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                <text>&lt;strong&gt;Raman Microscopy Insights on the Out-of-Plane Electrical Transport of Carbon Nanotube-Doped PEDOT : PSS Electrodes for Solar Cell Applications&lt;/strong&gt;</text>
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                <text>In the present report, we focused on the study of the out-of-plane electrical transport of multiwalled carbon nanotube (MWCNT)-doped poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) composites (PEDOT:PSS-MWCNTs) as electrodes for solar cell applications. The out-of-plane direct current and alternating current electrical transport, rarely studied but not less relevant, was additionally supported with in-plane and out-of-plane confocal Raman microscopy and grazing incidence small-angle X-ray scattering characterizations. The main relevance of our study is the monitoring of the polymer structure all across the polymeric film by using confocal Raman spectroscopy and its correlation with electrical transport. Modifications in the PEDOT benzenoid and quinoid conformations were observed in the vicinities of MWCNTs, and the enrichment of PSS at the indium tin oxide electrode interface was also evidenced. In consequence, the low MWCNT loadings into PEDOT:PSS lead to an increase of the out-of-plane conductivity, but the heavier MWCNT loadings lead to a drastic decrease. The tuning of the doping level of these polymer composites and the understanding of the interface structure are crucial to fabricate electrodes with higher out-of-plane conductivities for organic solar cell applications.</text>
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                <text>&lt;strong&gt;Mombr&amp;uacute;, Dominique&lt;/strong&gt;</text>
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                <text>Journal of Physical Chemistry B&amp;nbsp; v. 122, 2018. -- p. 2694-2701</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>DOI: 10.1021/acs.jpcb.8b00317</text>
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                <text>While phenolic compounds can impact the sensory characteristics of quality wines, the chemical modifications induced by yeasts on phenolic compounds have been studied only to a limited extent. Forty-nine non-Saccharomyces yeast strains, belonging to different genera, were screened in a synthetic must supplemented with anthocyanins from Tannat grapes, for their effects on color and fermentation capability. Total anthocyanins, color intensity, hue and total polyphenol index were significantly affected (p &amp;lt; 0.001) by the different yeasts studied. The effects on these parameters were highly diverse depending on the yeast species studied according to their capacities of improving color indexes of the red grape model wines. Six strains belonging to the species Metschnikowia pulcherrima, Hanseniaspora guilliermondii, H. opuntiae, H. vineae and H. clermontiae, were selected in order to evaluate the production of anthocyanin-derived pigments, and significant variations in the production of four compounds were found between them and compared to Saccharomyces cerevisiae. The formation of vitisin A, vitisin B, malvidine-3-glucoside-4-vinylphenol and malvidin-3-glucoside-4-vinylguaiacol are reported for the first time for species of genera Hanseniaspora and Metschnikowia.</text>
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                <text>&lt;strong&gt;Medina, Karina&lt;/strong&gt;</text>
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                <text>American Journal of Enology and Viticulture v. 69, no. 2, 2018. -- p. 148-156</text>
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                <text>American Society for Enology and Viticulture</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Apples, one of the most popular fruit worldwide, are globally traded and consumers benefit from hyper-competition in the industry. To remain profitable, producers must offer very high quality fruit at competitive prices. This research sought to better understand consumers&amp;rsquo; quality perceptions. We focused on external appearance, a readily available quality cue. Insights were gained from a categorization task where participants sorted images of apples based on perceived quality. A comparison between experts&amp;rsquo; (n&amp;thinsp;=&amp;thinsp;37) and consumers&amp;rsquo; (n&amp;thinsp;=&amp;thinsp;297) quality perceptions based on external appearance revealed high similarity. Both groups considered fruit with minor defects as inferior to fruit with no external defects. The consumers, who represented four different countries (72&amp;ndash;76 per country; loosely regarded as a proxy for the global population of apple consumers), perceived the quality of the apples very similarly. Three quality groupings were established, representing apples with high quality, minor defects and major defects, respectively. The last-mentioned grouping consisted of any apple that showed signs of rot or mold, or where there the skin had been cut. Blemishes and odd shapes were generally regarded as minor defects. Both types of defects were associated with negative hedonic expectations, which increased with the severity of the external defect. The implications for consumers&amp;rsquo; purchase-related cognitions were clear and associated with rejecting consumption. This has associated implications for efforts to reduce food waste.