Method optimization and validation for multi-class residue analysis in turmeric ]]> Turmeric (Curcuma longa L.) is an economically important food and medicinal plant that grows in tropical and subtropical regions consumed worldwide. Turmeric is a difficult matrix for residue/contaminant analysis due to the high content of potentially interfering polyphenols (curcuminoids) and essential oils with physicochemical properties similar to target analytes. To solve this problem, a simple and straightforward sample preparation protocol based on ethyl acetate extraction and dispersive solid phase clean-up, followed by gas and liquid
chromatography coupled to tandem mass spectrometry analysis, was optimized and validated, after selecting a
representative blank material using gas chromatography coupled to ion mobility spectrometry and multivariate
data analysis. Method validation was performed according to Codex guidelines for 67 pesticide residues, including 12 persistent organic pollutants, 4 aflatoxins and 3 dyes at 0.01, 0.02 and 0.05 mg kg

]]> Maestroni, Britt]]> Besil, Natalia]]> Rezende, Sofía]]> Liang, Ying]]> Gerez, Natalia]]> Karunarathna, Nuwan]]> Islam, Marivil]]> Heinzen, Horacio]]> Cannavan, Andrew]]> Cesio, María Verónica]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

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]]> Dinuclear complex of Cu(II) containing chloride and methoxide as bridging ligands: a new crystal structure and DFT calculations.]]> A new crystal structure for complex [Cu2Cl2(bipy)2(μ-Cl)(μ-CH3O)] was obtained. X-ray diffraction data showed dinuclear complexes with metal-ions seated on two symmetrically related pentacoordinated environment, in which, a trigonal bipyramidal
(TBPY-5) coordination polyhedra was observed. While chloride was detected as monodentate and as a bridging-ligand,
the diimine unit and a bridging oxygen from methoxide occupied the remaining positions. The crystal structure accounted for dinuclear complexes interacting to each other via H-bonds involving C–H groups of bipy and monodentate chloride anions of an adjacent molecule. Theoretical studies were conducted by DFT methods employing B3LYP in combination with 6-31G(d). Larger basis sets (6-311G(d) and cc-pVDZ) were taken into account to assess the performance of B3LYP/6-31G(d) in dealing with equilibrium geometry and population analysis. This standard methodology showed to be a reasonable performer to reproduce geometric parameters keeping the computational cost very low.

]]> Luzardo, Florencia]]> Álvarez, Natalia]]> Soca Rosas, Carolina]]> Kremer, Carlos]]> Stucchi de Camargo, Andrea S.]]> Gancheff, Jorge S.]]> Información sobre Derechos de Autor

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]]> Unraveling factors affecting consumers' liking of novel Uruguayan mandarins]]> Lado Lindner, Joanna.]]> Moltini, Ana Inés]]> Pintos, Pedro]]> Luque, Eleana]]> Goncalvez, Lucía]]> Alcaire, Florencia]]> Ares, Gastón]]> Rivas, Fernando]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.31285/AGRO.25.540]]> Effect of Saccharomyces and non-Saccharomyces native yeasts on beer aroma compounds ]]> Recently, the increase in microbreweries and the consequent production of craft beers have reached exponential growth. The interest in non-conventional yeasts for innovation and a unique selling feature in beer fermentation is increasing. This work studied the autochthonous Saccharomyces and non Saccharomyces yeasts, isolated from various food sources, with the ability to modify and improve the fermentative and aromatic profiles during alcoholic fermentation. The ability to ferment maltose and produce desirable aroma compounds were considered as the key characters for the screening selection. A synthetic beer wort was developed for this purpose, to simulate beer wort composition.

A total of forty-seven yeast strains belonging to different genera were analysed according to their fermentation profile, volatile compounds production and sensory analysis. Three native strains of Saccharomyces cerevisiae, Zygoascus meyerae and Pichia anomala were selected to evaluate their aromatic profile in single and mixed fermentations. The strains produced 4-vinylguaiacol, β-phenylethyl alcohol, and isoamyl alcohol at levels significantly above the sensory threshold, making them interesting for wheat and blond craft beer styles. The native Hanseniaspora vineae was also included in a co-fermentation treatment, resulting in a promising yeast to produce fruity beers.

