1
25
40
-
https://riquim.fq.edu.uy/files/original/343285f630e226eca18f0641a0ec67e9.pdf
c845c1000b6329c094a98acfecd6b7ea
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
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199
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Hyperthyroidism keeps immunoglobulin levels but reduces milk fat and CD11b/c+ cells on early lactation</strong>
Subject
The topic of the resource
TIROXINA
LECHE HUMANA
LACTANCIA MATERNA
LEUCOCITOS
ANTICUERPOS
HIPERTIROIDISMO
BIBLIOGRAFIA NACIONAL QUIMICA
2024
Abstract
A summary of the resource.
Thyroid hormones influence mammary gland differentiation and lactation by binding to thyroid hormone receptors. Hyperthyroidism disrupts pregnancy and lactation, affecting offspring growth and milk production. Despite maternal milk is a vital source of bioactive compounds and nutrients for newborns, it is unclear whether hyperthyroidism alters its composition, mainly immune factors. Therefore, our work aimed to evaluate the influence of hyperthyroidism on milk quality and immunological parameters during early lactation. Twelve-weekold female Wistar rats received daily injections of 0,25 mg/kg T4 (HyperT, n = 20) or vehicle (control, n = 19) starting 8 days before mating and continuing throughout pregnancy. Rats were euthanized on day 2 of lactation for analyzing the impact of hyperthyroidism on mammary gland, serum and milk samples. HyperT pups exhibited reduced weight, length and head circumference with altered serum hormones, glucose and albumin levels. HyperT mammary gland analysis revealed structural changes, including decreased alveolar area, adipose tissue, increased connective tissue and reduced epithelial elongation, accompanied by decreased TRβ1 RNA expression. HyperT milk displayed lower caloric value and fat concentration. HyperT animals exhibited altered milk immune cell counts, displaying increased numbers of CD45+ and CD3+ cells and decreased CD11b/c+ cells without changes on milk and serum IgA, IgG and IgG2a levels. In summary, we have demonstrated that hyperthyroidism affects mammary gland morphology, disrupts pup development and alters biochemical and immunological parameters. Our findings highlight the impact of maternal hyperthyroidism on offspring early development and milk immune composition, underscoring the importance of thyroid function in maternal and neonatal immune health.
Creator
An entity primarily responsible for making the resource
<strong>Sánchez, María Belén</strong>
<strong>Michel Lara, María Cecilia</strong>
<strong>Neira, Flavia Judith</strong>
<strong>Rodríguez-Camejo, Claudio</strong>
<strong>Ríos, Juan Manuel</strong>
<strong>Viruel, Luciana Belén</strong>
<strong>Moreno-Sosa, María Tamara</strong>
<strong>Pietrobon, Elisa Olivia</strong>
<strong>Soaje, Marta</strong>
<strong>Jahn, Graciela Alma</strong>
<strong>Hernández, Ana</strong>
<strong>Valdez, Susana Ruth</strong>
<strong>Mackern-Oberti, Juan Pablo</strong>
Source
A related resource from which the described resource is derived
<span data-sheets-formula-bar-text-style="font-size:13px;color:#000000;font-weight:normal;text-decoration:none;font-family:'Arial';font-style:normal;text-decoration-skip-ink:none;">Molecular and Cellular Endocrinology, v. 594, 2024. -- e112370</span>
Publisher
An entity responsible for making the resource available
Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
2024
Rights
Information about rights held in and over the resource
<div class="element-text">
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
</div>
Format
The file format, physical medium, or dimensions of the resource
PDF
Extent
The size or duration of the resource.
13 p.
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
<span data-sheets-formula-bar-text-style="font-size:13px;color:#000000;font-weight:normal;text-decoration:none;font-family:'Arial';font-style:normal;text-decoration-skip-ink:none;">10.1016/j.mce.2024.112370</span>
Anticuerpos
Hipertiroidismo
Lactancia materna
Leche Humana
Leucocitos
Tiroxina
-
https://riquim.fq.edu.uy/files/original/71babee9121b441c0bc64c0b5c3b428a.pdf
c845c1000b6329c094a98acfecd6b7ea
https://riquim.fq.edu.uy/files/original/229a70a1ebb906b7bf2e29aa82688ade.jpg
4d4178fa9679f68a042986580d98acff
Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
155
Width
116
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Immunization with a Mu-class glutathione transferase from Echinococcus granulosus induces efficient antibody responses and confers long-term protection against secondary cystic echinococcosis</strong>
Subject
The topic of the resource
ECHINOCOCCUS GRANULOSUS
GLUTATION-S-TRANSFERASA
VACUNAS
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
2024
Abstract
A summary of the resource.
