patterns moderate such effect. Eight samples of liquid hand soap were obtained following a full factorial design with three 2-level variables: fragrance (herbal vs. fruity), color (green vs. orange), and transparency (transparent vs. pearly).
Samples were evaluated by 104 female participants, who rated their liking and used a
check-all-that-apply question to describe the samples in terms of sensory characteristics,
expected functional aspects of the product, and conceptual associations. Results showed that appearance and transparency did not influence consumer liking. However, fragrance had a significant effect on consumer liking, expectations and conceptual associations of liquid hand soap. The effect of fragrance on expectations and
conceptual associations depended on consumer liking of the fragrance. In addition, pearly appearance did not influence liking but had a significant effect on consumer expectations regarding functional aspects of the liquid hand soap.]]> Parente, Emma]]> Ares, Gastón]]>

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La legislación uruguaya protege el derechode autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> Sulfur precursor and citric acid effect on SnS2 nanoparticles and their influence on the photodegradation activity of selected organic compounds]]> nanoparticles exhibited faster kinetics for methyl orange (MO) degradation, Kapp = 0.0102 min−1, and 0.029 min−1, respectively. We proved that SnS2 is capable of breaking the azo bond of methyl orange by direct reduction. Furthermore, our analyses indicate that SnS2 nanoparticles do not degrade atrazine and imazapic, but the photocatalytic route of metribuzin competed with photolysis, resulting in a particular transformation product that was not obtained with light irradiation only. We demonstrated that SnS2 nanoparticles have high bond selectivity for azo breaking. Furthermore, they represent an advance for the development of designed materials (such as heterostructures), where the properties of SnS2 can be tuned.]]> Oreggioni, Daniela A.]]> Pérez Parada, Andrés]]> Aguiar, Ivana]]> Colazzo, Marcos]]> Pareja, Lucía]]> De León, María Andrea]]> Bentos Pereira, Heinkel]]> Pérez Barthaburu, María Eugenia]]>

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]]> High performance Ni-catalysts supported on rare-earth zirconates (La and Y) for hydrogen production through ethanol steam reforming. Characterization and assay]]> Musso, Mauricio]]> Cardozo, Alejandra]]> Romero, Mariano]]> Faccio, Ricardo]]> Segobia, Dario]]> Apesteguía, Carlos]]> Bussi, Juan]]>

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]]> 10.1016/j.cattod.2021.07.001 ]]> Relationship between Baccharis dracunculifolia DC. and B. microdonta DC. (Asteraceae) by Their Different Seasonal Volatile Expression]]> Baccharis dracunculifolia DC. and Baccharis microdonta DC. (Asteraceae) are woody species morphologically similar growing in Uruguay, where not taxonomists people often confuse them in field conditions. As the essential oil of B. dracunculifolia (‘vassoura’ oil) is highly prized by the flavor and fragrance industry, the correct differentiation of the two species is a key factor in exploiting them profitably and reasonably. To differentiate both Baccharis species, in this work their volatile expression profiles were studied as an alternative tool to
determine authenticity and quality. Volatile organic compounds (VOCs) were monthly extracted during an entire year from aerial parts of wild populations by simultaneous distillation extraction (SDE), and studied by gas chromatography/mass spectrometry (GC/MS; identification) and conventional gas chromatography (GC-FID; component abundances determination). Enantioselective gas chromatography/mass spectrometry (Es-GC/MS) was applied in the search of parameters able to ensure genuineness of each species extract. Qualitative VOCs profiles were found to be similar for both species, being β-pinene, limonene, spathulenol, caryophyllene oxide, and viridiflorol the main components. However, the abundance of those VOCs were two to ten times higher in B.
dracunculifolia than in B. microdonta during the year of study. These Baccharis spp. showed species-specific patterns of VOCs expression according to the seasonality, and interestingly, oxygenated compounds (transpinocarveol and myrtenal) increased their abundances at full-flowering stages. The enantiomeric distribution of selected monoterpenes (α- and β-pinenes, limonene, linalool, terpinen-4-ol, and α-terpineol) presented differential values for both Baccharis spp., meaning that Es-GC might be a useful tool for differentiating chemically both species in Uruguay for genuineness determination purposes.

