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                <text>The study aimed at investigating the influence of four variables related to processing technology and formulation on consumer&amp;rsquo;s choice of fruit juice, and to explore the moderating effect of food technology neophobia (FTN). A choice‐conjoint task was implemented considering four variables: processing technology, added sugar, preservatives and cold pressing. Sixteen pairs of labels were presented to 369 Brazilian consumers to that indicate which of the products they would purchase. Then, they answered the FTN scales and a socio‐demographic questionnaire. Data were analysed using a logit model with random parameters. Results showed that cold pressing, pressurisation and the claims &amp;lsquo;no added sugar&amp;rsquo; and &amp;lsquo;no preservatives&amp;rsquo; had a positive effect on consumers&amp;rsquo; choices, whereas the claim &amp;lsquo;not pressurised&amp;rsquo; had a negative effect. The FTN moderated the effect of the variables on participants' choices of fruit juices. The main difference between groups of consumers with different level of neophobia (low, medium and high) was related to the effect of information about juice processing.&#13;
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                <text>Abstract Qualitative and quantitative determination of volatile markers in aqueous based fragrances assumes ever-increasing importance, because of both the need for quality control and the safety-regulatory limitations introduced for several compounds. This study reports and critically discusses the results of applying new water-compatible ionic-liquid (IL) GC stationary phases, based on phosphonium and imidazolium derivative cations combined with trifluoromethanesulphonate (Watercol&amp;trade;) to the direct quantitative analysis of aqueous samples in the perfume field with GC&amp;ndash;MS. Narrow-bore columns of different lengths, especially prepared for this study, were adopted to minimize the amount of water reaching the MS detector after GC separation. All GC&amp;ndash;MS analysis steps were investigated, to achieve results compatible with quality control requirements for the volatiles of interest in this field, in terms of LODs, LOQs, and repeatability. Reliability of the GC&amp;ndash;MS results was demonstrated by determining volatile allergens in two commercial perfumes, as per EU regulations concerning no-declaration limits for leave-on (0.001%) and rinse-off (0.01%) cosmetic products.</text>
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                <text>An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30T) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30T was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12&amp;ndash;43&amp;thinsp;&amp;deg;C (optimum, 25&amp;ndash;30&amp;thinsp;&amp;deg;C), at pH 8.0&amp;ndash;12 (optimum, pH 9.5&amp;ndash;10) and at salinities of 0.5&amp;ndash;15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9&amp;thinsp;mol%. The predominant menaquinone was MK-7 and the major fatty acids (&amp;gt;10 %) comprised anteiso-C15 : 0, iso-C15 : 0, and anteiso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30T to the genus Bacillus and showed the highest similarities to Bacillus populi FJAT-45347T (96.4 %) and Bacillus aurantiacus K1-5T (96.2 %). Based on the data from the current polyphasic study, M30T represents a novel species of the genus Bacillus , for which the name Bacillus natronophilus sp. nov. is proposed. The type strain is M30T (=JCM 32118T=CGMCC 1.16739T=MCC 3010T).</text>
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                <text>International Journal of Systematic and Evolutionary Microbiology. v. 70, 2020. -- p. 562-568 .</text>
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                <text>Successful establishment of a parasite infection depends partially on the host intrinsic susceptibility to the pathogen. In cystic echinococcosis (CE), a zoonotic disease caused by the cestode parasite Echinococcus granulosus, the infection outcome in the murine model of secondary CE varies according to the mouse strain used. In this regard, intrinsic differences in susceptibility to the infection were previously reported for Balb/c and C57Bl/6 mice, being C57Bl/6 animals less permissive to secondary CE. Induction of parasite-specific antibodies has been suggested to play relevant roles in such susceptibility/resistance phenomena. Here, we report an in deep comparison of antibody responses induced in both mouse strains. Firstly, only C57Bl/6 mice were shown to induce specific-antibodies with efficient anti-parasite activities during early secondary CE. Then, through ImmunoTEM and Serological Proteome Analysis (SERPA), an evaluation of specific antibody responses targeting parasite tegumental antigens was performed. Both strategies showed that infected C57Bl/6 mice -unlike Balb/c animals- narrowed their IgG recognition repertoire against tegumental antigens, targeting fewer but potentially more relevant parasite components. In this sense, tegumental antigens recognition between Balb/c and