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                <text>&lt;strong&gt;Purificaci&amp;oacute;n, caracterizaci&amp;oacute;n funcional y estructural de ciste&amp;iacute;n proteinasas presentes en frutos maduros de Bromelia ant&amp;iacute;acantha. Evaluaci&amp;oacute;n de sus posibles aplicaciones&lt;/strong&gt;</text>
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                <text>Las proteasas o peptidasas, son enzimas que catalizan la hidr&amp;oacute;lisis del enlace pept&amp;iacute;dico de las prote&amp;iacute;nas. Se describen cinco clases de acuerdo a su mecanismo catal&amp;iacute;tico: proteasas asp&amp;aacute;rticas, de ciste&amp;iacute;na, met&amp;aacute;licas, de serina y de treonina. Estas enzimas desempe&amp;ntilde;an papeles clave en todos los organismos vivos. A pesar de catalizar la misma reacci&amp;oacute;n, la especificidad por el sustrato suele ser marcadamente diferente para cada proteasa, haci&amp;eacute;ndolas mol&amp;eacute;culas &amp;uacute;nicas. El estudio de nuevas peptidasas, aporta informaci&amp;oacute;n valiosa permitiendo una mayor comprensi&amp;oacute;n de las propiedades que definen la especificidad y la relaci&amp;oacute;n estructura-funci&amp;oacute;n, adem&amp;aacute;s de generar nuevas herramientas biotecnol&amp;oacute;gicas. En este contexto, se trabaj&amp;oacute; con frutos maduros de Bromelia antiacantha Bertol., una especie aut&amp;oacute;ctona, perteneciente a la familia Bromeliaceae. Partiendo del extracto de estos frutos y conociendo los puntos isoel&amp;eacute;ctricos de sus componentes proteicos, se emple&amp;oacute; una metodolog&amp;iacute;a de purificaci&amp;oacute;n simple que permiti&amp;oacute; separar tres nuevas peptidasas que denominamos: Antiacantha&amp;iacute;na A (AntA), Antiacantha&amp;iacute;na B (AntB) y Antiacantha&amp;iacute;na C (AntC). La caracterizaci&amp;oacute;n bioqu&amp;iacute;mica de las tres enzimas mostr&amp;oacute; que ellas tienen propiedades similares y t&amp;iacute;picas de las ciste&amp;iacute;n peptidasas</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Los estudios de exploraci&amp;oacute;n y prospecci&amp;oacute;n realizados en Uruguay hasta el momento no han podido probar la existencia de yacimientos de combustibles f&amp;oacute;siles tradicionales (petr&amp;oacute;leo, carb&amp;oacute;n mineral y gas natural) econ&amp;oacute;micamente viables para su explotaci&amp;oacute;n. En el &amp;uacute;ltimo siglo, Uruguay ha tenido que importar petr&amp;oacute;leo y gas natural para satisfacer gran parte de su demanda energ&amp;eacute;tica. Esto ha tenido un alto costo econ&amp;oacute;mico determinando una fuerte dependencia del exterior, pero tambi&amp;eacute;n en los &amp;uacute;ltimos tiempos ha sido impulsado el desarrollo y el uso de fuentes renovables de energ&amp;iacute;a as&amp;iacute; como la exploraci&amp;oacute;n y estudio de aplicaciones de combustibles f&amp;oacute;siles no convencionales tales como las Lutitas Pirobituminosas. Se han reportado numerosos estudios de co-combusti&amp;oacute;n de Lutitas Pirobituminosas con otros combustibles como los lodos de tratamiento de efluentes y lignina, demostrando que esta tecnolog&amp;iacute;a puede ser una alternativa viable para la producci&amp;oacute;n de energ&amp;iacute;a. Sin embargo, existen pocos trabajos de investigaci&amp;oacute;n relacionados con la combusti&amp;oacute;n de Lutitas Pirobituminosas con biomasa. Dada la abundancia de residuos de biomasa generados por la actividad agroforestal en Uruguay, este trabajo de tesis propone realizar un estudio riguroso de los fundamentos de la co-combusti&amp;oacute;n de las Lutitas Pirobituminosas uruguayas con residuos de biomasa (C&amp;aacute;scara de Arroz, Eucalyptus grandis y Orujo de Uva), teniendo en cuenta par&amp;aacute;metros ambientales. Se emplearon t&amp;eacute;cnicas para caracterizar las Lutitas Pirobituminosas uruguayas, los residuos de biomasa y las mezclas preparadas a partir de estos, desde el punto de vista estructural, morfol&amp;oacute;gico y fisicoqu&amp;iacute;mico. Adem&amp;aacute;s, se evalu&amp;oacute; la cin&amp;eacute;tica y par&amp;aacute;metros t&amp;eacute;rmicos de diferentes mezclas de los combustibles. Durante el desarrollo de la tesis de posgrado se realiz&amp;oacute; una pasant&amp;iacute;a en la Universidad de Queensland con el fin de realizar otros an&amp;aacute;lisis que permitieron obtener informaci&amp;oacute;n importante de los combustibles y de la cin&amp;eacute;tica de combusti&amp;oacute;n mediante el empleo de an&amp;aacute;lisis termogravim&amp;eacute;trico acoplado a FTIR, Transient plane Source y Calorimetr&amp;iacute;a de Cono. Los resultados indicaron que la relaci&amp;oacute;n entre el calor efectivo de combusti&amp;oacute;n y el poder calor&amp;iacute;fico superior y las emisiones gaseosas de contaminantes ix atmosf&amp;eacute;ricos de las Lutitas Pirobituminosas se ven mejoradas con la adicci&amp;oacute;n de biomasa para la granulometr&amp;iacute;a empleada para la realizaci&amp;oacute;n de los an&amp;aacute;lisis, tanto desde el punto de vista cin&amp;eacute;tico como t&amp;eacute;rmico. Se ha llegado a establecer un rango de composici&amp;oacute;n de mezcla a ser utilizado principalmente con residuos de Eucaliptus grandis, la cual debe ser menor a 50 % de Lutitas Pirobitumino</text>
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                <text>&lt;strong&gt;Castiglioni, Jorge&lt;/strong&gt; (director de Tesis)</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Las galectinas, son prote&amp;iacute;nas que poseen alta afinidad por &amp;beta;-galact&amp;oacute;sidos. Dichas prote&amp;iacute;nas poseen un dominio conservado denominado Dominio de Reconocimiento a Carbohidrato (DRC), donde se da la interacci&amp;oacute;n con sus ligandos. En particular las galectinas 1 y 3 han sido ampliamente estudiadas, ya que se encuentran vinculadas a distintos procesos biol&amp;oacute;gicos, entre otros adhesi&amp;oacute;n celular, extravasaci&amp;oacute;n celular y met&amp;aacute;stasis, favoreciendo el desarrollo tumoral. Por esta raz&amp;oacute;n se han convertido en un blanco prometedor para la terapia contra el c&amp;aacute;ncer ya que se podr&amp;iacute;a inhibir su capacidad pro-tumorog&amp;eacute;nica bloqueando el sitio de uni&amp;oacute;n a carbohidratos con glic&amp;oacute;sidos