Cystic echinococcosis, a zoonosis caused by cestodes belonging to the Echinococcus granulosus sensu lato (s.l.) genetic complex, affects humans and diverse livestock species. Although a veterinary vaccine exhibiting high levels of antibody-mediated protection has successfully reached the market, the large genetic diversity among parasite isolates and their particular host preferences, makes still necessary the search for novel vaccine candidates. Glutathione transferases (GSTs) constitute attractive targets for immunoprophylaxis due to their outstanding relevance in helminth detoxification processes, against both exogenous and endogenous stressors. Among the six GSTs known to be expressed in E. granulosus s.l., EgGST1 (Mu-class), EgGST2 (Sigma-class), and EgGST3 (a still non-classifiable isoenzyme), show the highest proteomic expression. Therefore, their recombinant forms -rEgGST1, rEgGST2 and rEgGST3- were herein analyzed regarding their potential to induce long-term antiparasite protection in mice. Only immunization with rEgGST1 induced long-lasting protection; and accordingly, rEgGST1-specific antibodies enhanced the parasite killing through both the classical activation of the host complement system and the antibody-dependent cellular cytotoxicity by macrophages. These results support further testing of rEgGST1 as a vaccine candidate in diverse hosts due to the broad expression of EgGST1 in different parasite stages and tissues.
Creator
An entity primarily responsible for making the resource
<strong>Arbildi, Paula</strong>
<strong>Muniz Lagos, Ana Clara</strong>
<strong>Fernández, Eugenia</strong>
<strong>Giorgi, Rosina</strong>
<strong>Wiater, Kai</strong>
<strong>Mourglia Ettlin, Gustavo</strong>
<strong>Fernández, Verónica</strong>
Source
A related resource from which the described resource is derived
Microbes and Infection v. 26, 2024. -- e105364
Publisher
An entity responsible for making the resource available
Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
2024
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
The file format, physical medium, or dimensions of the resource
PDF
Extent
The size or duration of the resource.
9 p.
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
10.1016/j.micinf.2024.105364
Anticuerpos
Echinococcus Granulosus
Glutation-S-Transferasa
Vacunas
-
https://riquim.fq.edu.uy/files/original/298510d9a96505bad886b9b2b296b1bb.pdf
e828b1da565031351914bce432972f24
https://riquim.fq.edu.uy/files/original/ff695366ab285234fea47096845f9abf.jpg
1b8b0dd4efa50b954b7a02a0992698d8
Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
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153
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123
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Nanobody-Based blocking of binding ELISA for the detection of Anti-NS1 Zika-Virus-Specific antibodies in convalescent patients</strong>
Subject
The topic of the resource
SEROLOGIA
FLAVIVIRIDAE
INMUNOENSAYO
ANTICUERPOS
ZIKA
BIBLIOGRAFIA NACIONAL QUIMICA
2023
Abstract
A summary of the resource.