]]> Minteguiaga, Manuel]]> González, Andrés]]> Catalán, César A. N.]]> Dellacassa, Eduardo]]> Información sobre Derechos de Autor

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]]> 10.1002/cbdv.202100064 ]]> Ultrastructural characterization of the tegument in protoscoleces of Echinococcus ortleppi ]]> Cystic echinococcosis is a globally distributed zoonosis caused by cestodes of the Echinococcus granulosus sensu lato (s.l.) complex, with Echinococcus ortleppi mainly involved in cattle infection. Protoscoleces show high developmental plasticity, being able to differentiate into either adult worms or metacestodes within definitive or intermediate hosts, respectively. Their outermost cellular layer is called the tegument, which is important in determining the infection outcome through its immunomodulating activities. Herein, we
report an in-depth characterization of the tegument of E. ortleppi protoscoleces performed through a combination of scanning and transmission electron microscopy techniques. Using electron tomography, a three-dimensional reconstruction of the tegumental cellular territories was obtained, revealing a novel structure termed the ‘tegumental vesicular body’ (TVB). Vesicle-like structures, possibly involved in endocytic/exocytic routes, were found within the TVB as well as in the parasite glycocalyx, distal cytoplasm and close inner structures. Furthermore, parasite antigens (GST-1 and AgB) were unevenly localised within tegumental structures, with both being detected in vesicles found within the TBV. Finally, the presence of host (bovine) IgG was also assessed, suggesting a possible endocytic route in protoscoleces. Our data forms the basis for a better understanding of E. ortleppi and E. granulosus s.l. structural biology.

]]> Miles, Sebastián]]> Magnone, Javier]]> García Luna, Joaquín]]> Ancarola, María Eugenia]]> Cucher, Marcela]]> Dematteis, Sylvia]]> Frischknecht, Friedrich]]> Cyrklaff, Marek]]> Mourglia Ettlin, Gustavo]]> Información sobre Derechos de Autor

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]]> 10.1016/j.ijpara.2021.05.004 ]]> Purification of protein from Arthrospira platensis using aqueous two-phase system associate with membrane separation process and evaluation of functional properties ]]> Arthrospira platensis has stood out, especially due to its high protein content and its potential for food application. However,
the exploration of protein concentration techniques of this microalgae still presents a great challenge. Thus, the objective
of this study was to evaluate the purification of A. platensis proteins by applying two different aqueous two-phase systems
(ATS) composed of potassium phosphate and ethanol, and sodium citrate and ethanol, in addition to the association with
membrane separation process (MSP), and the characterization of the fractions obtained. ATS under the optimised conditions
(19% potassium phosphate and 30% ethanol, and 19.5% sodium citrate and 29% ethanol) resulted in 1.27 and 1.31 purity, and 77.45% and 78% recovery, respectively. The sodium citrate and ethanol ATS showed significantly greater purity; thus, the upper phase was submitted to MSP. The association of ATS and MSP increased the recovery (~ 87%) and the purity (1.5). The top phase of ATS showed higher protein content (61%) than the bottom (5.9%), confirming the protein preference to the top. When the MSP was evaluated, the protein was increased about 8.0%, and antioxidant activity was kept (42 and 40% to top phase and ATS/MSP concentrate, respectively). Emulsions formed by ATS top phase showed a lower droplet size (33.69 μm D4.3 and 6.02 μm D3.2), and a significantly lower degree of overall destabilization (0.04 × 10–
6 s−1 kh and 3.54 × 10–3 s−1 ks) than those stabilized with bottom phase and MSP/ATS concentrate. ATS associated with membrane process showed efficiency in the concentration A. platensis protein, being able to be an alternative to be used in industrial systems.

