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                <text>Se presentan dos nuevos m&amp;eacute;todos de c&amp;aacute;lculo de par&amp;aacute;metros farmacocin&amp;eacute;ticos para f&amp;aacute;rmacos que administrados por v&amp;iacute;a extravacular se comportan seg&amp;uacute;n el modelo de un compartimiento con cin&amp;eacute;tica lineal. Ambos son apropiados para el an&amp;aacute;lisis de curvas incompletas (dosis &amp;uacute;nica o dosis m&amp;uacute;ltiple) con valores de concentraci&amp;oacute;n obtenidos hasta 2 Tm&amp;aacute;x aproximadamente&amp;nbsp; y cuando la constante de velocidad de absorci&amp;oacute;n es igual a la constante de velocidad de eliminaci&amp;oacute;n. Se efect&amp;uacute;a un estudio estad&amp;iacute;stico de comparaci&amp;oacute;n con el an&amp;aacute;lisis de regresi&amp;oacute;n por el m&amp;eacute;todo de los residuos |b a partir de datos te&amp;oacute;ricos afectados de error. Tambi&amp;eacute;n se presenta una aplicaci&amp;oacute;n de c&amp;aacute;lculo sobre curvas experimentales de teofilina.</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho de&lt;/strong&gt; autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
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                <text>El cociente de concentraciones tejido/plasma de un f&amp;aacute;rmaco depende de la fracci&amp;oacute;n del gasto card&amp;iacute;aco que irriga cada &amp;oacute;rgano del cuerpo, como asimismo de la fracci&amp;oacute;n libre de sustancia en el plasma sangu&amp;iacute;neo. La concentraci&amp;oacute;n salival de droga cambia en el transcurso de una continua salivaci&amp;oacute;n. Al principio el valor de concentraci&amp;oacute;n representa el nivel de droga libre tisular, pero consecutivamente se transforma en el nivel plasm&amp;aacute;tico libre del f&amp;aacute;rmaco. En 7 pacientes ambulatorios y 22 hospitalizados que recib&amp;iacute;an como tratamiento antiepil&amp;eacute;ptico Fenito&amp;iacute;na o Carbamacepina, se extrajeron muestras salivales estimuladas que se recogieron en dos fracciones en distintas horas del d&amp;iacute;a. El cociente 1ra. fracci&amp;oacute;n / 2da. fracci&amp;oacute;n salival de concentraci&amp;oacute;n de droga (Sl/S2) fue mayor durante la ma&amp;ntilde;ana (07:00-09:00) que en horario vespertino (19:00-21:00), respondiendo al m&amp;aacute;s pronunciado tono simp&amp;aacute;tico matutino. Pacientes cuyas convulsiones no estaban controladas mostraron, algunas horas posteriores a la crisis, cocientes Sl/S2 inferiores que pacientes asintom&amp;aacute;ticos durante el mismo per&amp;iacute;odo del d&amp;iacute;a. Nuestros resultados sugieren que el cociente Sl/S2 de concentraciones salivales de f&amp;aacute;rmaco podr&amp;iacute;a estar ligado con el tono simp&amp;aacute;tico de los individuos, teniendo en cuenta su influencia sobre la regulaci&amp;oacute;n del aparato circulatorio.&lt;br /&gt;&lt;br /&gt;</text>
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                <text>UaY is the specific ZnII2Cys6 transcriptional activator of the purine utilisation pathway in Aspergillus nidulans. Previous work has determined the consensus binding sequence by EMSA and foot-printing. We determine here that it binds as a dimer to its cognate CGG-N6-CCG sites. We identify the uaY109 mutation, which has been shown to affect differentially the expression of a number of UaY-regulated genes, as a F112I substitution in the DNA-binding motif dimerisation domain. We isolated back mutants, revertants carrying different residues at the same position (I112N and I112M) and intragenic suppressors mapping in the first loop of the Zn cluster (N75T and N75K).We have analysed the original mutant and its revertants by growth tests and by their effects on the mRNA steady states of five UaY-regulated genes. We have determined the effect of the different mutations on UaY dimerisation, on the apparent Kdiss of the UaY DNA-binding domain to appropriate DNA sequences and on the methylation interference pattern. We have attempted to rationalise these phenotypes by modelling the UaY DNA binding domain on the structure of the highly similar Ppr1p. However, modelling of the wild-type and mutant proteins provides only a partial explanation for the observed phenotypes. This suggests that the mutated residues may have other roles besides the obvious ones inferred from their position in the sequence and by the similarity of UaY and Ppr1p</text>
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                <text>Fungals Genetics and Biology&amp;nbsp; v. 49, no. 9, 2012. -- p. 731-743</text>
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                <text>A rapid quantitative TLC method for the determination of glycoalkaloids in potato leaves and tubers is described. The method includes a microscale extraction, a simple clean-up step and quantification by TLC scanning. The method is valuable for the determination of glycoalkaloids when many samples have to be evaluated as in potato breeding programmes.</text>
