Ilex paraguariensis St. Hil.]]> Ilex paraguariensis St. Hil., from the family of holy plants, Aquifoliaceae, is a native South American tree that can reach 18 m in height and is used to produce “yerba mate”. It is found primarily in the southern regions of South America, namely, Brazil (Mato Grosso do Sul, Minas Gerais, Parana, Rio Grande do Sul, Rio de Janeiro, Santa Catarina, Sao Paulo), Argentina (Corrientes, Misiones), Paraguay (Alto Paraná, Amambay, Caaguazú, Canendiyú, Central, Guaira, Itapuá, Misiones,
San Pedro), and Uruguay. “Yerba mate” tea, an infusion made from the leaves of this tree, is a widely consumed nonalcoholic beverage in South America which is gaining rapid introduction into the world market, either as tea itself or as ingredient
in formulated foods or dietary supplements. The indigenous people have used it for centuries as a social and medicinal beverage. The container in which it is generally served is a gourd made from the dry and hollowed fruit of Lagenaria vulgaris Ser. (Cucurbitaceae), whose dimensions vary according the region and uses. It is called “mate” in the region. “Mate” has a very important social role. The act of offering and sharing it has connotations like those of the tea ceremony for some Oriental cultures.

]]> Bracesco , Nelson]]> Minteguiaga, Manuel]]> Vázquez, Álvaro]]> Cesio, Verónica]]> Heinzen, Horacio]]> Ferreira, Fernando]]> Dellacassa Beltrame, Eduardo Santiago]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> Lorenzo Otero, Daniel Alberto.]]> Paz, Daniel]]> Davies, Philip]]> Villamil, José]]> Minteguiaga, Manuel]]> Dellacassa Beltrame, Eduardo Santiago]]> Información sobre Derechos de Autor

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ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

]]> https://doi.org/10.1007/978-3-030-62818-5_15]]> Cissampelos pareira L. ]]> Cissampelos pareiraL. (Menispermaceae) is a widely used medicinal plant, also used to treat bites of venomous animals, particularly snakes. Ophidian accidents are a serious public health problem in Argentina where the Bothrops genus of snakes is responsible for 97% of these accidents, peculiarly B. diporus (“yarará chica”) responsible for 80% of them. In the northeast of the country (Corrientes Province), C. pareira is commonly used against the venom of B. diporus; its use is described in almost all-ethnobotanical literature of the countries where the plant grows. In vitro and in vivo antivenom activities of C. pareira extracts from plants collected at two different phytogeographic regions of Corrientes (Argentina) were evaluated against B. diporus venom. The seasonal influence on the chemical composition of extracts was also studied to determine the associated range of variability and its influence on the antivenom activity. Besides, an evaluation of the chemical composition of the volatile extract was performed to assess its chemical stability. These results support the ethnopharmacological use of this species. Moreover, the presented data demonstrate that certain flavonoids may mitigate some of the venom-induced local tissue damages.

]]> Dellacassa Beltrame, Eduardo Santiago]]> Información sobre Derechos de Autor

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]]> Ibicella lutea (Lindl.) van Eselt.]]> MARTYNIACEAE]]> IBICELLA LUTEA]]> Ibicella lutea (Lindl.) van Eselt. is the main species of the genus Ibicella. It belongs to the small Martyniaceae family and it is used, along with less common species of Ibicella such as I. parodii and I. nelsoniana, in remedies of popular medicine. The phytochemical analysis of the plants showed the presence of several fatty
acid glycosides with antibacterial activity against Staphylococcus aureus, as well as several strains of uropathogenic Proteus mirabilis. These facts help explain the widespread ethnopharmacological use of the plant.

]]> Vázquez, Álvaro]]>

Medicinal and Aromatic Plants of South America Vol. 2 : Argentina, Chile and Uruguay / Ákos Máthé and Arnaldo Bandoni, eds. -- [s.l.] : Springer, 2021. -- p. 271-277

]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1007/978-3-030-62818-5_21]]> Nectandra angustifolia (Schrad.) Nees & Mart.