que compitan con sus ligandos naturales. En este marco el objetivo general de esta tesis fue establecer una estrategia que permitiera el dise&amp;ntilde;o racional de inhibidores de galectinas con potencial actividad antitumoral. Para ello se propuso el desarrollo in silico de modelos computacionales, capaces de predecir la naturaleza de la interacci&amp;oacute;n entre los ligandos seleccionados y las galectinas. Por otro lado se realiz&amp;oacute; la s&amp;iacute;ntesis enzim&amp;aacute;tica de dichos compuestos y la evaluaci&amp;oacute;n de su actividad biol&amp;oacute;gica mediante estudios in vitro. Se sintetizaron enzim&amp;aacute;ticamente cinco galact&amp;oacute;sidos: &amp;beta;-D-Galp-(16)-&amp;beta;-D-Galp-(14)-D-Glcp (2), la mezcla &amp;beta;-D-Galp-(16)-&amp;beta;-D-Glcp-(14)-D-Glcp (3) y &amp;beta;-D-Galp-(13)-&amp;beta;-D-Glcp-(14)-D-Glcp (4), &amp;beta;-D-Galp-(16)-&amp;beta;-D-GlcNAcp-(14)-D-GlcNAcp (6) y el galact&amp;oacute;sido de car&amp;aacute;cter arom&amp;aacute;tico bencil-&amp;beta;-D-galactopiran&amp;oacute;sido (5), aplicando el sistema de transglicosilaci&amp;oacute;n catalizado por la enzima &amp;beta;-galactosidasa de Aspergillus oryzae. Posteriormente se estudi&amp;oacute; la afinidad y especificidad de uni&amp;oacute;n in silico de las galectinas 1 y 3 y los &amp;beta;-galacto-compuestos sintetizados junto con otros disac&amp;aacute;ridos de origen comercial. Para ello se aplicaron distintas simulaciones de Din&amp;aacute;mica Molecular, en combinaci&amp;oacute;n con experimentos de Docking Molecular, en la b&amp;uacute;squeda de generar modelos computacionales capaces de reproducir y predecir las interacciones entre las galectinas y sus ligandos. A su vez, se estimaron las energ&amp;iacute;as libres de uni&amp;oacute;n (&amp;Delta;G) de cada complejo galectina-ligando obtenido. Con el objetivo de validar los modelos te&amp;oacute;ricos obtenidos, se estudi&amp;oacute; la interacci&amp;oacute;n galectina-ligando mediante dos ensayos biol&amp;oacute;gicos in vitro: ensayo en fase s&amp;oacute;lida e ITC. Los resultados obtenidos, mostraron una &amp;oacute;ptima correlaci&amp;oacute;n entre los resultados experimentales y te&amp;oacute;ricos, validando los modelos generados previamente. A pesar de la complejidad intr&amp;iacute;nseca del modelado de carbohidratos y lo expuesto al disolvente del DRC, en esta tesis se obtuvieron modelos te&amp;oacute;ricos preliminares, capaces de sortear dichas dificultades. Si bien los ligandos sintetizados no mostraron afinidades superiores a las de los ligandos de referencia (lactosa y N-acetil-lactosamina), los resultados obtenidos, constituyen un estudio detallado de la naturaleza de dichas interacciones. De hecho se pudieron establecer diversos aspectos de la naturaleza qu&amp;iacute;mica del ligando que influyen en la interacci&amp;oacute;n con la galectina. Por un lado la naturaleza del enlace entre la galactosa y el segundo az&amp;uacute;car es fundamental, siendo los enlaces &amp;beta;-(14) y &amp;beta;-(13) los que presentan mayor afinidad. Otro aspecto a tener en cuenta es la identidad de dicho az&amp;uacute;car, determin&amp;aacute;ndose que la presencia del grupo N-acetilo en el carbono 2 del residuo glucopiran&amp;oacute;sico, mejora la interacci&amp;oacute;n con el subsitio D de las galectinas, tanto 1 como 3. Finalmente, la adici&amp;oacute;n de un tercer az&amp;uacute;car no resulta beneficioso respecto a la energ&amp;iacute;a de uni&amp;oacute;n. Esto no quiere decir que ligandos m&amp;aacute;s grandes no puedan ser m&amp;aacute;s afines por la galectina, sino que en principio estructuras de tipo trisac&amp;aacute;ridos no ser&amp;iacute;a suficiente y no evidencian diferencias respecto a los disac&amp;aacute;ridos. Finalmente los modelos te&amp;oacute;ricos generados en esta tesis representan un importante insumo para el dise&amp;ntilde;o y s&amp;iacute;ntesis de inhibidores de galectinas m&amp;aacute;s potentes.</text>
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&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Information rich chromatographic separations of natural samples : the analysis of antioxidants in coffee using post column derivatisation and the CUPRAC assay on narrow bore reaction flow HPLC columns&lt;/strong&gt;</text>
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                <text>The cupric reducing antioxidant capacity (CUPRAC) assay is an established procedure used to measure antioxidant content. Recently, the CUPRAC assay was adapted for use in post column derivatisation coupled with high performance liquid chromatography (HPLC-PCD). In prior works reported in the literature, CUPRAC HPLC-PCD assays used large post column mixing coils leading to a deterioration in the observed separation efficiency. In this work we used the CUPRAC PCD assay with reaction flow (RF) chromatography. No reaction loop was required, thus preserving the efficiency of the separation. We subsequently compared the performance of the CUPRAC assay to that of an antioxidant assay that we have previously converted to RF chromatography, namely the ferric reducing antioxidant potential (FRAP) assay, both in RF mode. The relative response factor with respect to trolox was measured for both RF assays via the separation of a standard mixture of 16 compounds. Sample analysis of coffee and green tea via both RF assays was used to compare their ability to screen for bioactive candidates in complex natural products. In general, the CUPRAC assay is recommended as the RF PCD methodology of choice as it provided a higher level of performance compared to the FRAP assay, demonstrating less baseline noise interference, greater sensitivity, wider linear dynamic range and better assay precision.</text>
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                <text>&lt;strong&gt;Suktham, Thirada&lt;/strong&gt;</text>
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                <text>Microchemical Journal v. 153, 2020. -- P. 1-6</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Absolute structure of (3aS,5S,7aS,7bS,9aR,10R,12aR,12bS )-7b-hydroxy- 4,4,7a,9a,12a-pentamethyl-10-[(2 R )-6-methylheptan-2-yl]- 2,8,9-trioxooctadecahydrobenzo[d ]indeno[4,5-b ]azepin-5-yl acetate from 62-year-old crystals&lt;/strong&gt;</text>
