Elaboration of goat cheese with increased content of conjugated linoleic acid and transvaccenic acid: Fat, sensory and textural profile ]]> Goat`s milk and its dairy products have been commonly related to important nutritional and health benefits. In fact, fat fraction is one of the most important components of goat’s milk regarding its value, as lipids are involved in cheese yield, firmness, color and flavor of goat dairy products. The aim of this study was to evaluate the modification in the lipid and sensory profile of cheeses elaborated with goat milk, through diet inclusion of sunflower oil (SFO). The main objective was to elaborate Danbo Type cheeses with increased content of conjugated linoleic acid (CLA) and transvaccenic acid (TVA), and at the same time decrease the proportion of saturated fat, and evaluate these values during the shelf life of the cheeses. The study was conducted with 60 Saanen goats separated into two homogeneous groups and supplemented for 18 days with one of two concentrates: without added oil (control, C) or enriched with sunflower oil (SFO). Cheeses were elaborated using goat milk, collected after desirable changes in its lipid profile were achieved. Physicochemical, microbiological, and
fatty acid composition together with the sensory profile were evaluated. In SFO cheeses fat content increased, without affecting protein content, microbiological, sensory profile and consumer acceptability. Also, the content of TVA increased from 2.4 to 4.4 % (p < 0.01), while CLA presented an increase from 0.8 to 1.3 % (p < 0.01). A decrease in the saturated/unsaturated ratio was observed resulting in a decrease in the atherogenic index. Therefore, inclusion of SFO in the diet of goats could be a viable alternative to increase the contribution of fatty acids with beneficial effects for health in cheeses.

]]> Dauber, Cecilia]]> Carreras, Tatiana]]> Britos, Alejandro]]> Carro, Silvana]]> Cajarville, Cecilia]]> Gámbaro, Adriana]]> Jorcin, Santiago]]> López, Tomas]]> Vieitez, Ignacio]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

]]> 10.1016/j.smallrumres.2021.106379 ]]> New perspective on thermally stimulated luminescence and crystallization of barium borate oxyfluoride glasses]]> The demand for modern materials, especially glasses, used in different applications, such as radiation sensors and spectral converters, requires a detailed study of their properties. The incorporation of fluoride compounds in borate glasses and their crystallization at the nanometric scale allows the properties of these materials to be further enhanced. Although many works showed improvements in some of these properties, some critical aspects, such as the crystallization mechanism and
the role of the fluorine phase, need more investigation. We worked with xNaF (100 - x)BaO.2B2O3 glasses with x = 0, 5, 10, 15, 20, 25, 30, and 35% (in mol) to increase the knowledge in this field. The structural modifications and the thermally stimulated luminescence of the glasses were studied,
and their crystallization was analyzed by thermal analysis and X-ray diffraction. A continuous trap distribution was found, which was responsible for its very good luminescent signal, especially in glasses with 20% NaF. By selecting a suitable amount of NaF, it is possible to obtain nanocrystals of
BaF2. These promising results we reached show the applicability of these materials.

]]> Rodríguez Chialanza, Mauiricio]]> Azcune, Germán]]> Bentos Pereira, Heinkel]]> Faccio, Ricardo]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.3390/cryst11070745 ]]> Docetaxel in chitosan-based nanocapsules conjugated with an anti-Tn antigen mouse/human chimeric antibody as a promising targeting strategy of lung tumors]]> The aim of this work was to evaluate the physicochemical and biological properties of docetaxel (DCX) loaded chitosan nanocapsules (CS Nc) functionalized with the monoclonal antibody Chi-Tn (CS-PEG-ChiTn mAb Nc) as a potential improvement treatment for cancer therapy. The Tn antigen is highly specific for carcinomas, and this is the first time that such structure is targeted for drug delivery. The nanocapsules (Ncs), formed as a polymeric shell around an oily core, allowed a 99.9% encapsulation efficiency of DCX with a monodispersity particle size in the range of 200 nm and a high positive surface charge that provide substantial stability to the nanosystems. Release profile of DCX from Ncs showed a sustained and pH dependent behavior with a faster release at acidic pH, which could be favorable in the intracellular drug delivery.We have designed PEGylated CS Nc modified with amonoclonal antibody which recognize Tn antigen, one of themost specific tumor associated antigen. A biotin-avidin approach achieved the successful attachment of the antibody to the nanocapsules. Uptake studies and viability assay conducted in A549 human lung cancer cell line in vitro demonstrate that ChiTn mAb enhance nanoparticles internalization and cell viability reduction. Consequently, these ChiTn functionalized nanocapsules are promising carriers for the active targeting of DCX to Tn expressing carcinomas.

