text length and reading difficulty influenced responses — overall frequency of highlighting, frequency of ‘like’ highlighting, frequency of ‘dislike’ highlighting, and sentiment scores — leading to recommendations about future implementations of the methodology. Specifically, a single highlighting task on a longer text is less recommended than consecutive highlighting tasks on shorter texts. Implementation of the latter increases highlighting frequency and is expected to be associated with greater participant task engagement. Text length also influenced sentiment scores but did so in a manner that was topic and content specific. Regarding text difficulty, significant differences were established for all types of highlighting responses, although the differences were smaller than found for text length. The recommendation is to use simple and familiar language that is suited to the groups of participants taking part in the study. A general recommendation is to interpret findings in the context of the presented information.

]]> Jaeger, Sara R.]]> Chheang, Sok L.]]> Ares, Gastón]]> Información sobre Derechos de Autor

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]]> The “Other Vassoura Oil” and volatile fractions from Baccharis uncinella DC. (Asteraceae) as potential sources for flavor and fragrance Industry.]]> Baccharis L. (Asteraceae) contains more than 440 species of
exclusively American distribution, from USA to Argentina,
concentrated mostly in the highland areas of southern Brazil and throughout the Andes. Besides its ethnopharmacological/ medicinal importance, Baccharis volatile fractions (VFs) are important sources of bioactive compounds for flavor and fragrance industry, mainly the species B. dracunculifolia DC. and in a lesser extention B. trimera (Less.), whose essential oils (vassoura and carqueja oil, respectively) are in commerce. However, research and valorization of other Baccharis species as aroma sources remains scarce to date. In this chapter we discuss the relevance of B. uncinella DC., the species more
closely related genetically to B. dracunculifolia. Focusing our study on the different techniques and protocols applied to obtain B. uncinella VFs, namely steam distillation (SD, at both, laboratory- and pilot-scales to obtain the “other vassoura essential oil”), simultaneous distillation- extraction (SDE), and supercritical fluid extraction (SFE). The chemical identification and quantitation of VFs was carried out by conventional gas chromatography (GC-FID) and gas chromatography-mass spectrometry (quadrupole analyzer) (GC-qMS). A close look at the data indicated that the chemical composition of B. uncinella VFs largely depended on the extraction technique, thus significantly affecting their aromatic potential. Only SDE allowed the recovery of lighter volatile compounds as C6 components (i.e., “green leaf volatiles”), pilot-scale SD concentrated more hydrocarbons and fewer oxygenated compounds compared to laboratory-scale SD, and SFE recovered several oxygenated monoterpenes and polyquinane sesquitepenes (tentative identification) not obtained by the other techniques. The main components found in the VFs were α-pinene, β-pinene, limonene, (E)-caryophyllene, germacrene D, bicyclogermacrene, spathulenol, and caryophyllene oxide; most of them olfactorily relevant compounds according to previous studies. Therefore, the importance of B. uncinella, its VFs and the “other vassoura oil” as potential sources of aromatic compounds in the flavor and fragrance industry is highlighted.

]]> Minteguiaga, Manuel]]> Catalán, César A. N.]]> Cassel, Eduardo]]> Dellacassa, Eduardo]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Comparative study of antioxidant and pro-oxidant properties of homoleptic and heteroleptic copper complexes with amino acids, dipeptides and 1,10-Phenanthroline: the quest for antitumor compounds.]]> In a search for new antitumoral agents, a series of homoleptic copper(II) complexes with amino acids and dipeptides, as well as heteroleptic complexes containing both dipeptides and
1,10-phenanthroline, were studied. Furthermore, a single-crystal structure containing alanyl-leucinato ([Cu3(AlaLeu)3 (H2O)3(CO3)]PF6H2O), which is the first homotrinuclear carbonato-bridged copper(II) complex with a dipeptide moiety, is presented. To assess possible antitumor action mechanisms,
we focused on the comparative analysis of pro- and antioxidant behaviors. Pro-oxidant activity, in which the reactive oxygen species (ROS) formed by the reaction of the complexes with H2O2 produce oxidative damage to 2-deoxy-D-ribose, was evaluated using the TBARS method. Additionally, the antioxidant action was quantified through the superoxide dismutase (SOD)-like activity, using a protocol based on the inhibitory effect of SOD on the reduction of nitrobluetetrazolium (NBT) by the superoxide anion generated by the xanthine/ xanthine oxidase system. Our findings show that Cu–amino acid complexes are strong ROS producers and moderate SOD mimics. Conversely, Cu–dipeptide–phen complexes are good SOD mimics but poor ROS producers. The activity of Cu–dipeptide complexes was strongly dependent on the dipeptide. A DFT computational analysis revealed that complexes with high SOD-like activity tend to display a large dipole moment and condensed-to-copper charge, softness and LUMO contribution. Moreover, good ROS producers have higher global hardness and copper electrophilicity, lower copper softness and flexible and freely accessible coordination polyhedra.

