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                <text>Some isodesmic reactions, involving the fluorine oxide radical FO, have been studied employing density functional theory (DFT) and coupled-cluster (CC) calculations with an extended, uncontracted basis set. It is shown that CCSD(T) calculations can give more accurate enthalpies of reaction than DFT in some of the non-isodesmic reactions. DFT, however, gives more accurate results than CCSD(T) for the isodesmic reactions considered.</text>
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                <text>The standard singles and doubles coupled-cluster method including perturbational treatment of connected triple excitations, CCSD(T), and density functional methods, DFT, using a large, uncontracted, atomic natural orbital (ANO) basis set were employed for calculating the enthalpies of formation of some first-row atoms (H, N, O, F) as well as diatomic and triatomic molecules formed with them. Molecular enthalpies of formation at 298.15 K were obtained from enthalpies of reaction of the atoms, homonuclear diatomic molecules, and isodesmic reactions. It is shown that the errors are the minimum when isodesmic reactions are used. However, contrary to accepted belief, CCSD(T) gives a larger deviation from experiment than DFT in the latter case. DFT exhibits similar accuracy when using a very small basis set (6-31G*) to that with the extended basis set, while the errors obtained with CCSD(T) are much larger. It is concluded that DFT is a more accurate and convenient computational tool than CCSD(T) for the thermochemical study of species containing the F&amp;minus;O bond.</text>
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                <text>Light chain deposition disease (LCDD) and light chain amyloidosis (AL) are disorders of monoclonal immunoglobulin deposition in which normally soluble serum precursors form insoluble deposits in tissues. A common feature in both is the clonal proliferation of B-cells that produce pathogenic light chains. However, the deposits in LCDD differ from those in AL in that they are ultrastructurally granular rather than fibrillar and do not bind Congo red or colocalize with amyloid P component or apolipoprotein E. The reason(s) for their differences are unknown but are likely multifactorial and related to their protein conformation and their interaction with other molecules and tissue factors in the microenvironment. Knowledge of the primary structure of the light chains in LCDD is very limited. In the present study two new &amp;kappa;1 light chains from patients with LCDD are described and compared to seven other reported &amp;kappa;-LCDD proteins. The N-terminal amino acid sequences of light chain GLA extracted from the renal biopsy and light chain CHO from myocardial tissue were each identical to the respective light chains isolated from the urines and to the V-region amino acid sequences translated from the cloned cDNAs obtained from bone marrow cells. The germline V-region sequences, determined from the genomic DNA in both and in MCM, a previously reported &amp;kappa;1 LCDD light chain, were identical and related to the L12a germline gene. The expressed light chains in all three exhibit amino acid substitutions that arise from somatic mutation and result in increased hydrophobicity with the potential for protein destabilization and disordered conformation.</text>
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                <text>&lt;strong&gt;Vidal, R&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Go&amp;ntilde;i, Fernando&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Stevens, F&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Aucouturier, P&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Kumar, A&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Frangione, B&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Ghiso, J&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Gallo, G&lt;/strong&gt;</text>
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                <text>American Journal of Pathology v. 155, no. 6, 1999. -- p. 2009-2017</text>
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                <text>Elsevier</text>
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            <name>Rights</name>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Ingl&amp;eacute;s</text>
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        <name>Proteínas</name>
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                  <text>Bibliografía Nacional Química</text>
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              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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          <name>Original Format</name>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>&lt;strong&gt; IgG recognizing 21-24 KDa and 30-33kDa tachyzoite antigens show maximum avidity maturation during natural and accidental human toxoplasmosis.&lt;/strong&gt;</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>ANTIGENOS</text>
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                <text>TOXOPLASMOSIS</text>
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                <text>BIBLIOGRAFIA NACIONAL QUIMICA</text>
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                <text>1999</text>
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          <element elementId="87">
            <name>Abstract</name>