]]> Castro, Analía]]> Berois, Nora]]> Malanga, Antonio]]> Ortega, Claudia]]> Oppezzo, Pablo]]> Pristch, Otto]]> Mombrú, Álvaro W.]]> Osinaga, Eduardo]]> Pardo, Helena]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.1016/j.ijbiomac.2021.04.054 ]]> Development and characterization of two novel 68 Ga labelled neuropeptide Y short analogues with potential application in breast cancer imaging ]]> In vivo receptor targeting with radiolabelled peptide-based probes is an attractive approach for the development of novel radiotracers for molecular imaging. This work presents the development and characterization of two novel neuropeptide Y analogues labelled with a positron emitter 68Ga, for potential use in breast cancer imaging. Both analogues share the same amino acid sequence and were derivatized with NOTA through either a lysine linker (L1) or an acetylated lysine (L2). In both cases, a single product with radiochemical purity higher than 95% was obtained. The two complexes were hydrophilic, showed remarkable in vitro stability, good cellular uptake, binding affinity in the nanomolar range and high cellular internalization rate. Biodistribution studies revealed low blood uptake and elimination through the urinary tract. The addition of an acetyl group in the spacer increased the lipophilicity of C2 and modified the reactivity of the ε-amino group of the lysine which resulted in lower protein binding and lower percentage of injected dose in bladder and urine. The tumour versus muscle ratio was (3.8 ± 0.4) for 68Ga-L1 and (4.7 ± 0.4) for 68Ga-L2. These results encourage performing further studies in order to complete the evaluation of both tracers as potential radiopharmaceutical for breast cancer imaging.]]> Cardoso, Maria Elena]]> Tejería, Emilia]]> Zirbesegger, Kevin]]> Savio, Eduardo]]> Terán, Mariella]]> Rey Ríos, Ana María]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.1111/cbdd.13864 ]]> Implications of climate change: how does increased water temperature influence biofilm and water quality of chlorinated drinking water distribution systems?]]> Temperature variation can promote physico-chemical and microbial changes in the water transported through distribution systems and influence the dynamics of biofilms attached to pipes, thus contributing to the release of pathogens into the bulk drinking water. An experimental real-scale chlorinated DWDS was used to study the effect of increasing temperature from 16 to 24C on specific pathogens, bacterialfungal
communities (biofilm and water samples) and determine the risk of material accumulation and mobilisation from the pipes into the bulk water. Biofilm was developed for 30 days at both temperatures in the pipe walls, and after this growth phase, a
flushing was performed applying 4 gradual steps by increasing the shear stress. The fungal-bacterial community characterised by Illumina MiSeq sequencing, and specific pathogens were studied using qPCR: Mycobacterium spp., Mycobacterium avium
complex, Acanthamoeba spp., Pseudomonas aeruginosa, Legionella pneumophilia, and Stenotrophomonas maltophilia. Sequencing data showed that temperature variation significantly modified the structure of biofilm microbial communities from the early stages of biofilm development. Regarding bacteria, Pseudomonas increased its relative abundance in biofilms developed at 24C, while fungal communities showed loss of diversity and richness, and the increase in dominance of Fusarium genus.
After the mobilisation phase, Pseudomonas continued being the most abundant genus at 24° C, followed by Sphingobium and Sphingomonas. For biofilm fungal communities after the mobilisation phase, Helotiales incertae sedis and Fusarium were
the most abundant taxa. Results from qPCR showed a higher relative abundance of Mycobacterium spp. on day 30 and M. avium complex throughout the growth phase within the biofilms at higher temperatures. The temperature impacts were not
only microbial, with physical mobilisation showing higher discolouration response and metals release due to the increased temperature. While material accumulation was
accelerated by temperature, it was not preferentially to either stronger or weaker biofilm layers, as turbidity results during the flushing steps showed. This research yields new understanding on microbial challenges that chlorinated DWDS will undergo as global temperature rises, this information is needed in order to protect drinking water quality and safety while travelling through distribution systems.