]]> Medicinal plants encompass a rich source of active compounds that can neutralize snake venoms or toxins. Nectandra angustifolia (Schrad.) Nees & Mart. (Lauraceae), commonly known as “laurel amarillo”, “aju’yhû”, “laurel-hu”, “laurel
del río”, is used in traditional medicine as a digestive, for the treatment of rheumatism, arthritis, pain, and as antivenom. Snakebite envenomation was included in category A of neglected tropical diseases (NTD), in 2017, as they affect millions of people in the world (Chippaux, J Venom Anim Toxins 23:38–39: 2017). In Argentina, the snake genus Bothrops is responsible for the greatest number of accidents. Presently, the application of serum antivenom is the only specific treatment. This treatment does not protect over local effects, which are the main cause of morbidity. In the search for effective antivenoms, extracts of different polarity obtained from Nectandra angustifolia were evaluated both, in vitro and in vivo, against the venom of B. diporus "yarará chica". The effect of seasonal variability in this activity was also studied, to optimize both, the harvest period and the most appropriate type of extract. By successive bio-guided fractionations of the ethanol extract of N. angustifolia a fraction enriched in flavonoids responsible for the alexiteric activity of the species has been isolated. The phytochemical study of the volatile fraction of N. angustifolia and N. megapotamica allowed for the phytochemical characterization of farther interesting compounds.

]]> Torres, Ana María ]]> Camargo, Francisco José ]]> Ricciardi Verrastro, Bárbara Verónica ]]> Dellacassa Beltrame, Eduardo Santiago]]> Ricciardi, Gabriela Ana Leticia

]]> Medicinal and Aromatic Plants of South America Vol. 2 : Argentina, Chile and Uruguay / Ákos Máthé and Arnaldo Bandoni, eds. -- [s.l.] : Springer, 2021. -- p. 379-387]]> Información sobre Derechos de Autor

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]]> Passiflora caerulea L.]]> Passiflora caerulea L. (Passifloraceae) (“mburucuyá”, “maracujá-laranja”), a widely distributed species in South America, has been traditionally considered as a medicinal, ornamental, and edible plant. Its fruits, besides being well
appreciated for their flavor and sweetness, have valuable nutritional properties. The ethnopharmacological use as anxiolytic, antistress and sedative of the non-reproductive
aerial parts (leaves and tendrils) from P. caerulea is the main reason for its commercialization as an infusion herb. In general, its preparations are rich in flavonoids (chrysin and C-glycosides such as vitexin, isovitexin, orientin, isoorientin, and vicenin-2, among others), which were confirmed as responsible for the anxiolytic effect. The Argentine Pharmacopeia includes a monograph on P. caerulea, while other Pharmacopeias (Brazilian, European, and British) consider it as a potential
adulterant of P. incarnata and P. edulis. Harmala alkaloids from P. caerulea might also contribute to the anxiolytic effect, but no preclinical assays have been conducted to confirm it. Pharmacological research confirmed other bioactivities associated with the aerial parts of P. caerulea, such as antimicrobial, gastroprotective, analgesic, and anticonvulsant. Even though, no systematic toxicological assessments have been conducted in order to evaluate the safety in the consumption, especially regarding the presence of cyanogenic glycosides both in the vegetal drug and the unripe fruits.

]]> Minteguiaga, Manuel ]]> Wallace, Federico]]> Basile, Patricia]]> Ferreira, Fernando

]]> Olivaro, Cristina]]> Dellacassa, Eduardo ]]> Medicinal and Aromatic Plants of South America Vol. 2 : Argentina, Chile and Uruguay / Ákos Máthé and Arnaldo Bandoni, eds. -- [s.l.] : Springer, 2021. -- p. 411-427]]> Información sobre Derechos de Autor