]]> Larroque, M. N.]]> Carrau, F.]]> Fariña, L.]]> Boido, E.]]> Dellacassa, E.]]> Medina, K.]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1016/j.ijfoodmicro.2020.108953]]> Rapid freshwater discharge on the coastal ocean as a mean of long distance spreading of an unprecedented toxic cyanobacteria bloom]]> Cyanobacterial toxic blooms are aworldwide problem. The Río de la Plata (RdlP) basinmakes up about one fourth of South America areal surface, second only to the Amazonian. Intensive agro-industrial land use and the construction of dams have led to generalized eutrophication ofmain tributaries and increased the intensity and duration of cyanobacteria blooms. Herewe analyse the evolution of an exceptional bloomat the lowRdlP basin and Atlantic coast during the summer of 2019. A large array of biological, genetic,meteorological, oceanographic and
satellite data is combined to discuss the driving mechanisms. The bloomcovered thewhole stripe of the RdlP estuary and the Uruguayan Atlantic coasts (around 500 km) for approximately 4 months. It was caused by the Microcystis aeruginosa complex (MAC), which produces hepatotoxins (microcystin). Extreme precipitation in the upstreamregions of Uruguay and Negro rivers' basins caused highwater flows and discharges. The evolution of meteorological and oceanographic conditions as well as the similarity of organisms' traits in the affected area
suggest that the bloom originated in eutrophic reservoirs at the lower RdlP basin, Salto Grande in the Uruguay river, and Negro river reservoirs. High temperatures and weak Eastern winds prompted the rapid dispersion of the bloom over the freshwater plume along the RdlP northern and Atlantic coasts. The long-distance rapid drift allowed active MAC organisms to inoculate freshwater bodies from the Atlantic basin, impacting environments relevant for biodiversity conservation. Climate projections for the RdlP basin suggest an increase in precipitation and river water flux, which, in conjunction with agriculture intensification and dams' construction, might turn this extraordinary event into an ordinary situation.

]]> Kruk, Carla]]> Martínez, Ana]]> Martínez de la Escalera, Gabriela]]> Trinchin, Romina]]> Manta, Gastón]]> Segura, Angel M.]]> Piccini, Claudia]]> Brena, Beatriz]]> Yannicelli, Beatriz]]> Fabiano, Graciela]]> Calliari, Danilo]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1016/j.scitotenv.2020.142362]]> CUTie2: the attack of the cyclic nucleotide sensor clones]]> The detection of small molecules in living cells using genetically encoded FRET sensors has revolutionized our understanding of signaling pathways at the sub-cellular level. However,
engineering fluorescent proteins and specific binding domains to create new sensors remains challenging because of the difficulties associated with the large size of the polypeptides involved, and their intrinsically huge conformational variability. Indeed, FRET sensors’ design still relies on vague structural notions, and trial and error combinations of linkers and protein modules. We recently designed a FRET sensor for
the second messenger cAMP named CUTie (Cyclic nucleotide Universal Tag for imaging experiments), which granted sub-micrometer resolution in living cells. Here we apply a combination of sequence/structure analysis to produce a new-generation FRET sensor for the second messenger cGMP based on Protein kinase G I (PKGI), which we named CUTie2. Coarse-grained molecular dynamics simulations achieved an exhaustive
sampling of the relevant spatio-temporal coordinates providing a quasi-quantitative prediction of the FRET efficiency, as confirmed by in vitro experiments. Moreover, biochemical characterization showed that the cGMP binding module maintains virtually the same affinity and selectivity for its ligand thant the full-length protein. The computational approach proposed here is easily generalizable to other allosteric protein modules, providing a cost effective-strategy for the custom design of FRET sensors.