]]> Calero Preciado, Carolina]]> Boxall, Joby]]> Soria Carrasco, Víctor]]> Martínez, Soledad]]> Douterelo, Isablel]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.3389/fmicb.2021.658927 ]]> The contributions of civil society to food security in the context of COVID-19: a qualitative exploration in Uruguay]]> Objective: The aim of this study was to explore and analyse the actions implemented by civil society to contribute to food security in the context of the COVID-19 outbreak in Uruguay, a high-income country in South America.
Design: An exploratory systematic approach was used to identify the contributions of civil society to food security through reports in news websites and Facebook
posts. Data were analysed based on content analysis following a deductive–inductive approach.
Setting: Uruguay, Latin America.
Results: A total of 1220 civil society organisations were identified, which developed two main actions to increase access to food among the Uruguayan population: food baskets and ‘community pots’ (also known as ‘common pots’). Most of the
initiatives targeted citizens under socioeconomic vulnerability in the face of COVID-19, without specifying any specific requirement or population segment.
Actions were mainly led by spontaneously organised community groups, and, to a lesser extent, by consolidated organisations. Interactions between organisations were identified. The foods provided by the organisations were mostly aligned with national dietary guidelines. Social media posts evidenced that the main challenge faced by organisations was related to the lack of funds or supplies.
Conclusions: Results from this work suggest that the lack of funds or supplies poses challenges to the medium- and long-term contributions of civil society to food security and stresses the need for comprehensive governmental measures to guarantee food security amongst Uruguayan citizens.

]]> Brunet, Gerónimo]]> Girona, Alejandra]]> Fajardo, Gabriela]]> Iragola, Valentina]]> Machín, Leandro]]> Bove, Isabel]]> Ares, Gastón]]> Cambridge University Press]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.1017/S1368980021003463 ]]> Effect of UV-C Irradiation and Lactic Acid Application on the Inactivation of Listeria monocytogenes and Lactic Acid Bacteria in Vacuum-Packaged Bee]]> The objective of this study was to test the effect of the combined application of lactic acid (0–5%) (LA) and UV-C light (0–330 mJ/cm2) to reduce Listeria monocytogenes and lactic acid bacteria (LAB) on beef without major meat color (L *, a *, b *) change and its impact over time. A two-factor central composite design with five central points and response surface methodology (RSM) were used to optimize LA concentration and UV-C dose using 21 meat pieces (10 g) inoculated with
L. monocytogenes (LM100A1). The optimal conditions were analyzed over 8 weeks. A quadratic model was obtained that predicted the L. monocytogenes log reduction in vacuum-packed beef treated with LA and UV-C. The maximum log reduction for L. monocytogenes (1.55 ± 0.41 log CFU/g) and
LAB (1.55 ± 1.15 log CFU/g) with minimal impact on meat color was achieved with 2.6% LA and 330 mJ/cm2 UV-C. These conditions impaired L. monocytogenes growth and delayed LAB growth by 2 weeks in vacuum-packed meat samples throughout 8 weeks at 4° C. This strategy might contribute to improving the safety and shelf life of vacuum-packed beef with a low impact on meat color.

