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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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                <text>Echinococcus granulosus, the aetiological agent of cystic hydatid disease, exists as a series of strains or genotypes which differ in biological features. Pig strain (G7 genotype) has been shown to differ from sheep strain (G1 genotype) in phenotypical characters such as intermediate host range, geographical distribution and rate of development of the adult worm. Since in vivo studies of different parasite genotypes can provide insights into host&amp;ndash;parasite relationship we analysed for the first time the behaviour of E. granulosus G7 genotype protoscoleces in the murine experimental model. Our results show that G7 protoscoleces were unable to establish a regular infection in mice in contrast to G1 protoscoleces which developed intraperitoneal hydatid cysts. This inability was observed in co-infection experiments, i.e. even in the presence of a controlled immune response that allows G1 genotype protoscoleces establishment. In addition, the implantation of in vitro obtained E. granulosus G7 genotype microcysts resulted in a low percentage of hydatid cysts establishment. These results show a difference in the biological ability of both E. granulosus strains to develop secondary hydatid cysts in mice. We suggest that the comparison of infective and non infective genotypes of E. granulosus in the experimental host can be regarded as a new model to study the mechanisms of infection of Echinococcus spp. This knowledge could provide helpful information for the development of therapies, drugs and/or vaccines against cystic hydatid disease.</text>
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&lt;p style="margin-bottom: 0cm;"&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;&#13;
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                <text>The Piperaceae family is represented by herbaceous plants, shrubs, and, more rarely, trees. Among the aromatic varieties of this genus, Piper hispidinervum C. DC. stands out. In this work, volatile and non‐volatile extracts from P. hispidinervum were obtained through the application of sequential extraction methods. The results of steam distillation experiments indicated that optimal conditions were achieved when the extractions were conducted at 101&amp;thinsp;kPa and an initial bed porosity of 0.91. Chemical analysis showed that safrole is the major component present in the essential oil. NMR analysis revealed the presence of 6‐methoxy eugenol for P. hispidinervum extracts obtained by supercritical fluid extraction using water as co‐solvent at 20&amp;thinsp;MPa and 313.15&amp;thinsp;K. Mathematical models adjusted well to the experimental data in all conditions investigated. The methodology proposed represents an improvement in the processing of natural products, since the same material is submitted to different technologies, obtaining different compounds with distinct properties</text>
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                <text>&lt;strong&gt;Rossa, Guilherme E.&lt;/strong&gt;</text>
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                <text>The Canadian Journal of Chemical Engineering v. 96, 2018. -- p. 756-762</text>
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&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>To determine the factor structure of the Latin American &amp;amp; Caribbean Household Food Security Scale (ELCSA) and to study the influence of sociodemographic characteristics on each of the identified dimensions in Montevideo, Uruguay. Cross-sectional survey with a representative sample of urban households. Household food insecurity was measured using the ELCSA. The percentage of respondents who gave affirmative responses for each of the items of the ELCSA was determined. Exploratory factor analysis was carried out to determine the ELCSA&amp;rsquo;s factor structure. A probit model was used to determine the impact of some individual and household sociodemographic characteristics on the identified dimensions of food insecurity. Metropolitan area centred on Montevideo, the capital city of Uruguay, April&amp;ndash;September 2014. Adults aged between 18 and 93 years (n 742). The percentage of affirmative responses to the items of the ELCSA ranged from 4&amp;middot;4 to 31&amp;middot;7 %. Two factors were identified in the exploratory factor analysis performed on data from households without children under 18 years old, whereas three factors were identified for households with children. The identified factors were associated with different severity levels of food insecurity. Likelihood of experiencing different levels of food insecurity was affected by individual characteristics of the respondent as well as characteristics of the household. The influence of sociodemographic variables varied among the ELCSA dimensions. Household income had the largest influence on all dimensions, which indicates a strong relationship between income and food insecurity.</text>