]]> Klein, Florencia]]> Sardi, Florencia]]> Machado, Matías R.]]> Ortega, Claudia]]> Comini, Marcelo A.]]> Pantano, Sergio]]> Frontiers]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.3389/fmolb.2021.629773]]> Contribution of anecic and epigeic earthworms to biological control of Fusarium graminearum in wheat straw]]> Earthworms have proved to contribute to plant health indirectly, by improvement of soil physical and chemical properties, as well as directly, through suppression of plant pathogens. Fusarium Head Blight, caused primarily by Fusarium graminearum, is one of the most important cereal diseases, with severe detrimental effects on yield and grain quality worldwide, and significant effect on grain safety due to the accumulation of mycotoxins produced by the fungus. Earthworms could reduce the residue-borne inoculum density of F. graminearum through different mechanisms, including direct competition by residue consumption, growth inhibition caused by the earthworm coelomic fluid, mechanical disruption of fungal hyphae, and burying crop residue which reduce the
chances for the fungus to release spores for head infection. Thus, the aim of this study was to assess the effects of anecic and epigeic earthworms on wheat straw and on F. graminearum inoculum. For this, PVC cylinders (microcosms 14.5 cm diameter, 30 cm height) were filled with moist soil mix, wheat straw was evenly distributed on the soil surface (inoculated with Fusarium, soil microorganisms or sterile) and three earthworm species (Aporrectodea longa, Lumbricus rubellus or Lumbricus terrestris) were tested. Since the response of earthworms may
change according to conditions of soil moisture and food availability, two different experiments were arranged. The first Experiment represented a sub-optimal situation for earthworms regarding soil moisture and feeding conditions, which was obtained by scarce watering of the microcosms with a consequent decrease in soil water content during incubation, and by not adding cow manure as supplementary food source. In the second experiment, soil was enriched with cow manure as feed for the earthworms and soil moisture was maintained
above 25%. Results from qPCR analysis revealed that F. graminearum inoculum on straw was reduced to undetectable
amounts by L. rubellus and A. longa when the feeding conditions were limited, while no significant differences compared with the control without earthworms were found when earthworms had high food availability (α = 0.05). Straw coverage on soil surface was reduced by L. rubellus (epigeic) in both experiments (p< 0.0001), while A. longa (anecic) just did so under optimal conditions. L. terrestris (anecic), only tested under optimal conditions, reduced soil cover significantly more than the other two species (p < 0.0001). Negative effect
of this fungus was not observed on the studied earthworms. Both anecic and epigeic earthworms tested showed potential to contribute to biological control of F. graminearum in wheat straw. This control may occur by reducing straw on surface, reducing the pathogen inoculum on straw, or both, depending on the environmental conditions and their response according to their ecological group and species. The possibilities to optimize earthworm capacity for biological control of fungal diseases in practical agriculture, under changing weather and organic matter supply conditions, are discussed.

]]> Jorge Escudero, Gabriela]]> Pérez, Carlos A.]]> Friberg, Hanna]]> Söderlund, Sara]]> Vero, Silvana]]> Garmendia, Gabriela]]> Lagerlöf, Jan]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1016/j.apsoil.2021.103997]]> References to home-made and natural foods on the labels of ultra-processed products increase healthfulness perception and purchase intention: insights for policy making ]]> As nutritional recommendations change, new marketing strategies are expected to appear on food labels to convey the idea that products are healthful and aligned with the recommendations. In this context, the objective of the study was to evaluate the effect of references to ‘home-made’, images of natural foods and nutritional warnings on consumers’ healthfulness perception and purchase intention of labels of ultra-processed products with excessive content of nutrients associated with non-communicable diseases (NCDs). The study was conducted with 790 Uruguayan Facebook users (65% female, 18–89 years old), diverse in terms of educational and
socio-economic levels. A choice experiment involving labels of four categories of ultra-processed products (burgers, cookies, instant soup and instant vanilla milk custard) was implemented. For each product category, eight sets of two labels differing in the presence of references to home-made, images of natural foods and nutritional warnings were designed. Half of the participants were asked to indicate the healthier product,
whereas the other half were asked to indicate the product they would purchase. Data were analyzed using mixed logit models. The inclusion of references to home-made and images of natural foods on the labels had a positive effect on purchase intention and tended to create the belief that products were healthful. Nutritional warnings decreased healthfulness perception and discouraged participants’ choice. In three of the product categories images of natural foods created a health halo that reduced the effect of nutritional warnings on product choice. Results from the present work suggest the need to develop stricter labelling regulations for ultra-processed
products with high content of nutrients associated with NCDs.

]]> Devia, Gabriela]]> Forli, Stefani]]> Vidal, Leticia]]> Curutchet, María Rosa]]> Ares, Gastón]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1016/j.foodqual.2020.104110]]> The impact of Hanseniaspora vineae fermentation and ageing on lees on the terpenic aromatic profile of white wines of the albillo variety]]> Hanseniaspora vineae is a non-Saccharomyces yeast that has a powerful impact on the sensory profile of wines. Its effect on the aromatic profile of non-aromatic grape varieties, such as Albillo Mayor (Vitis vinifera, L.), during vinification is a useful biotechnology to improve sensory complexity. Fermentation in steel barrels using Hanseniaspora vineae and sequential inoculation with Saccharomyces cerevisiae have been used to study the formation of terpenes and cell lysis in the production of Albillo white wines. The GC-MS analysis profile shows a significant effect of H. vineae fermentation on the contents of terpenes (3), mainly in linalool (>3), -citronellol (>4), geraniol (>2) and-terpineol (2). The contents of several polyoxygenated terpenes and some volatile phenols with a spicy aroma were increased during fermentation. In summary, Hanseniaspora vineae releases a large number of cell wall polysaccharides during fermentation that affect wine palatability and structure.
Hanseniaspora vineae is a powerful bio-tool to enhance the fruitiness, floral notes and freshness in non-aromatic white varieties.