]]> Lupatini Menegotto, Anne Luize]]> Fernandes, Ilizandra Aparecida]]> Bucior, Danieli]]> Pinto Balestieri, Bianca]]> Colla, Luciane Maria]]> Abirached, Cecilia]]> Franeschi, Elton]]> Steffens, Juliana]]> Valduga, Eunice]]> Información sobre Derechos de Autor

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]]> 10.1007/s10811-021-02505-0 ]]> Development of tropical mixed juice with low added-sugar content: sensory and nutritional aspects]]> The aim of the present study was to develop a mixed tropical fruit juice of cashew apple, acerola and melon with low added-sugar content considering sensory and nutritional aspects. Five formulations were developed varying the concentration of the different fruits. A total of 172 consumers rated their overall liking using a 9-point hedonic scale and described their sensory characteristics using a Check-all-that-apply question. In addition, the functional properties of the juices were evaluated by analyzing vitamin C, total phenolic compounds and antioxidant capacity. Results showed that the addition of sugar and fruit composition affected the sensory characteristics and consumer liking of the formulations. Samples without added sugar and with higher melon concentration showed lower liking scores (p<0.05). In addition, the highest concentration of acerola had a positive effect on nutritional characteristics. The formulation with 50% of fruit pulp (60% of cashew apple,
30% of acerola and 10% of melon), 47% of water, and 3% of added sugar achieved the best results. The effect of aroma on sweetness perception was investigated by adding identical to natural aromas of melon, apple and pineapple to the selected formulation. However, aroma did not significantly modify sweetness perception, evaluated using a rate-all-that-apply question.

]]> Araujo Martins, Inayara Beatriz]]> Carvalho Costa Barbosa, Isadora de]]> Rosenthal, Amauri]]> Ares, Gastón]]> Deliza, Rosires]]> Información sobre Derechos de Autor

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]]> 10.1177/10820132211020844 ]]> Application of high hydrostatic pressure for the reduction of STEC on raw ground beef patties and its impact on physicochemical properties: pH and color]]> This study on raw ground beef patties evaluated: resistance of STEC strains inoculated at 6 log CFU/g (O157:H7, O26, O45, O103, O111, O121, and O145) subjected to 350, 450 and 600 MPa, for 5 min at 10 ◦C; effectiveness of pressure for initial loads (2, 3 and 4 log CFU/g) of O157:H7; and general impact of high hydrostatic pressure (HHP) on raw patties. For 600 MPa treatments and 6 log CFU/g load of STEC, ~5 log CFU/g reductions were obtained with all strains. For 450 MPa, differences in baroresistance among strains became evident. When inoculating O157:H7 at different loads, counts after 600 MPa fell below quantification limits, though virulence genes were detected for the two highest loads. Additionally, HHP reduced native aerobic microbiota to <1 log CFU/g and slightly affected a* and b* color values. Montecarlo simulation was used to estimate potential initial counts of STEC that allow compliance with existing regulatory limits after applying HHP, showing that absence of STEC studied can be achieved in 65 g of patties provided initial loads are ~2 log CFU/g. Finally, HHP
treatments at 600 MPa and mild temperatures can be considered a valid non-thermal processing technology to
achieve 5 log CFU/g reductions.

]]> Martínez Barnié, Inés]]> Mussio, Paula]]> Jorcin, Santiago]]> Rajchman, Mikaela]]> López Pedemonte, Tomás]]> Información sobre Derechos de Autor

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]]> 10.1016/j.lwt.2021.112126 ]]> Exchange coupling in a thiocyanato-bridged copper(II) chain: computational approach to magnetostructural correlations]]> value]]> Martínez, Lorena]]> Veiga, Nicolás]]> Bazzicalupi, Carla]]> Bianchi, Antonio]]> LLoret, Francesc]]> Kremer, Carlos]]> Chiozzone, Raúl]]> Información sobre Derechos de Autor

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]]> 10.1016/j.poly.2021.115406 ]]> Assessing SIRAH’s capability to simulate intrinsically disordered proteins and peptides]]> The challenges posed by intrinsically disordered proteins (IDPs)
to atomistic and coarse-grained (CG) simulations are boosting efforts to develop and reparametrize current force fields. An assessment of the dynamical behavior of IDPs’ and unstructured peptides with the CG SIRAH force field suggests that the current version achieves a fair description of IDPs’ conformational flexibility. Moreover, we found a remarkable capability to capture the effect of point mutations in loosely structured peptides.