]]> Brugnini, Giannina]]> Rodríguez, Soledad]]> Rodríguez, Jesica]]> Rufo, Caterina]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.3390/foods10061217 ]]> Microcystin ELISA in water and animal serum for an integrated environmental monitoring strategy]]> Free-range livestock can be exposed to toxic cyanobacteria by drinking water from eutrophic natural sources. This study reports on the analysis of livestock drinking water sources from ranches in an eutrophic subtropical basin of South America (33ºS, 57°W), where frequent Microcystis spp. blooms and occasionally dense scums were found. To minimise monitoring costs, microcystins (MCs) were determined in serum and water with an in-house developed enzyme linked immunosorbent assay (ELISA). The water results were compared with those of liquid chromatography – tandem mass spectrometry (LC-MS/MS) for the most common congeners (MC-LR, MC-RR; MC-YR, MC-LA). Both methods were strongly correlated along a broad range of concentrations. The concentrations of MCs in the livestock drinking water were up to 3,700 μg/L. This is about 1,000 times higher than the Australian and New Zealand livestock MCs guidance values (cattle = 4.2; sheep = 3.9 μg/L) as well as the California-EPA sub-chronic action level (3 μg/L) and implies a high risk of poisoning. The animals exposed to MCs concentrations 70–100 times higher than these guidelines showed no significant alterations in liver enzymes. Nevertheless, serum concentrations of up to 0.11 μg/L MCs were recorded by ELISA. These results reveal the potential of MCs-ELISA in serum as a sensitive, minimally invasive method for risk assessment in integrated environmental monitoring strategies.

]]> Brena, Beatriz M.]]> Font, Elizabeth]]> Pírez Schirmer, Macarena]]> Badagian, Natalia]]> Cardozo, Elena]]> Pérez Parada, Andrés]]> Bonilla, Sylvia]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1080/03067319.2021.1881073 ]]> Study of emulsifying properties of soluble proteins obtained from defatted rice bran concentrate ]]> Rice (Oryza sativa) is one of the most frequently produced cereals in the world. Rice bran (RB) is obtained as a by-product in the rice milling process. A part of the RB is used for oil extraction, obtaining defatted rice bran as a second by-product.
The aim of this work was to analyze the emulsifying properties of soluble proteins fraction present in defatted rice bran concentrate (DRBC) in acidic and neutral conditions. Fine emulsions (prepared by high-speed and ultrasound
homogenization) stabilized with soluble proteins obtained from DRBC showed a mean particle size lower than coarse emulsions (prepared by only high-speed homogenization) and a significantly lower degree of overall destabilization. Coarse emulsions showed sigmoidal destabilization profiles at pH 4.5 and 7.0, related to the existence of two populations with different particle sizes. In fine emulsions, both pH provided lower particle sizes and greater stability. After 24 h
of quiescent storage, the coarse emulsions showed an increase of particle size that was not observed in fine emulsions. Both types of emulsions showed a Newtonian-type behavior. Fine emulsions showed higher viscosity values and higher lightness than coarse emulsions, consistent with the reduction of particle
size and increase in the number of particles. The soluble proteins obtained from a waste of the rice industry can be used to obtain stable fine oil-in-water emulsions in acidic and neutral conditions. This result is of interest since it could be used as
a food ingredient, increasing the added value of this important by-product.

