<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:dcterms="http://purl.org/dc/terms/">
<rdf:Description rdf:about="https://riquim.fq.edu.uy/items/show/2301">
    <dcterms:title><![CDATA[<strong>Conformational analysis of the natural iron chelator myo-inositol 1,2,3-trisphosphate using a pyrene-based fluorescent mimic.</strong>]]></dcterms:title>
    <dcterms:subject><![CDATA[<p>ANTIOXIODANTES</p>]]></dcterms:subject>
    <dcterms:subject><![CDATA[HIERRO NATURAL]]></dcterms:subject>
    <dcterms:subject><![CDATA[CATIONES MET&Aacute;LICOS]]></dcterms:subject>
    <dcterms:subject><![CDATA[TERMODIN&Aacute;MICA]]></dcterms:subject>
    <dcterms:subject><![CDATA[CALCIO]]></dcterms:subject>
    <dcterms:subject><![CDATA[ALCOHOLES]]></dcterms:subject>
    <dcterms:subject><![CDATA[ANÁLISIS CONFORMACIONAL]]></dcterms:subject>
    <dcterms:subject><![CDATA[BIBLIOGRAFIA NACIONAL QUIMICA]]></dcterms:subject>
    <dcterms:subject><![CDATA[2010]]></dcterms:subject>
    <dcterms:abstract><![CDATA[myo-Inositol phosphates possessing the 1,2,3-trisphosphate motif share the remarkable ability to completely inhibit iron-catalysed hydroxyl radical formation. The simplest derivative, myo-inositol 1,2,3-trisphosphate [Ins(1,2,3)P3], has been proposed as an intracellular iron chelator involved in iron transport. The binding conformation of Ins(1,2,3)P3 is considered to be important to complex Fe3+ in a &lsquo;safe&rsquo; manner. Here, a pyrene-based fluorescent probe, 4,6-bispyrenoyl-myo-inositol 1,2,3,5-tetrakisphosphate [4,6-bispyrenoyl Ins(1,2,3,5)P4], has been synthesised and used to monitor the conformation of the 1,2,3-trisphosphate motif using excimer fluorescence emission. Ring-flip of the cyclohexane chair to the penta-axial conformation occurs upon association with Fe3+, evident from excimer fluorescence induced by p&ndash;p stacking of the pyrene reporter groups, accompanied by excimer formation by excitation at 351 nm. This effect is unique amongst biologically relevant metal cations, except for Ca2+ cations exceeding a 1 : 1 molar ratio. In addition, the thermodynamic constants for the interaction of the fluorescent probe with Fe3+ have been determined. The complexes formed between Fe3+ and 4,6-bispyrenoyl Ins(1,2,3,5)P4 display similar stability to those formed with Ins(1,2,3)P3, indicating that the fluorescent probe acts as a good model for the 1,2,3-trisphosphate motif. This is further supported by the antioxidant properties of 4,6-bispyrenoyl Ins(1,2,3,5)P4, which closely resemble those obtained for Ins(1,2,3)P3. The data presented confirms that Fe3+ binds tightly to the unstable penta-axial conformation of myo-inositol phosphates possessing the 1,2,3-trisphosphate motif.]]></dcterms:abstract>
    <dcterms:creator><![CDATA[<strong>Mansell, David</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Rattray, N.</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Etchells, L. L.</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Schwalbe, C. H.</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Blake, A. J.</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=b1b8a8c8b1a5835b874ac1994003c38d"><strong>Torres, Julia</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=6f16399c452f7b13f8a01a148cb38e12"><strong>Kremer, Carlos</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Bichenkova, E. V.</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Barker, C. J.</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Freeman, S.</strong>]]></dcterms:creator>
    <dcterms:source><![CDATA[Organic and Biomolecular Chemistry v. 8, no. 12, 2010. -- p. 2850-2858]]></dcterms:source>
    <dcterms:publisher><![CDATA[The Royal Society of Chemistry]]></dcterms:publisher>
    <dcterms:date><![CDATA[2010]]></dcterms:date>
    <dcterms:format><![CDATA[PDF]]></dcterms:format>
    <dcterms:language><![CDATA[Ingl&eacute;s]]></dcterms:language>
    <dcterms:type><![CDATA[Art&iacute;culo]]></dcterms:type>
    <dcterms:identifier><![CDATA[DOI: 10.1039/c001078b]]></dcterms:identifier>
</rdf:Description></rdf:RDF>