C57Bl/6 mice, either by natural and/or induced antibodies, was analyzed through SERPA and MALDI-TOF/TOF studies. A total of 13 differentially recognized proteins (DRPs) uniquely targeted by antibodies from C57Bl/6 mice were successfully identified, wherein a subset of 7 DRPs were only recognized by infection-induced antibodies, suggesting their potential as natural protective antigens. In this regard, immunoinformatic analyses showed that such DRPs exhibited higher numbers of possible T cell epitopes towards the H-2-IAb haplotype, which is present in C57Bl/6 mice but absent in Balb/c animals. In summary, our results showed that the genetic predisposition to generate better T-dependent antibody responses against particular tegumental antigens might be a key factor influencing host susceptibility in the murine model of secondary CE.</text>
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                <text>&lt;strong&gt;Magnone, Javier&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt; (Por favor lea este aviso antes de abrir los documentos u objetos)&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho de autor sobre toda creaci&amp;oacute;n&lt;/strong&gt; literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006) ADVERTENCIA - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>DOI: 10.1016/j.imbio.2020.151916</text>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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                  <text>Departamento de Documentación y Biblioteca</text>
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              <text>Pdf</text>
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                <text>&lt;strong&gt;Are nutritional warnings more efficient than claims in shaping consumers&amp;rsquo; healthfulness perception?&lt;/strong&gt;</text>
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                <text>ALIMENTOS</text>
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                <text>ALIMENTOS-ETIQUETAS</text>
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                <text>ADVERTENCIAS</text>
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                <text>BIBLIOGRAFIA NACIONAL QUIMICA</text>
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                <text>2020</text>
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                <text>Background: Brazil is currently debating the implementation of front-of-package labels. This study tested if Warning labels (WLs) improved consumer understanding, perceptions, and purchase intentions compared to Traffic-Light labels (TLLs) in 1607 Brazilian adults. Methods: In this online, randomized controlled experiment participants saw images of 10 products and answered questions twice&amp;mdash;once in a no-label, control condition and then again in a randomly assigned label condition. The relative differences in responses between WLs and TLLs between control and label conditions were estimated using one-way ANOVAs or Chi-square tests. Results: Presenting WLs on products compared to TLLs helped participants: (i) improve their understanding of excess nutrient content (27.0% versus 8.2%, p &amp;lt; 0.001); (ii) improve their ability to identify the healthier product (24.6% versus 3.3%, p &amp;lt; 0.001); (iii) decrease perceptions of product healthfulness; and (iv) correctly identify healthier products (14.0% versus 6.9%, p &amp;lt; 0.001), relative to the control condition. With WLs, there was also an increase in the percentage of people: (v) expressing an intention to purchase the relatively healthier option (16.1% versus 9.8%, p &amp;lt; 0.001); and (vi) choosing not to buy either product (13.0% versus 2.9%, p &amp;lt; 0.001), relative to the control condition. The participants in the WL condition had significantly more favorable opinions of the labels compared to those in the TLL group. Conclusions: WLs would be more effective, compared to the TLL, at improving consumer food choices.</text>
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                <text>&lt;strong&gt;Nobrega, L&amp;iacute;a&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Ares, Gast&amp;oacute;n&lt;/strong&gt;</text>
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                <text>Food Quality and Preference v. 79, 2020. -- p. 1-10. --e103749</text>
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                <text>Potato common scab is a worldwide disease mainly caused by Streptomyces scabiei. It seriously affects potato crops by decreasing tuber quality. Essential oils (EO) are natural products with recognised antimicrobial properties. In this research, the antibacterial activities of thyme, oregano, suico and mint EO against S. scabiei were analysed. Infected tubers and soil samples were used for bacterial isolation; the obtained isolates were genetically identified. The chemical composition of the EO was determined by GC-MS. The broth microdilution method was used to analyse antibacterial properties of EO. Thirty-one bacterial isolates were obtained. The isolate chosen for antibacterial assays was morpho-physiologically