]]> Veiga, Nicolás]]> Alvarez, Natalia]]> Castellano, Eduardo E.]]> Ellena, Javier]]> Facchin, Gianella.]]> Torre, María H.]]> Información sobre Derechos de Autor

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]]> Structural insights in galectin-1-glycan recognition : relevance of the glycosidic linkage and the N-acetylation pattern of sugar moieties]]> Galectins, soluble lectins widely expressed intra- and extracellularly in different cell types, play major roles in
deciphering the cellular glycocode. Galectin-1 (Gal-1), a prototype member of this family, presents a carbohydrate
recognition domain (CRD) with specific affinity for β-galactosides such as N-acetyllactosamine (β-D-Galp-(1
→ 4)-D-GlcpNAc), and mediate numerous physiological and pathological processes.
In this work, Gal-1 binding affinity for β-(1 → 6) galactosides, including β-D-Galp-(1 → 6)-β-D-GlcpNAc-(1 → 4)-
D-GlcpNAc was evaluated, and their performance was compared to that of β-(1 → 4) and β-(1 → 3) galactosides.
To this end, the trisaccharide β-D-Galp-(1 → 6)-β-D-GlcpNAc-(1 → 4)-D-GlcpNAc was enzymatically synthesized, purified and structurally characterized.
To evaluate the affinity of Gal-1 for the galactosides, competitive solid phase assays (SPA) and isothermal titration calorimetry (ITC) studies were carried out. The experimental dissociation constants and binding energies obtained were compared to those calculated by molecular docking. These analyses evidenced the critical role of the glycosidic linkage between the terminal galactopyranoside residue and the adjacent monosaccharide, as galactosides bearing β-(1 → 6) glycosidic linkages showed dissociation constants six- and seven-fold higher than those involving β-(1 → 4) and β-(1 → 3) linkages, respectively. Moreover, docking experiments revealed the presence of hydrogen bond interactions between the N-acetyl group of the glucosaminopyranose moiety of the evaluated galactosides and specific amino acid residues of Gal 1, relevant for galectin-glycan affinity. Noticeably, the binding free energies (ΔGcbainlcd) derived from the molecular docking were in good agreement with experimental values determined by ITC measurements (ΔGebxinpd), evidencing a good correlation between theoretical and experimental approaches, which validates the in silico simulations and constitutes an important tool for the rational design of future optimized ligands.

]]> Porciúncula González, Cecilia]]> Cagnoni , Alejandro J.]]> Fontana, Carolina]]> Mariño, Karina V.]]> Saenz Méndez, Patricia]]> Giacomini, Cecilia]]> Irazoqui, Gabriela]]> Información sobre Derechos de Autor

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]]> Synthesis and characterization of high temperature properties of YBa2Cu3O6+δ superconductor as potential cathode for intermediate temperature solid oxide fuel cells]]> YBa2Cu3O6+δ (YBC) oxygen deficient perovskite was synthesized by an auto-combustion method and was studied as potential cathode for Intermediate Temperature Solid Oxide Fuel Cell (IT-SOFC). Synchrotron X-ray thermodiffraction in air shows a phase transition from orthorhombic Pmmm to tetragonal P4/mmm space groups at ~ 425°C. The chemical compatibility with Ce0.9Gd0.1O1.95 (GDC) electrolyte was investigated in air where certain reactivity was observed above 800 °C. However, the main phase is Ba(Ce1-xYx)O3, a good ionic conductor. The catalytic performance in air was obtained by electrochemical impedance spectroscopy (EIS) measurements on YBC/GDC/YBC symmetrical cells. The area specific resistance (ASR) values change from 13.66 to 0.14 Ω cm2 between 500 and 800 °C, with activation energy (Ea) of 0.41 eV. The results suggest potential applications of YBC as IT-SOFC cathode.]]> Grassi, Joaquín]]> Macías, Mario A.]]> Basbus, Juan F.]]> Castiglioni, Jorge]]> Gauthier, Gilles H.]]> Serquis, Adriana C.]]> Suescun, Leopoldo]]> Zeal Press]]> Información sobre Derechos de Autor

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]]> The impact of sensory attributes of mandarins on consumer perception and preferences]]> Although the sensory characterization of mandarins has usually been performed by trained assessors, in recent years, the methodologies that depict sensory descriptions from the perception of consumers, such as the checkall- that-apply (CATA) questionnaires have become very popular. In this work, an exploratory study was first carried out using a focus group (FG) technique to determine attributes and aspects that influence the decision to purchase mandarins. Secondly, the consumer panel recruited 100 regular mandarin consumers that were randomly divided into two groups. Group “A” received segments of 15 mandarins. Group “B”, instead of receiving the same mandarins in segments, they received them as a whole fruit having to peel them by themselves. Both groups evaluated the total acceptability with a 9-point hedonic scale and answered a CATA questionnaire composed of 38 terms for Group “A” and 52 terms for Group “B”. Although the consumers in the FG described an "ideal" mandarin as easy-to-peel, no significant difference (p > 0.05) was found in the acceptability
between groups “A” and “B”. Characteristics such as sweet, very sweet, fresh smell, sweet smell, bright, fruity taste, fresh flavor, typical flavor, balanced taste and nice flavor were strongly and positively correlated with overall consumers’ acceptance.