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                <text>Public Health Nutrition&amp;nbsp; v. 20, no. 4, 2017. -- p. 620-629</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Sub-lethal effects of the consumption of Eupatorium buniifolium essential oil in honeybees&lt;/strong&gt;</text>
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                <text>When developing new products to be used in honeybee colonies, further than acute toxicity, it is imperative to perform an assessment of risks, including various sublethal effects. The long-term sublethal effects of xenobiotics on honeybees, more specifically of acaricides used in honeybee hives, have been scarcely studied, particularly so in the case of essential oils and their components. In this work, chronic effects of the ingestion of Eupatorium buniifolium (Asteraceae) essential oil were studied on nurse honeybees using laboratory assays. Survival, food consumption, and the effect on the composition of cuticular hydrocarbons (CHC) were assessed. CHC were chosen due to their key role as pheromones involved in honeybee social recognition. While food consumption and survival were not affected by the consumption of the essential oil, CHC amounts and profiles showed dose-dependent changes. All groups of CHC (linear and branched alkanes, alkenes and alkadienes) were altered when honeybees were fed with the highest essential oil dose tested (6000 ppm). The compounds that significantly varied include n-docosane, n-tricosane, n-tetracosane, ntriacontane, n-tritriacontane, 9-tricosene, 7-pentacosene, 9-pentacosene, 9-heptacosene, tritriacontene, pentacosadiene, hentriacontadiene, tritriacontadiene and all methyl alkanes. All of them but pentacosadiene were up-regulated. On the other hand, CHC profiles were similar in healthy and Nosema-infected honeybees when diets included the essential oil at 300 and 3000 ppm. Our results show that the ingestion of an essential oil can impact CHC and that the effect is dose-dependent. Changes in CHC could affect the signaling process mediated by these pheromonal compounds. To our knowledge this is the first report of changes in honeybee cuticular hydrocarbons as a result of essential oil ingestion&#13;
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                <text>&lt;strong&gt;Garrido, Paula Melisa&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Cuniolo, Antonella&lt;/strong&gt;</text>
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                <text>Plos One v. 15, 2020. --p. 1-19.--e0241666&#13;
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                <text>&lt;strong&gt;When a tritrophic interaction goes wrong to the third level:&amp;nbsp;xanthoxylin from trees causes the honeybee larval mortality in colonies affected by the river disease&lt;/strong&gt;</text>
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                <text>&lt;p&gt;The &amp;ldquo;River Disease&amp;rdquo; (RD), a disorder impacting honeybee colonies located close to waterways with abundant riparian&lt;br /&gt;vegetation (including Sebastiania schottiana, Euphorbiaceae), kills newly hatched larvae. Forager bees from RD-affected&lt;br /&gt;colonies collect honeydew excretions from Epormenis cestri (Hemiptera: Flatidae), a planthopper feeding on trees of S.&lt;br /&gt;schottiana. First-instar honeybee larvae fed with this honeydew died. Thus, we postulated that the nectars of RD-affected&lt;br /&gt;colonies had a natural toxin coming from either E. cestri or S. schottiana. An untargeted metabolomics characterization of&lt;br /&gt;fresh nectars extracts from colonies with and without RD allowed to pinpoint xanthoxylin as one of the chemicals present in higher amounts in nectar from RD-affected colonies than in nectars from healthy colonies. Besides, xanthoxylin was also&lt;br /&gt;found in the aerial parts of S. schottiana and the honeydew excreted by E. cestri feeding on this tree. A larva feeding assay&lt;br /&gt;where xanthoxylin-enriched diets were offered to 1st&lt;br /&gt;instar larvae showed that larvae died in the same proportion as larvae did when offered enriched diets with nectars from RD-colonies. These findings demonstrate that a xenobiotic can mimic the RD syndrome in honeybee larvae and provide evidence of an interspecific flow of xanthoxylin among three trophic levels. Further, our results give information that can be considered when implementing measures to control this honeybee disease.&lt;/p&gt;</text>
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                <text>Journal of Chemical Ecology,&amp;nbsp;v. 47, 2021. -- pp. 777&amp;ndash;787.</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt;&amp;nbsp;de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt;&amp;nbsp;La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;span id="docs-internal-guid-a96c0824-7fff-0b26-3d6b-6daf8a7a8502"&gt;&lt;span&gt;10.1007/s10886-021-01296-5&amp;nbsp; &lt;/span&gt;&lt;/span&gt;</text>