            <description>A summary of the resource.</description>
            <elementTextContainer>
              <elementText elementTextId="319202">
                <text>We describe the avidity maturation of IgGs in human toxoplasmosis using sequential serum samples from accidental and natural infections. In accidental cases, avidity increased continuously throughout infection while naturally infected patients showed a different profile. Twenty-five percent of sera from chronic patients having specific IgM positive results could be appropriately classified using exclusively the avidity test data. To take advantage of the potentiality of this technique, antigens recognized by IgG showing steeper avidity maturation were identified using immunoblot with KSCN elution. Two clusters of antigens, in the ranges of 21-24 kDa and 30-33 kDa, were identified as the ones that fulfill the aforementioned avidity characteristics.</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
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                <text>&lt;strong&gt;Villavedra, M&lt;/strong&gt;</text>
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              <elementText elementTextId="319204">
                <text>&lt;strong&gt;Battistoni Spinelli, Julio Jos&amp;eacute;&lt;/strong&gt;</text>
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                <text>&lt;a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8"&gt;&lt;strong&gt;Nieto, Alberto&lt;/strong&gt;&lt;/a&gt;</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="319206">
                <text>Revista do Instituto de Medicina Tropical de S&amp;atilde;o Paulo v. 41, no. 5, 1999. -- p. 255-62</text>
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          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="319207">
                <text>Instituto de Medicina Tropical</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>1999</text>
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            <name>Rights</name>
            <description>Information about rights held in and over the resource</description>
            <elementTextContainer>
              <elementText elementTextId="319209">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>El presente trabajo tiene como principal objetivo la obtenci&amp;oacute;n y caracterizaci&amp;oacute;n de un biocatalizador en fase s&amp;oacute;lida de aplicaci&amp;oacute;n en la hidr&amp;oacute;lisis de la lactosa de un efluente de la industria lechera. Con este prop&amp;oacute;sito se optimiz&amp;oacute; la inmovilizaci&amp;oacute;n de una preparaci&amp;oacute;n comercial de b-galactosidasa de A.oryzae(Biolactase)por adsorci&amp;oacute;n i&amp;oacute;nica en DEAE-Sepharose CL-6B. Cuando se incub&amp;oacute; una soluci&amp;oacute;n de enzima con esta resina bajo las condiciones &amp;oacute;ptimas encontradas se alcanz&amp;oacute; un rendimiento de inmovilizaci&amp;oacute;n del 99. La actividad expresada por el derivado as&amp;iacute; obtenido fue del 40-66 y los valores de Km y Vm&amp;aacute;x aparentes del mismo para el sustrato artificial ONPG fueron de 19.7 mM y 70 mmol/min Mg. El biocatalizador mantuvo el 100 de su actividad inicial durante dos meses de almacenamiento a 4oC y su actividad se redujo al 50 al cabo de 34 d&amp;iacute;as a 25oC se alcanzaron grados de conversi&amp;oacute;n del orden de 80 manteni&amp;eacute;ndose el total de la actividad al cabo del proceso. Se concluye que tanto la relaci&amp;oacute;n lineal encontrada entre carga enzim&amp;aacute;tica aplicada y actividad expresada como la alta capacidad de la resina permiten la obtenci&amp;oacute;n de derivados activos y estables de utilidad en procesos de lactolisis.</text>
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                <text>&lt;strong&gt;Zamisch, M&lt;/strong&gt;</text>
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                <text>&lt;a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8dfa5283acaafc5c73dab5b61b04f9a0"&gt;&lt;strong&gt;Batista-Viera, Francisco&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>Informaci&amp;oacute;n Tecnol&amp;oacute;gica v. 10, no. 3, 1999. -- p. 105-110</text>
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                <text>Centro de Informaci&amp;oacute;n Tecnol&amp;oacute;gica</text>
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                <text>&lt;strong&gt;&lt;strong&gt;&lt;/strong&gt;&lt;/strong&gt;&#13;
&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Ingl&amp;eacute;s</text>
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                <text>ISSN: 0716-8756</text>
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        <name>Beta-galactosidasa</name>
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        <name>Inmovilización de enzimas</name>
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                  <text>Bibliografía Nacional Química</text>
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              <description>An account of the resource</description>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>&lt;strong&gt;Decreased azithromycin susceptibility of Neisseria gonorrhoeae due to mtrR mutations.&lt;/strong&gt;</text>
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            <description>The topic of the resource</description>
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                <text>AZITROMICINA</text>
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                <text>ANTIINFECCIOSOS</text>