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                <text>The structure of the title compound, C32H51NO6, was determined from 62-year-old crystals at room temperature and refined with 100 K data in a monoclinic (C2) space group. This compound with a triterpenoid structure, now confirmed by this study, played an important role in the determination of the structure of lanosterol. The mol&amp;shy;ecules pack in linear O&amp;mdash;H⋯O hydrogen-bonded chains along the short axis (b), while parallel chains display weak van der Waals inter&amp;shy;actions that explain the needle-shaped crystal morphology. The structure exhibits disorder of the flexible methyl&amp;shy;heptane chain at one end of the main mol&amp;shy;ecule with a small void around it. Crystals of the compounds were resistant to data collection for decades with the available cameras and Mo K&amp;alpha; radiation single-crystal diffractometer in our laboratory until a new instrument with Cu K&amp;alpha; radiation operating at 100 K allowed the structure to be solved and refined.</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona&lt;/p&gt;</text>
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                <text>Contamination of honey with toxic pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) was assessed by dilution with acidic methanol and analysis through liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) in three different modes. The hybrid linear trap/triple quadrupole (LC-QTRAP) instrument was used in precursor ion scan (PIS), enhanced product ion scan (EPI), and multiple reaction monitoring (MRM) mode. The parent ions from ions at m/z 120 or 138 amus, characteristic of all the toxic PAs and PANOs in the sample were first scanned by PIS. Then, the presence of each contaminant at specific retention times through its MS2 spectrum was confirmed by EPI. Finally, they were quantified in the MRM mode. The method was validated: recoveries 86–111%, relative standard deviation (RSD) &lt;20%, at 20 and 40 μg/kg, except retrorsine, which showed a RSD of 30% at 20 μg/kg. Honey samples were analyzed and five of them showed levels of 40 μg/kg for the sum (PAs + PANOs). This approach allows the simultaneous determination of PAs and PANOs in honey, even if their chemical standards are not available.</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Exploring oxidovanadium (IV) homoleptic complexes with 8-hydroxyquinoline derivatives as prospective antitrypanosomal agents&lt;/strong&gt;</text>
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                <text>VANADIO</text>
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                <text>TRIPANOSOMA CRUZI</text>
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                <text>ANTITRIPANOSOMAS</text>
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                <text>Ten structurally related homoleptic complexes, including three new compounds, were synthesized: five [VIVO(L-H)2] and five [VVO(OCH3)(L-H)2] compounds, where L&amp;ndash;H = L1-H&amp;ndash;L5-H are monodeprotonated L1&amp;ndash;L5 8-hydroxyquinoline derivatives. The compounds were characterized in the solid state and in solution. All complexes show inhibitory activity against Trypanosoma cruzi and Leishmania infantum, most within the 0.2&amp;ndash;5 &amp;mu;M range, T. cruzi being more sensitive to the compounds than L. infantum. The compounds show high selectivities towards the parasite, evaluated using VERO cells as a mammalian cell model, as well as higher activity against T. cruzi than the reference antitrypanosomal drug nifurtimox. The antitrypanosomal activity depends on the nature of the substituents on the 8-hydroxyquinoline moiety, [VIVO(L1-H)2] (L1 = 5,7-dichloroquinolin-8-ol), with IC50 = 0.21 &amp;mu;M, being the most active and selective compound of the series against T. cruzi, and one of the most active against L. infantum (IC50 = 2.5 &amp;mu;M). A trypanocide effect of [VIVO(L1-H)2] was observed when incubating parasites with 10&amp;times; the IC50 value and a late apoptotic/necrotic cell death mechanism is suggested. A preferred association with the soluble protein fraction of cells is also observed (49.4% of total vanadium association). [VIVO(L1-H)2] is shown to affect not only the infection potentiality of cell-derived trypomastigotes but also the proliferation of intracellular amastigotes, the two parasite stages more relevant in the mammalian host after infection. By measuring circular dichroism spectra of solutions containing either human serum apo-transferrin (apo-HTF) or bovine serum albumin (BSA) upon adding the compounds, it is shown that both the free ligands and the VIV- and VV-complexes bind to these proteins. From data determined from fluorescence quenching measurements with BSA, the determined Stern&amp;ndash;Volmer constants are typically slightly higher for the ligand precursors than for the corresponding [VIVO(L1-H)2] compounds, the calculated conditional binding constants at pH = 7.4 being ca. 105&amp;ndash;106. The fluorescence competition measurements with DNA bound to ethidium bromide typically yield relatively small decreases in fluorescence, VIV- and VV-complexes with L1 showing higher quenching.</text>
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                <text>&lt;strong&gt;Scalese, Gonzalo&lt;/strong&gt;</text>
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                <text>&lt;a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=5c1ca4d55a5933a1752b3fc23d7cad09c4e5df59004aa474d3d272c7b6b5d79efe1957de15b2f0c75050b8d88b94dc89f36f3a8703836670d171bb4291eb6134&amp;amp;formato=pdf&amp;amp;convocatoria=21" target="_self"&gt;&lt;strong&gt;Machado, Ignacio&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>&lt;strong&gt;Correia, Isabel&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Bilbao, Luc&amp;iacute;a.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;P&amp;eacute;rez-D&amp;iacute;az, Leticia.&lt;/strong&gt;</text>
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                <text>New Journal of Chemistry&amp;nbsp; v. 43, no. 45, 2019.--p. 17756-17773</text>
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                <text>Royal Society of Chemistry</text>
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            <name>Rights</name>