]]> Klein, Florencia]]> Barrera, Exequiel E]]> Pantano, Sergio]]> American Chemical Society]]> Información sobre Derechos de Autor

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]]> Fernández Werner, Luciana]]> Bechthold, P.]]> Jiménez, M. J.]]> Jasen, P. V.]]> Faccio, Ricardo]]> Gonzalez, E. A.]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1016/j.apsusc.2021.150679 ]]> Adsorption of organic molecules on graphene and fluorographene: an unresolved discrepancy between experiment and theory]]> Herein, we studied the adsorption of 15 organic and three polar molecules onto pristine and fluorinated graphene. Two levels of fluorination were selected, CF fluorographene for which all carbon atoms are fluorinated and C4F fluorinated graphene, which maximizes the number of Clar sextets. Five different density functionals were employed: M06-L, M06-2X, PBE-D2 and PBE-D3BJ. In contrast with recent experimental investigations, we found that the adsorption energies for organic molecules onto perfect graphene are significantly lower than those determined for fluorographene. This situation is exacerbated when enthalpic and free energy corrections are included. However, a different scenario was found for polar molecules such as H2O, H2O2 and HF. Partial fluorination following a C4F pattern leads to significantly lower adsorption energies for these three molecules, and the superhydrophilicity
of C4F is maintained after thermodynamic corrections are included. In fact, even the removal of a single fluorine atom results in a dramatic change in the adsorption energy of water on fluorographene. We discuss the performance of the
density functionals assayed and the importance of using finite and infinite models. This work demonstrates that it is possible to adjust the adsorption energy of a given polar molecule onto graphene by controlling the fluorination level and in doing so,
that partially fluorinated graphene models may open new avenues for the separation of molecules with different polarity.

]]> Iribarne, Federico]]> Denis, Pablo A.]]> Información sobre Derechos de Autor

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]]> Heffner, Herman
]]> Faccio, Ricardo]]> López Corral, Ignacio]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1016/j.apsusc.2021.149479 ]]> Detection of SOF2 and SO2F2 through aluminium nitride nanosheets: a DFT study]]> Detection of sulfuryl fluoride (SO2F2) and thionyl fluoride (SOF2) can be used to find out the degradation of sulfur hexafluoride (SF6), indicating gas insulation switchgear equipment failures. In the present work, we study through first-principle DFT calculations the SOF2 and SO2F2 adsorption on aluminium nitride nanosheets (AlNNS), with the aim of to evaluate the performance of this nanomaterial as gas sensor. First, we performed an energetic, geometric and bonding analysis, obtaining considerable adsorption energy values for SO2F2 and SOF2 (−1.63 and −1.93 eV, respectively), which suggests these gases are chemisorbed on the AlN surface.
Furthermore, certain SeF bonds were broken during adsorption, while new AleF and SeN overlaps were developed. Simultaneously, an important weakening was observed for AleN bonds close to the adsorption site. The adsorption selectivity of AlNNS toward SO2F2 and SOF2 was also studied, noting that other molecules normally present in air show a lower reactivity.
Band structure and electronic density of states were realized in order to evaluate the sensing capacity of AlNNS surface. The obtained plots show a notorious narrowing of the band gap after gas adsorption, particularly in the case of SOF2, so an important change in the conductivity of AlNNS could be expected during gas exposition. Charge density analysis reveals an important charge transfer from AlNNS to both adsorbed gases, which suggest that this nanomaterial could behave as an n-type semiconductor. These results seem to indicate that
AlNNS could act with good sensibility and selectivity for SOF2 and SO2F2 detection, showing high potential as gas sensing material.

]]> González Fá, Alejandro J.]]> Faccio, Ricardo]]> López Corral, I.]]> Información sobre Derechos de Autor

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]]> González Arbildi, Joaquín]]> Cavelli, Matias]]> Castro Zaballa, Santiago]]> Mondino, Alejandra]]> Tort, Alejandro B. L.]]> Rubido, Nicolás]]> Carrera, Ignacio]]> Torterolo Minetti, Pablo Daniel]]> American Chemical Society]]> Información sobre Derechos de Autor