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                <text>NEISSERIA GONORRHOEAE</text>
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                <text>BIBLIOGRAFIA NACIONAL QUIMICA</text>
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            <description>A summary of the resource.</description>
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                <text>Single-dose azithromycin therapy has recently been used in Uruguay for the treatment of uncomplicated gonococcal infections. As part of an active surveillance study to monitor the emergence of antibiotic resistance in gonococcal isolates, we examined the levels of azithromycin susceptibility in 51 consecutive isolates obtained from males with uncomplicated gonococcal urethritis. Isolates with decreased susceptibility to azithromycin (MICs, 0.25 to 0.5 &amp;mu;g/ml) were common, and these isolates often displayed cross-resistance to hydrophobic antimicrobial agents (erythromycin and Triton X-100). Resistance to erythromycin and Triton X-100 is frequently due to overexpression of the mtrCDE-encoded efflux pump mediated by mutations in themtrR gene, which encodes a transcriptional repressor that modulates expression of the mtrCDE operon. Accordingly, we questioned whether clinical isolates that express decreased azithromycin susceptibility harbor mtrR mutations. Promoter mutations that would decrease the level of expression ofmtrR as well as a missense mutation at codon 45 in themtrR-coding region that would result in a radical amino acid replacement within the DNA-binding motif of MtrR were found in these strains. When these mutations were transferred into azithromycin-susceptible strain FA19 by transformation, the susceptibility of gonococci to azithromycin was decreased by nearly 10-fold. The mtrCDE-encoded efflux pump system was responsible for this property since insertional inactivation of themtrC gene resulted in enhanced susceptibility of gonococci to azithromycin. We conclude that the mtrCDE-encoded efflux pump can recognize azithromycin and that the emergence of gonococcal strains with decreased susceptibility to azithromycin can, in part, be explained by mtrR mutations.</text>
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            <description>An entity primarily responsible for making the resource</description>
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                <text>&lt;strong&gt;Zarantonelli, L&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Borthagaray, Graciela&lt;/strong&gt;</text>
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                <text>&lt;strong&gt; Lee, E.H&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Shafer, W.M&lt;/strong&gt;</text>
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            <description>A related resource from which the described resource is derived</description>
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                <text>Antimicrobial Agents and Chemotherapy v. 43, no. 10, 1999. -- p. 2468-72</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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                <text>American Society of Microbiology</text>
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            <name>Date</name>
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                <text>1999</text>
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          <element elementId="47">
            <name>Rights</name>
            <description>Information about rights held in and over the resource</description>
            <elementTextContainer>
              <elementText elementTextId="319262">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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            <description>The file format, physical medium, or dimensions of the resource</description>
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            <description>The nature or genre of the resource</description>
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                <text>Doi: 0066-4804/99/$04.0010</text>
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                <text>The effects of I&amp;ndash;adsorption on the electrodeposition (under and overpotential deposition) of Cu on polycrystalline Pd electrodes were studied in dilute perchloric acid solutions at 18&amp;deg;C. It had been found that Cu underpotential deposition on polycrystalline Pd exhibits different potentiodynamic features, which are characteristic of defined crystallographic planes of Pd. However, these features varied when the voltammograms were performed in the presence of strongly adsorbable anions, such as I&amp;ndash;. In spite of having found a partial inhibition of the Cu voltammetric features in the presence of I&amp;ndash;, we calculated integer numbers in the electron transfer to Cu2+and I&amp;ndash;ions. The change in the values of Cu massive deposition potential due to the presence of I&amp;ndash;was caused by the appearence of a new electrode, that is, the Cu/CuI/I&amp;ndash;interface.</text>
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&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Electrochemical behavior and semiempirical approach to the electroadsorption of 3-buten-2-one and 2-butanone on platinum.&lt;/strong&gt;</text>