and genetically identified as S. scabiei. Thyme EO was mainly composed of thymol and o-cymene; suico EO of dihydrotagetone, trans-tagetone and verbenone; oregano EO of trans-sabinene hydrate, thymol and ɣ-terpinene; and mint EO of menthone and menthol. All the EO tested were effective against S. scabiei, but thyme and suico EO were the most successful, with a minimum inhibitory concentration of 0.068 g&amp;middot;l-1 and 0.147 g&amp;middot;l-1 , respectively, and a minimum bactericidal concentration of 0.137 g&amp;middot;l-1 and 0.147 g&amp;middot;l-1 , respectively. Scanning electron microscopy showed similar damage caused by both thyme and suico EO to the bacterial envelope. Total phenolic content of EO was not related to their antibacterial activity. Thyme and suico EO are effective antibacterial agents against S. scabiei, impeding bacterial viability and disturbing the bacterial cell envelope. These EO are promising tools for control of potato common scab.</text>
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                <text>Plant Biology v. 22, no. 1, 2020. -- p.81-89</text>
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                <text>Chemical and sensory properties of Torront&amp;eacute;s Riojano sparkling wines, prepared using second fermentation with Saccharomyces strains EC1118, bayanus C12 and IFI473I, were explored. All sparkling wines showed high levels of several volatile ethyl esters and terpenes associated to fruity and floral aromas. The sensory profiles showed significant differences for the floral aroma descriptor among EC1118, bayanus C12 and IFI473I and for bubble persistence for strain bayanus C12. Our results suggest that the sensory properties of these sparkling wines could be dependent on the chemical and organoleptic properties of the base wine more than the yeast strain used for second fermentation.&#13;
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                <text>The Pechini-type sol-gel (PSG) process has been used for the preparation of doped oxides due to its capability to overcome most of the difficulties that frequently occur by using other producing methods. In this work we analyze the case of samples of pure and In-doped yttria (Y2O3) prepared by the PSG process. We experimentally characterize the synthesized samples by x-ray diffraction, micro-Raman spectroscopy, electrochemical impedance spectroscopy (EIS), and time-differential perturbed &amp;gamma;-&amp;gamma; angular correlation (PAC) spectroscopy, and we compare these results with those obtained starting from commercial oxide powders. We found that the PSG process can be used to successfully produce doped yttria in the cubic phase, with the impurities substitutionally located at the cationic sites of the structure. By the proposed PSG route, the inclusion of impurities does not affect the particle size nor the resistivity. However, when we compare the PSG samples with other samples produced from commercial powder, we found that the first have lower resistivities at grain interiors. On the other hand, PAC spectroscopy in 111In(&amp;rarr;111Cd)-doped yttria allows the study of the dynamic hyperfine interactions observed by the radioactive 111Cd impurity-probe, which can be used to &amp;ldquo;sense&amp;rdquo; the host electron availability near the impurities after the electron-capture decay of 111In. Differences between PAC spectra for PSG samples and the commercial powder suggest that the PSG process introduces additional donor defects into the yttria electronic structure, which is consistent with the lower resistivity observed in the PSG samples by EIS spectroscopy.</text>
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                <text>There are three basic steps to activated carbon fibers (ACF) manufacturing, from PAN fiber: oxidation/stabilization, carbonization and activation. Carbon material, specially ACF is a very attractive material to be used as supercapacitor electrode. The literature describes carbon material surface chemistry importance for supercapacitors application, mainly nitrogen groups by N-doping. Oxidation/stabilization is an important non-explored factor that influence the surface chemical functionality. This work describes the influence of oxidation/stabilization process on ACF production, from textile PAN fiber, and the non-doping nitrogen surface chemistry characteristic caused by the oxidation process design. Its textural, structural and surface was evaluated for supercapacitor electrode. The results show that the oxidation degree can be used as a mechanism of textural and surface chemistry control. The surface chemistry is the key of this work, different oxidation conditions can produce nitrogen compounds that help to increase specific capacitance. The tests showed an increase in capacitance higher than 100% in comparison to the standard oxidation.</text>
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                <text>&lt;strong&gt;Ab-initio approach to the stability and the structural, electronic and magnetic properties of the (001) Znfe2O4 surface terminations&lt;/strong&gt;</text>