Zika virus has spread around the world with rapid pace in the last five years. Although symptoms are typically mild and unspecific, Zika’s major impact occurs during pregnancy, generating a congenital syndrome. Serology plays a key role in its diagnosis. However, its use is limited due to the uncertainty caused by the cross-reaction of antibodies elicited in response to other flavivirus infections when tested in direct immunoassays. Using a panel of previously generated anti-Zika non-structural protein 1 (NS1) nanobodies, a set was selected that only recognizes epitopes present in Zika and is immunogenic to humans. A proper arrangement of these nanobodies was made and conditions were optimized in order to develop a novel serology assay. This new ELISA relies on the inhibition of the binding of a set of selected nanobodies to Zika-immobilized NS1 when previously incubated with Zika convalescent sera. Using the developed blocking of binding assay, it was possible to discriminate between Zika-specific and cross-reactive antibodies in serum samples from infections with Zika and other flaviviruses
Creator
An entity primarily responsible for making the resource
<a href="https://export.cvuy.uy/cv/?2151545e1e2bcf5a1aed18f0fcbc86f8f572989a1ff4170a7081b84aaee68c3f0c594002567678df9a2ad53f09c41af0654f6be4d057bda841a03a8994b48e31" target="_blank"><strong>Delfin Riela, Triana</strong></a>
<a href="https://export.cvuy.uy/cvsni/?urlId=0714ab9cf73af9870ec8809b325b44115e7a8c32a7bffc5b0260762ba5347b07fa4dc1efff263fa6245889f68b031f22fc09f7dd37fb84c74004165d1dca6005&convocatoria=21&formato=html" target="_blank"><strong>Rossotti, Martín A.</strong></a>
<strong>Mattiuzzo, Giada</strong>
<strong>Echaides, César</strong>
<a href="https://export.cvuy.uy/cv/?f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20" target="_blank"><strong>González Sapienza, Gualberto</strong></a>
Source
A related resource from which the described resource is derived
Tropical Medicine and Infectious Disease, v.8, n° 1, 2023. -- e55
Publisher
An entity responsible for making the resource available
MDPI
Date
A point or period of time associated with an event in the lifecycle of the resource
2023
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
The file format, physical medium, or dimensions of the resource
PDF
Extent
The size or duration of the resource.
10 p.
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
10.3390/tropicalmed8010055
Anticuerpos
Flaviviridae
Inmunoensayo
Serología
Zika
-
https://riquim.fq.edu.uy/files/original/99b381bcac0ed318b9ecd4b202235b91.pdf
26eae7e0c2a58a43c61fe0694186c6ec
https://riquim.fq.edu.uy/files/original/f0b3bb9eaddabf1218d4df3316c48792.jpg
b038ec9c5da40cc13185e4baeafa06c4
Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
155
Width
117
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Polyreactive antibodies as potential humoral biomarkers of host resistance to cystic echinococcosis</strong>
Subject
The topic of the resource
BIOMARCADORES
EQUINOCOCOSIS
HIDATIDOSIS
ECHINOCOCCUS GRANULOSUS
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
2021
Abstract
A summary of the resource.
<p>Polyreactive antibodies (pAb) bind to a broad range of unrelated structures, providing hosts with functional components able to rapidly recognize and protect against different pathogens. However, their roles against helminth parasites are still unexplored. Here, pAb profiles were analysed in cystic echinococcosis (CE), a zoonosis caused by the cestode Echinococcus granulosus sensu lato. Levels of anti-DNP (2,4-dinitrophenyl-hapten) antibodies were measured as a surrogate parameter of pAb in different biological settings. Firstly, levels of serum and peritoneal pAb were measured during early experimental secondary CE, using both high (Balb/c) and low (C57Bl/6) susceptible mouse strains. Serum pAb mostly differed in normal mice, being pAb levels of IgG subclasses with poor anti-parasite activities predominant in Balb/c animals.<br />Conversely, peritoneal pAb isotypes/subclasses with efficient anti-parasite activities predominated in normal and infected C57Bl/6 mice. Secondly, sera from potentially resistant patients, susceptible individuals and healthy donors were analysed, showing higher pAb levels of the IgA and IgG—particularly IgG1—isotypes in potentially resistant individuals compared to control groups. Finally, since remarkable differences were observed in pAb profiles according to the intrinsic host susceptibility to the infection, we proposed here that pAb might be considered as potential humoral biomarkers<br />for host resistance to CE.</p>
Creator
An entity primarily responsible for making the resource
<strong>García Luna, Joaquín</strong>
<strong>Magnone, Javier</strong>
<strong>Miles, Sebastián</strong>
<strong>López Santurio, Camila</strong>
<strong>Dematteis, Sylvia</strong>
<strong>Mourglia Ettlin, Gustavo</strong>