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                <text>The electroadsorption of 3-buten-2-one was studied on polycrystalline and faceted Pt electrodes in aqueous 0.5 M H(2)SO(4) between 20 and 70 degrees C using voltammetric and transient techniques. The largest values of anodic charge densities in stripping experiments were observed at the potential of zero charge of the Pt/acid interface (ca. 0.20 V), that is, 550, 680, and 720 &amp;micro;C cm(-2), on polycrystalline and (111)- and (100)-faceted electrodes, respectively. The anodic and cathodic strippings of 3-buten-2-one showed the formation of at least two residues with different electrochemical behavior. For comparison cyclic voltammetries and current transients of 2-butanone on Pt were also studied. To get a deeper insight into the adsorption configurations of 3-buten-2-one and 2-butanone, the extended H&amp;uuml;ckel molecular orbital methodology was used to predict adsorption geometries and binding energies on simulated Pt(100) and Pt(111) single-crystal surfaces. At equilibrium potentials, two types of adsorbed species were recognized, a planar 3-buten-2-one formed by the interaction of carbonyl and alkene groups and adsorbates coming from the single interaction of the &amp;gt;C&amp;amp;dbond;O moiety for both 3-buten-2-one and 2-butanone. At simulated positive potentials, 3-buten-2-one adsorption occurred on Pt(111) and Pt(100) clusters through the pi-orbitals of the &amp;gt;C&amp;amp;dbond;C&amp;lt; moiety, whereas at negative potentials 3-buten-2-one was adsorbed via &amp;micro;-bridging or di-varsigma configuration(s)</text>
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                <text>&lt;strong&gt;Zinola, C.F&lt;/strong&gt;</text>
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                <text>Journal of Colloid and Interface Science v. 219, no. 2, 1999. -- p. 260</text>
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                <text>&lt;strong&gt;&lt;strong&gt;&lt;/strong&gt;&lt;/strong&gt;&#13;
&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>To identify molecular markers of cell differentiation in developing nervous tissue, monoclonal antibodies against chick embryo neural retina were made. One of them, 3C3mAb, recognized a developmentally regulated antigen present in several organs of the CNS. Data from MALDI-TOF mass spectrometry and peptide sequencing of the immuno-affinity purified protein indicated identity of the antigen with MARCKS. The immunoreactive material was always found as a unique polypeptide (Mr 71 kDa) in SDS-PAGE, however isoelectrofocusing revealed the existence of several bands (pI ranging from 4.0 to 4.5). Interestingly some retinal cell types, as photoreceptors, exhibited an extremely significant decrease in the intensity of the immunoreactive material during the final phases of terminal differentiation while others, as some retinal neurons, maintained the immunoreactivity when fully differentiated. Taken together these results indicate that MARCKS, a protein susceptible of several posttranslational modifications as myristoylation and phosphorylation at variable extent, may act differently in neural retina cell types.</text>
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                <text>Biochemical and Biophysical Research Communications v.257, 1999. -- p. 480-487</text>
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                <text>Acta Crystallographica C v. C54, no. Parte12, 1998. -- p. 1902-1905</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;&#13;
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                <text>&lt;strong&gt;Diagnostic evaluation of a synthetic peptide derived from a novel antigen B subunit as related to other available peptides and native antigens used for serology of cystic hydatidosis.&lt;/strong&gt;</text>
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                <text>A synthetic peptide (GU4) derived from an antigen B (AgB) subunit was serologically compared with crude antigen (HCFA); immunopurified AgB and antigen 5 (Ag5), and two other synthetic peptides, for diagnosis of human cystic hydatidosis. GU4 was derived from the sequence of AgB/2, the novel AgB subunit described by us. The other two peptides: 65 (AgB mimotope) and 89-122 (Ag5 mimotope), were described by others. Antigens B and 5 showed higher diagnostic sensitivity than corresponding peptides. All sera reacting with peptides 89-122 and GU4 also reacted with 65. The latter provided three to four times higher sensitivity than the former two peptides, but 30% lower specificity. The diagnostic efficiency of AgB (82%) was higher than those of Ag5 (74%) and HCFA (71%). Interestingly, 89-122 only reacted with hydatid sera, some of which did not react with AgB. Considering positive those reacting with 89-122 or AgB, sensitivity increases from 77% (with AgB) to 82% (combined), while specificity is the same as with AgB (86%). Our results suggest that hydatid serology may be improved by: a) combining several defined antigens (including synthetic peptides), b) design of new E. granulosus-specific mimotopes, which react with the false negative sera (16/90; 18%).</text>
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                <text>Parasite Immunology v. 20, 1998. -- p. 51-61</text>
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                <text>Blackwell Science</text>
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              <elementText elementTextId="320213">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;&#13;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;</text>