]]> Bonifacino, Carla]]> Palazolo, Gonzalo]]> Panizzolo, Luis A.]]> Abirached, Cecilia]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.]]> 10.1002/aocs.12518]]> Revalorization of chicken feather waste into a high antioxidant activity feather protein hydrolysate using a novel psychrotolerant bacterium]]> In this study, we assessed the capacity of Pedobacter sp. 3.14.7 —a novel Antarctic keratinolytic bacterium— of producing a feather protein hydrolysate (FPH) with high antioxidant activity. This strain was able to degrade raw feather into protein hydrolysate under optimal conditions of 30 g l- 1 of feather concentration, pH 6.0, and 20 ◦C. Maximum enzymatic activity was detected after a 192-h cultivation period (125.67 ± 3.06 U ml- 1). Quenching of the ABTS radical (2,20-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid) by the hydrolysate reached a value of approximately 95%, with a 50% Inhibitory Concentration (IC50) value of 0.57 ± 0.01 g l- 1. An interesting reducing power of the FPH towards Fe+3 was also detected (2.19 Abs700 units in the presence of 2.03 g - 1 protein). The hydrolysate also displayed a moderate nitric oxide-scavenging activity, with an IC50 of 0.53 ± 0.01 g l- 1. This FPH’s free amino acid content is high (almost 5 g l- 1). Results indicate that this hydrolysate may be useful as supplementary protein and antioxidant in animal feed formulations.

]]> Bezus, Brenda]]> Ruscasso, Florencia]]> Garmendia, Gabriela]]> Vero, Silvana]]> Cavello, Ivana]]> Cavalitto, Sebastián]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> 10.1016/j.bcab.2021.101925 ]]> Single-Molecule Vibrational Spectroscopy: Cobalt Tetraphenylporphyrin Deposited on a Cu2N Ultrathin Insulating Layer ]]> The electronic and vibrational properties of cobalt tetraphenylporphyrin (CoTPP) molecules deposited onto a
Cu2N−Cu(100) surface were studied by scanning tunneling microscopy. The Cu2N spacer layer promoted the decoupling between the localized electronic states of CoTPP and the metallic substrate, enabling the observation of electronic states of nearly free molecules. The experimental results are supported by ab initio calculations, and the correspondence with C−H and C−C bonding
vibrations detected by inelastic electron tunneling spectroscopy (IETS) has been shown. For molecules adsorbed at the interface
between Cu2N and Cu, the IETS signal was also observed.

]]> Baggio, Bruna F.]]> Chen, Weijiong]]> Montenegro, Benjamín]]> Tumelero, Milton A.]]> Faciio, Ricardo]]> Zoldan, Vinicius C.]]> Gao, Chunlei]]> Pasa, André A.]]> American Chemical Society]]> Información sobre Derechos de Autor (Por favor lea este aviso antes de abrir los documentos u objetos)

]]> https://doi.org/10.1021/acs.jpcc.0c08722]]> Expression and distribution of glutathione transferases in protoscoleces of Echinococcus granulosus sensu lato]]> Glutathione transferases (GSTs) belong to a diverse superfamily of multifunctional proteins involved in metabolic detoxification. In helminth parasite, GSTs are particularly relevant since they are also involved in host immunomodulation. Echinococcus granulosus sensu lato (s.l.) is a cestode parasite known to express at least three phylogenetically distant cytosolic GSTs: EgGST1 and EgGST2 previously grouped within Mu and Sigma classes, respectively; and EgGST3 related to both Omega and Sigma classes. To better characterize E. granulosus s.l. GSTs, herein their expression and distribution were assessed in the pre-adult protoscolex (PSC) parasite stage. Potential transcriptional regulatory mechanisms of the corresponding EgGST genes were also explored. Firstly, the transcription of the three EgGSTs was significantly induced during the early stages of the murine model of infection, suggesting a potential role during parasite establishment. EgGST1 was detected in the parenchyma of PSCs and its expression increased after H2O2 exposure, supporting its role in detoxification. EgGST2 was mainly detected on the PSCs tegument, strategically localized for potential immunoregulation functions due to its Sigma-class characteristics. In addition, its expression increased after anthelmintic treatment, suggesting a role in chemotherapy
resistance. Finally, the Omega-related EgGST3 was localized throughout the entire PSC body, including suckers and tegument, and since its expression also increased after H2O2 treatment, a potential role in oxidative stress response could also be ascribed. On the other hand, known cis-acting regulatory motifs were detected in EgGST genes, suggesting similar transcription processes to other eukaryotes. The results herein reported provide additional data regarding the roles of EgGSTs in E. granulosus s.l. biology, contributing to a better understanding of its host-parasite interaction.