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                <text>The habit of drinking mate is very strongly established in the Rio de la Plata region of South America. A chemical study of the commercial herbal material and of the infusion itself is described.</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Glycosidases represent excellent green chemistry alternatives as catalysts for the synthesis of glycosides, and in particular their stereoselectivity allows the production of anomerically pure glycosides, in only one reaction step using mild reaction conditions. Here, we report the enzymatic synthesis and structural characterization of 3-aminopropyl-1-O-&amp;beta;-D-galactopyranoside. Optimal reaction conditions for the transgalactosylation reaction were 100&amp;thinsp;mM lactose, 500&amp;thinsp;mM 3-amino-1-propanol and 24 h of incubation at 50&amp;thinsp;&amp;deg;C with 6 U/mL of &amp;beta;-galactosidase from Aspergillus oryzae. The fact that the synthesis of 1-propyl-2-O-&amp;beta;-D-galactopyranoside using 1-amino-2-propanol as acceptor was not achieved, and that N-glycoside formation was not observed, confirms the selectivity of &amp;beta;-galactosidase for the synthesis of O-glycosides, and particularly for primary alcohols. The synthesized galactosides were evaluated for their ability to interact with bovine spleen galectin-1 (Gal-1) by using the hemagglutination inhibition assay; results demonstrated that 3-aminopropyl-1-O-&amp;beta;-D-galactopyranoside may be considered as a functionalized galactose moiety more than an efficient Gal-1 inhibitor. The proposed approach constitutes a promising tool for the generation of glycopolymers and glyconanoparticles with potential applications in the development of biosensors as well as construction blocks in chemical synthesis.</text>
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                <text>&lt;strong&gt;Porci&amp;uacute;ncula Gonz&amp;aacute;lez, Cecilia&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Rodr&amp;iacute;guez, Ernesto&lt;/strong&gt;</text>
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                <text>Biocatalysis and Biotransformation v. 33, no. 4, 2015. -- p. 197-207</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt; &lt;br /&gt; (Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt; &lt;br /&gt; (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>�B-Galactosidases from Escherichia coli, Kluyveromyces lactis and Aspergillus oryzae were used to characterize the potential for enzyme stabilization of a two-step strategy: (i) immobilization on glutaraldehyde-agarose (Glut90), (ii) subsequent generation of a hydrophilic nano-environment by reaction with polyaldehyde-dextran polymer (Glut90-Pal), followed by polyamine-dextran polymer (Glut90-Pal-Pam). The derivatives were characterized by kinetics parameters, co-solvent (ethanol and acetone) and temperature stability. Hydrophilization achieved important co-solvent stabilization in all cases. One of the most remarkable results obtained was a 25-fold increase in the half-life of the A. oryzae Glut90-Pal-Pam derivative in 50% (v/v) acetone. Stabilization achieved in very drastic co-solvent concentrations is directly related to the hydrophilization of the nano-environment. The KM values show that the hydrophilic shell appears to behave as an open structure and may create a &amp;ldquo;partition effect&amp;rdquo; that protects the enzymes from denaturation. These results show the potential of hydrophilization for building up additional stabilization of immobilized enzymes which would make possible the development of industrial applications</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>The effect of increasing concentration of co-solvents on the stability of both soluble and immobilized E. coli �-galactosidase was studied. The enzyme was immobilized on to glutaraldehyde-agarose and the co-solvents tested were: N,N-dimethylformamide (DMF), ethanol, acetone, and dioxane (6&amp;ndash;36% v/v). Deactivation kinetics were analyzed according to the two-step deactivation model proposed by Henley and Sadana. A multi-temperature study revealed that the immobilized derivative is considerably more stable than the soluble enzyme, even at high temperature where the half life of the soluble enzyme is less than 1 h. Enzyme immobilization did achieve thermal and solvent stabilization at low concentrations, but this task proved more difficult with increasing co-solvent concentrations. In the case of ethanol and acetone, with the increase in co-solvent concentration, the immobilized derivative became less stable than the soluble enzyme. Immobilization may impose certain constraints on the protein structure which have the effect of sensitizing it to denaturation at high co-solvent concentrations. Complementary stabilization strategies are, therefore, being studied.</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;&#13;
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                <text>&lt;strong&gt;Redox effect on volatile compound formation in wine during fermentation by Saccharomyces cerevisiae&lt;/strong&gt;</text>