</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Oryzanols are natural antioxidants that are found in appreciable amounts in rice bran oil. However, by chemically refining the crude rice bran oil they are lost during the chemical neutralization step leaving the oil refined with very little oryzanols. The chemical neutralization leaves a residue called "soapstocks" where most of these antioxidants are found. From the soapstocks and by relatively simple procedures it is possible to obtain a oryzanols concentrate which may contain 33% of them. However, its antioxidant power has been little studied in oils compared to other natural antioxidants. Therefore, the present work gives information about the antioxidant power of a concentrate of oryzanols compared to natural antioxidants such as tocopherol and synthetic antioxidants such as butylhydroxytoluene (BHT) added in oils with different degrees of unsaturation and without antioxidants. The results determined by the differential scanning calorimetry method show that the antioxidant power was variable according to the method used. The tocopherol protected the oils from the oxidation at 130&amp;deg;C (soybean and high oleic sunflower) better than the oryzanols concentrate by the isothermal method. When the non-isothermal method was used it was found that the-tocopherol protected soybean oil better than oleic high sunflower oil compared to the oryzanols concentrate. However, when comparing BHT with oryzanols concentrate, BHT generally had a lower protection in both oils and both methods. These results show that the oryzanols concentrate has a protective effect of the oxidation of the studied oils, however, this could depend on the degree of the oil unsaturation.</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Process optimization for producing diacylglycerol-enriched oils has received great attention due the importance of these products for healthier and more balanced diets. This work reports experimental data of liquid-liquid phase equilibrium of hydrolyzed olive oil (which comprises on its composition triacylglycerols, diacylglycerols, monoacylglycerols and free fatty acids) with ethanol and water, and subsequent analysis of oxidative stability of the oil obtained from the liquid phases. Liquid-liquid equilibrium experimental data obtained were modeled using the UNIQUAC thermodynamic model, which was well fitted to experimental data with a root mean square deviation of 1.76 wt%. The results obtained demonstrated the potentiality of liquid-liquid extraction as a suitable procedure for olive oil enrichment in diacylglycerol, after a hydrolysis procedure. It was also observed that liquid-liquid phase separation has a significant effect on the content of important antioxidants present in the olive oil, what affects the oxidative stability of the samples submitted to the liquid phase extraction. From the results obtained in this study, it has been also noticed that the acylglycerols and free fatty acids profile in of the oil samples presented a major rule on their stabilities.</text>
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                <text>Journal of Food Engineering&amp;nbsp; v. 233, 2018. -- p. 1-8</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>Glutathione transferase enzymes (GSTs) constitute a major detoxification system in helminth parasites and have been related to the modulation of host immune response mechanisms. At least three different GSTs classes have been described in Platyhelminthes: Mu, Sigma and Omega. Mining the genome of Echinococcus multilocularis and the ESTs databases of Taenia solium and E. granulosus identified two new GSTs from the cestode E. granulosus, named EgGST2 and EgGST3. It also revealed that the Omega class of GSTs was absent from the Taenidae family. EgGST2 and EgGST3 are actively expressed in the parasite. In order to know the origin of these new GSTs, in silico analyses were performed. While EgGST2 is classified as belonging to the Sigma class, the data obtained for EgGST3 allowed a less clear interpretation. The study of the evolutionary relatedness based on the C-terminal domain sequence, gene structure conservation and three-dimensional structure predictions, suggests that EgGST3 is derived from the Platyhelminthes' Sigma-class cluster. Interestingly, the N-terminal domain displays some characteristic Omega-class residues, including a Cys residue that is likely to be involved in the catalytic mechanism. We discuss different evolutionary scenarios that could explain the observed patterns.</text>
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                <text>&lt;strong&gt;Arbildi Torres, Paula.&lt;/strong&gt;</text>
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                <text>Acta Tropica v. 123, 2012. -- p. 208-216</text>