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]]> 10.1021/acsptsci.0c00164 ]]> Efficient access to the Iboga Skeleton: optimized procedure to obtain voacangine from Voacanga africana root bark]]> Iboga alkaloids are a group of monoterpenoid indole alkaloids with promising and intriguing biological activities. Ibogaine is the representative member of the series and has become widely known as a potent atypical psychedelic with promising effects to treat substance use disorder. Nowadays, an efficient and scalable enantioselective total synthesis of ibogaine and related iboga alkaloids is still lacking, so direct extraction from natural sources or semi-synthetic schemes are the methods of choice to obtain them in a preparative scale. In particular, ibogaine can be obtained either by a low yielding direct isolation from Tabernanthe iboga or using a semi-synthetic procedure from voacangine, an iboga alkaloid occurring in a higher yield in the root bark of Voacanga africana. In this work, we describe an optimized process to obtain voacangine from V. africana root bark as a precursor of the iboga scaffold. Using a direct acetone-based extraction procedure (0.5 kg of root bark), voacangine was isolated in ∼0.8% of root bark dried weight, while the major alkaloids isolated from the bark were identified as iboga-vobasinyl dimers (∼3.7%) such as voacamine and voacamidine. Since these alkaloids contain the voacangine moiety in their structure, the cleavage of the dimers was further optimized, affording an extra amount of voacangine in ∼50% isolated molar yield. In this manner, the total amount of voacangine obtained by application of the whole procedure to the plant material (extraction and dimer cleavage) could almost duplicate the content originally found in the root bark.

]]> González, Bruno]]> Fagúndez, Catherine]]> Peixoto de Abreu Lima, Alejandro]]> Suescun, Leopoldo]]> Sellanes, Diver]]> Seoane, Gustavo A.]]> Carrera, Ignacio]]> Información sobre Derechos de Autor

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]]> Garmendia, Gabriela]]> Alvarez, Angie]]> Villarreal, Romina]]> Martínez Silveira, Adalgisa]]> Wisniewski, Michael]]> Vero, Silvana]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1007/s11274-021-03112-4 ]]> Polyreactive antibodies as potential humoral biomarkers of host resistance to cystic echinococcosis]]> Polyreactive antibodies (pAb) bind to a broad range of unrelated structures, providing hosts with functional components able to rapidly recognize and protect against different pathogens. However, their roles against helminth parasites are still unexplored. Here, pAb profiles were analysed in cystic echinococcosis (CE), a zoonosis caused by the cestode Echinococcus granulosus sensu lato. Levels of anti-DNP (2,4-dinitrophenyl-hapten) antibodies were measured as a surrogate parameter of pAb in different biological settings. Firstly, levels of serum and peritoneal pAb were measured during early experimental secondary CE, using both high (Balb/c) and low (C57Bl/6) susceptible mouse strains. Serum pAb mostly differed in normal mice, being pAb levels of IgG subclasses with poor anti-parasite activities predominant in Balb/c animals.
Conversely, peritoneal pAb isotypes/subclasses with efficient anti-parasite activities predominated in normal and infected C57Bl/6 mice. Secondly, sera from potentially resistant patients, susceptible individuals and healthy donors were analysed, showing higher pAb levels of the IgA and IgG—particularly IgG1—isotypes in potentially resistant individuals compared to control groups. Finally, since remarkable differences were observed in pAb profiles according to the intrinsic host susceptibility to the infection, we proposed here that pAb might be considered as potential humoral biomarkers
for host resistance to CE.