]]> Fresno, Juan Manuel del]]> Escott, Carlos]]> Loira, Iris]]> Carrau, Francisco]]> Cuerda, Rafael]]> Schneider, Rémi]]> Bañuelos, María Antonia]]> González, Carmen]]> Suárez Lepe, José Antonio]]> Morata, Antonio]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.3390/ijms22042195]]> Influence of beta glucosidases from native yeast on the aroma of Muscat and Tannat wines]]> It is now well established that β-glucosidases (BGLs) from non-Saccharomyces yeasts are key enzymes that hydrolyze grape-derived aroma precursors enhancing the flavour of wines. This work reports on the specificity for wine glycosides and the impact on wine aroma, of three native yeast β-glucosidases. Volatile compounds were analyzed by gas-chromatography and mass spectroscopy (GC–MS) and wine aroma was studied by sensory analysis. Issatchenkia terricola β-glucosidase stood out from the other β-glucosidases studied. The I. terricola BGL
showed remarkable specificity for norisoprenoid aglycones such as: 3-oxo-7, 8-dihydro-alpha-ionol, 3-oxo- α-ionol, vomifoliol. This different specificity was perceived in the sensory tests. The judges described pleasant fruity, sweet, honey and raisin notes in both Tannat and Muscat wines treated with I. terricola BGL. These results are particularly remarkable for Tannat wines, since there are few reports concerning the application of β-glucosidases to enhance its aroma of Tannat, and none with BGLs from native yeasts.

]]> Ovalle, Stefani de]]> Brena, Beatriz]]> González Pombo, Paula]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> https://doi.org/10.1016/j.foodchem.2020.128899]]> 99mTc Stearyl 6-(benzylidenehydrazinyl) nicotinamide Liposomes as Tumor Permeability Evaluation Trace]]> Nanomedicine is a highly demanded discipline. Liposomes have seen an increased attention due to their physicochemical properties that allow them to act as nanocarriers of drugs and also of radioisotopes that can be used to diagnose and treat cancer. In order to obtain a novel permeability cancer imaging agent based on 99mTc-labeled liposomes, we describe microwave-assisted synthesis of stearyl 6 (benzylidenehydrazinyl) nicotinamide lipid, which was included in two formulations: nanometric hydrazinonicotinic acid (HYNIC) liposome and its PEGylated coated analogue, HYNIC-PEG liposome. Radiolabeling with 99mTc via stearyl 6-(benzylidenehydrazinyl) nicotinamide was found to
be easy, reproducible, and stable, revealing high radiochemical purity (94 ± 1.7%) for both liposomal formulations. Biodistribution at 4 h and 24 h and scintigraphic images at 4 h were performed in normal and melanoma-bearing C57BL/6 mice. Biodistribution studies at 4 h showed tumor uptake of 99mTc-HYNIC liposome and 99mTc-HYNIC-PEG liposome (1.1 ±
0.6 and 2.5 ± 0.4, respectively) and also at 24 h p.i. (1.8 ± 0.5 and 3.0 ± 1.1, respectively). Scintigraphic images showed appreciable tumor uptake in melanoma tumor–bearing mice
with both liposomal formulations. Our results show that 99mTc stearyl 6-(benzylidenehydrazinyl) nicotinamide liposomes can be used as diagnostic noninvasive in vivo tumor-targeting agents capable of evaluating tumor permeability and development who can be used in personalized chemotherapy planning.