Source
A related resource from which the described resource is derived
Parasite Immunology v. 43, n°3, 2021. -- pp. 1-6. -- e12802
Publisher
An entity responsible for making the resource available
Wiley
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
Rights
Information about rights held in and over the resource
<p dir="ltr"><strong>Información sobre Derechos de Autor</strong></p>
<p dir="ltr"><span>(Por favor lea este aviso antes de abrir los documentos u objetos)</span></p>
<p dir="ltr"><strong>La legislación uruguaya protege el derechode autor</strong><span> sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</span></p>
<span id="docs-internal-guid-9d2103f7-7fff-c813-00c7-2528fd4891ff"><strong>ADVERTENCIA:</strong><span> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</span></span>
Format
The file format, physical medium, or dimensions of the resource
PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
<span id="docs-internal-guid-f6781b37-7fff-23b6-f29f-7d2d19c9cc81"><span>10.1111/pim.12802 </span></span>
Anticuerpos
Biomarcadores
Echinoccocus granulosus
Equinococosis
Hidatosis
-
https://riquim.fq.edu.uy/files/original/08ec376940f5222393a6ee501f4a45d1.pdf
0ebebc2e46cd599f97051dd7aefd7031
https://riquim.fq.edu.uy/files/original/db4671c4ffbe67f573a6213d4d5bb0ce.gif
60e61787f9c4b30b585c8cf8934b4075
Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
150
Width
113
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
Pdf
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Linking murine resistance to secondary cystic echinococcosis with antibody responses targeting Echinococcus granulosus tegumental antigens</strong>
Subject
The topic of the resource
ECHINOCOCCUS GRANULOSUS
ANTICUERPOS
INMUNOINFORMATICA
BIBLIOGRAFIA NACIONAL QUIMICA
2020
Abstract
A summary of the resource.
Successful establishment of a parasite infection depends partially on the host intrinsic susceptibility to the pathogen. In cystic echinococcosis (CE), a zoonotic disease caused by the cestode parasite Echinococcus granulosus, the infection outcome in the murine model of secondary CE varies according to the mouse strain used. In this regard, intrinsic differences in susceptibility to the infection were previously reported for Balb/c and C57Bl/6 mice, being C57Bl/6 animals less permissive to secondary CE. Induction of parasite-specific antibodies has been suggested to play relevant roles in such susceptibility/resistance phenomena. Here, we report an in deep comparison of antibody responses induced in both mouse strains. Firstly, only C57Bl/6 mice were shown to induce specific-antibodies with efficient anti-parasite activities during early secondary CE. Then, through ImmunoTEM and Serological Proteome Analysis (SERPA), an evaluation of specific antibody responses targeting parasite tegumental antigens was performed. Both strategies showed that infected C57Bl/6 mice -unlike Balb/c animals- narrowed their IgG recognition repertoire against tegumental antigens, targeting fewer but potentially more relevant parasite components. In this sense, tegumental antigens recognition between Balb/c and C57Bl/6 mice, either by natural and/or induced antibodies, was analyzed through SERPA and MALDI-TOF/TOF studies. A total of 13 differentially recognized proteins (DRPs) uniquely targeted by antibodies from C57Bl/6 mice were successfully identified, wherein a subset of 7 DRPs were only recognized by infection-induced antibodies, suggesting their potential as natural protective antigens. In this regard, immunoinformatic analyses showed that such DRPs exhibited higher numbers of possible T cell epitopes towards the H-2-IAb haplotype, which is present in C57Bl/6 mice but absent in Balb/c animals. In summary, our results showed that the genetic predisposition to generate better T-dependent antibody responses against particular tegumental antigens might be a key factor influencing host susceptibility in the murine model of secondary CE.
Creator
An entity primarily responsible for making the resource
<strong>Miles, Sebastián</strong>
<strong>Magnone, Javier</strong>
<strong>Cyrklaff, Marek</strong>
<strong>Arbildi, Paula</strong>
<strong>Frischknecht, Friedrich</strong>
<strong>Dematteis, Sylvia</strong>
<strong>Mourglia Ettlin, Gustavo</strong>
Source
A related resource from which the described resource is derived
Inmunobiology 2020
Publisher
An entity responsible for making the resource available
Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
Rights
Information about rights held in and over the resource
<strong>Información sobre Derechos de Autor</strong> (Por favor lea este aviso antes de abrir los documentos u objetos)<strong> La legislación uruguaya protege el derecho de autor sobre toda creación</strong> literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006) ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.