]]> Arbildi, Paula]]> La Rocca, Silvana]]> Kun, Alejandra]]> Lorenzatto, Karina R.]]> Monteiro, Karina M.]]> Zaha, Arnaldo]]> Mourglia-Ettlin, Gustavo]]> Ferreira, Henrique B.]]> Fernández, Verónica]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)

ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

]]> Synthesis, characterization, 31P{1H} NMR, X-ray structure and voltammetric study of 2-mercaptothiazolines and phosphines ruthenium(II) complexe]]> Appelt, Patrícia]]> de Lara, Camila M.]]> da Silva, Juliana P.]]> da Silva, Janaína S.]]> Sandrino, Bianca]]> Alvarez, Natalia]]> Ellena, Javier A.]]> Back, Davi F.]]> de Araujo, Márcio P.]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

]]> The Lymphocytic Scavenger Receptor CD5 Shows Therapeutic Potential in Mouse Models of Fungal Infection]]> Invasive fungal diseases represent an unmet clinical need that could benefit from novel immunotherapeutic approaches. Host pattern recognition receptors (e.g., Toll-like receptors, C-type lectins, or scavenger receptors) that sense conserved fungal cell wall constituents may provide suitable immunotherapeutic antifungal agents. Thus, we explored the therapeutic potential of the lymphocyte class I scavenger receptor CD5, a nonredundant component of the antifungal host immune
response that binds to fungal -glucans. Antifungal properties of the soluble ectodomain of human CD5 (shCD5) were assessed in vivo in experimental models of systemic fungal infection induced by pathogenic species (Candida albicans and Cryptococcus neoformans). In vitro mechanistic studies were performed by means of fungus-spleen cell cocultures. shCD5-induced survival of lethally infected mice was dose and time dependent and concomitant with reduced fungal load and increased leukocyte infiltration in the primary target organ. Additive effects were observed in vivo after shCD5 was combined with suboptimal doses of fluconazole. Ex vivo addition of shCD5 to fungus-spleen cell cocultures increased the release of proinflammatory cytokines involved in antifungal defense (tumor necrosis factor alpha and gamma interferon) and reduced the number of viable C. albicans organisms. The results prompt further exploration of the adjunctive therapeutic potential of shCD5 in severe invasive fungal diseases.

]]> Velasco de Andrés, María]]> Català, Cristina]]> Casadó Llombart, Sergi]]> Martínez Florensa, Mario]]> Simões, Inês]]> García Luna, Joaquín]]> Mourglia Ettlin, Gustavo]]> Zaragoza, Óscar]]> Carreras, Esther]]> Lozanoa, Francisco]]> Información sobre Derechos de Autor

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]]> https://doi.org/10.1128/AAC.01103-20 ]]> Estructura electrónica del benzofurazano y algunos derivados - estado fundamental]]> Krebs, Germán]]> Sosa, Ramón]]> Información sobre Derechos de Autor

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]]> Estructura electrónica del benzofurazano y algunos derivados – espectros electrónicos.

]]> HISTORIA

]]> Krebs, Germán]]> Sosa, Ramón]]> XI Congreso Latinoamericano de Química, Universidad Católica de Chile]]> Información sobre Derechos de Autor

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]]> Estudio mecánico-cuántico de algunos equilibrios cetona-énol.]]> Se estudia la correlación entre constantes de equilibrio experimentales ceto-enol y magnitudes calculadas por métodos mecánico-cuánticos para algunas cetonas.

Los cálculos involucran energías por el método ENT (Extended Nuekel Theory) y funciones termodinámicas en la aproximación rotor rígido-oscilador armónico.