            <description>Information about rights held in and over the resource</description>
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              <elementText elementTextId="546349">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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        <name>Antitripanosomas</name>
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        <name>Trypanosoma cruzi</name>
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                  <text>Bibliografía Nacional Química</text>
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              <name>Description</name>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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              <elementTextContainer>
                <elementText elementTextId="709199">
                  <text>Departamento de Documentación y Biblioteca</text>
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                  <text>RIQUIM</text>
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          <name>Original Format</name>
          <description>If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data</description>
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              <text>PDF</text>
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                <text>&lt;strong&gt;Anatomical and Biochemical Changes Induced by Gluconacetobacter diazotrophicus Stand Up for Arabidopsis thaliana Seedlings From Ralstonia solanacearum Infection&lt;/strong&gt;</text>
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                <text>ANATOMIA</text>
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                <text>GLUCONACETOBACTER DIAZOTROPHICUS</text>
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                <text>RALSTONIA</text>
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                <text>BIBLIOGRAFIA NACIONAL QUIMICA</text>
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                <text>2019</text>
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                <text>Nowadays, fertilization and pest control are carried out using chemical compounds that contaminate soil and deteriorate human health. Plant growth promoting bacteria endophytes (PGPBEs), are a well-studied group of bacteria that offers benefits to the host plant, such as phytostimulation, biofertilization, and protection against other microorganisms. The study of Gluconacetobacter diazotrophicus&amp;ndash;which belongs to PGPBEs-aids the development of alternative strategies of an integrated approach for crop management practices. Ralstonia solanacearum is responsible for bacterial wilt disease. This phytopathogen is of great interest worldwide due to the enormous economic losses it causes. In this study the action of G. diazotrophicus as a growth promoting bacterium in Arabidopsis thaliana seedlings is analyzed, evaluating the antagonistic mechanisms of this beneficial endophytic bacterium during biotic stress produced by R. solanacearum. Effective colonization of G. diazotrophicus was determined through bacterial counting assays, evaluation of anatomical and growth parameters, and pigments quantification. Biocontrol assays were carried out with Ralstonia pseudosolanacearum GMI1000 model strain and R. solanacearum A21 a recently isolated strain. Inoculation of A. thaliana (Col 0) with G. diazotrophicus Pal 5 triggers a set of biochemical and structural changes in roots, stems, and leaves of seedlings. Discrete callose deposits as papillae were observed at specific sites of root hairs, trichomes, and leaf tissue. Upon R. pseudosolanacearum GMI1000 infection, endophyte-treated plants demonstrated being induced for defense through an augmented callose deposition at root hairs and leaves compared with the non-endophyte-treated controls. The endophytic bacterium appears to be able to prime callose response. Roots and stems cross sections showed that integrity of all tissues was preserved in endophyte-treated plants infected with R. solanacearum A21. The mechanisms of resistance elicited by the plant after inoculation with the endophyte would be greater lignification and sclerosis in tissues and reinforcement of the cell wall through the deposition of callose. As a consequence of this priming in plant defense response, viable phytopathogenic bacteria counting were considerably fewer in endophyte-inoculated plants than in not-inoculated controls. Our results indicate that G. diazotrophicus colonizes A. thaliana plants performing a protective role against the phytopathogenic bacterium R. solanacearum promoting</text>
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                <text>&lt;strong&gt;Rodr&amp;iacute;guez, Mar&amp;iacute;a V.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Tano, Josefina&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Ansaldi, Nazarena&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Carrau, Anal&amp;iacute;a&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Srebot, Mar&amp;iacute;a S.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Ferreira, Virginia&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Mart&amp;iacute;nez, Mar&amp;iacute;a L.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Cortadi, Adriana A.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Siri, Mar&amp;iacute;a In&amp;eacute;s&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Orellano, Elena G.&lt;/strong&gt;</text>
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                <text>Frontiers in Plant Science v. 10, 2019. -- p. 1-20.--e1618</text>
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              <elementText elementTextId="546485">
                <text>Frontiers Media</text>
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            <name>Date</name>
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                <text>2019</text>
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            <name>Rights</name>
            <description>Information about rights held in and over the resource</description>
            <elementTextContainer>
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                <text>&lt;strong&gt;Isolation and Characterization of Melanoidins from Dulce de Leche, A Confectionary Dairy Product&lt;/strong&gt;</text>