Format
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Pdf
Language
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Inglés
Type
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Artículo
Identifier
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DOI: 10.1016/j.imbio.2020.151916
Anticuerpos
Echinoccocus granulosus
Inmunoinformatica
-
https://riquim.fq.edu.uy/files/original/9a9dae467392aed5f4cf237868c6e262.pdf
eed6e78ea3e17907179a95394ca82563
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
Pdf
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells</strong>
Subject
The topic of the resource
GENES
CELULAS DE OVARIO
ANTICUERPOS
HAMSTER CHINO
BIBLIOGRAFIA NACIONAL QUIMICA
2019
Abstract
A summary of the resource.
Background: The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins in mammalian cell lines. However, these promoters are susceptible to silencing, thus limiting protein productivity. Some CpG islands can avoid the silencing of housekeeping genes; for that reason, they have been used to increase the production of recombinant genes in cells of animal origin. In this study, we evaluated the CpG island of the promoter region of the β-actin gene of Cricetulus griseous (Chinese hamster), associated to the Cytomegalovirus (CMV) promoter, to increase recombinant antibodies production in Chinese Hamster Ovary (CHO) cells. Results: We focused on the non-coding region of CpG island, which we called RegCG. RegCG behaved as a promoter, whose transcriptional activity was mainly commanded by the CAAT and CArG boxes of the proximal promoter. However, the transcription started mainly at the intronic region before the proximal transcription start site. While the CMV promoter was initially more powerful than RegCG, the latter promoter was more resistant to silencing than the CMV promoter in stable cell lines, and its activity was improved when combined with the CMV promoter. Thereby, the chimeric promoter was able to maintain the expression of recombinant antibodies in stable clones for 40 days at an average level 4 times higher than the CMV promoter. Finally, the chimeric promoter showed compatibility with a genetic amplification system by induction with methotrexate in cells deficient in the dihydrofolate reductase gene. Conclusions: We have generated an efficient synthetic hybrid transcription promoter through the combination of RegCG with CMV, which, in stable cell lines, shows greater activity than when both promoters are used separately. Our chimeric promoter is compatible with a genetic amplification system in CHO DG44 cells and makes possible the generation of stable cell lines with high production of recombinant antibodies. We propose that this promoter can be a good alternative for the generation of clones expressing high amount of recombinant proteins, essential for industrial applications.
Creator
An entity primarily responsible for making the resource
<strong>Zuñiga, Roberto A.</strong>
<strong>Gutiérrez González, Matías</strong>
<strong>Collazo, Norberto</strong>
<strong>Sotelo, Pablo Hernán</strong>
<strong>Ribeiro, Carolina H.</strong>
<strong>Altamirano, Claudia</strong>
<strong>Lorenzo, Carmen</strong>
<strong>Aguillón, Juan Carlos</strong>
<strong>Molina, Carmen</strong>
Source
A related resource from which the described resource is derived
Journal of Biological Engineering v. 13, no. 1, 2019. --p. 1-13.--e59
Publisher
An entity responsible for making the resource available
BioMed Central Ltd
Date
A point or period of time associated with an event in the lifecycle of the resource
2019
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong> La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong> ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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Pdf
Language
A language of the resource
Inglés
Type
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Artículo
Identifier
An unambiguous reference to the resource within a given context
DOI: 10.1186/s13036-019-0187-y
Anticuerpos
Celulas de Ovarios
Genes
Hamster Chino
-
https://riquim.fq.edu.uy/files/original/31c3a9906ff7c80fa134755e9b9917a6.pdf
0ebebc2e46cd599f97051dd7aefd7031
https://riquim.fq.edu.uy/files/original/d2bc869b93c30b7cbb8112cd7c557b7d.jpg
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
pdf
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Structure and specificity of several triclocarban-binding single domain camelid antibody fragments</strong>
Subject
The topic of the resource
AGENTES BACTERIOSTATICOS
ANTICUERPOS
TRICLOCARBAN
BIBLIOGRAFIA NACIONAL QUIMICA
2018
Abstract
A summary of the resource.