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                <text>&lt;strong&gt;Streamlined method for parallel identification of single domain antibodies to membrane receptors on whole cells&lt;/strong&gt;</text>
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                <text>Background&lt;br /&gt;&lt;br /&gt;Owing to their minimal size, high production yield, versatility and robustness, the recombinant variable domains (nanobodies) of camelid single chain antibodies are valued affinity reagents for research, diagnostic, and therapeutic applications. While their preparation against purified antigens is straightforward, the generation of nanobodies to difficult targets such as multi-pass or complex membrane cell receptors remains challenging. Here we devised a platform for high throughput identification of nanobodies to cell receptor based on the use of a biotin handle.&lt;br /&gt;Methods&lt;br /&gt;&lt;br /&gt;Using a biotin-acceptor peptide tag, the in vivo biotinylation of nanobodies in 96 well culture blocks was optimized allowing their parallel analysis by flow cytometry and ELISA, and their direct use for pull-down/MS target identification.&lt;br /&gt;Results&lt;br /&gt;&lt;br /&gt;The potential of this strategy was demonstrated by the selection and characterization of panels of nanobodies to Mac-1 (CD11b/CD18), MHC II and the mouse Ly-5 leukocyte common antigen (CD45) receptors, from a VHH library obtained from a llama immunized with mouse bone marrow derived dendritic cells. By on and off switching of the addition of biotin, the method also allowed the epitope binning of the selected Nbs directly on cells.&lt;br /&gt;Conclusions&lt;br /&gt;&lt;br /&gt;This strategy streamlines the selection of potent nanobodies to complex antigens, and the selected nanobodies constitute ready-to-use biotinylated reagents.&lt;br /&gt;General significance&lt;br /&gt;&lt;br /&gt;This method will accelerate the discovery of nanobodies to cell membrane receptors which comprise the largest group of drug and analytical targets.&lt;br /&gt;&lt;br /&gt;</text>
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                <text>&lt;strong&gt;Rossotti, Mart&amp;iacute;n&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Tabares, Sof&amp;iacute;a&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Alfaya, Luc&amp;iacute;a&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Leizagoyen, Carmen&lt;/strong&gt;</text>
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                <text>Biochimica et Biophysica Acta v. 1850, no. 7, 2015. -- p. 1397-1404</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt; &lt;br /&gt; (Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt; &lt;br /&gt; (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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                <text>&lt;strong&gt;Increasing the potency of neutralizing single-domain antibodies by functionalization with a CD11b/CD18 binding domain&lt;/strong&gt;</text>
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                <text>ENSAYO INMUNO ENZIMATICO</text>
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                <text>INMUNOTERAPIA</text>
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                <text>2015</text>
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                <text>Recombinant single domain antibodies (nanobodies) constitute an attractive alternative for the production of neutralizing therapeutic agents. Their small size warrants rapid bioavailability and fast penetration to sites of toxin uptake, but also rapid renal clearance, which negatively affects their performance. In this work, we present a new strategy to drastically improve the neutralizing potency of single domain antibodies based on their fusion to a second nanobody specific for the complement receptor CD11b/CD18 (Mac-1). These bispecific antibodies retain a small size (~30 kDa), but acquire effector functions that promote the elimination of the toxin-immunocomplexes. The principle was demonstrated in a mouse model of lethal toxicity with tetanus toxin. Three anti-tetanus toxin nanobodies were selected and characterized in terms of overlapping epitopes and inhibition of toxin binding to neuron gangliosides. Bispecific constructs of the most promising monodomain antibodies were built using anti Mac-1, CD45 and MHC II nanobodies. When co-administered with the toxin, all bispecific antibodies showed higher toxin-neutralizing capacity than the monomeric ones, but only their fusion to the anti-endocytic receptor Mac-1 nanobody allowed the mice to survive a 10-fold lethal dose. In a model of delayed neutralization of the toxin, the anti- Mac-1 bispecific antibodies outperformed a sheep anti-toxin polyclonal IgG that had shown similar neutralization potency in the co-administration experiments. This strategy should have widespread application in the development of nanobody-based neutralizing therapeutics, which can be produced economically and more safely than conventional antisera.</text>