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                <text>Melanoidins, the brown-colored compounds formed through the Maillard reaction, are responsible for color development in dulce de leche (DL), a popular confectionary dairy product in the R&amp;iacute;o de la Plata region, particularly in Uruguay and Argentina. Color is a critical quality parameter that strongly influences consumer preference. This work aimed to develop a method to perform preliminary structural characterization of the chromophores produced by the Maillard reaction. Melanoidins are present in a water-insoluble fraction, linked to a protein backbone, conforming melanoproteins of high molecular weight. The insoluble melanoprotein fraction (10% total solids) was isolated, and the chromophores released by proteolysis and isolated by gel-permeation chromatography. The analysis of the products revealed that they present a high degree of molecular weight (MW) polydispersity, in a range of 300 to 2000 Da, where the compounds of higher molecular weight contributed the most to the color of the product. The isolated fractions were further analyzed by RP-HPLC using a diode array detector (DAD) detector. These results, together with H-NMR data, suggested that the chromophores isolated belonged to a relatively simple mixture of aromatic products with higher hydrophobic character relative to other products of the melanoprotein digestion.</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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                <text>The first total synthesis of cyclotetrapeptide versicotide D has been achieved in 21% overall yield using solid phase peptide synthesis and solution cyclization. In addition, in the search for candidates of antimalarial new drugs, one cyclic tetrapeptide analog which differs in the sequence, and four cyclic pentapeptide containing N-methyl amino acids, were prepared. The obtained compounds were evaluated against P. falciparum 3D7. Versicotide D showed low micromolar antiplasmodial activity.</text>
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&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Particles from the Echinococcus granulosus Laminated Layer Inhibit CD40 Upregulation in Dendritic Cells by Interfering with Akt Activation&lt;/strong&gt;</text>
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                <text>The larval stage of the cestode Echinococcus granulosus causes cystic echinococcosis in humans and livestock. This larva is protected by the millimeter-thick, mucin-based laminated layer (LL), from which materials have to be shed to allow parasite growth. We previously reported that dendritic cells (DCs) respond to microscopic pieces of the mucin gel of the LL (pLL) with unconventional maturation phenotypes, in the absence or presence of Toll-like receptor (TLR) agonists, including lipopolysaccharide (LPS). We also reported that the presence of pLL inhibited the activating phosphorylation of the phosphatidylinositol 3-kinase (PI3K) effector Akt induced by granulocyte-macrophage colony-stimulating factor or interleukin-4. We now show that the inhibitory effect of pLL extends to LPS as a PI3K activator, and results in diminished phosphorylation of GSK3 downstream from Akt. Functionally, the inhibition of Akt and GSK3 phosphorylation are linked to the blunted upregulation of CD40, a major feature of the unconventional maturation phenotype. Paradoxically, all aspects of unconventional maturation induced by pLL depend on PI3K class I. Additional components of the phagocytic machinery are needed, but phagocytosis of pLL particles is not required. These observations hint at a DC response mechanism related to receptor-independent mechanisms proposed for certain crystalline and synthetic polymer-based particles; this would fit the previously reported lack of detection of molecular-level motifs necessary of the effects of pLL on DCs. Finally, we report that DCs exposed to pLL are able to condition DCs not exposed to the material so that these cannot upregulate CD40 in full in response to LPS.</text>
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                <text>&lt;strong&gt;Pittini, &amp;Aacute;lvaro.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Yamila E. Mart&amp;iacute;nez Acosta&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Casaravilla, Cecilia&lt;/strong&gt;</text>
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                <text>Infection and Immunity v. 87, no. 12, 2019. -- p. 1-19.--e00641-19</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Ultrasonication is known to induce cavitation and can thus accelerate extraction, dissolution, digestion, and other processes, while the intense mixing caused by the propagation of ultrasound waves enhances analyte transfer to the extraction medium. Consequently, ultrasound-assisted extraction (UAE) is viewed as a simple procedure well suited for the extraction and subsequent determination of trace elements in food. Herein, we use different techniques to characterize several processes involved in probe- and bath-based UAE, apply them to the determination of trace metals (Cd, Cu, Ni, Pb, and Zn) in globe artichoke leaves and soybean seeds, and closely monitor the extraction rates of selected analytes. The developed UAE-based method is found to comply with the criteria of Green Chemistry and is concluded to be a reliable, simple, and cheap alternative to standard food analysis techniques.</text>
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                <text>The heteroatom doping of graphene is a useful method to amplify the outstanding properties of graphene. However, it is difficult to control the disposition of the dopants. By means of first-principles calculations, we show that the substrate-induced doping of epitaxial graphene leads to the formation of silicon doped graphene with a precise location of the dopants in the valley regions between crests. According to our results, in the valleys silicon dopants greatly prefer to replace the carbon atoms of the buffer layer which are bonded to the substrate by a large amount of energy. This selective doping suggests that a siligraphene layer in the g-SiC3 form would be an excellent model for a buffer layer grown on nanometer-sized areas of SiC, thus paving the way to the synthesis of a new two-dimensional material. We predict a stable antiferromagnetical (AF) ordering that behaves electronically as a semiconductor, degenerated with the highest ferromagnetic configuration of the system. A net magnetic moment of 0.65 &amp;mu;B per Si dangling bond was found for both the AF and the most stable ferromagnetic state of the SiC-siligraphene system. Finally, we demonstrate that quasi-free-standing epitaxial siligraphene layers on 6H-SiC(0001) could be obtained by the intercalation of argon atoms. We expect that this work can motivate new experimental studies pursuing the synthesis of SiC3 siligraphene.</text>