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                <text>&lt;p&gt;Although redox state is a well-known key process parameter in microbial activity, its impact on wine volatile aroma compounds produced during fermentation has not been studied in detail. In this study we report the effect of reductive and microaerobic conditions on wine aroma compound production using different initial amounts of yeast assimilable nitrogen (YAN: 180 and 400 mg N/l) in a simil grape must defined medium and two S. cerevisiae strains commonly used in wine-making. In batch fermentation culture conditions, reductive conditions were obtained using flasks plugged with Muller valves filled with sulphuric acid; while microaerobic conditions were attained with defined cotton plugs. It was found that significant differences in redox potential were obtained using the different plugs, and with variation of over 100 mV during the main fermentation period. Significant differences in the final concentration of higher alcohols, esters and fatty acids were attributed to differences in the redox state in the medium in both strains. A consistent increase in esters and medium chain fatty acids, as well as a decrease of higher alcohols and isoacids, was seen under reductive fermentation conditions. Interestingly, 1-propanol, d-butyrolactone and ethyl lactate concentrations, showed no significant variation under the different redox conditions. A better understanding of the influence of redox state of the fermentation medium on the composition of volatile compounds in wine could enable improvement of vinification management. From a microbiological standpoint results presented here will contribute to the standardization of data models for the application of metabolic footprinting methods for wine yeast strain phenotyping and characterization&lt;/p&gt;</text>
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                <text>Food Chemistry v. 134, no. 2, 2012. -- p. 933-939</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;&#13;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;</text>
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                <text>Under humid climatic conditions, crop loss due to bunch rot can be as high as 100% in a given year and,in many cases, it becomes the main factor determining harvest. Although the effectiveness of fruit zoneleaf removal depends on initial fruit microclimate, it is generally extremely effective at reducing bunchrots. The impact of leaf removal on Sauvignon blanc must composition and wine sensory attributes, isin general considered detrimental. High temperature achieved as a result of excessive bunch exposuremay negatively affect primary and secondary grape metabolites responsible for its typical wine style.The main objective of this work was to determine, if kaolin application over clusters, reduce sunburnand other berry damage associated with excessive fruit exposure. Three treatments were evaluated:untreated control (C); fruit zone leaf removal (LR); and fruit zone leaf removal plus kaolin application(LR + K). Kaolin application significantly reduced berry temperature, sunburn and other berry damageassociated with excessive fruit exposure. Wines from the LR + K treatment had the highest sensory scoresof all three treatments and were related more to a high intensity of fruity and tropical notes rather thanto the absence of green notes as a consequence of leaf remova</text>
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                <text>&lt;strong&gt;Ferrari, Virginia&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Gepp, Vivienne&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Disegna, Edgardo&lt;/strong&gt;</text>
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                <text>European Journal of Agronomy v. 50, 2013. -- p. 75-81</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Volatile composition and aroma profile of Uruguayan Tannat wines&lt;/strong&gt;</text>
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                <text>Tannat is a red variety of Vitis vinifera that has becomethe major variety for the production of premiumred wines in Uruguay. Due to its small cultivation around theworld, research on the viticulture and enology of this variety is still necessary to improvewine quality. In this context, the aimof the presentworkwas to characterize the aroma profile of Uruguayan Tannat wines using chemical and sensory methodologies. The volatile composition of ten Uruguayan Tannat wines, sold in the international market, was studied by gas-chromatography (GC&amp;ndash;MS). Sixty two volatile compounds were identified using GC&amp;ndash;MS, being alcohols and esters the most abundant compounds. Only few volatile compounds were found at concentrations higher than their odor threshold in all samples. Sensory characterization of wine aroma was characterized by a panel of wine professionals using projective mapping. Red fruits, fruity, dry fruits, and woody were the main descriptors used for describing similarities and differences in the aroma profile of the wines. Projective mapping sorted samples into four main groups. Partial least square regression (PLSR) enabled to explain many of the most important sensory descriptors (woody, earthy, phenolic, sulfur, chemical and microbiological) through volatile composition.</text>