]]> Gámbaro, Adriana]]> Roascio, Antonella]]> Hodos, Natalia]]> Migues, Ignacio]]> Lado Lindner, Joanna]]> Heinzen, Horacio]]> Rivas, Carlos Fernando]]> Información sobre Derechos de Autor

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]]> Variability levels of selected amino acids among mandarins produced in Uruguay]]> Nutraceutical properties of mandarins are of great interest to promote their consumption. The occurrence of free amino acids in foods is relevant to assess the nutritional value of it. To learn more about the amino acids’ occurrence and variability between species, a targeted metabolomics study in ‘Ellendale’, ‘Willowleaf’ and ‘Page’ varieties was performed through ion exchange liquid chromatography coupled to tandem mass spectrometry. The studied amino acids were asparagine, glutamic acid, glutamine, histidine, methionine, phenylalanine, proline, threonine and tyrosine. The difference between two consecutive seasons was evaluated, as well as the influence of fruit maturity of ‘Page’ collected in two periods of 2015. The analytical methodology was validated. The concentration of the compounds through principal component analysis, separated well apart the three cultivars in both harvests, showing a particular profile for each of them. When comparing mature and immature cultivar ‘Page’, the amino acids with higher levels in mature samples were histidine, asparagine, glutamine and glutamic acid. The profiles were different due to genetic diversity, and the climatic conditions. These results add value to citric production.

]]> Rezende, Sofía]]> Banchero, Sabrina]]> Migues, Ignacio]]> Cesio, María Verónica]]> Rivas, Carloss Fernando]]> Heinzen, Horacio]]> Besil, María Natalia]]> Información sobre Derechos de Autor

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]]> Floral food resources for Apis mellifera (Hymenoptera: Apidae) in a mountain forest area in Uruguay]]> Díaz, Rosana Yacqueline]]> Niell, Silvina]]> Cesio, María Verónica]]> Heinzen, Horacio]]> Agrociencia, v. 25, no. 2, 2021. -- pp. 1-16

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Listening to the voices of adolescents for the design of strategies to promote healthy eating : an exploratory study in a Latin American country.]]> Objective: To explore adolescents’ views about the foods they consume and to identify their ideas about strategies to encourage healthier eating habits.
Design: Individual questionnaires based on open-ended questions and group discussions (6–8 participants) were used to address the objectives. Data were analysed using content analysis based on deductive-inductive coding.
Setting: Montevideo and its metropolitan area (Uruguay, Latin America).
Participants: Totally, 102 adolescents (aged between 11 and 15 years, 52 %female) recruited at two educational institutions.
Results: Adolescents reported frequently consuming ultra-processed products and fast food although they were perceived as bad for their health, whereas they reported an infrequent consumption of fruits and vegetables. Multifaceted strategies
to promote healthy eating habits emerged from adolescents’ accounts, including public awareness campaigns, nutrition education programmes, nutrition label standards and regulations, and changes in food availability and affordability.
Conclusions: Results from the present work suggest that co-creation with adolescents may be an effective way to inform the development of strategies to promote healthier eating habits. The strategies suggested by adolescents were mainly focused on behaviour change communication, who emphasised the importance of social media and the involvement of celebrities and influencers. The need for educational and communication strategies to raise awareness of the social and environmental drivers of eating patterns among adolescents was identified.

]]> Ares, Gastón]]> Antúnez, Lucía]]> Alcaire, Florencia]]> Vidal, Leticia]]> Bove, Isabel]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Consumer attitudes to vertical farming (indoor plant factory with artificial lighting) in China, Singapore, UK, and USA : a multi-method study.]]> Major changes are needed both with regard to what we eat and how food is produced. The latter is the focus of the present research, specifically the rise of controlled environment agriculture. In this context, empirical research is presented on consumer attitudes to vertical farming (VF) (i.e., indoor plant factory with artificial lighting), conducted in four countries (USA, UK, Singapore, and China) using online surveys (637–683 participants per country with matched gender and age group distributions). A multi-method research approach was
used, including a novel methodology of text highlighting, which requires that participants read a descriptive text about VF with mentions of pros and cons and use highlighter functions to select aspects of the text that they ‘like’ and ‘dislike’. Based on the information provided in the text, attitudes towards VF were largely positive in the four countries. The characteristics of VF that aligned with the United Nations Sustainable Development Goals were identified as key drivers of positive attitudes (i.e., higher yield, reduction of carbon emissions, and securing
access to food). On the other hand, high energy use and premium prices contributed to negative attitudes about VF. Although the majority of participants responded to the text with an overall positive attitude towards VF, there were smaller groups of participants in every country who expressed a negative or neutral/ambivalent attitude. These between-segment differences were larger than cross-cultural differences, although the latter did exist, particularly for selected aspects of VF. For example, Chinese participants tended to be the least negative about the use of robots to help planting and harvesting. Future research is needed to understand consumer responses to aspects VF not covered in the text (e.g., powering VF with renewable energy, product range), and consumer insights about VF should be sought in other countries.

]]> Ares, Gastón]]> Ha, Brigit]]> Jaeger, Sara R.]]> Información sobre Derechos de Autor

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