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                <text>We present a Density Functional Theory (DFT) based study of the structural and magnetic properties of the (001) surface of the semiconducting oxide ZnFe2O4 (spinel structure). The calculations were performed using the DFT based ab initio plane wave and pseudopotential method as implemented in the Quantum Espresso code. The all electron Full-potential linearized-augmented-plane-wave method (FP-LAPW) was also employed to check the reproducibility of the plane wave method. In both calculations the DFT+U methodology was employed and different (001) surface terminations of ZnFe2O4 were studied. We find that the surface terminated in Zn is the stable one. For all the (001) surface terminations our calculations predict that the Zn-Fe cationic inversion (anti-sites), which are defects in bulk ZnFe2O4, becomes stable and an integral part of the surface. Also, a ferrimagnetic behavior is predicted for the case of anti-sites in the superficial layer. Our results for different properties of the surface of ZnFe2O4 are compared with those obtained in bulk samples and those reported in the literature.</text>
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                <text>Applied Surface Science. v. 499, 2020. -- p. 1-7.--e143859</text>
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                <text>The inclusion of sodium warnings on food packages has been proposed as a means to encourage population to reduce sodium intake. However, consumers who focus on today rather than tomorrow or consumers who downplay or underestimate risks might pay little attention to warnings. The aim of the present study was to explore whether time orientation and perceived risk of sodium consumption may moderate the influence of sodium warnings on food choices. An online study involving 498 Uruguayan participants was carried out. Participants evaluated pairs of bread packages differing in three 2-level variables (sodium warning, type of bread and brand) and were asked to indicate the one they would choose if they were in a supermarket. Then, they answered a scale on &amp;lsquo;consideration of future consequences&amp;rsquo; adapted to eating habits, and responded to statements measuring perceived risk of sodium consumption. Results revealed that sodium warnings had a significant effect on participants' choices. However, the efficacy of warnings was moderated by time orientation and risk perception. A focus on immediate consequences and thinking that the risk associated with sodium consumption can be compensated, decreased the efficacy of the sodium warning. These results provide experimental evidence of the potential of warnings to discourage consumption of products with high sodium content and suggest that communication campaigns accompanying the introduction of warnings should promote a future-oriented vision on eating habits, raise risk awareness and stress that the risks are not easily compensated.</text>
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                <text>Appetite. v. 147, 2020. -- p. 1-7.--e104562</text>
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                <text>The NS2B-NS3 protease has been identified as an attractive target for drug development against Zika virus (ZIKV) and combined drug repurposing and structure-based virtual screening has improved the development of antiviral drugs. In this study, we performed a structure-based virtual screening of 1861 Food and Administration (FDA) approved drugs available in DrugBank by the selection and docking validation of crystal structure of ZIKV NS2B-NS3 protease (PDB ID 5H4I) using Glide and DOCK 6 software. The antihistaminic chlorcyclizine (Grid score &amp;minus;24.8 kcal/mol) exhibited the most promising interaction with NS2B-NS3 protease in comparison to crystallography ligand (Grid score &amp;minus;15.6 kcal/mol) by interaction to Tyr161 by hydrophobic interactions in the binding site of NS2B-NS3 which is recognized as an important amino acid in substrate molecular recognition. Cytotoxicity and global antiviral activity assay in Vero cells by MTT method showed that chlorcyclizine reduced the ZIKV induced cytopathic effect (EC50 of 69.0 &amp;plusmn; 7.3 &amp;mu;M and SI = 1.9), and explicit molecular dynamics simulations implemented on a NAMD program indicated great stability of chlorcyclizine in protease binding site, suggesting the repurposing of chlorcyclizine as a promising finding in anti-ZIKV drug development.</text>
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                <text>Journal of Chemical Information and Modeling. v. 60, 2020. -- p.731-737</text>
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                <text>On-resin intramolecular native chemical ligation (NCL) assisted by N-ethylcysteine using Fmoc/SPPS to obtain cyclic peptides is described. N-terminal cysteine-containing peptides were subjected to NCL conditions leading to cyclization&amp;ndash;cleavage reactions and consecutive S &amp;rarr; N shift, rendering cyclic peptides in good yields and purities. The compounds were evaluated against P. falciparum 3D7.</text>