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                <text>&lt;strong&gt;Rossotti, Mart&amp;iacute;n&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Guarnaschelli, Julio&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Yim, Lucia&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Camacho, Ximena&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Fern&amp;aacute;ndez Silveira, Marcelo.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Cabral, Pablo&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Leizagoyen, Carmen&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Chabalgoity Jose A.&lt;/strong&gt;</text>
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                <text>MAbs v. 7, no. 5, 2015. -- p. 820-828</text>
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                <text>Taylor &amp;amp; Francis Group</text>
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            <name>Rights</name>
            <description>Information about rights held in and over the resource</description>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Ingl&amp;eacute;s</text>
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                <text>DOI: 10.1080/19420862.2015.1068491</text>
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        <name>Nanocuerpos</name>
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                  <text>Versiones electrónicas de las tesis de doctorado, maestría y trabajos de diploma de la Facultad de Química, Universidad de la República (UdelaR).&#13;
Incluye únicamente trabajos hasta el año 2023. Los trabajos posteriores se encuentran disponibles en el Repositorio Colibrí.</text>
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                  <text>Departamento de Documentación y Biblioteca</text>
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                  <text>RIQUIM</text>
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                <text>&lt;strong&gt;Plataformas para acelerar el descubrimento de nanobodies contra blancos de inter&amp;eacute;s biom&amp;eacute;dico y sus aplicaciones&lt;/strong&gt;</text>
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                <text>INMUNOLOGIA APLICADA</text>
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                <text>ANTICUERPOS RECONBINATIVOS</text>
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                <text>URUGUAY</text>
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                <text>TESIS DE DOCTORADO</text>
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                <text>Adem&amp;aacute;s de los anticuerpos convencionales, los cam&amp;eacute;lidos poseen anticuerpos carentes de cadena liviana en los que un &amp;uacute;nico dominio forma el sitio de uni&amp;oacute;n al ant&amp;iacute;geno. Este dominio, denominado Nanobody o VHH, es uno de los fragmentos de reconocimiento de ant&amp;iacute;geno m&amp;aacute;s peque&amp;ntilde;os conocidos en la naturaleza (~15 kDa) y se caracterizan por su alta estabilidad, solubilidad y especificidad. Estas caracter&amp;iacute;sticas &amp;uacute;nicas de los VHHs junto a su facilidad de manipulaci&amp;oacute;n mediante biolog&amp;iacute;a molecular convierten a los VHH en una prometedora alternativa a los anticuerpos convencionales o fragmentos de anticuerpo para numerosas aplicaciones biotecnol&amp;oacute;gicas. Este trabajo de tesis tuvo como horizonte establecer tecnolog&amp;iacute;a local para la generaci&amp;oacute;n de bibliotecas de nanobodies en fagos filamentosos a partir de linfocitos B de llamas (Lama glama). Asimismo se desarrollaron metodolog&amp;iacute;as que facilitan la selecci&amp;oacute;n y caracterizaci&amp;oacute;n de los nanobodies de inter&amp;eacute;s contra una variedad de ant&amp;iacute;genos que van desde peque&amp;ntilde;as mol&amp;eacute;culas hasta complejas estructuras celulares. La funcionalidad de los nanobodies seleccionados se demostr&amp;oacute; en diferentes aplicaciones biotecnol&amp;oacute;gicas. En la primera parte del trabajo se presenta el desarrollo de un vector de alto nivel de expresi&amp;oacute;n y un sistema de biotinilacion in vivo de los nanobodies que facilita el proceso de screening y caracterizaci&amp;oacute;n paralela de cientos de clones en forma sistematizada y r&amp;aacute;pida. El sistema tiene gran versatilidad y facilita el estudio de la reactividad de los nanobodies mediante citometr&amp;iacute;a de flujo, ELISA, microscop&amp;iacute;a, inmunoprecipitaci&amp;oacute;n, etc. Como primer aplicaci&amp;oacute;n de este sistema se desarroll&amp;oacute; un m&amp;eacute;todo de ranqueo de afinidad relativa por el ant&amp;iacute;geno de la poblaci&amp;oacute;n de nanobodies aislados en la etapa de selecci&amp;oacute;n inicial (panning) y un sistema que permite la b&amp;uacute;squeda de pares de nanobodies para la detecci&amp;oacute;n del ant&amp;iacute;geno en inmunoensayos tipo s&amp;aacute;ndwich, lo que permiti&amp;oacute; el desarrollo de un ELISA ultrasensible para la cuantificaci&amp;oacute;n de la epoxi-hidrolasa humana. 