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                <text>In this work we describe the presence of Fc-binding activity on the suckers and tegument of E. granulosus protoscoleces. A fraction (PSA-Fc+) from protoscolex somatic antigens was isolated by affinity chromatography on human Fc-&amp;gamma;1-Sepharose. Analysis by SDS-PAGE of PSA-Fc+ showed that it contained two major components. Using mouse F(ab&amp;prime;)2-human Fc chimaeric monoclonal antibodies we verified that PSA-Fc+ bound mainly to human Fc-&amp;gamma;1 and Fc-&amp;gamma;3 isotypes. In addition, one of the components of PSA-Fc+ showed proteolytic activity. Both, Fc-binding and proteolytic activities localized on the protoscolex surface, may play a relevant role in the host-parasite interaction.</text>
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                <text>&lt;strong&gt; Baz, A&lt;/strong&gt;</text>
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                <text>&lt;strong&gt; Carol, H&lt;/strong&gt;</text>
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                <text>Parasite Immunology v. 20, , 1998. -- p. 399-404</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>&lt;strong&gt;Competition study for the base hydrolysis of trans-[Co(NH3)4(NH2CH3)X]n+complexes.&lt;/strong&gt;</text>
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                <text>Nitrite ion competition has been measured for the base hydrolysis reaction of rans-Co(N-H3)4(NH2CH3)Xn+ ions (X = Cl&amp;minus;, Br&amp;minus;, NO3&amp;minus; and SO42&amp;minus;) in 1.0 M NaNO2 at 25&amp;deg;C. Both O- and N- bonded Co(NH3)4(NH2CH3)NO22+ are formed. Subsequently the Co(NH3)4(NH2CH3)ONO2+ isomer rearranges in OH&amp;minus; to give the thermodynamically more stable Co(NH3)4(NH2CH3)NO22+ ion. The total NO2&amp;minus; captured shows a slight dependence on the overall charge of the complex and on the nature of X (R = 2.2, 2.1, 3.6 and 1.2 &amp;plusmn; 0.5% for X = Cl&amp;minus;, Br&amp;minus;, NO3&amp;minus;, NO3&amp;minus;, and SO42&amp;minus;, respectively). These results differ from those observed with complexes of the type Co(NH3)5Xn+ and Co(NH2CH3)5Xn+ where X includes a variety of anions.</text>
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                <text>&lt;strong&gt;Benzo, Fabi&amp;aacute;n&lt;/strong&gt;</text>
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                <text>&lt;a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_cvuy/exportador/ExportarPdf?hash=6cd5b3d5a3031e37ab2fcbbcf9590d9d"&gt;&lt;strong&gt;Mendoza, Carolina&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>&lt;strong&gt;Queirolo, Marcelo&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Sienra, Beatriz&lt;/strong&gt;</text>
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                <text>Polyhedron v. 17, no. 13-14, 1998. -- p. 2295-2299</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>Fungal isolates are useful for wood biopulping, resulting in savings in energy and chemicals. They are also important for the bioconversion of agriculture and forestry products such as improving animal feed, release of simple sugars for ethanol production, and treatment of residual effluents. - Wood from Eucalyptus spp. is being increasingly used for pulp and paper production in South America, where Brazil and Uruguay are the main Kraft pulp producers from Eucalyptus spp. In Uruguay alone, for example, nearly 50% (two hundred thousand hectares) of the forested area designated for industrial purposes consists of Eucalyptus species, mainly E. globulus and E. grandis, with an estimated annual increase of 2.9% for the past 30 years. The screening of native strains of white-rot fungi, with the ability to degrade E. grandis and E. globulus woods, is therefore of particular value. The aim of this work was to test the preferential delignification of E. grandis and E. globulus by 42 native white-rot fungal strains isolated in Argentina, Brazil and Uruguay.</text>
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                <text>&lt;strong&gt;Speranza, M&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Sineriz, F&lt;/strong&gt;</text>
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                <text>Material und Organismen v. 32, no. 3, 1998. -- p. 227-233</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>&lt;strong&gt;Changes in the voltammetric response of titanium electrodes caused by potential programmes and illumination.&lt;/strong&gt;</text>
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                <text>ELECTRODOS DE TITANIO</text>
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                <text>The electrochemical behaviour of polycrystalline Ti electrodes was studied in dilute aqueous alkaline solutions at 25 &amp;deg;C. Fast repetitive potential programmes (triangular and square waves) were applied to Ti electrodes to grow different O-containing films. The growth efficiency of Ti oxides principally depends on the upper potential limit of the corresponding program. Potentiostatic preillumination of Ti electrodes produces surface modifications that can be followed by cyclic voltammetry. Two types of photoactive surface sites are evidenced; one coinciding with the potential window of the photogenerated molecular oxygen electroreduction reaction, and the other resulting from the electroreduction of photoformed oxygenated radicals. The application of both potential programmes and illumination can change the electrochemical properties of Ti surfaces in alkaline solutions.</text>