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                <text>Many reports state the potential hazards of microplastics (MPs) and their implications to wildlife and human health. The presence of MP in the aquatic environment is related to several origins but particularly associated to their occurrence in wastewater effluents. The determination of MP in these complex samples is a challenge. Current analytical procedures for MP monitoring are based on separation and counting by visual observation or mediated with some type of microscopy with further identification by techniques such as Raman or Fourier-transform infrared (FTIR) spectroscopy. In this work, a simple alternative for the separation, counting and identification of MP in wastewater samples is reported. The presented sample preparation technique with further polarized light optical microscopy (PLOM) observation positively identified the vast majority of MP particles occurring in wastewater samples of Montevideo, Uruguay, in the 70&amp;ndash;600 &amp;mu;m range. MPs with different shapes and chemical composition were identified by PLOM and confirmed by confocal Raman microscopy. Rapid identification of polyethylene (PE), polypropylene (PP) and polyethylene terephthalate (PET) were evidenced. A major limitation was found in the identification of MP from non-birefringent polymers such as PVC (polyvinylchloride). The proposed procedure for MP analysis in wastewater is easy to be implemented at any analytical laboratory. A pilot monitoring of Montevideo WWTP effluents was carried out over 3-month period identifying MP from different chemical identities in the range 5.3&amp;ndash;8.2&amp;thinsp;&amp;times;&amp;thinsp;103 MP items/m3.</text>
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                <text>&lt;strong&gt;Sierra, Ignacio&lt;/strong&gt;</text>
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                <text>Environmental Science and Pollution Research v.27, 2020. -- p.7409-7419</text>
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                <text>&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt; (Por favor lea este aviso antes de abrir los documentos u objetos) &lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho de autor sobre toda creaci&amp;oacute;n&lt;/strong&gt; literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006) ADVERTENCIA - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>&lt;strong&gt;High-throughput and high peak capacity narrow-bore parallel segmented flow 2 column strategy for the liquid chromatography - tandem mass spectrometry 3 analysis of organic contaminants in water&lt;/strong&gt;</text>
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                <text>This study highlights the development of a high peak capacity, high-throughput (HTP) approach for a target list of 62 organic contaminants in an environmental river water matrix on a HPLC conventional 400 bar system. Key separation metrics were evaluated: (a) peak capacity, (b) total analysis time and (c) mobile phase consumption. An average peak width of 0.10 min with a total analysis time (inclusive of the column wash and re-equilibration) of 10 minutes revealed the increased productivity and performance of the parallel segmented flow (PSF) column technology HPLC-MS strategy, comparable to UHPLC peak widths and analysis times, achieved at a significantly lower backpressure (&lt;400 bar). The operation of the PSF in a narrow-bore scale format (internal diameter of 2.1 mm) resulted in a conservative HPLC scale total mobile phase consumption of 15 mL for the column separation; only 5.5 mL of this volume was exposed to the ion source per injection. Mobile phase consumption was higher compared to that of UHPLC, but on the other hand it achieved higher peak capacity. Three representative compounds (atrazine, diclofenac and fluazuron) with differing retention and ionisation properties were studied in detail in terms of detection sensitivity. The majority of the ion ratios for the standards in the river water matrix were within ±30% of the average ion ratios. The largest ion suppression occurred for atrazine (−25% matrix effect), a pesticide notorious for poor ionization and poor peak shape issues. The lowest response transitions at 1 μg L−1 for the extracted ions in the river water matrix of atrazine, diclofenac and fluazuron had signal to noise ratios ≥3, with the exception of diclofenac where 5 μg L−1 was the lowest calibration level. The peak area's calibration curve slope and standard deviation in the detector's response determination of limit of quantification (LOQ) were between 5.2 and 30.4 μg L−1.</text>
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                <text>Analytical Methods V.12, NO. 3, 2020. -- P.239-246</text>