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                <text>Food Research International v. 69, 2015. -- p. 244-255</text>
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                <text>&lt;p align="LEFT"&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;/p&gt;&#13;
&lt;p&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p style="margin-bottom: 0cm;"&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>http://dx.doi.org/10.1016/j.foodres.2014.12.029</text>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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                <text>&lt;strong&gt;Bioactive-guided identification of labdane diterpenoids from aerial parts of Aristeguietia glutinosa as anti-Trypanosoma cruzi agents&lt;/strong&gt;</text>
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                <text>ARISTEGUIETIA GLUTINOSA</text>
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                <text>2012</text>
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                <text>A bioactive-guided investigation of the hydro-ethanolic extract of aerial parts of Aristeguietia glutinosa Lam. resulted in the isolation of two diterpenoids, (+)-15-hydroxy-labd-7-en-17-al (1) and (+)-13,14,15,16-tetranor-labd-7-en-17,12-olide (2), as the anti-Trypanosoma cruzi active principles. The structures of 1 and 2 were determined by spectroscopic analysis. The hydro-ethanolic extract showed anti-T. cruzi activity (IC50 = 19.6 microg/mL) whereas the isolated compounds 1 and 2 were near to seven- and one and a half-fold (IC50 = 3.0 and 15.6 microg/mL), respectively more active than the original extract. Labdene 1, equipotent as the reference compound (Nifurtimox), displayed low hemolytic activity, low toxicity against murine macrophages, and absence of mutagenicity. These results support the vernacular medicinal use of this plant as an anti-T. cruzi agent.&lt;br /&gt;&lt;br /&gt;</text>
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                <text>&lt;strong&gt;Varela, J.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Lavaggi Destro, Mar&amp;iacute;a Laura&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Cabrera, M.&lt;/strong&gt;</text>
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                <text>&lt;a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=e763cfcf3da319d62989630831835a77" target="_blank"&gt;&lt;strong&gt;Rodr&amp;iacute;guez, A.&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>Natural Product Communications v. 7, no. 9, 2012. -- p. 1139-1142</text>
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                <text>&lt;p style="margin-bottom: 0cm;"&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt;&lt;br /&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt;&lt;br /&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt;&lt;br /&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt;&lt;br /&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Acidic wet deposition as a global problem in Uruguay&lt;/strong&gt;</text>
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                <text>Presentation of the first data obtained from April to November, 1991 with the available resources. Determination of the pH, nitrates, sulfates, lead, cadmium, copper and zinc in samples of rainwater collected in the Cerro Largo department and data from rainwater collected in some other similar agricultural regions of Uruguay to make comparisons. Meteorological and atmospheric SO2 levels data are given as background information</text>
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                <text>American Chemical Society, Division of Petroleum Chemistry&amp;nbsp; v. 87, no. 4, 1992. -- p. 1506-12</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>ISSN: 0569-3799</text>
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                <text>&lt;strong&gt;Groundwater Arsenic Content in Raig&amp;oacute;n Aquifer System (San Jos&amp;eacute;, Uruguay)&lt;/strong&gt;</text>
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                <text>As a Medical Geology research issue, an environmental arsenic risk assessment study in the most important sedimentary aquifer in southern Uruguay is presented. The Raig&amp;oacute;n Aquifer System is the most exploited in Uruguay. It has a surface extent of about 1,800 square kilometres and 10,000 inhabitants in San Jose Department, where it was studied. Agriculture and cattle breeding are the main economic activities and this aquifer is the basic support. The groundwater sampling was done on 37 water samples of PRENADER (Natural Resources Management and Irrigation Development Program) wells. Outcropping sediments of Raig&amp;oacute;n Formation and the overlying Libertad Formation were also sampled in the Kiy&amp;uacute; region. The analyses were performed by inductively coupled plasma-optical emission spectrometry (ICP-OES) and inductively coupled plasma-mass spectrometry (ICP-MS). The results showed 80% samples with arsenic levels exceeding the 10 &amp;mu;g/l of WHO as limit for waters, and 11% exceeds the 20 &amp;mu;g/l limit of uruguayan regulation. The median, maximum and minimum water arsenic concentrations determined have been 14.24, 24.19 and 1.44 &amp;mu;g/l, respectively. On the other hand, nine sediment samples of Raig&amp;oacute;n and Libertad Formations in Kiy&amp;uacute; region were analysed and yielded median, maximum and minimum arsenic concentrations of 5.03, 9.82 and 1.18 ppm, respectively. This issue leads to the supposition that the population, as well as industrial and agricultural activities, are consuming water with arsenic concentrations over the national and international maximum recommended limit.