]]> Cabrera, Mirel]]> Lecot, Nicole]]> Fernández Silveira, Marcelo]]> Gambini, J. P.]]> Porcal, Williams]]> Cabral, Pablo]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1208/s12249-021-01984-1]]> Optimized preparation of washcoated clay honeycomb monoliths as support of manganese catalysts for acetone total combustion]]> Clay honeycomb monoliths (MC) washcoated by pillared clay (PILC) obtained from the same starting material were used as support of manganese catalysts for the complete oxidation of acetone. Using the same clay for extrusion and pillaring processes intended to optimize the catalyst preparation respect to previous similar studies through raw materials saving, with subsequent local clay valorization, and washcoat adherence enhancement due to higher chemical affinity with the monolith substrate. To evaluate the influence of the PILC presence on the
catalytic behaviour, two other non-coated monolith catalysts were prepared as references, having different loads of manganese as function of the impregnation cycles number applied, either one (Mn/MC) or two (Mn2/MC). All the catalysts were characterized by TGA, XRD, XRF, N2 physisorption and SEM. The catalytic tests used acetone as a VOC model molecule. The results indicated that the PILC coating (Mn/PILC/MC) led to a higher percentage of Mn2O3 (7 wt%) compared to Mn/MC (1 wt%). Moreover, the PILC-washcoated sample exhibited better performance in the acetone oxidation than Mn2/MC regardless this sample had higher Mn2O3 loading (11 wt%). This effect was related to the active phase crystal characteristics as well as to the textural properties induced by
the PILC washcoating.

]]> De los Santos, Carolina]]> Vidal, Hilario]]> Gatica, Jose Manuel]]> Yeste, M. Pilar]]> Cifredo, Gustavo]]> Castiglioni, Jorge.]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1016/j.micromeso.2020.110651]]> A novel nitroalkene vitamin E analogue inhibits the NLRP3 inflammasome and protects against inflammation and glucose intolerance triggered by obesity]]> Chronic metabolic diseases, like obesity, type II diabetes and atherosclerosis often involve a low-grade and sterile systemic inflammatory state, in which activation of the pro-inflammatory transcription factor NF-kB and the NLRP3 inflammasome play a major role. It is well established that genetic inhibition of the NLRP3 inflammasome ameliorates acute and chronic inflammation. Indeed, accumulating experimental evidences in murine models and also in humans suggest that inhibition of the NLRP3 inflammasome might be a suitable approach to tackle the deleterious effects of chronic metabolic diseases. In this work, we explored our previously synthesized nitroalkene-Trolox™ derivative named NATx0, as a non-conventional anti-inflammatory strategy to treat chronic inflammatory diseases, such as obesity-induced glucose intolerance. We found that NATx0 inhibited NF-kB nuclear translocation and pro-inflammatory gene expression in macrophages in vitro. In addition, treatment with NATx0 prevented NLRP3 inflammasome activation after LPS/ATP stimulation in macrophages in vitro. When tested acutely in vivo, NATx0 inhibited neutrophil recruitment in zebrafish larvae, and also diminished IL- 1β production after LPS challenge in mice. Finally, when NATx0 was administered chronically to diet-induced obese mice, it decreased muscle tissue inflammation and glucose intolerance, leading to improved glucose homeostasis. In conclusion, we propose that this novel nitroalkene-Trolox derivative is a suitable tool to tackle acute and chronic inflammation in vitro and in vivo mainly due to inhibition of NF-kB/NLRP3 activation.

]]> Dapueto, Rosina]]> Galliussi, Germán]]> Kamaid, Andrés]]> Bresque, Mariana]]> Batthyány, Carlos]]> López, Gloria V.]]> Escande, Carlos]]> Rodríguez Duarte, Jorge.
]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1016/j.redox.2020.101833]]> Enantioselective Synthesis of a New Non-Natural Gabosine]]> The preparation of a new non-natural gabosine is reported, in which the chirality is transferred from the toluene’s biotransformed metabolite (1R,2S)-3-methylcyclohexa-3.5-diene-1,2- diol. Further chemical transformations to introduce additional functionality and chirality to the molecule were also accomplished.

]]> Colobbio, Maximiliano]]> Pandolfi, Enrique]]> Schapiro, Valeria]]> Información sobre Derechos de Autor