2 En la segunda parte del trabajo se describe la preparaci&amp;oacute;n de una biblioteca de nanobodies contra c&amp;eacute;lulas dendr&amp;iacute;ticas murinas, y la adaptaci&amp;oacute;n de la plataforma de descubrimiento de nanobodies para aislar anticuerpos contra receptores celulares nativos utilizando c&amp;eacute;lulas enteras para las etapas de aislamiento y caracterizaci&amp;oacute;n. De esta forma se aislaron anticuerpos contra el componente CD11b del receptor Mac-1, la regi&amp;oacute;n no polim&amp;oacute;rfica del MHC-II y contra el receptor CD45, constituyendo un desarrollo de gran relevancia debido a que, a diferencia de los anticuerpos convencionales, los nanobodies pierden dr&amp;aacute;sticamente la reactividad contra su ant&amp;iacute;geno ante peque&amp;ntilde;os cambios conformacionales. Debido a eso los nanobodies seleccionados contra receptores recombinantes inmovilizados sobre poliestireno (placa de ELISA) muy raramente reaccionan en forma cruzada con el receptor nativo en las c&amp;eacute;lulas. A pesar de esto &amp;uacute;ltimo, y dada la importancia de dirigir la selecci&amp;oacute;n utilizando receptores recombinantes, se mostr&amp;oacute; que la cuidadosa elecci&amp;oacute;n de la forma de inmovilizaci&amp;oacute;n del receptor recombinante, s&amp;iacute; permite aislar nanobodies contra receptores nativos. Esto fue demostrado seleccionando nanobodies contra la cadena CD11c del receptor CDR4 que es un marcador de c&amp;eacute;lulas dendr&amp;iacute;ticas. Por &amp;uacute;ltimo se realiz&amp;oacute; una prueba de concepto sobre la utilidad de los nanobodies como dominios de funcionalizaci&amp;oacute;n, en este caso para conferir funciones efectoras a nanobodies con capacidad de neutralizar la toxina tet&amp;aacute;nica. Una vez aislados los nanobodies anti-toxina, se prepararon anticuerpos biespec&amp;iacute;ficos fusion&amp;aacute;ndolos a los nanobodies anti- CD11b, MHCII y CD45, y se estudi&amp;oacute; su poder de neutralizaci&amp;oacute;n y biodistribuci&amp;oacute;n in vivo, encontrando un dram&amp;aacute;tico incremento del poder neutralizante cuando los anticuerpos neutralizantes se funcionalizaron con el nanobody anti-CD11b (que reacciona con un receptor endoc&amp;iacute;tico), pero no con el anti-MHC-II o anti-CD45.</text>
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                <text>&lt;strong&gt;Rossotti, Martin&lt;/strong&gt;</text>
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                <text>Montevideo : UelaR-Facultad de Qu&amp;iacute;mica</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Two different Echinococcus granulosus antigen B subunits (AgB8:1 and AgB8:2) were characterized and the structure of the genes encoding these two proteins were compared. DNA sequences were expressed in Escherichia coli and the antigens&amp;rsquo; diagnostic value was then assessed. The genomic sequence of AgB8:1 has a 92 bp intron in the position corresponding to amino acid 16; the AgB8:2 genomic sequence presents a 68 bp intron in the position corresponding to amino acid 20. Both introns are located between the putative N-terminal hydrophobic sequence and the secreted peptide. A comparison between the AgB8:1 and AgB8:2 nucleotide sequences showed a 53.5% identity among exons and a 50% identity between introns. According to the molecular diversity analysis, the elapsed time since both genes shared a common ancestor would be around 4.2�107 years. When the native AgB and the two recombinant antigens (rAgB8:1 and rAgB8:2) were tested in an anti-IgG ELISA, the sensitivity of the native antigen B was 77.41% and its specificity was 81.9%, while rAgB8:1 showed 54.84% of sensitivity and 80.17% of specificity and rAg138:2 had an 83.87% sensitivity and a 98.28% specificity. Statistical analysis confirms that rAgB8:2 has a better performance than rAgB8:1 and native AgB in ELISA</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Rovira de Miguel, Avelina&lt;/strong&gt;</text>
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                <text>Anales de la Asociaci&amp;oacute;n de Qu&amp;iacute;mica y Farmacia del Uruguay v. 46, n&amp;ordm;. 2, 1943. -- p. 36-40</text>
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                <text>Asociaci&amp;oacute;n de Qu&amp;iacute;mica y Farmacia (Uruguay)</text>
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            <name>Rights</name>
            <description>Information about rights held in and over the resource</description>