&lt;br /&gt;Como una aproximaci&amp;oacute;n a la Geolog&amp;iacute;a M&amp;eacute;dica en Uruguay se presentan los resultados de un estudio sobre el estado del Sistema Acu&amp;iacute;fero Raig&amp;oacute;n en relaci&amp;oacute;n a la presencia de ars&amp;eacute;nico, en el entendido que es el acu&amp;iacute;fero m&amp;aacute;s explotado en el pa&amp;iacute;s. En particular, el &amp;aacute;rea cubierta por este trabajo es de unos 1800 km2 en un territorio con una poblaci&amp;oacute;n de unos 10,000 habitantes en el departamento de San Jos&amp;eacute;. La colecta de aguas del acu&amp;iacute;fero se realiz&amp;oacute; en perforaciones de la base de datos de PRENADER (Proyecto de Manejo de Recursos Naturales y Desarrollo del Riego) obteni&amp;eacute;ndose 37 muestras, y sobre 9 muestras de sedimentos tanto de la Formaci&amp;oacute;n Raig&amp;oacute;n como la suprayacente Libertad en afloramientos de la regi&amp;oacute;n de Kiy&amp;uacute;. Los an&amp;aacute;lisis fueron realizados mediante ICP-MS. Un 80% de las muestras de aguas analizadas mostraron contenidos mayores a los l&amp;iacute;mites de la OMS (10 &amp;mu;g/l) y un 11% de las muestras superaron el l&amp;iacute;mite de la OSE (20 &amp;mu;g/l). El valor medio, m&amp;aacute;ximo y m&amp;iacute;nimo determinado en el caso del agua subterr&amp;aacute;nea fue de 14.24, 24.19 and 1.44 &amp;mu;g/l, respectivamente. Por otro lado, las nueve muestras de sedimentos analizados mostraron concentraciones media, m&amp;aacute;xima y m&amp;iacute;nima de ars&amp;eacute;nico de 5.03, 9.82 and 1.18 ppm, respectivamente, descart&amp;aacute;ndose en principio un origen geog&amp;eacute;nico.</text>
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                <text>&lt;a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=eb363e18b4425600161a60511e5af5cf" target="_blank"&gt;&lt;strong&gt;Ma&amp;ntilde;ay, Nelly&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>&lt;strong&gt;Goso, N.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Fern&amp;aacute;ndez Turiel, J.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Garc&amp;iacute;a Vall&amp;eacute;s, M.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Rejas, M.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Guerequiz, R.&lt;/strong&gt;</text>
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                <text>Revista de la Sociedad Uruguaya de Geología v. 18, 2013. -- p. 20-38&#13;
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                <text>Dr. Claudio Gaucher (Coordinador)</text>
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                <text>Dr. Pedro Oyhant&amp;ccedil;abal</text>
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                <text>&lt;p style="margin-bottom: 0cm;"&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt;&lt;br /&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt;&lt;br /&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt;&lt;br /&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt;&lt;br /&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>The development of a solid-phase biocatalyst based on the reversible covalent immobilization of laccase onto thiol-reactive supports (thiolsulfinate-agarose [TSI-agarose]) was performed. To achieve this goal, laccase-producing strains isolated from Eucalyptus globulus were screened and white rot fungus Trametes villosa was selected as the best strain for enzyme production. Reduction of disulfide bonds and introduction of &amp;ldquo;de novo&amp;rdquo; thiol groups in partially purified laccase were assessed to perform its reversible covalent immobilization onto thiol-reactive supports (TSI-agarose). Only the thiolation process dramatically improved the immobilization yield, from 0% for the native and reduced enzyme to 60% for the thiolated enzyme. Mild conditions for the immobilization process (pH 7.5 and 4&amp;deg;C) allowed the achievement of nearly 100% of coupling efficiency when low loads were applied. The kinetic parameters, pH, and thermal stabilities for the immobilized biocatalyst were similar to those for the native enzyme. After the first use and three consecutives reuses, the insoluble derivative kept more than 80% of its initial capacity for decolorizing Remazol Brilliant Blue R, showing its suitability for color removal from textile industrial effluents. The possibility of reusing the support was demonstrated by the reversibility of enzyme-support binding.</text>