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]]> 10.3390/molecules26051423 ]]> Production of cadmium sulfde quantum dots by the lithobiontic Antarctic strain Pedobacter sp. UYP1 and their application as photosensitizer in solar cells]]> Background: Microbes are present in almost every environment on Earth, even in those with extreme environmental conditions such as Antarctica, where rocks may represent the main refuge for life. Lithobiontic communities are composed of microorganisms capable of colonizing rocks and, as it is a not so well studied bacterial community, they may represent a very interesting source of diversity and functional traits with potential for biotechnological applications. In this work we analyzed the ability of Antarctic lithobiontic bacterium to synthesize cadmium sulfde quantum dots (CdS QDs) and their potential application in solar cells.
Results: A basaltic andesite rock sample was collected from Fildes Peninsula, King George Island, Antarctica, and
processed in order to isolate lithobiontic bacterial strains. Out of the 11 selected isolates, strain UYP1, identifed as Pedobacter, was chosen for further characterization and analysis due to its high cadmium tolerance. A protocol for the biosynthesis of CdS QDs was developed and optimized for this strain. After 20 and 80 min of synthesis, yellow-green and orange-red fuorescent emissions were observed under UV light, respectively. QDs were characterized through spectroscopic techniques, dynamic light scattering analysis, high-resolution transmission electron microscopy and energy dispersive x-ray spectroscopy. Nanostructures of 3.07 nm, composed of 51.1% cadmium and 48.9% sulfde were obtained and further used as photosensitizer material in solar cells. These solar cells were able to conduct electrons and displayed an open circuit voltage of 162 mV, a short circuit current density of 0.0110 mA cm−2, and had an efciency of conversion up to 0.0016%, which is comparable with data previously reported for solar cells sensitized with biologically produced quantum dots.

Conclusions: We report a cheap, rapid and eco-friendly protocol for the production of CdS QDs by an Antarctic lithobiontic bacterium, Pedobacter, a genus that was not previously reported as a quantum dot producer. The application of the biosynthesized QDs as sensitizer material in solar cells was validated.

]]> Carrasco, V.]]> Amarelle, V.]]> Lagos Moraga, S.]]> Quezada, C. P.]]> Espinoza González, R.]]> Faccio, Ricardo.]]> Fabiano, E.]]> Pérez Donoso, J. M.
]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1186/s12934-021-01531-4]]> An Efficient Synthesis of Optically Active [4-13C] Labelled Quorum Sensing Signal Autoinducer-2 ]]> A new synthetic route for the quorum sensing signal Autoinducer-2 (AI-2) is described and used for the preparation of [4-13C]-AI-2 starting from [1-13C]-bromoacetic acid. The key step in this process was the enantioselective reduction of an intermediate ketone. This synthesis provides, selectively, both enantiomers of the labelled or unlabelled parent compound, (R) or (S)-4,5-dihydroxypentane-2,3-dione (DPD) and was used for an improved synthesis of [1-13C]-AI-2.

]]> Ascenso, Osvaldo S.]]> Carrau, Gonzalo]]> Xavier, Karina B.]]> Ventura , M. Rita]]> Maycock, Christopher D.]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.3390/molecules26020369]]> Redox biocatalysis : fundamentals and applications]]> Gamenara, Daniela]]> Seoane, Gustavo A.]]> Saenz Méndez, Patricia]]> Domínguez de Maía, Pablo]]> Información sobre Derechos de Autor

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]]> Nanotecnología: ejemplos y aplicaciones en la utilización de energía solar ]]> En este capítulo presentaremos de manera general aplicaciones
que la nanotecnología puede ofrecer, tanto desde el punto de vista de procesos como también de los diversos productos basados en esta nueva tecnología que actualmente se comercializan. Posteriormente discutiremos con mayor profundidad las oportunidades que ofrece el uso de la energía
solar, en particular las ventajas del uso de la nanotecnología. De esta forma mostraremos cómo esta nueva revolución tecnológica permite ir más allá de lo que se había pensado: generar nuevos productos, presentar variantes en
productos ya existentes, generar nuevos procesos, hacer viables procesos que antes no lo eran, y todo esto cuidando el ambiente.
La nanotecnología (NT) consiste en la creación de materiales
funcionales, dispositivos y sistemas a través de la manipulación de la materia a escala nanométrica. Un nanomaterial para ser catalogado como tal debe poseer en al menos una de sus dimensiones una longitud inferior a los 100 nanómetros (nm). Pero la nanotecnología también incluye la explotación de
las propiedades y los fenómenos ocurrentes a esa escala (Los Alamos National Laboratory, s/f), ya que son notoriamente diferentes a los esperados para los materiales en su forma convencional.

]]> Faccio, Ricardo]]> Las nanotecnologías en Uruguay / Adriana Chiancone y Guillermo Foladori, coords. -- Montevideo : Espacio interdisciplinario, 2013. -- p. 55-63]]> UR. Espacio Interdisciplinario]]> Información sobre Derechos de Autor

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]]> ISBN 978-9974-0-0961-5]]> Nanomateriales con potenciales aplicaciones tecnolgógicas desarrollados en el Centro NanoMat, Polo Tecnológico de Pando, Facultad de Química.]]> En este artículo se presentará un resumen de algunas de las líneas de investigación que se vienen desarrollando en el Centro NanoMat, y que son consideradas como estratégicas tanto desde el punto de vista de su relevancia y potencialidades como porque pueden ser llevadas adelante exitosamente por el Centro con sus actuales capacidades. Sin embargo, cabe señalar que dichas capacidades están en permanente crecimiento desde su instalación, en el 2008, lo que permite el abordaje de nuevos emprendimientos.