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]]> Quillaja brasiliensis (A. St.-Hil. & Tul.) Mart.]]> Quillaja brasiliensis (A. St.-Hil. & Tul.) Mart. is currently one of the only two species of the only genus in the Quillajaceae D. Don (Dicotyledoneae) family. Recently, remarkable developments have been achieved regarding its domestication,
propagation, and biochemical study. The first results on the structural study of the complex mixture of saponins produced by different organs of the tree have been published. Analogously to the related species Q. saponaria Molina, its saponins, either alone or in colloidal formulations, have been proved to be very effective as adjuvants in experimental vaccines, being able to elicit an early humoral and cellular response against the co-administered antigens. In consideration of the growing knowledge on Q. brasiliensis, this species constitutes a very promising botanical source for the]]> Ferreira Chiesa, Fernando
]]> Bennadji, Zohra ]]> Wallace, Federico ]]> Minteguiaga, Manuel ]]> Basile, Patricia ]]> Fleck, Juliane Deise ]]> Verza, Simone Gasparín]]> Olivaro, Cristina

]]> Medicinal and Aromatic Plants of South America Vol. 2 : Argentina, Chile and Uruguay / Ákos Máthé and Arnaldo Bandoni, eds. -- [s.l.] : Springer, 2021. -- p. 447-459]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1007/978-3-030-62818-5_35]]> Smilax campestris Griseb.]]> Smilax campestris Griseb. (Smilacaceae) (“zarzaparrilla”) is a medicinal plant species widely used by traditional communities in South America. In general, the plant parts used by popular medicine are the rhizophore (underground stem) and roots, in the form of infusions or decoctions. Their main indications are: antirheumatic, antisyphilitic, diuretic, diaphoretic, anti-inflammatory, antiseptic, and to treat skin conditions (i.e. psoriasis) and gout; among others. There is lacking scientific evidence regarding the phytochemistry of such aqueous extracts, as well as their biological and pharmacological properties. Both aerial and underground organs have been described to contain polyphenolic acids, flavonoids (aglycones, glycosides and proanthocyanidin oligomers/polymers) and saponins; but no systematic research on the isolation of other families of natural products has been conducted, yet. The production of secondary metabolites (in particular flavonoids) is highly dependent on both environmental conditions (solar radiation, herbivores, etc.) of habitats and the phenological stage of the plant itself. The scientific pharmacological in vitro tests performed on S. campestris extracts have confirmed their antioxidant and anti-inflammatory activities, as well as the lack of acute toxicity. However, they have failed to demonstrate diuretic effects in in vivo models; results on the antimicrobial potential are controversial. Further research is needed to evaluate the other ethnomedicinal properties attributed to the vegetal drug. This chapter presents a critical review of the special literature on S. campestris including the most updated information available in journals, books and the internet.]]> Minteguiaga, Manuel ]]> Ferreira, Fernando]]> Medicinal and Aromatic Plants of South America Vol. 2 : Argentina, Chile and Uruguay / Ákos Máthé and Arnaldo Bandoni, eds. -- [s.l.] : Springer, 2021. -- p. 493-508]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1007/978-3-030-62818-5_38]]> Do nutritional warnings encourage healthier choices on food ordering websites?: an exploratory experimental study in Uruguay]]> Objective: To evaluate if the inclusion of nutritional warnings in food ordering web-sites can discourage consumers from purchasing foods with excessive content of nutrients associated with non-communicable diseases (NCD).

Design: Participants were randomly assigned to one of the two experimental conditions: control (n 225) or nutritional warnings (n 222). Nutritional warnings corresponded to separate black octagonal signs containing the word ‘Excess’ followed by the corresponding nutrient: total fat, saturated fat, sugars and sodium.

Participants were asked to purchase a lunch for themselves using a simulated food ordering website.

Setting: Online study in Uruguay.

Participants: Convenience sample of 447 Uruguayan participants, recruited using social media.

Results: In the control condition, 76 % of the participants selected a dish or a beverage with excessive content of at least one nutrient in the simulated food ordering website. When nutritional warnings were included, this percentage significantly decreased to 62 % (P = 0·002). In addition, nutritional warnings caused a significant reduction in the percentage of participants who selected dishes with excessive content of total fat: 50 % v. 62 % (P = 0·012).

Conclusions: Results from the present work provide preliminary evidence that the inclusion of nutritional warnings in food ordering websites could discourage consumers from selecting dishes and beverages with excessive content of nutrients associated with NCD.