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                <text>A thiol-&amp;beta;-cyclodextrin was synthesized by a simple and environmentally friendly three-step method comprising epoxy activation of &amp;beta;-cyclodextrin, thiosulfate-mediated oxirane opening, and further reduction of the S-alkyl thiosulfate to a thiol group. The final step was optimized by using thiopropyl-agarose, a solid phase reducing agent with many advantages over soluble ones. &amp;beta;-Cyclodextrin thiolation was confirmed by titration with a thiol-reactive reagent, NMR studies, and MALDI-TOF/TOF. Thiolated cyclodextrin had an average value of one thiol group per molecule. Thiol-&amp;beta;-cyclodextrin proved to be an excellent agent for controlling polyphenol oxidase activity. This copper-containing enzyme is responsible for browning in fruits and vegetables. Under the same conditions, thiol-&amp;beta;-cyclodextrin generated a reductive microenvironment that increased the antibrowning effect on Red Delicious apples compared to unmodified &amp;beta;-cyclodextrin.</text>
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                <text>Journal of Agricultural and Food Chemistry v. 61, no. 47, 2013. -- p. 11603-11609</text>
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                <text>&lt;p style="margin-bottom: 0cm;"&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;br /&gt;&lt;br /&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;br /&gt;&lt;br /&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes&lt;br /&gt;&lt;br /&gt;(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;br /&gt;&lt;br /&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Bacterial adenosine 5&amp;rsquo;-diphosphate-ribosylating toxins are encoded by several human pathogens, such as Pseudomonas aeruginosa (exotoxin A (ETA)), Corynebacterium diphtheriae (diphtheria toxin (DT)), and Vibrio cholerae (cholix toxin (CT)). The toxins modify eukaryotic elongation factor 2, an essential human enzyme in protein synthesis, thereby causing cell death. Targeting external virulence factors, such as the above toxins, is a promising alternative for developing new antibiotics, while at the same time avoiding drug resistance. This study aims to establish a reliable computational methodology to find a &amp;ldquo;silver bullet&amp;rdquo; able to target all three toxins. Herein, we have undertaken a detailed analysis of the active sites of ETA, DT, and CT, followed by the determination of the most appropriate selection of the size of the docking sphere. Thereafter, we tested two different approaches for normalizing the docking scores and used these to verify the best target (toxin) for each ligand. The results indicate that the methodology is suitable for identifying selective as well as multitoxin inhibitors, further validating the robustness of inverse docking for target-fishing experiments.</text>
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            <name>Title</name>
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                <text>&lt;strong&gt;Structural insights into human microsomal epoxide hydrolase by combined homology modeling, molecular dynamics simulations and molecular docking calculations&lt;/strong&gt;</text>
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                <text>A new homology model of human microsomal epoxide hydrolase was derived based on multiple templates. The model obtained was fully evaluated, including MD simulations and ensemble-based docking, showing that the quality of the structure is better than that of only previously known model. Particularly, a catalytic triad was clearly identified, in agreement with the experimental information available. Analysis of intermediates in the enzymatic mechanism led to the identification of key residues for substrate binding, stereoselectivity, and intermediate stabilization during the reaction. In particular, we have confirmed the role of the oxyanion hole and the conserved motif (HGXP) in epoxide hydrolases, in excellent agreement with known experimental and computational data on similar systems. The model obtained is the first one that fully agrees with all the experimental observations on the system.</text>
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                <text>&lt;a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=f4e5f5966e2f949cc6ad308da8978be6" target="_blank"&gt;&lt;strong&gt;Saenz M&amp;eacute;ndez, Patricia&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>&lt;strong&gt;Katz, Aline&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;P&amp;eacute;rez Kempner, Mar&amp;iacute;a Luc&amp;iacute;a&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;V&amp;aacute;zquez, Marta&lt;/strong&gt;</text>
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                <text>Proteins: Structure, Function and Bioinformatics v. 85, no. 4, 2017. -- p. 720-730</text>
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            <name>Publisher</name>
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                <text>Wiley</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>DOI: 10.1002/prot.25251</text>
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        <name>Toxicidad</name>
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