]]> Pardo, Helena]]> Las nanotecnologías en Uruguay / Adriana Chiancone y Guillermo Foladori, coords. -- Montevideo : Espacio interdisciplinario, 2013. -- p. 43-53]]> UR. Espacio Interdisciplinario]]> Información sobre Derechos de Autor

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]]> ISBN 978-9974-0-0961-5]]> Nanotecnología y Uruguay]]> La nanotecnología (NT) es una rama de la tecnología en la que se realizan procesos con la materia a una escala nanométrica. Un nanometro o nanómetro es la millonésima parte de un milímetro. Dicho de esta manera, es muy difícil aquilatar la dimensión de referencia, la que da origen al mismo nombre de esta tecnología.
Se podría agregar que casi se ingresa en la zona del tamaño de los propios átomos, pero si bien esta aclaración suena a algo muy pequeño, probablemente no sea suficiente para darse cuenta de la real magnitud de lo que hablamos. Sin embargo, todos tenemos una noción de la pequeñez de las células, que se definen como la menor unidad de los organismos vivos y
que todos entendemos como algo muy pequeño. A las células las entendemos como parte de nosotros, pero también hablamos de gérmenes y contagios o infecciones, técnicamente provocadas por bacterias, que son en sí mismas seres vivos unicelulares. Ahora bien, un nanometro es entre mil y diez mil
veces más pequeño que una célula promedio, lo que nos dimensiona el tema más claramente.
Cuando hablamos de NT estamos incluyendo todas las tecnologías que tratan de procesos o productos en esta escala, ya sea productos finales o productos intermedios que se usen como precursores, o incluso sustancias que a esa escala favorezcan que tenga lugar una reacción que de otra manera
no ocurriría.

]]> Mombrú, Alvaro]]> UR. Espacio Interdisciplinario]]> 2013

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]]> Ectopic spacer acquisition in Streptococcus thermophilus CRISPR3 array]]> Streptococcus thermophilus relies heavily on two type II-A CRISPR-Cas systems, CRISPR1 and CRISPR3, to resist siphophage infections. One hallmark of these systems is the integration of a new spacer at the 50 end of the CRISPR arrays following phage infection. However, we have previously shown that ectopic acquisition of spacers can occur within the CRISPR1 array. Here, we present evidence of the acquisition of new spacers within the array of CRISPR3 of S. thermophilus. The
analysis of randomly selected bacteriophage-insensitive mutants of the strain Uy01 obtained after phage infection, as well as the comparison with other S. thermophilus strains with similar CRISPR3 content, showed that a specific spacer within the array could be responsible for misguiding the adaptation complex. These results also indicate that while the vast majority of new spacers are added at the 50 end of the CRISPR array, ectopic spacer acquisition is a common feature of both
CRISPR1 and CRISPR3 systems in S. thermophilus, and it can still provide phage resistance. Ectopic spacer acquisition also appears to have occurred naturally in some strains of Streptococcus pyogenes, suggesting that it is a general phenomenon, at least in type II-A systems.

]]> Achigar, Rodrigo]]> Scarrone, Martina]]> Rousseau, Geneviève M.]]> Philippe, Cécile]]> Machado, Felipe]]> Duvós, Valentina]]> Campot, María Pía]]> Dion, Moïra B.]]> Shao, Yuyu]]> Pianzzola, María Julia]]> Moineau, Sylvain]]> MDPI ]]> Información sobre Derechos de Autor

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]]> Artículo

]]> https://doi.org/10.3390/microorganisms9030512]]> Global analysis of the apple fruit microbiome: are all apples the same? ]]> We present the first worldwide study on the apple (Malus × domestica) fruit microbiome that examines questions regarding the composition and the assembly of microbial communities on and in apple fruit. Results revealed that the composition and
structure of the fungal and bacterial communities associated with apple fruit vary and are highly dependent on geographical location. The study also confirmed that the spatial variation in the fungal and bacterial composition of different fruit tissues exists at a global level. Fungal diversity varied significantly in fruit harvested in different geographical locations and suggests a potential link between location and the type and rate of postharvest diseases that develop in each country. The global core microbiome of apple fruit was represented by several beneficial microbial taxa and accounted for a large fraction of the fruit microbial community. The study provides foundational information about the apple fruit microbiome that can be utilized for the development of novel approaches for the management of fruit quality and safety, as well as for reducing
losses due to the establishment and proliferation of postharvest pathogens. It also lays the groundwork for studying the complex microbial interactions that occur on apple fruit surfaces.