]]> García Luna, Joaquín]]> Magnone, Javier]]> Miles, Sebastián]]> López Santurio, Camila]]> Dematteis, Sylvia]]> Mourglia Ettlin, Gustavo]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1111/pim.12802 ]]> Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate]]> Haemonchus contortus, a blood-sucking parasite of small ruminants, produces very important economic losses in the productive sector. This abomasum parasite has become resistant to most commercial drugs worldwide, and alternatives to fight this problem are urgently needed. Essential oils (EO) are a complex mixture of volatile secondary metabolites, composed mainly by terpenoids and phenolic compounds, from plants that have several pharmacological properties, including anthelmintic activity. Particularly, citrus peel is a source of cold-pressed EO, where limonene is its major component, and can be used as an additional food component for ruminants. The
aim of the present work was to determine the in vitro anthelmintic activity of EO from Citrus bergamia (EOB), C. x
paradisii (EOG) and limonene against the benzimidazole-susceptible Kirby isolate of H. contortus, using the egg hatch test (EHT) and the exsheathed third stage larval motility test (XLMT) using a WMicroTracker equipment. Albendazole (ABZ) and monepantel (MON) were used as positive controls. The 50% inhibitory concentrations (IC50) in XLMT were 8.77 and 13.88 μg/ml for EOB and EOG respectively, after an incubation of 72 h. An interesting observation on XLMT resulted when the positive controls were tested on the same plate, but in different well of the EOB. The volatile components of the EO significantly influenced (P < 0.05) the percentage of larval motility, reducing values from 66.9 to 19.6% for ABZ, and from 72.8 to 33.7% for MON, when comparing the activity of positive controls in a control plate without EO. The in vitro anthelmintic activity of EOB and EOG shows that they could be interesting candidates for nematode control. It is still necessary additional studies
against the adult stage of H. contortus in efficacy trials in infected animals to validate their anthelmintic activity.

]]> Garbin, Vivien Patricia]]> Munguía, Beatriz]]> Saldaña, Jenny Carolina]]> Deschamps, Cícero]]> Raupp Cipriano, Roger]]> Beltrao Molento, Marcelo]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1016/j.actatropica.2021.105869 ]]> In Vitro bioaccessibility of bioactive compounds from citrus pomaces and orange pomace biscuits ]]> The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces (mandarin varieties Clemenule and Ortanique and orange varieties Navel and Valencia). The effects of the baking process on their bioaccessibility was also assessed. Samples of pomaces and biscuits containing them as an ingredient were digested, mimicking the human enzymatic oral gastrointestinal digestion process, and the composition of the digests were analyzed. UHPLC-MS/MS results of the citrus pomaces flavonoid
composition showed nobiletin, hesperidin/neohesperidin, tangeretin, heptamethoxyflavone, tetramethylscutellarein, and naringin/narirutin. The analysis of the digests indicated the bioaccessibility of compounds possessing antioxidant [6.6–11.0 mg GAE/g digest, 65.5–97.1 mol Trolox Equivalents
(TE)/g digest, and 135.5–214.8 mol TE/g digest for total phenol content (TPC), ABTS, and ORAC-FL methods, respectively; significant reduction (p < 0.05) in Reactive Oxygen Species (ROS) formation under tert-butyl hydroperoxide (1 mM)-induced conditions in IEC-6 and CCD-18Co cells when pre-treated with concentrations 5–25 g/mL of the digests], anti-inflammatory [significant reduction (p < 0.05) in nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages], and antidiabetic (IC50 3.97–11.42 mg/mL and 58.04–105.68 mg/mL for -glucosidase and -amylase inhibition capacities) properties in the citrus pomaces under study. In addition, orange pomace biscuits with the nutrition claims “no-added sugars” and “source of fiber”, as well as those with good sensory quality (6.9–6.7, scale 1–9) and potential health promoting properties, were
obtained. In conclusion, the results supported the feasibility of citrus pomace as a natural sustainable source of health-promoting compounds such as flavonoids. Unfractionated orange pomace may be employed as a functional food ingredient for reducing the risk of pathophysiological processes linked to oxidative stress, inflammation, and carbohydrate metabolism, such as diabetes, among others.