]]> Gugliucci, Vanessa]]> Machín, Leandro]]> Curutchet, María Rosa]]> Ares, Gastón.]]> Cambridge University Press]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> DOI: https://doi.org/10.1017/S1368980021001026]]> Parents’ mental associations with ultra-processed products for their infant children: insights to improve complementary feeding practices]]> Increasing consumption of ultra-processed products from early age has raised growing concerns. In this context, the aims of the present work were: i) to explore the automatic mental associations raised by packages of ultra-processed products among parents of children under 12 months of age, ii) to estimate the valence of the affective reactions elicited by ultra-processed products, and iii) to explore the influence of socio-demographic variables and feeding styles on the valence of parents’ associations with ultra-processed products. A total of 419 parents of children under 12 months of age were recruited at 20 health facilities that offer well-child services, selected using probability proportional to size sampling based on the number of births at each institution. Participants were presented with the labels of six ultra-processed products (corn snack, frankfurters, milk dessert, orange juice, wafer cookies, yogurt) and were asked to indicate the first words that came to their mind. The products raised mixed mental associations, which were mainly related to pleasure and health. Responses from the majority of participants (61–82%) evidenced negative associations with corn snacks, frankfurters and wafer cookies, whereas responses from 35 to 45% of the participants suggested a positive affective reaction towards orange juice, milk dessert and yogurt. Likelihood of having a positive affective reaction towards the evaluated ultra-processed products was higher for parents of 1 to 4 months infants, as well as those from low educational level and low socio-economic status. In addition, beliefs about three feeding practices (restriction on diet quality, indulgence in screen use and pressure for finishing) significantly influenced the valence of the associations. Results from the present work suggest the need to implement regulatory measures to reduce positive associations with ultra-processed products among parents and contribute to reduce their consumption among infants.

]]> Vidal, Leticia]]> Gerónimo, Brunet]]> Bove, Isabel]]> Girona, Alejandra]]> Fuletti, Darío]]> Ares, Gastón]]> Información sobre Derechos de Autor

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ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

]]> https://doi.org/10.1016/j.foodqual.2021.104335]]> How do consumer characteristics influence responses to nutritional warnings?]]> The aim of the present work was to explore the influence of consumer characteristics on purchase decisions when facing
products with nutritional warnings a few days after their implementation in Uruguay. A non-probabilistic sample of 917
participants was obtained using an advertisement on social media. Participants were asked if they had seen the warning
signs when making their food purchases and if they had seen the warnings on any product they intended to buy. Participants
who answered affirmately (n= 616) were asked about their purchase decision by answering the question “What have you
done with the product?” using the following response options: ‘I purchased it anyway’, ‘I purchased a similar product with
fewer excess signs’, ‘I purchased a similar product without excess signs’, ‘I didn’t purchase the product or any similar one’.
Univariate and multivariate multinomial logistic regression models were used to explore the influence of individual variables on participants’ likelihood of having taken different decisions when facing a product with warnings. Results showed that participants older than 55 years were more likely to react to the warnings by purchasing a similar product with fewer warnings or by not purchasing any product. Likelihood of modifying purchase decisions due to the inclusion of the warnings was associated with a frequent consumption of natural and minimally processed foods and a low consumption frequency of ultra-processed products. These results provide insights to target efforts to promote the use of nutritional warnings in decision making.

]]> Ares, Gastón]]> Antúnez, Lucía]]> Gugliucci, Vanessa]]> Curutchet, María Rosa]]> Galicia, Luis]]> Moratorio, Gimena]]> Giménez, Ana]]> Bove, Isabel]]> Información sobre Derechos de Autor