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                <text>Helminths use an alternative mitochondrial electron transport chain (ETC) under hypoxic conditions, such as those found in the gastrointestinal tract. In this alternative ETC, fumarate is the final electron acceptor and rhodoquinone (RQ) serves as an electron carrier. RQ receives electrons from reduced nicotinamide adenine dinucleotide through complex I and donates electrons to fumarate through complex II. In this latter reaction, complex II functions in the opposite direction to the conventional ETC (i.e., as fumarate reductase instead of succinate dehydrogenase). Studies in Ascaris suum indicate that this is possible due to changes in complex II, involving alternative succinate dehydrogenase (SDH) subunits SDHA and SDHD, derived from duplicated genes. We analyzed helminth genomes and found that distinct lineages have different gene duplications of complex II subunits (SDHA, SDHB, SDHC, and SDHD). Similarly, we found lineage-specific duplications in genes encoding complex I subunits that interact with quinones (NDUF2 and NDUF7). The phylogenetic analysis of ETC subunits revealed a complex history with independent evolutionary events involving gene duplications and losses. Our results indicated that there is not a common evolutionary event related to ETC subunit genes linked to RQ. The free-living nematode Caenorhabditis elegans uses RQ and has two genes encoding SDHA (sdha-1 and sdha-2) and two genes encoding NDUF2 (nduf2-1 and nduf2-2). sdha-1 and nduf2-1 are essential genes and have a similar expression pattern during C. elegans lifecycle. Using knockout strains, we found that sdha-2 and nduf2-2 are not essential, even in hypoxia. Yet, sdha-2 and nduf2-2 expression is increased in the early embryo and in dauer larvae, stages where there is low oxygen tension. Strikingly, sdha-1 and sdha-2 as well as nduf2-1 and nduf2-2 showed inverted expression profiles during the C. elegans life cycle. Finally, we found that sdha-2 and nduf2-2 knockout mutant strain progeny is affected. Our results indicate that different complex I and II subunit gene duplications provide increased fitness to worms.</text>
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                <text>Frontiers in Genetics v.10, 2019.--p. 1-10.--e1043</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre&lt;/p&gt;</text>
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                <text>Microbial biodiversity and its rich arsenal of natural products is an important and complex biological concept. We propose the use of Streptomyces, one of the most diverse bacterial genera and a major inhabitant of soil, as a model to explain the relevance and importance of this concept. Students will perform experiments ranging from isolation and selection of Streptomyces species, to performing fungal and bacterial challenge assays to evaluate their biocontrol capacity, to screening for specialized metabolic properties such as the production of lipases, amylases, and cellulases. Accompanied by active discussions on the experimental process and results, and integrated into the general microbiology curriculum, this real-life discovery-based lab exercise engages students in current topics concerning natural product discovery and reinforces their understanding of several important concepts in microbiology and biotechnology</text>
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                <text>&lt;strong&gt;Lapaz, Mar&amp;iacute;a In&amp;eacute;s&lt;/strong&gt;</text>
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                <text>American Biology Teacher v. 81, no. 9, 2019. --p. 658-664</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora&lt;/p&gt;</text>
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                <text>Echinococcus granulosus is a cestode parasite whose cytoskeleton plasticity allows it to enter and develop inside its hosts, completing thus its life cycle. We focused our attention on F-actin organization and distribution in E. granulosus protoscoleces (PSC) in order to contribute to the knowledge of the parasite cytoskeleton. In particular, we addressed some aspects of F-actin rearrangements in PSC at different stages of the evagination/invagination process. The use of light microscopy allowed us to identify different PSC structures and phalloidin staining displayed a parasite's highly organized F-actin cytoskeleton. Suckers exhibit an important musculature composed of a set of radial fibers. At the rostellum, the F-actin filaments are arranged in a bulbar shape with perforations that appear to be the attachment places for the hooks. Also, "circular" structures of F-actin were identified, which remind the flame cells. Furthermore, parasite F-actin filaments, unevenly distributed, seem to have remained substantially unchanged during the evagination/invagination process. Finally, we showed that the scolex of an evaginated E. granulosus PSC reinvaginates in vitro without any treatment.</text>
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                <text>Acta Tropica v. 199, 2019.--p. 1-7.--e105122</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>DOI:10.1016/j.actatropica.2019.105122</text>
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                  <text>Bibliografía Nacional Química</text>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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                <text>&lt;strong&gt;Effectiveness of micronizing zinc borate to improve its fungicidal properties&lt;/strong&gt;</text>
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                <text>FUNGICIDAS</text>