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                <text>Despite being the subject of intensive research, tuberculosis, caused by &lt;em&gt;Mycobacterium tuberculosis&lt;/em&gt;, remains at present the leading cause of death from an infectious agent. Secreted and cell wall proteins interact with the host and play important roles in pathogenicity. These proteins are explored as candidate diagnostic markers, potential drug targets or vaccine antigens, and more recently special attention is being given to the role of their post-translational modifications. With the purpose of contributing to the proteomic and glycoproteomic characterization of this important pathogen, we performed a shotgun analysis of culture filtrate proteins of &lt;em&gt;M&lt;/em&gt;. &lt;em&gt;tuberculosis&lt;/em&gt; based on a liquid nano-HPLC tandem mass spectrometry and a label-free spectral counting normalization approach for protein quantification. We identified 1314 &lt;em&gt;M&lt;/em&gt;. &lt;em&gt;tuberculosis&lt;/em&gt; proteins in culture filtrate and found that the most abundant proteins belong to the extracellular region or cell wall compartment, and that the functional categories with higher protein abundance factor were virulence, detoxification and adaptation, and cell wall and cell processes. We could identify a group of proteins consistently detected in previous studies, most of which were highly abundant proteins. In culture filtrate, 140 proteins were predicted to contain one of the three types of bacterial N-terminal signal peptides. Besides, various proteins belonging to the ESX secretion systems, and to the PE and PPE families, secreted by the type VII secretion system using nonclassical secretion signals, were also identified. O-glycosylation was identified in 46 proteins, many of them lipoproteins and cell wall associated proteins. Finally, we provide proteomic evidence for 33 novel O-glycosylated proteins, aiding to the glycoproteomic characterization of relevant antigenic membrane and exported proteins. These findings are expected to collaborate with the research on pathogen derived biomarkers, virulence factors and vaccine candidates, and to provide clues to the understanding of the pathogenesis and survival strategies adopted by &lt;em&gt;M&lt;/em&gt;. &lt;em&gt;tuberculosis&lt;/em&gt;.</text>
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                <text>The biodiesel industry generates crude glycerol (CG) at the rate of 10% of the amount of biodiesel produced. With the production of this biofuel increasing, a large amount of CG is produced each year. This results in low price for it and its final disposal becomes a significant problem. Herein, steam reforming represents a viable route to valorize this biodiesel by-product into hydrogen. The performance of Ni&amp;ndash;La&amp;ndash;Zr catalysts (15 wt%) for steam reforming of a CG without any purification treatment has been investigated at atmospheric pressure and at 500 &amp;deg;C and 650 &amp;deg;C. These materials were prepared using a coprecipitation method with oxalic acid. At 650 &amp;deg;C, the catalyst calcined at 850 &amp;deg;C presents higher catalytic activity and stability and displays better resistance to deactivation by carbon formation than the catalyst calcined at 700 &amp;deg;C. This behavior can be attributed to several causes: higher surface nickel content, higher catalyst reducibility and the presence of oxygen vacancies formed as a consequence of the partial substitution of nickel atoms into the pyrochlore structure during the formation of the biphasic system, which is related to mechanisms that act in the removal of carbon deposits on the catalyst.</text>
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                <text>Biomass and Bioenergy. V. 135, 2020. -- P. 1-7.--3105508</text>
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                <text>The production of H2-rich gaseous mixtures from steam and oxidative steam reforming of crude glycerol was investigated on catalysts with general formula Ni(12&amp;thinsp;wt.%)-La2(Ce1-xZrx)2O7 (x&amp;thinsp;=&amp;thinsp;0, 0.5, 1). The catalysts were prepared by the polymerized complex method based on the Pechini-type reaction route and characterized by a variety of physical and spectroscopic techniques. Samples were calcined in air at 850&amp;thinsp;&amp;deg;C for 4&amp;thinsp;h. All catalysts showed a well-defined NiO phase and a mixed oxide with a general formula A2B2O7 (A: La; B: Ce and/or Zr). NiO was completely reduced to metallic Ni on all catalysts under pure H2 at 650&amp;thinsp;&amp;deg;C. Ni reducibility is favored by the presence of Ce. Redox properties of Ce can also explain a synergetic effect leading to the higher basicity of the catalyst containing Ce and Zr (x&amp;thinsp;=&amp;thinsp;0.5). Catalytic tests were performed at 650&amp;thinsp;&amp;deg;C by feeding the fixed-bed tubular reactor with a crude glycerol:water solution containing 30&amp;thinsp;wt% glycerol. The best results were achieved on the catalyst displaying the highest basicity (Ni-La2(Ce0.5Zr0.5)2O7 both under steam and oxidative reforming conditions. Removal of carbon and alkali metals by simple procedures allows to a significant recovery of the initial catalytic activity without changes in their chemical and phase structure.</text>
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                <text>Catalysis Today .v. 344, 2020. -- p. 190-198</text>