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              <elementText elementTextId="396208">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt; &lt;br /&gt; (Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt; &lt;br /&gt; (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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        <name>Fotometría</name>
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                <text>&lt;strong&gt;Effect of beef long-storage under different temperatures and vacuum-packaging conditions on meat quality, oxidation processes and microbial growth&lt;/strong&gt;</text>
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                <text>CARNE VACUNA</text>
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                <text>CALIDAD DE LA CARNE</text>
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                <text>OXIDACION</text>
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                <text>CONSERVACION DE LOS ALIMENTOS</text>
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                <text>BIBLIOGRAFIA NACIONAL QUIMICA</text>
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                <text>2023</text>
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                <text>BACKGROUND: The global beef market demands the meat industry to ensure product quality and safety in markets that are often very distant. The present study aimed to evaluate the effects of chilled (CH, 120 d) and chilled-then-frozen (CHF, 28 d + 92 d) storage conditions of beef vacuum packaged (VP) and vacuum packaged with antimicrobial (VPAM) on meat quality, oxidative status and microbial loads. Treatments resulted from the combination of storage condition and packaging type: VP + CH, VP + CHF, VPAM + CH and VPAM + CHF. RESULTS: Warner&amp;ndash;Bratzler shear force values decreased in all treatments after 28 d of chilling. Except for VP + CH, L* values (lightness) of meat color did not differ in each treatment as the storage time increased. Meat from VP + CH had greater a* values than CHF treatments on day 120 of storage. A consumer panel did not detect differences in tenderness, flavor and overall liking between VP and VPAM beef, but they preferred CHF steaks rather than CH beef. TBARS values did not differ between VP and VPAM and between CH and CHF at any time during the storage period. At the end of storage time, all treatments except VP + CHF presented a greater concentration of thiols than at 48 h post-mortem. On day 120 of storage, VP + CH had greater cat alase enzyme activity than CHF treatments while VP + CH and VP + CHF showed a greater superoxide dismutase activity than VPAM + CHF. Storage condition (CH or CHF) had a greater impact on microbial counts than the type of packaging. CONCLUSION: Freezing meat after an ageing period represents a suitable strategy to extend beef storage life without a detri mental impact on its quality.</text>
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                <text>&lt;strong&gt;Luzardo, Santiago&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Saadoun, Ali&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Cabrera, Mar&amp;iacute;a C.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Terevinto, Alejandra&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;de Souza, Guillermo&lt;/strong&gt;</text>
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                <text>Journal of the Science of Food and Agriculture, 2023.</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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                <text>Molecular dynamics simulations of the zinc finger domain of protein Zif268, in a complex with a high affinity DNA sequence, yields a globally stable system with small yet significant readjustments with persistence time of the order of 1.1ns. The results confirm the quality of the standard GROMOS87 force field with a corrected solvent-to-solute interaction that does not affect the water-water SPC interactions nor the intra-molecular cohesive forces. Specificity determinants are discussed. The simulations of DNA alone, with the same force field, showed the important role played by the solvent and the symmetry of the counterion distribution. (Tapia &amp;amp; Vel&amp;aacute;zquez, J. Am. Chem. Soc., 119, 5934, 1997) In the present work, this feature was retained when appropriate. The results for root mean square deviations and temperature B-factors illustrate the reliability of this approach. The structure of DNA is held by its interactions with the zinc finger protein. This behavior is not much affected by the slow whithering away of finger-1 from DNA. The factors contributing to the molecular stability found in GROMOS' potential energy function appear to be sufficient to yield stable fluctuation patterns when surrounding medium effects are properly included.</text>