]]> Krebs, Germán]]> Sosa, Ramón]]> Información sobre Derechos de Autor

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]]> Programa para mejorar resolución de espectros]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

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]]> Obtención de gráficos de densidad electrónica molecular (pi).]]> Krebs, Germán]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

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]]> Programa para la representación 3D con el plotter del Centro de Cómputos del CCUR]]> Krebs, Germán]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

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]]> Cálculo de coordenadas y distancias en moléculas tridimensionales]]> Se construye un método sistemático de cálculo de las coordenadas atómicas en moléculas tridimensionales.

Se utilizan versores dirigidos a lo largo de los enlaces, un sistema de coordenadas esféricas asociadas a cada átomo y operaciones de simetría.

La subrutina Fortran programada se probó en el Sistema IBM 360/44 del Centro de Computación de la Universidad. Este cálculo de coordenadas se utiliza luego en los programas de química cuántica.

]]> Krebs, Germán]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

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]]> Determinación de sodio, potasio y calcio en muestras de feldespatos del orden del miligramo.

1) Por espectrofotometría de llama.

2) Por espectrografía de arco.

]]> Con objeto de determinar cuantitativamente y en forma simultánea sodio, potasio y calcio, en muestras únicas de feldespatos, del orden del miligramo, se ponen en marcha dos técnicas. Una de ellas utiliza espectrofotometría de llama y la otra espectrografía de arco.

La primera, de manipulación más simple que la segunda, permite determinar los porcentajes en óxido con un error máximo del 25 %, y un error promedial de 15 %.

La segunda (con "standard interno" y "buffer espectrográfico") da un error máximo que oscila entre 30% y 40 % según el elemento. El error promedial es del 20 %. Esta última, trabajando con triplicados (cuando se dispone de muestra suficiente) permite errores del orden del 6% al 10%.

En muestras de este orden (1 mg), en general, no se puede esperar más que un semicuantitativo, pues aunque se buscan coponentes mayores, dada la pequeñez de la muestra, el problema equivale a una determinación de trazas. Esto hace que los resultados obtenidos se pueden considerar aceptables, si no se dispone de medios técnicos más adecuados.

]]> Krebs, Germán]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

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]]> (I) Regresión y correlación lineal (II) Regresión cuadrática – Programación en Fortran IV.]]> Krebs, Germán]]> Presto, Jorge]]> Feldmann, Eduardo]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

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]]> Programa en Fortran para el estudio de moléculas por el método LCAO simple (Método de Hückel).

]]> Se hizo un programa en Fortran IV para realizar cálculos por el método LCAO (combinación lineal de orbitales atómicos) simple, o método de Hückel.

Los resultados de este programa son los valores y vectores propios, de los cuales se obienen las densidades de carga electrónica y los ordenes de enlace, además de la energía π total y la deslocalización.

Por supuesto, estos valores pueden convertirse en momeno dipolar, reactividad química, energía de transicion, etc.

El porgrama se probó en el Sistema IBM 360/44 del Centro de Comptación de la Universidad.

Se dejan ejemplos de la utilización del programa.

]]> Krebs, Germán]]> Montevideo : UdelaR-Facultad de Química]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

]]> Cálculo de coordenadas y distancias en moléculas planas – Teoría y programa en Fortran.]]> Se utiliza un metódo de cálculo, basado en los productos escalar y vectorial de versores dirigidos a lo largo de una poligonal trazada sobre los enlaces de una molécula plana, para realizar una rutina de cálculo, en FORTRAN IV, que permite determinar las coordenadas de todos los átomos y las distancias entre un átomo dado y todos los demás, aparte de otros parámetros geométricos.

En el cálculo, se hace uso de los elementos de simetría de la molécula en cuestión.

La subrturina se probó en el Sistea IBM 360/44 del Centro de Computación de la Universidad, estando integrada actualmente a nuestros progrmas de cálculos mécanico-cuánticos.

]]> Krebs, Germán]]> Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

]]>