]]> Fernández Fernández, Adriana Maite]]> Dellacassa, Eduardo]]> Nardin, Tiziana]]> Larcher, Roberto]]> Gámbaro, Adriana]]> Medrano Fernandez, Alejandra]]> del Castillo, María Dolores]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.3390/molecules26123480 ]]> Antioxidant, antidiabetic, and antiobesity properties, TC7-Cell cytotoxicity and pptake of Achyrocline satureioides (Marcela) conventional and high pressure-assisted extracts]]> The growing incidence of non-communicable diseases makes the search for natural sources of bioactive compounds a priority for such disease prevention/control. Achyrocline satureioides
(‘marcela’), a plant rich in polyphenols and native to Brazil, Uruguay, Paraguay, and Argentina, could be used for this purpose. Data on its antidiabetic/antiobesity properties and cellular uptake of bioactive compounds are lacking. The potentiality of non-thermal technologies such as highhydrostatic
pressure (HP) to enhance polyphenol extraction retains attention. Thus, in the present study aqueous and ethanolic marcela extracts with/without assisted-HP processing were chemically characterized and assessed for their in vitro antioxidant capacity, antidiabetic and antiobesity activities, as well as cellular cytotoxicity and uptake on intestinal cell monolayers (TC7-cells, a clone of Caco-2 cells). Aqueous and ethanolic conventional extracts presented different polyphenolic
profiles characterized mainly by phenolic acids or flavonoids, respectively, as stated by reverse phase-high-performance liquid chromatography (RP-HPLC) analyses. In general, ethanolic extracts presented the strongest bioactive properties and HP had none or a negative effect on in vitro bioactivities comparing to conventional extracts. TC7-cell viability and cellular uptake demonstrated in conventional and HP-assisted extracts, highlighted the biological effects of marcela bioactive
compounds on TC7-cell monolayers. TC7-cell studies showed no HP-induced cytotoxicity. In sum, marcela extracts have great potential as functional ingredients for the prevention/treatment of chronic diseases such as diabetes.

]]> Fernández Fernández, Adriana Maite]]> Dumay, Eliane]]> Lazennac, Françoise]]> Migues, Ignacio]]> Heinzen, Horacio]]> Lema, Patricia]]> López Pedemonte, Tomás]]> Medrano Fernandez, Alejandra]]> Información sobre Derechos de Autor

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]]> Favre, Sofia]]> Ariosa, Daniel]]> Yelpo, Carla]]> Mazini, Marcos]]> Faccio, Ricardo]]> Información sobre Derechos de Autor

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]]> The present work addresses some fundamental aspects in the preparation of protein-conjugated gold nanoparticles, in order to ensure an appropriate final product. Ten broadly available and/or easy to implement analytical tools were benchmarked and compared in their capacity to provide reliable and conclusive information for each step of the procedure. These techniques included transmission electron microscopy, UV/VIS spectroscopy, dynamic light scattering, zeta-potential, Fourier-transformed infrared spectroscopy, colloidal stability
titration, end-point colloidal stability analysis, cyclic voltammetry, agarose gel electrophoresis and size-exclusion
chromatography (SEC). Four different proteins widely used as adaptors or blocking agents were tested, together with 13 nm gold nanoparticles containing different surface chemistries. Among all tested techniques, some of the least popular among nanomaterial scientists probed to be the most informative, including colloidal stability, gel electrophoresis and SEC; the latter being also an efficient purification procedure. These three techniques provide low-cost, low time consuming, sensitive and robust ways to assess the success of the nanoparticle bioconjugation steps, especially when used in adequate combinations.

]]> Fagúndez, Pablo]]> Botasini, Santiago]]> Tosar, Juan Pablo]]> Méndez, Eduardo]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> Estevez, Martín]]> Mombrú, Dominique]]> Romero, Mariano]]> Fernández Werner, Luciana]]> Faccio, Ricardo]]> Mombrú, Álvaro W.]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

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]]> 10.1016/j.apsusc.2020.147733 ]]> Optical, electrical and structural properties of Fe doped sodium titanate nanostructures]]> In this work we present the synthesis, optical and electrical characterization of nanoparticles of iron doped anatase and iron doped sodium titanate high aspect ratio nanostructures (Na2Ti2O5⋅H2O). Our study focused mainly in the study of the electronical structure and the derived properties of the dielectric function by DFT (Density Functional Theory) means and their relationship with experimental measurements of optical band gap and electric impedance spectroscopy. Also, structural and morphological characterizations of the systems were carried on by X-ray powder diffraction, high resolution transmission electron microscopy and Raman spectroscopy.
It was possible for the doped nanostructures to observe a slight red shift in the band gap of about 0.2 eV and an order of magnitude decrease in the global resistance in the doped system.

]]> Estevez, Martín]]> Fernández Werner, Luciana]]> Campos Plá Cid, Cristiani]]> Pelegrini, Silvia]]> Pasa, André A.]]> Faccio, Ricardo]]> Mombrú, Álvaro W.]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> 10.1016/j.apsusc.2021.149534

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