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                <text>The high availability of products with high sugar content, particularly among those targeted as children, has been identified as one of the factors that contribute to the childhood obesity epidemic. For this reason, product reformulation has been recommended as one of the strategies that can be implemented to achieve short-term reductions in children&amp;rsquo;s sugar intake. In this context, the objective of this study was to evaluate the feasibility of using cross-modal (taste-odor-texture) interactions as a strategy for reducing the sugar content of products targeted at children, using milk desserts as case study. A series of 5 vanilla milk desserts were formulated: a control sample with 12% added sugar and 4 sugar-reduced samples (7% added sugar) prepared following a 2 &amp;times; 2 experimental design by varying vanilla (0.4% and 0.6% w/w) and starch (4.3% and 4.7% w/w) concentrations. A total of 112 children (8&amp;ndash;12 years old) tasted the desserts and performed a dynamic sensory characterization task using either temporal check-all-that-apply or temporal dominance of sensations. In addition, they assessed the overall liking of all samples. Results showed that sugar-reduced samples did not significantly differ from the control sample in terms of their average overall liking scores. However, individual differences in children&amp;rsquo;s hedonic reaction were found; three clusters of children with distinctive liking patterns were identified. The increase in vanilla and starch concentration led to an increase in overall liking for over 80% of the children. Sensory dynamic profiles revealed significant but subtle differences among samples. Results from the present work suggest that cross-modal interactions could contribute to minimizing the sensory changes caused by sugar reduction, which could enable to achieve larger reductions if implemented in the context of gradual sugar reduction programs.</text>
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                <text>The objectives of this study were: to assess the efficiency of high hydrostatic pressure or ultra-high pressure homogenization against Mycobacterium smegmatis in milk and to discuss whether M. smegmatis can be considered a suitable surrogate for other Mycobacterium spp. in high pressure inactivation trials using milk. Three strains of this specie (CECT 3017, 3020 and 3032) were independently inoculated into both skimmed (0.2% fat) and whole milk (3.4% fat) at an approximate load of 6.5 Log CFU/ml and submitted to HHP treatments at 300, 400 or 500 MPa for 10 m at 6&amp;deg;C and 20&amp;deg;C. Evolution of the surviving cells of the inoculated strains was evaluated analysing milk immediately after the treatments and after 5 and 8 d of storage at 6&amp;deg;C. HHP treatments at 300 MPa were seldom efficient at inactivating M. smegmatis strains, but lethality increased with pressure applied in all cases. Generation of sub-lethal injured cells was observed only after 400 MPa treatments since inactivation at 500 MPa was shown to be complete. Significant differences were not observed due to either temperature of treatment or fat content of milk, except for strain CECT3032, which was shown to be the most sensitive to HHP treatments. Milk inoculated with strain CECT3017 was submitted to ultra-high pressure homogenization (UHPH) treatments at 200, 300 and 400 MPa. Maximum reductions were obtained after 300 and 400 MPa treatments, although less than 3.50 Log CFU/ml were inactivated. UHPH did not cause significant number of injured cells. The usefulness of this species as a marker for pressure-based processing seems limited since it showed greater sensitivity than some pathogenic species including other Mycobacteria reported in previous studies.</text>
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                <text>Objective: The aim of this study was to develop an emulgel for the treatment of rosacea, applying quality by design (QbD). Methods: An emulgel designed to release the active pharmaceutical ingredients (APIs), metronidazole and niacinamide, via an emollient formulation that favors residence time and attenuates facial redness would be an excellent vehicle to develop to treat rosacea. It was decided to design first a vehicle presenting the attributes established in the quality target product profile, and then, after selecting the best formulation, to load the APIs in it to optimize the final emulgel. A design of experiments was introduced to study the effect of formulation variables on quality attributes (adhesion, phase separation by mechanical stress and viscosity) of the emulgels. Response surface methodology and desirability functions were applied for data analysis. After optimization, the final emulgel was further characterized by assay and in vitro release of APIs, attenuation of facial redness, and compared to commercially available metronidazole products regarding API release. Results: The final emulgel gradually released both APIs, reaching approximately 88% within the first 4&amp;thinsp;h, and their profiles were well described by the Higuchi model. Only a light attenuation effect to conceal facial redness was achieved. Conclusions: A metronidazole and niacinamide emulgel, also providing cosmetic assistance, was developed using QbD. The emulgel releases metronidazole faster than the creams, but more gradually than the commercially available gel, providing a realistic time frame of drug delivery in accordance with the expected time of residence of the adhesive emulgel over the affected facial area.</text>