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]]> ISSN 0717-7518]]> 2-Mercaptomethyl-thiazolidines use conserved aromatic–S interactions to achieve broad-range inhibition of metallo-β-lactamases]]> Infections caused by multidrug resistant (MDR) bacteria are a major public health threat. Carbapenems are among the most potent antimicrobial agents that are commercially available to treat MDR bacteria. Bacterial production of carbapenem-hydrolysing metallo-β-lactamases (MBLs) challenges their safety and efficacy, with subclass B1 MBLs hydrolysing almost all β-lactam antibiotics. MBL inhibitors would fulfil an urgent clinical need by prolonging the lifetime of these life-saving drugs. Here we report the synthesis and activity of a series of 2-mercaptomethyl-thiazolidines (MMTZs), designed to replicate MBL interactions with reaction intermediates or hydrolysis products. MMTZs are potent competitive inhibitors of B1 MBLs in vitro (e.g., Ki = 0.44 μM vs. NDM-1). Crystal structures of MMTZ complexes reveal similar binding patterns to the most clinically important B1 MBLs (NDM-1, VIM-2 and IMP-1), contrasting with previously studied thiol-based MBL inhibitors, such as bisthiazolidines (BTZs) or captopril stereoisomers, which exhibit lower, more variable potencies and multiple binding modes. MMTZ binding involves thiol coordination to the Zn(II) site and extensive hydrophobic interactions, burying the inhibitor more deeply within the active site than D/L-captopril. Unexpectedly, MMTZ binding features a thioether–π interaction with a conserved active-site aromatic residue, consistent with their equipotent inhibition and similar binding to multiple MBLs. MMTZs penetrate multiple Enterobacterales, inhibit NDM-1 in situ, and restore carbapenem potency against clinical isolates expressing B1 MBLs. Based on their inhibitory profile and lack of eukaryotic cell toxicity, MMTZs represent a promising scaffold for MBL inhibitor development. These results also suggest sulphur–π interactions can be exploited for general ligand design in medicinal chemistry.]]> Rossi, María Agustina]]> Martínez, Verónica]]> Hinchlife, Philip]]> Mojica, María F.]]> Castillo, Valerie]]> Moreno, Diego M.]]> Smith, Ryan]]> Drusano, George L.]]> Banchio, Claudia]]> Bonomo, Robert A.]]> Spellberg, Brad]]> Spencer, James]]> Vila, Alejandro J.]]> Mahler, G.]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1039/D0SC05172A]]> Alcance y monitoreo de la Ley N° 19.140 en centros educativos de Montevideo.]]> Girona, Alejandra]]> Curuchet, María Rosa]]> Pereira, Pablo]]> Iragola, Valentina]]> Alcaire, Florencia]]> Magnani, Daiana]]> Barreto, Patricia]]> Petingi, Sofía]]> Allegue, Gimena]]> Conlazo, Gonzalo]]> Mosteiro, Camila]]> Ares, Gastón]]> Información sobre Derechos de Autor

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La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> L-Arginine metabolism during interaction of Trypanosoma Cruzi with host cells]]> Peluffo, G.]]> Piacenza, L.]]> Irigoin, F.]]> Alvarez, M.N.]]> Radi, R.]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

]]> Deterioro del aceite durante el almacenamiento de los higados de merluza en comparación con el del aceite extraido por ellos]]> Grompone, M.A.]]> Harispe, R.]]> Pagano, M.T.]]> Pinchak, Y.]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> Baccharis dracunculifolia DC.]]> BACCHARIS DRACUNCULIFOLIA]]> Baccharis dracunculifolia DC. (Asteraceae) is an aromatic plant species widely distributed across and along South American countries where it grows in different environments, even being considered, in some cases, as a hard to control weed. Its essential oil is highly appreciated in the fragrance/perfumery industry by its woody, foral, and green notes; mainly attributed to (E)-nerolidol and other sesquiterpene alcohols. Recent research has demonstrated a wide range of applications for the essential oil besides its aromatic properties. Although B. dracunculifolia cannot be considered as a medicinal plant for most of the indigenous communities in South America (except for Brazil), modern pharmacological research has demonstrated its high potential for the development of new therapeutic drugs, mainly with antiulcer, anti-infammatory, antiparasitic and anticancer effect. The special literature regarding B. dracunculifolia is profuse and thus, this chapter attempts to summarize the principal knowledge about this species.