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&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>The use of H/D isotope effects on nuclear shielding (IENS) as a tool to investigate interionic hydrogen bonds (H-bonds) in ionic liquids (ILs) is showcased. Results obtained with deuterated isotopologues of 1-n-butyl-3-methylimidazolium chloride ([C4mim]Cl), hexafluorofosphate ([C4mim]PF6), and tetrafluroborate ([C4mim]BF4) indicate that the H/D IENS on the 35/37Cl and 19F resonances of the anions, or &amp;Delta;35/37Cl(H,D) and &amp;Delta;19F(H,D), allow to identify and differentiate H-bonds between the anion and the different aromatic protons on the cation. H/D IENS for sequentially deuterated [C4mim]BF4 and [C4mim]PF6 isotopologues also reveal the formation of aliphatic C-H&amp;sdot;&amp;sdot;&amp;sdot;X hydrogen bonds between the fluorinated anions and protons along the sidechains, and indicate that the interactions between the anions and hydrogens on the N-alkyl C-1&amp;rsquo; and C-1&amp;rdquo; positions are, in some cases, stronger than those involving protons on the aromatic ring. Preliminary studies aimed at determining the effects of sidechain length and structure on intrerionic H-bonds in ILs are also discussed.</text>
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                <text>ECS Transactions v. 86, 2018. -- p. 179-185</text>
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                <text>&lt;strong&gt;Determining the relative strengths of aromatic and aliphatic C―H X hydrogen bonds in imidazolium ionic liquids through measurement of H/D isotope effects on 19F nuclear shielding&lt;/strong&gt;</text>
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                <text>The relative strengths of aromatic and aliphatic C―H&amp;sdot;&amp;sdot;&amp;sdot;X hydrogen bonds in imidazolium ionic liquids were investigated through measurement of H/D isotope effects on the 19F nuclear shielding of deuterated isotopologues of 1‐n‐butyl‐3‐methylimidazolium hexafluorophosphate and tetrafluoroborate ([C4mim]PF6 and [C4mim]BF4). &amp;Delta;19F(H,D) values ranging from 9.7 to 49.7 ppb were observed for [C4mim]PF6 isotopologues, while for the [C4mim]BF4 series these went from 26.2 to 83.8 ppb. Our findings indicate that the interactions between the fluorinated anions and protons on the C‐1&amp;prime; and C‐1&amp;Prime; position of the N‐alkyl sidechains are comparable to, and in some cases stronger than, those involving protons on the aromatic ring, underscoring the role that these weak interionic forces have on the local ordering of imidazolium salts in the liquid state.</text>
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                <text>&lt;strong&gt;Bottini, Gualberto&lt;/strong&gt;</text>
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                <text>Magnetic Resonance in Chemistry v. 56, 2018.-- p. 103-107</text>
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&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
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                <text>&lt;strong&gt;The Structure of the Lipid A from the Halophilic Bacterium Spiribacter salinus M19-40T&lt;/strong&gt;</text>
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                <text> MICROBIOLOGIA</text>
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                <text>BACTERIAS GRAM NEGATIVAS</text>
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                <text>LIPOPOLISACARIDOS</text>
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                <text>BIBLIOGRAFIA NACIONAL QUIMICA</text>
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                <text>The study of the adaptation mechanisms that allow microorganisms to live and proliferate in an extreme habitat is a growing research field. Directly exposed to the external environment, lipopolysaccharides (LPS) from Gram-negative bacteria are of great appeal as they can present particular structural features that may aid the understanding of the adaptation processes. Moreover, through being involved in modulating the mammalian immune system response in a structure-dependent fashion, the elucidation of the LPS structure can also be seen as a fundamental step from a biomedical point of view. In this paper, the lipid A structure of the LPS from Spiribacter salinus M19-40T, a halophilic gamma-proteobacteria, was characterized through chemical analyses and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. This revealed a mixture of mono- and bisphosphorylated penta- to tri-acylated species with the uncommon 2 + 3 symmetry and bearing an unusual 3-oxotetradecaonic acid.</text>
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                <text>&lt;strong&gt;Barrau, Clara&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Di Lorenzo, Flaviana&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Menes, Rodolfo Javier&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Lanzetta, Rosa&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;Molinaro, Antonio&lt;/strong&gt;</text>