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                <text>BORATO DE ZINC</text>
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                <text>LIXIVIACION</text>
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                <text>The effectiveness was evaluated for an established wood preservative, zinc borate, prepared in an agitating bead mill to extensively reduce its particle size. The generated micro- or nano-particles of zinc borate were characterized by X-ray powder diffraction, and the particle size distribution was determined to evaluate the effect of milling. Then the fungicidal effectiveness of the zinc borate of both milled and unmilled samples were assayed against brown- and white-rot fungi, on culture medium and on conifer and hardwood as substrates. Treated wood samples were subjected to leaching tests. Scanning electron microscopic images of wood samples were examined to analyze the distribution of zinc borate within the wood. The micronized zinc product kept its crystal structure intact, and it increased the proportion of particles with diameters below 100 nm by 25% when compared to the unmilled product. Malt extract-agar medium supplemented with 2.5% of w/w milled and unmilled zinc borate inhibited fungal growth tested. Both milled and unmilled zinc borate protected the wood when not subjected to leaching. The milled sample of zinc borate improved resistance to leaching, which would allow its application in environments of high moisture content; however, it did not improve the fungicidal action against decay fungi.</text>
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                <text>&lt;strong&gt;Iba&amp;ntilde;ez, Claudia M.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Camargo, Alvaro&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Mantero, Carlos&lt;/strong&gt;</text>
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                <text>&lt;a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=2f2e59581473324400ee29ded741b9090cc25a893e73d02669ebfbc674c9e8b9b51bb30e2bca064dc7ebbd5c39aa3c844e86e507301e912aa1b0a5ae17423fa2&amp;amp;formato=pdf&amp;amp;convocatoria=21" target="_self"&gt;&lt;strong&gt;Faccio, Ricardo.&lt;/strong&gt;&lt;/a&gt;</text>
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                <text>&lt;strong&gt;Malanga, Antonio&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Rabinovich, Mario&lt;/strong&gt;</text>
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                <text>BioResources&amp;nbsp; v. 14, no. 3, 2019.-- p. 6231-6246</text>
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              <elementText elementTextId="545890">
                <text>NC State University.</text>
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              <elementText elementTextId="545892">
                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>The capacity of microorganisms from water kefir (WK) to control Aspergillus flavus growth during the aerobic phase of ensiled sorghum grains was determined. Sorghum inoculated with A. flavus was treated with filter-sterilized and non-sterilized water kefir, ensiled, and incubated 7 days at 25 &amp;deg;C. A. flavus growth was quantified by qPCR after incubation. Mold growth was inhibited in the presence of water kefir while no inhibition was observed when filter-sterilized water kefir was applied, demonstrating the relevant role of the microorganisms in the kefir water in the biocontrol process. Fungal and bacterial diversity in treated sorghum mini-silos was analyzed by high-throughput sequencing. Firmicutes was the predominant bacterial phyla and Lactobacillus represented the most abundant genus, while Ascomycota was the predominant fungal phyla with Saccharomyces and Pichia as the major genera. Bacterial and yeast counts before and after incubation indicated that the microbial community obtained from WK was able to grow in the sorghum mini-silos in the presence of A. flavus. Results of the present work indicate that the use of a mixed inoculum of microorganisms present in WK may represent an alternative management practice to avoid the growth of A. flavus in ensiled sorghum grains and the concomitant contamination with aflatoxins.</text>
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                <text>&lt;strong&gt;Gonda, Mariana.&lt;/strong&gt;</text>
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                <text>Microorganisms v. 7, no. 8, 2019. -- p. 1-18.--e253</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>DOI: 10.3390/microorganisms7080253</text>
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                  <text>Bibliografía Nacional Química</text>
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                  <text>Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.</text>
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                <text>&lt;strong&gt;Genomic and Transcriptomic Basis of Hanseniaspora vineae&amp;rsquo;s Impact on Flavor Diversity and Wine Quality&lt;/strong&gt;</text>
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                <text>Hanseniaspora is the main genus of the apiculate yeast group that represents approximately 70% of the grape-associated microflora. Hanseniaspora vineae is emerging as a promising species for quality wine production compared to other non-Saccharomyces species. Wines produced by H. vineae with Saccharomyces cerevisiae consistently exhibit more intense fruity flavors and complexity than wines produced by S. cerevisiae alone. In this work, genome sequencing, assembling, and phylogenetic analysis of two strains of H. vineae showed that it is a member of the Saccharomyces complex and it diverged before the whole-genome duplication (WGD) event from this clade. Specific flavor gene duplications and absences were identified in the H. vineae genome compared to 14 fully sequenced industrial S. cerevisiae genomes. The increased formation of 2-phenylethyl acetate and phenylpropanoids such as 2-phenylethyl and benzyl alcohols might be explained by gene duplications of H. vineae aromatic amino acid aminotransferases (ARO8 and ARO9) and phenylpyruvate decarboxylases (ARO10). Transcriptome and aroma profiles under fermentation