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                <text>&lt;strong&gt;Bonilla, S.H&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Zinola S&amp;aacute;nchez, Carlos Fernando&lt;/strong&gt;</text>
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                <text>Electrochimica acta v. 43, no. 3/4, 1998. -- p. 423</text>
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            <description>Information about rights held in and over the resource</description>
            <elementTextContainer>
              <elementText elementTextId="320955">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>&lt;strong&gt;Spontaneous and base catalyzed nitrito to nitro linkage isomerization of trans-[Co(NH)3)4(NH2CH3)ONO]2+complex.&lt;/strong&gt;</text>
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                <text>The nitrito ion trans-[Co(NH3)4(NH2CH3)ONO]2 rearranges to its nitro form trans-[Co(NH3)4 (NH2CH3)NO2]2 in aqueous base according to the rate law kobs=ks+kbc[OH&amp;minus;]. At &amp;mu;=1.0 M, 25&amp;deg;C ks=1.01&amp;times;10&amp;minus;4 s&amp;minus;1; &amp;Delta;Hs &amp;lowast;=98 kJ mol&amp;minus;1; &amp;Delta;Ss &amp;lowast;=7 JK&amp;minus;1; kbc=3.3&amp;times;10&amp;minus;2 M&amp;minus;1 s&amp;minus;1; &amp;Delta;H&amp;lowast; bc=121 kJ mol&amp;minus;1; &amp;Delta;S&amp;lowast; bc=132 JK&amp;minus;1 mol&amp;minus;1. The base-catalyzed isomerization proceeds enterely via an intramolecular conjugate-base mechanism. The reaction is fully retentive and trans-[Co(NH3)4(NH2CH3)NO2]2 is the only species that could be detected by 1H nmr after the reaction was completed. The results are discussed in the light of the reaction mechanisms proposed so far and in relation to the normal substitution chemistry of pentaamminecobalt(III) complexes.</text>
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                <text>Polyhedron v. 17, no. 20, 1998. -- p. 3551-3556</text>
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&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
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                <text>The present work describes a new experimental model of secondary infection which allows, through the recovery of the parasite together with its localin vivoenvironment, examination of the local nonadaptive immune response of the infected host and the differentiation of the parasite from protoscoleces to cysts. In this model we administered protoscoleces within silicone diffusion chambers, previously implanted into the peritoneal cavities of mice. The process of designing the model involved, first, determination of the optimal time postimplantation to infect the mice and, second, evaluation of the parasite's ability to establish infection within the chambers. The optimal time for infection was considered to be after the inflammation caused by implantation of the chambers had subsided. Our results showed that by day 20 postsurgery, three parameters used as indications of inflammation (complement C3, serum amyloid P protein, and polymorphonuclear cells in the peritoneum and in the chamber contents) had reverted to their normal levels. In our study of parasite differentiation, we found that 2&amp;ndash;3% of the total number of parasites inoculated into the chambers were recovered as viable cysts after 100 days. Throughout the infection period, the population of parasites recovered was heterogeneous; certain parasite morphologies that have not been described previously were observed. In conclusion, the use of intraperitoneal diffusion chambers offers a potential tool for investigating thein vivodifferentiation process of secondary cysts ofEchinococcus granulosusin mice and the early local interactions between host and parasite during this process.</text>
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&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>A new approach for the control and interruption of enzymatic reactions via selective enzyme immobilization has been developed. The technique was exemplified by the use of three model enzymes with the corresponding macromolecular substrates: alpha-amylase/starch, trypsin/ insoluble collagen, and alkaline phosphatase/plasmid DNA. Prior to incubation with its substrate, each enzyme was provided with de novo thiol-groups by a two-step reaction involving N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP) and DTT. The chemical modification was achieved such that at least 80% of the native enzyme activity was preserved in all cases. In order to interrupt rapidly the reactions in which the enzymes were used, the modified enzyme was immobilized by reaction via its thiol groups on a thiolsulfinate-agarose derivative. The gel-bound enzyme could then be easily removed from unreacted substrate and product by filtration or centrifugation. Comparative studies showed that the immobilized enzymes had much lower activities in the reactions studied than the corresponding soluble ones. The potential for enzyme reuse was also demonstrated with the alpha-amylase derivatives, which were quantitatively released and eluted in fully active form from the agarose. We have shown that it is possible to achieve practically complete enzyme immobilization in short times and thus to control the progress of the reactions. Because of its simplicity and high efficiency, this approach may represent an interesting alternative for biotechnological processes involving macromolecular or solid substrates</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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