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                <text>Marine Drugs v. 16, 2018. -- p. 1-11.--e16040124</text>
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                <text>MDPI</text>
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                <text>2018</text>
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            <name>Rights</name>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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                <text>PDF</text>
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                <text>Ingl&amp;egrave;s</text>
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                <text>DOI: 10.3390/md16040124</text>
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          <name>Original Format</name>
          <description>If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data</description>
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            <name>Title</name>
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                <text>&lt;strong&gt;Producci&amp;oacute;n de lactasa por fermentaci&amp;oacute;n partiendo de c&amp;aacute;ndida&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;LACTASA&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;BETA GALACTOSIDASA&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;PRODUCCION&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;FERMENTACION&lt;/strong&gt;</text>
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                <text>&lt;strong&gt;CANDIDA&lt;/strong&gt;</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="484814">
                <text>Biblioteca-FQ</text>
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            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>1985</text>
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            <name>Format</name>
            <description>The file format, physical medium, or dimensions of the resource</description>
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              <elementText elementTextId="484816">
                <text>Papel</text>
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                <text>PDF</text>
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            <name>Language</name>
            <description>A language of the resource</description>
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                <text>Ingl&amp;eacute;s</text>
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                <text>Bibliograf&amp;iacute;a</text>
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                <text>1970-1979</text>
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        <name>Beta galactosidasa</name>
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        <name>Cándida</name>
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        <name>Fermentación</name>
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        <name>Lactasa</name>
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        <name>Producción</name>
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          <name>Original Format</name>
          <description>If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data</description>
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            <name>Title</name>
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                <text>&lt;strong&gt;Inmunodetecci&amp;oacute;n de peque&amp;ntilde;os analitos con nannopept&amp;aacute;meros&lt;/strong&gt;</text>
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                <text>La utilizaci&amp;oacute;n de p&amp;eacute;ptidos anti-inmunocomplejos para la detecci&amp;oacute;n no competitiva de peque&amp;ntilde;as mol&amp;eacute;culas ha mostrado ser una alternativa ventajosa respecto a los formatos competitivos, dado que com&amp;uacute;nmente aumentan la sensibilidad y especificidad, y generan una se&amp;ntilde;al proporcional y por tanto intuitiva. Inicialmente, este principio fue demostrado en formato PHAIA (Phage anti-immunocomplex assay) utilizando p&amp;eacute;ptidos expresados en la superficie de part&amp;iacute;culas virales. Posteriormente, con el fin de generar reactivos m&amp;aacute;s atractivos para la industria de inmunoensayos, se montaron ensayos de igual desempe&amp;ntilde;o, pero sustituyendo los fagos por p&amp;eacute;ptidos anti-inmunocomplejos biotinilados unidos a la estreptavidina, que llamamos nanopept&amp;aacute;meros. Este trabajo de tesis transcurri&amp;oacute; dentro de este marco de antecedentes, teniendo como principales objetivos simplificar la producci&amp;oacute;n de los reactivos utilizados en los ensayos no competitivos y explorar variantes de estos ensayos para potenciar a&amp;uacute;n m&amp;aacute;s su desempe&amp;ntilde;o como herramientas para monitoreo de analitos peque&amp;ntilde;os. La primera parte de esta tesis se centr&amp;oacute; en la producci&amp;oacute;n de nanopept&amp;aacute;meros en forma recombinante, realizando la expresi&amp;oacute;n en E. coli de p&amp;eacute;ptidos fusionados a prote&amp;iacute;nas