]]> Abdelfattah, Ahmed]]> Freilich, Shiri]]> Bartuv, Rotem]]> Zhimo, V. Yeka]]> Kumar, Ajay]]> Biasi, Antonio]]> Salim, Shoshana]]> Feygenberg, Oleg]]> Burchard, Erik]]> Dardick, Christopher]]> Liu, Jia]]> Khan, Awais]]> Ellouze, Walid]]> Ali, Shawkat]]> Spadaro, Davide]]> Torres, Rosario]]> Teixido, Neus]]> Ozkaya, Okan]]> Buehlmann, Andreas]]> Vero, Silvana]]> Mondino, Pedro]]> Berg, Gabriele]]> Wisniewski, Michael]]> Droby, Samir]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> https://doi.org/10.1111/1462-2920.15469]]> How do consumers use emoji in a food-related context? Insights for the design and interpretation of emoji questionnaires]]> The present research contributed to the growing body of research regarding the use of emoji in sensory and consumer research by filling an acknowledged knowledge gap. Its specific aim was to explore the meaning of emoji in a food-related context. The empirical approach involved 357 consumers who were tasked with writing a social media post or text message relating to a situation involving eating/drinking which was to be centered around a specific emoji. A total of 21 facial emoji were
included in the research. As expected, emoji were used to convey different meanings, including degree of liking/disliking, emotional associations, gestures and expressions.
The emoji were also used as part of messages describing negative and positive disconfirmation of expectations, which points to the importance of measuring expectations in a food-related context. Two emoji were identified as unsuitable for future research due to their nonconsensual meaning ( and ).

]]> Ares, Gastón]]> Vidal, Leticia]]> Jaeger, Sara R.
]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Sugar reduction in products targeted at children: Why are we not there yet? ]]> Sugar intake among children has raised concern worldwide as it exceeds nutritional recommendations.
Sugar contributes to the daily energy intake, without providing additional nutritional value and is associated with several negative health outcomes. Sugars added to foods during industrial processing have been identified as the main source of sugar in children's diets. The present work critically discusses the role of the food industry in contributing to children's excessive sugar intake worldwide, and the strategies that have
been encouraged or implemented to reduce the sugar content in products targeted at children. The risk of the current sugar reduction practices in products targeted at children is discussed based on recent scientific evidence. Children's heightened preference for sweetness may not justify the availability of highly sugary products for children. Although research suggests that children readily accept less sweet products, there is still
some wariness in the food industry to reduce sweetness intensity. This has strengthened the use of non-nutritive sweeteners (NNS), focusing on maintaining the sweetness level.
However, emerging evidence suggests that this may not be the best approach.

]]> Velázquez, Ana Laura]]> Vidal, Leticia]]> Varela, Paula]]> Ares, Gastón]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Lithraea molleoides (Vell.) Engler]]> Lithraea molleoides (Anacardiaceae) is a tree with a broad area of distribution in the southern region of South America, including Southern Brazil, Southern, and Eastern Bolivia, Southern Paraguay, Northern, and Central Argentina, and
reaches its southernmost distribution limit in Northern and Northeastern Uruguay. It presents considerable morphological variability. It is used as medicinal plant and as a sweetener in traditional infusions. There have been reports on its antibacterial, antiviral, antioxidant, anti-inflammatory and antinociceptive activities. It is traditionally known that it may induce allergic contact dermatitis in sensitive persons by
direct and/or airborne contact, i.e. when used as firewood. Many of its biological activities have been associated with the reported presence of 5-alkyl/alkenyl resorcinols. Its allergenic activity has been ascribed to 3-alkyl/alkenyl catechols, as it
is well known for other species of the Anacardiaceae family. The wide distribution of the species, its morphological plasticity, the traditional medicinal uses, and toxic properties indicate the necessity of a more extensive and systematic study of the
species to assess its medicinal and biological potential.

]]> López, Paula Gladys]]> Basile, Patricia]]> Wallace, Federico]]> Olivaro, Cristina]]> Minteguiaga, Manuel]]> Ferreira, Fernando]]> Información sobre Derechos de Autor

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