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&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>&lt;p&gt;&lt;strong&gt;Molecular dynamics Simulation of a Zif268-DNA Complex in Water. Spatial Patterns and Fluctuations Sensed from a Nanosecond Trajector.&lt;/strong&gt;&lt;/p&gt;</text>
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                <text>In this paper, with the help of a nanosecond-long molecular dynamics trajectory, we show that a fully charged protein&amp;minus;DNA system (Zif268-DNA) in a water environment can be simulated with a general force field (GROMOS) if solvation and counterion effects are appropriately represented. A model exhibiting ionic-atmosphere effects on mobile counterions (Tapia, O.; Vel&amp;aacute;zquez, I. J. Am. Chem. Soc. 1997, 119, 5934&amp;minus;5938) was implemented. The root-mean-square deviations (rmsds) with respect to X-ray structure for the full complex, the protein, and the 12-base pair consensus sequence were 2.0, 1.95, and 1.35 &amp;Aring;, respectively, while the counterions displayed an rmsd from the initially equilibrated position of 1.2 &amp;Aring;. The mean-square fluctuation with respect to the average structure correlated with temperature factors for the protein and DNA; the agreement in trend is good. The results show that GROMOS87 force field with an appropriate representation of coion (ion atmosphere) effects on the counterions, and corrections for hydrophobicity default in the water to solute C12(Ow,Ow)1/2 parameter, previously used for proteins (Daura X.; Oliva, B.; Querol, E.; Avil&amp;eacute;s, F. X.; Tapia, O. Proteins 1996, 25, 89&amp;minus;103), are sufficient to obtain a fairly realistic simulation of this protein&amp;minus;DNA complex.</text>
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                <text>&lt;strong&gt;Association of blood lead levels with urinary F2-8&amp;alpha; isoprostane and 8-hydroxy-2-deoxy-guanosine concentrations in first-grade Uruguayan children&lt;/strong&gt;</text>
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                <text>xidative stress (OS) is a potential molecular mechanism for lead-induced toxicities, yet, we have limited understanding of the relation between low-level lead (Pb) exposure and OS, especially in children. This cross-sectional study examines the association between blood lead level (BLL) and two OS markers-urinary F2-8α isoprostane or isoprostane (a marker of lipid peroxidation) and 8-hydroxy-2-deoxy-Guanosine or 8-OH-dG (a marker of DNA damage) in 211 children, aged 5-8 years, from Montevideo, Uruguay. The role of dietary intakes of vitamin C and zinc in modifying the relation between BLL and OS was also examined. The mean (SD) BLL of the study children was 4.7 (2.2) µg/dL, with 30.2% children having BLL ≥5 µg/dL, the current reference level set by the US Centre for Disease Control for identifying, monitoring and management of children with elevated BLL. In covariate-adjusted analysis, there was a weak positive association between BLL and urinary isoprostane (adjusted for specific gravity) [β=0.09, p&lt;0.1]. No association was found between children's BLL and urinary 8-OH-dG. Interactions between dietary intakes of vitamin C or zinc and BLL on OS biomarkers were not consistent. However, when BLL and vitamin C or BLL and zinc were modeled together, BLL was independently associated with isoprostane concentration [β=0.10, p&lt;0.05] but vitamin C or zinc intake was not. These findings suggest that there may be a potential adverse effect of BLL on OS in children with low-level Pb exposure. There is a need to study the effects of Pb on other OS measures, as well as the role of OS in mediating low-level Pb toxicity on functional outcomes.</text>
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                <text>&lt;strong&gt;Kordas K.&lt;/strong&gt;</text>
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                <text>Environmental Research v. 140, 2015. --p. 127-135</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Improved glucoamylase immobilization onto calcined chicken bone particles&lt;/strong&gt;</text>
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                <text>Calcination was considered for the first time as an alternative to recover inorganic bone component from spent glucoamylase-bone derivatives. The subsequent adsorption of glucoamylase (GA) onto calcined bone particles was assessed. Adsorption capacity of the calcined matrix was found to be from 1.1- to 1.4-fold superior to that of non-calcined supports, and it was dependent on the applied load. Moreover, the expressed activity of GA derivatives on calcined matrix was, at least, 2-fold higher than that of biocatalysts onto non-calcined support. The optimization of the loading allowed the preparation of derivatives with 139 GA units per gram of support, which preserve 52% of the immobilized activity. Additionally, calcination of spent GA biocatalysts on calcined bone particles was performed, and adsorption of glucoamylase onto the bone particles calcined a second time was also found to be efficient. In addition to the improved catalytic properties, the half-life at 55&amp;deg;C of the GA biocatalysts on calcined bone was increased 1.7- fold in comparison with that of soluble GA and GA adsorbed onto non-calcined bone particles. Furthermore, the same cassava starch conversion can be achieved batchwise in a stirred-tank reactor using less insoluble biocatalyst, 37% of the GA-bone derivative, which represents an important saving for industrial applications.</text>