]]> Minteguiaga, Manuel.]]> González, H. Andrés]]> Ferreira, Fernando.]]> Dellacassa, Eduardo.]]> Medicinal and Aromatic Plants of South America Vol. 2 : Argentina, Chile and Uruguay / Ákos Máthé and Arnaldo Bandoni, ed. -- [s.l.] : Springer, 2021. -- p. 85-105]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1007/978-3-030-62818-5_5]]> Immobilized peptide-N-glycosidase F onto magnetic nanoparticles: A biotechnological tool for protein deglycosylation under native conditions]]> The elucidation of glycans biological function is essential to
understand their role in biological processes, both normal and
pathological. Immobilized glycoenzymes are excellent tools for this purpose as they can selectively release glycans from glycoproteins without altering their backbone. They can be easily removed from the reaction mixture avoiding their
interference in subsequent experiments. Here, we describe the
immobilization of peptide-N-glycosidase F (PNGase F) onto
silica magnetic nanoparticles with immobilization yields of
86% and activity yields of 12%. Immobilized PNGase F showed
higher thermal stability than its soluble counterpart, and could
be reused for at least seven deglycosylation cycles. It was
efficient in the deglycosylation of several glycoproteins (ribonuclease B, bovine fetuin, and ovalbumin) and a protein lysate from the parasite Fasciola hepatica under nativeconditions, with similar performance to that of the solubleenzyme. Successful deglycosylation was evidenced by adecrease in specific lectin recognition of the glycoproteins (40%–80%). Moreover, deglycosylated F. hepatica lysate allowed us to confirm the role of parasite N-glycans in the inhibition of the lipopolysaccharide-induced maturation of dendritic cells. Immobilized PNGase F probed to be a robust biotechnological tool for deglycosylation of glycoproteins and complex biological samples under native conditions.

]]> Bidondo, Lucía.]]> Festari, Florencia.]]> Freire, Teresa.]]> Giacomini, Cecilia.]]> Información sobre Derechos de Autor

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https://doi.org/10.1002/bab.2099

]]> COVID-washing of ultra-processed products : the content of digital marketing on Facebook during the COVID-19 pandemic in Uruguay]]> Objective: To explore the use of references to the COVID-19 pandemic as part of the marketing strategies used on Facebook to promote ultra-processed products.

Design: A search for Facebook accounts of ultra-processed products was perFormed using a master list of products commercialised in two online supermarkets in Uruguay. For each of the identified Facebook accounts, all the content posted from the confirmation of the first cases of COVID-19 in Uruguay, on 14 March 2020, until 1 July 2020 was recorded. Posts including mentions to COVID-19, social distancing measures or their consequences were identified and analysed using content analysis.

Setting: Uruguay, Latin America.

Results: A total of 135 Facebook accounts were identified, which generated a total of 1749 posts related to ultra-processed products, from which 35 % included references to COVID-19. The majority of the posts included references to prevention measures. Approximately one-third of the posts included proposals of activities to do at home, most of which were linked to a healthy lifestyle. Tips for coping with quarantine and descriptions of the charitable work undertaken by brands were also identified.

Conclusions: Results from the present work provide evidence that industries of ultra-processed products have taken advantage of the COVID-19 pandemic to promote their products, create positive associations with the brands and improve their image as part of their digital marketing strategies.

]]> Antúnez, Lucía.]]> Alcaire, Florencia.]]> Brunet, Gerónimo]]> Bove, Isabel.]]> Ares, Gastón.]]> Información sobre Derechos de Autor

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]]> Jaeger, Sara R.]]> Vidal, Leticia.]]> Ares, Gastón.]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> Capítulo de Libro]]> eBook ISBN: 9780128231999]]> Biogenic silver nanoparticles as a strategy in the fight against multi-resistant salmonella enterica isolated from dairy calves.]]> Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise, the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. Salmonella enterica is an important pathogen that affects a wide range of animal species and humans, being Salmonella Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Salmonella Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which suggested that the nanoparticles produced damage in several bacterial cell structures.