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                <text>&lt;strong&gt;Torregrosa, Annibal&lt;/strong&gt;</text>
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                <text>Drug Development and Industrial Pharmacy v.46, no.2, 2020.-- p. 296-308</text>
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                <text>&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt; (Por favor lea este aviso antes de abrir los documentos u objetos)&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho de autor sobre toda creaci&amp;oacute;&lt;/strong&gt;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006) ADVERTENCIA - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.</text>
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                <text>Several studies have shown that the inclusion of front of pack nutritional warnings is a potentially effective strategy to encourage consumers to avoid products with high content of nutrients associated with non-communicable diseases. In this context, the present work aimed at exploring consumers&amp;rsquo; reaction towards sodium reduction in the context of the implementation of warnings in three scenarios: package evaluation, tasting and intention to re-purchase the product after tasting, using white bread as case study. A total of 171 consumers participated in the study that encompassed four parts. Firstly, consumers received two bread samples with different salt content (2.00% and 1.38% salt) under blind conditions and were asked to rate their overall liking and to indicate the one they preferred. Then, they were asked to look at four bread packages differing in graphic design and the presence of warnings. They had to rate their expected liking and to indicate the one they would purchase. Finally, consumers were asked to try the bread they had previously chosen, to assess their overall liking and to indicate their willingness to purchase it again. Salt content significantly affected consumer hedonic reaction towards the breads. Two consumer segments with different preference for the salt content in bread were identified: 58% of consumers preferred the bread with 2.00% salt while 42% preferred breads with 1.38% salt. However, when looking at the packages the majority of consumers in both groups selected bread packages that did not feature warnings. In addition, after having tried the bread, most consumers were willing to buy the bread they had previously chosen again, which is promising in terms of reaching sustainable changes towards lower salt levels. Results from the present work suggest that, in the case of bread, nutritional warnings have potential to shift consumers&amp;rsquo; preferences to lower sodium content, even after trying the products.</text>
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                <text>Food Research International. v. 134, 2020. -- p. 1-10.--e109239</text>
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                <text>In the present report, we present a combined experimental and theoretical study on the lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) doping mechanism of regioregular poly(3-hexyl thiophene) (P3HT). First, we focus on the effects of LiTFSI doping in both crystalline and amorphous structures of P3HT by performing a complete structural analysis supported by classical molecular dynamics (MD) calculations. Then, we study the effects of LiTFSI doping on electronic properties such as charge transfer and charge transport by performing Raman and impedance spectroscopy, in both cases supported by density of functionals theory (DFT) calculations using periodic boundary conditions. Our structural analysis suggests that the LiTFSI dopant is mainly located in the amorphous region and only a small fraction is located in the crystalline region. In addition, our DFT calculations also suggest that the LiTFSI dopant can effectively act as an electronic acceptor only when it is located in the vicinity of and is accessible to the thiophene rings of P3HT due to the formation of a &amp;pi;&amp;middot;&amp;middot;&amp;middot;Li chemical bond as an anchoring mechanism, permitting the electronic charge loss of thiophene rings through the sulfonyl groups. A thorough understanding of the LiTFSI doping mechanism of poly(alkyl thiophenes) (P3HT in this particular case) is crucial to elucidating not only the electronic but also the eventual mixed ionic&amp;ndash;electronic transport mechanism and its promising properties, particularly as electrodes for lithium ion battery applications.</text>
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