multim&amp;eacute;ricas, como estreptavidina y la verotoxina. Todos los nanopept&amp;aacute;meros producidos fueron funcionales y permitieron la detecci&amp;oacute;n de los analitos modelo, clomazone y molinato, alcanzando l&amp;iacute;mites de detecci&amp;oacute;n equivalentes a los obtenidos con PHAIA, y con el potencial de ser aplicados en formatos de tiras de diagn&amp;oacute;stico para detecci&amp;oacute;n r&amp;aacute;pida e in-situ. Como resultado de lo anterior se estableci&amp;oacute; una plataforma tecnol&amp;oacute;gica dedicada a la detecci&amp;oacute;n de peque&amp;ntilde;as mol&amp;eacute;culas y que consiste en una instancia de selecci&amp;oacute;n de p&amp;eacute;ptidos anti-inmunocomplejos con evaluaci&amp;oacute;n de su desempe&amp;ntilde;o en PHAIA, seguido de la generaci&amp;oacute;n de nanopept&amp;aacute;meros, y finalmente montaje y validaci&amp;oacute;n de los inmunoensayos al analizar muestras reales. Dicha plataforma fue transferida como tal a la industria, para lo cual, en la segunda etapa de esta tesis se realiz&amp;oacute; una prueba de concepto que no se describe aqu&amp;iacute; debido a acuerdos de confidencialidad. Como alternativa al formato heterog&amp;eacute;neo en placa de ELISA, la tercera parte de este trabajo consisti&amp;oacute; en el montaje de los ensayos no competitivos en formatos homog&amp;eacute;neos (mix-and-read). La tecnolog&amp;iacute;a de ensayos homog&amp;eacute;neos, AlphaLisa, basada en la generaci&amp;oacute;n de luminiscencia mediante canalizaci&amp;oacute;n de ox&amp;iacute;geno (LOCI), fue la elegida para ser acoplada a los ensayos no competitivos. Con el herbicida atrazina como modelo se mont&amp;oacute; el inmunoensayo NanoAlphaLisa. En este sistema, el analito es reconocido por el anticuerpo inmovilizado en las microesferas (beads) aceptoras, y el inmunocomplejo as&amp;iacute; formado estabiliza su uni&amp;oacute;n a las beads dadoras cubiertas con los correspondientes nanopept&amp;aacute;meros anti-inmunocomplejo. De esta forma al irradiar las beads dadoras el ox&amp;iacute;geno singlete liberado induce la generaci&amp;oacute;n de luminiscencia en las beads aceptoras dando lugar a un ensayo con se&amp;ntilde;al proporcional a la concentraci&amp;oacute;n del analito, que fue validado con muestras de aguas superficiales. En la &amp;uacute;ltima instancia de esta tesis se apunt&amp;oacute; a complementar la tecnolog&amp;iacute;a de nanopept&amp;aacute;meros utilizando fragmentos de anticuerpos recombinantes, como alternativa a los anticuerpos convencionales aplicados en los ensayos anteriores. Utilizando coca&amp;iacute;na como analito modelo, se realizaron construcciones de bibliotecas de Fab quim&amp;eacute;ricos pollo/humano y se aisl&amp;oacute; un Fab anti-coca&amp;iacute;na. La reactividad de este Fab se caracteriz&amp;oacute; en un formato competitivo, alcanzando un l&amp;iacute;mite de detecci&amp;oacute;n inferior a los valores gu&amp;iacute;a para el control de consumo, y con reactividad despreciable por su metabolito principal, benzoilecgonina, lo cual tiene relevancia para detectar consumo reciente. Adem&amp;aacute;s de su potencial para el desarrollo de un test de detecci&amp;oacute;n de coca&amp;iacute;na en muestras de saliva, este Fab podr&amp;aacute; utilizarse para la selecci&amp;oacute;n de p&amp;eacute;ptidos anti-inmunocomplejos y la consecuente generaci&amp;oacute;n de los nanopept&amp;aacute;meros respectivos. Generar ensayos no competitivos con fragmentos de anticuerpos abrir&amp;iacute;a nuevas oportunidades de innovaci&amp;oacute;n, como por ejemplo el desarrollo de ensayos homog&amp;eacute;neos FRET como una alternativa de menor costo del NanoAlphaLisa.</text>
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                <text>&lt;a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=7ee0def1187be47e0710e877d9768b5a" target="_blank"&gt;&lt;strong&gt;Gonz&amp;aacute;lez Sapienza, Gualberto&lt;/strong&gt;&lt;/a&gt;.(director de tesis)</text>
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                <text>&lt;p&gt;&lt;strong&gt;Informaci&amp;oacute;n sobre Derechos de Autor&lt;/strong&gt;&lt;/p&gt;&#13;
&lt;p&gt;(Por favor lea este aviso antes de abrir los documentos u objetos)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt; La legislaci&amp;oacute;n uruguaya protege el derecho&lt;/strong&gt; de autor sobre toda creaci&amp;oacute;n literaria, cient&amp;iacute;fica o art&amp;iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&amp;oacute;n a lo establecido por el derecho com&amp;uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)&lt;/p&gt;&#13;
&lt;p&gt;&lt;strong&gt;ADVERTENCIA -&lt;/strong&gt; La consulta de este documento queda condicionada a la aceptaci&amp;oacute;n de las siguientes condiciones de uso: Este documento es &amp;uacute;nicamente para usos privados enmarcados en actividades de investigaci&amp;oacute;n y docencia. No se autoriza su reproducci&amp;oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&amp;oacute;n o cita de partes debe indicarse el nombre de la persona autora.&lt;/p&gt;</text>
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