]]> Estevez, María Belén.]]> Casaux, María Laura.]]> Fraga, Martín.]]> Faccio, Ricardo.]]> Alborés, Silvana.]]> Frontiers Media ]]> Información sobre Derechos de Autor

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]]> Cuña, A.]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

]]> 10.1109/NSSMIC.2002.1239263

]]> In vitro bioaccessibility of Cu and Zn in cooked beef cuts]]> Cu and Zn bioaccessibility was studied in two different beef cuts (rib plate and top sirloin), through the development of an in vitro assay. Samples were cooked at two degrees of doneness (medium and well-done) as usually consumed locally, to establish the contribution to the recommended daily allowance (RDA). Simulated gastrointestinal conditions were optimized and trueness of the method was evaluated by performing mass balances. Analytical determinations were performed by atomic absorption spectrometry and microwave-induced plasma optical emission spectrometry. No significant differences were found between the studied cuts nor between the degrees of doneness evaluated. Mean bioaccessible fraction (BF) of Zn was 83%, for both cuts and both degrees of doneness. In the case of Cu, rib plate presented a mean BF of 20%, while for top sirloin was of 38%. However, these differences found between cuts and degrees of doneness were not statistically significant. The contribution of Cu and Zn to the RDA for adults over 19 years was estimated, as an example, considering a 200 g portion. Results showed that the RDA of Zn would be completely covered, while the contribution to the RDA of Cu would be up to 33%.

]]> Iaquinta, Fiorella.]]> Pistón, Mariela.]]> Machado, Ignacio.]]> Información sobre Derechos de Autor

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ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> https://doi.org/10.1016/j.lwt.2021.112027]]> Determination of Cd, Pb and Se in beef samples using aerosol dilution by ICP-MS]]> An analytical method for sample preparation and subsequent determination of Cd, Pb and Se in beef using aerosol dilution by inductively coupled plasma-mass spectrometry was developed. The use of high matrix introduction technology allowed the introduction of a complex sample into the plasma with higher content of total dissolved solids a residual acidity of 4% and better tolerance to matrix effects. Trueness was verified by the analysis of two reference materials. At the 95% significance level, results were statistically equivalent to the certified or informed values. Analytical precision expressed as relative standard deviation was less than 9% in all cases. After validation, a total of 12 beef samples from Uruguay were analyzed. Obtained levels for Cd and Pb were far below the maximum limits established by international regulations in all samples. Also, results showed that beef from this region is a good source of Se in the range 0.120–0.870 mg kg−1 with an interesting contribution to the daily intake of this essential element.]]> Iaquinta, Fiorella.]]> Lopes Fialho, Lucimar]]> Nóbrega, Joaquín A.]]> Pistón, Mariela.]]> Machado, Ignacio.]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1007/s12161-020-01861-w]]> Antimicrobial peptides in the seedling transcriptome of the tree legume Peltophorum dubium

]]> PEPTIDOS ]]> CISTEINA]]> ANTIMICROBIANOS]]> Antimicrobial peptides (AMPs) play an essential role in plant defense against invading pathogens. Due to their biological properties, these molecules have been considered useful for drug development, as novel agents in disease therapeutics, applicable to both agriculture and medicine. New technologies of massive sequencing open opportunities to discover novel AMP encoding genes in wild plant species. This work aimed to identify cysteine-rich AMPs from Peltophorum dubium, a legume tree from South America. We performed whole-transcriptome sequencing of P. dubium seedlings followed by de novo transcriptome assembly, uncovering 78 AMP transcripts classified into five families: hevein-like, lipid-transfer proteins (LTPs), alpha hairpinins, defensins, and snakin/GASA (Giberellic Acid Stimulated in Arabidopsis) peptides.
No transcripts with similarity to cyclotide or thionin genes were identified. Genomic DNA analysis by PCR confirmed the presence of 18 genes encoding six putative defensins and 12 snakin/GASA peptides and allowed the characterization of their exon-intron structure. The present work demonstrates that AMP prediction from a wild species is possible using RNA sequencing and de novo transcriptome assembly, regarding a starting point for studies focused on AMP gene evolution and expression. Moreover, this study allowed the detection of strong AMP candidates for drug development and novel biotechnological
products.

]]> Rodríguez Decuadro, Susana Beatriz.]]> da Rosa, Gabriela.]]> Radío, Santiago.]]> Barraco Vega, Mariana.]]> Benko Issepon, Ana Maria.]]> Dans, Pablo D.]]> Smircich, Pablo. ]]> Cecchetto, Gianna.]]> Biochimie v.180.2021. -- p.1-14]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> Contaminaçao por chumbi no uruguai e o caso do bairro "La Teja"]]> Mañay, Nelly]]>

Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

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