conditions confirmed these genes were highly expressed at the beginning of stationary phase coupled to the production of their related compounds. The extremely high level of acetate esters produced by H. vineae compared to that by S. cerevisiae is consistent with the identification of six novel proteins with alcohol acetyltransferase (AATase) domains. The absence of the branched-chain amino acid transaminases (BAT2) and acyl coenzyme A (acyl-CoA)/ethanol O-acyltransferases (EEB1) genes correlates with H. vineae's reduced production of branched-chain higher alcohols, fatty acids, and ethyl esters, respectively. Our study provides sustenance for understanding and potentially utilizing genes that determine fermentation aromas.IMPORTANCE The huge diversity of non-Saccharomyces yeasts in grapes is dominated by the apiculate genus Hanseniaspora Two native strains of Hanseniaspora vineae applied to winemaking because of their high oenological potential in aroma and fermentation performance were selected to obtain high-quality genomes. Here, we present a phylogenetic analysis and the complete transcriptome and aroma metabolome of H. vineae during three fermentation steps. This species produced significantly richer flavor compound diversity than Saccharomyces, including benzenoids, phenylpropanoids, and acetate-derived compounds. The identification of six proteins, different from S. cerevisiae ATF, with diverse acetyltransferase domains in H. vineae offers a relevant source of native genetic variants for this enzymatic activity. The discovery of benzenoid synthesis capacity in H. vineae provides a new eukaryotic model to dilucidate an alternative pathway to that catalyzed by plants' phenylalanine lyases.</text>
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                <text>&lt;strong&gt;Giorello, Facundo&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Salzman, Valentina&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Camesasca, Laura&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Aguilar, Pablo S.&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Gaggero de Munno, Carina.&lt;/strong&gt;</text>
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                <text>Applied and Environmental Microbiology v. 85, no. 1, 2019. -- p. 1-20. --e01959-18</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA&lt;/strong&gt; - La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;99mTc labelling strategies for the development of potential nitroimidazolic hypoxia imaging agents&lt;/strong&gt;</text>
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                <text>Technetium-99m has a rich coordination chemistry that offers many possibilities in terms of oxidation states and donor atom sets. Modifications in the structure of the technetium complexes could be very useful for fine tuning the physicochemical and biological properties of potential 99mTc radiopharmaceuticals. However, systematic study of the influence of the labelling strategy on the &amp;ldquo;in vitro&amp;rdquo; and &amp;ldquo;in vivo&amp;rdquo; behaviour is necessary for a rational design of radiopharmaceuticals. Herein we present a review of the influence of the Tc complexes&amp;rsquo; molecular structure on the biodistribution and the interaction with the biological target of potential nitroimidazolic hypoxia imaging radiopharmaceuticals presented in the literature from 2010 to the present. Comparison with the gold standard [18F]Fluoromisonidazole (FMISO) is also presented</text>
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                <text>Inorganics v. 7, no. 11, 2019.-- p. 1-16.--e7110128</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>&lt;strong&gt;Theoretical investigation of various aspects of two dimensional holey boroxine, B3O3&lt;/strong&gt;</text>
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                <text>By means of first-principles calculations, we study the structural, electronic and mechanical properties of the newly synthesized boron&amp;ndash;oxygen holey framework (Chem. Comm. 2018, 54, 3971). It has a planar structure formed by B3O3 hexagons, which are joined via strong covalent boron&amp;ndash;boron bonds. The six B3O3 units are connected with six-fold symmetry exhibiting a large hole with a surface area of 23 &amp;Aring;2, which is ideal for the adsorption of alkalis. For neutral alkalis, we found that the adsorption energy of potassium is 14 and 12 kcal mol&amp;minus;1 larger than those determined for sodium and lithium, respectively. In contrast, for alkali cations, there is a clear preference for lithium over sodium and potassium. With regard to its electronic properties, it is an insulator with an electronic band gap of 5.3 eV, at the HSE level of theory. We further investigate the effect of strain on the band gap and find it a less efficient technique to tune the electronic properties. The wide optical gap of B3O3 can be utilized in ultraviolet (UV) applications, such as UV photodetectors, etc. Additionally, the 2D elastic modulus of B3O3 (53.9 N m&amp;minus;1) is larger than that of Be3N2, silicene, and germanene. Besides, we also report bilayer and graphite-like bulk B3O3 and furthermore, find that the optoelectronic properties of the bilayer can be tuned with an external electric field. The great tunability of optical properties from UV to the visible range offers a vast range of applications in optoelectronics.</text>
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                <text>RSC Advances v. 9, no. 64, 2019. -- p. 37526-37536</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona&lt;/p&gt;</text>
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                <text>Enantioselective synthesis of ampelomin B and epi-ampelomin B, D, and E was accomplished starting from toluene, through a chemoenzymatic sequence, in which stereoselective hydrogenation, Mitsunobu reaction, and regio- and stereoselective nucleophilic opening of an epoxide were used as the main transformations. Structural revision and absolute configuration of the natural compounds were carried out.</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya&lt;/strong&gt; protege el derecho de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse&lt;/p&gt;</text>
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