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                <text>&lt;strong&gt;Carpio, Cecilia&lt;/strong&gt;</text>
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                <text>&lt;a title="Curr&amp;iacute;culum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8dfa5283acaafc5c73dab5b61b04f9a0" target="_blank"&gt;&lt;strong&gt;Batista-Viera, F.&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>&lt;strong&gt;Ruales, Jenny&lt;/strong&gt;</text>
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                <text>Food and Bioprocess Technology v. 4, no. 7, 2011. -- p. 1186-1196</text>
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                <text>&lt;p&gt;&amp;nbsp;&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;&#13;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;</text>
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                <text>Synthesis and biological characterization of a series of a-tocopherol analogs with NO-releasing capacity are reported. The selected NO-donor moieties were nitrooxy and furoxan. All products were tested for their in vitro NO-releasing capacities, vasodilating properties, and antiplatelet activity. They were also capable of preventing LDL oxidation.</text>
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                <text>&lt;strong&gt;Batthyany, C.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Blanco, F.&lt;/strong&gt;</text>
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                <text>Bioorganic and Medicinal Chemistry v. 13, no. 20, 2005. -- p. 5787-5796</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
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&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora</text>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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                <text>&lt;strong&gt;Nitroalkenes : characterization, and effects of macrophage activation. Cap&amp;iacute;tulo 3&lt;/strong&gt;</text>
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                <text>ACIDOS GRASOS</text>
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                <text>Nitroalkenes derivatives of free as well as esterified unsaturated fatty acids are present in human plasma and tissue, representing novel pluripotent cell signaling mediators. Lipid nitration occurs in response to pro-inflammatory stimuli as an adaptative mechanism to downregulate inflammatory responses. This chapter first discusses the generation of nitroalkenes during macrophage activation following chemical and biological characterization. In particular, it describes procedures for (a) synthesizing and characterizing esterified (cholesteryl-nitrolinoleate, CLNO2) as well as free (nitroarachidonate, AANO2) nitroalkenes, (b) determining nitration of cholesteryl linoleic acid during macrophage activation by inflammatory stimuli, (c) examining the modulatory effects of nitroalkenes on the expression of inducible enzymes by activated macrophages, and (d) discussing the signaling pathways involved in nitroalkene-mediated anti-inflammatory actions.</text>
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                <text>&lt;strong&gt;Ferreira, Ana Mar&amp;iacute;a&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Trostchansky Vasconcellos, Andr&amp;eacute;s Ezequiel&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Ferrari, Mariana&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Souza, Jos&amp;eacute; M.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Rubbo, Homero&lt;/strong&gt;</text>
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                <text>Methods in Enzymology v. 441, 2008. -- p. 34-49</text>
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                <text>Elsevier</text>
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                <text>2008</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt; &lt;br /&gt; (Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt; &lt;br /&gt; (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>DOI: 10.1016/S0076-6879(08)01203-2</text>
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        <name>Nitroalkenos</name>
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