The variable VHH domains of camelid single chain antibodies have been useful in numerous biotechnology applications due to their simplicity, biophysical properties, and abilities to bind to their cognate antigens with high affinities and specificity. Their interactions with proteins have been well‐studied, but considerably less work has been done to characterize their ability to bind haptens. A high‐resolution structural study of three nanobodies (T4, T9, and T10) which have been shown to bind triclocarban (TCC, 3‐(4‐chlorophenyl)‐1‐(3,4‐dichlorophenyl)urea) with near‐nanomolar affinity shows that binding occurs in a tunnel largely formed by CDR1 rather than a surface or lateral binding mode seen in other nanobody‐hapten interactions. Additional significant interactions are formed with a non‐hypervariable loop, sometimes dubbed “CDR4”. A comparison of apo and holo forms of T9 and T10 shows that the binding site undergoes little conformational change upon binding of TCC. Structures of three nanobody‐TCC complexes demonstrated there was not a standard binding mode. T4 and T9 have a high degree of sequence identity and bind the hapten in a nearly identical manner, while the more divergent T10 binds TCC in a slightly displaced orientation with the urea moiety rotated approximately 180° along the long axis of the molecule. In addition to methotrexate, this is the second report of haptens binding in a tunnel formed by CDR1, suggesting that compounds with similar hydrophobicity and shape could be recognized by nanobodies in analogous fashion. Structure‐guided mutations failed to improve binding affinity for T4 and T9 underscoring the high degree of natural optimization.
Creator
An entity primarily responsible for making the resource
<strong>Tabares da Rosa, Sofía</strong>
<strong>Wogulis, Linda A.</strong>
<strong>Wogulis, Mark D.</strong>
<a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20&formato=pdf&convocatoria=21" target="_self"><strong>González Sapienza, Gualberto.</strong></a>
<strong>Wilson, David K.</strong>
Source
A related resource from which the described resource is derived
Journal of Molecular Recognition 2018.--p. 1-9.--e2755
Publisher
An entity responsible for making the resource available
Wiley
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
DOI: 10.1002/jmr.2755
Agentes Bacterioestaticos
Anticuerpos
Triclocarban
-
https://riquim.fq.edu.uy/files/original/0c4c7c0944b62f249f54eeb257f25cd0.pdf
0ebebc2e46cd599f97051dd7aefd7031
https://riquim.fq.edu.uy/files/original/60b0f5a11123aa42e2444e5ec57a0b1d.jpg
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Ageing impairs parasite-specific antibody responses in cystic echinococcosis</strong>
Subject
The topic of the resource
PARASITOLOGIA
QUISTE HIDATICO
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
2018
Abstract
A summary of the resource.
Humoral immunity wanes during healthy ageing, increasing susceptibility to infections in the elderly. In this sense, information about parasite infections and human immunosenescence is scarce. Cystic echinococcosis (CE) is an infectious disease caused by the larval stage of the cestode parasite Echinococcus granulosus, whose prevalence in humans shows an increase with host age. Susceptibility to human CE has been associated with humoral immunity to some extent; and therefore, we have here analyzed the influence of host age on the serological profile of young, middle-aged, and aged patients. Our results highlighted the detrimental influence of ageing on the intensity and quality of the anti-parasite antibody response. Remarkable differences in serological profiles between young and aged individuals were observed. In this sense, through Principal Components Analysis we identified aged patients as those exhibiting overall less intense antibody responses, mainly in isotypes/subclasses supposed to exert efficient anti-parasite activities (e.g. IgE and IgG1). Thus, these humoral defects could at least partially explain the reported increase in CE prevalence among older individuals, since a weaker immune response in the elderly might facilitate the establishment and maintenance of the parasite infection. Finally, a possible association between age-dependent susceptibility to CE and host immunosenescence is discussed.
Creator
An entity primarily responsible for making the resource
<strong>Mourglia Ettlin, Gustavo Daniel.</strong>
<strong>Miles, S.</strong>
<strong>Hernández, A.</strong>
<a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=086432105a0be10ef4e4e57df7e51e5a096203688a890b013ec9a12794f41c17f3cd780253064d2d2485395b80b566f9216f3b1d82011d108cd4ea1ac4e03220&formato=pdf&convocatoria=21" target="_self"><strong>Dematteis, Sylvia.</strong></a>
Source
A related resource from which the described resource is derived
Parasite Immunology v. 40, no. 1, 2018. --4p.--e12505
Publisher
An entity responsible for making the resource available
Wiley
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
The file format, physical medium, or dimensions of the resource
PDF
Language
A language of the resource
inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
DOI: 10.1111/pim.12505
Anticuerpos
Parasitología
Quiste hidático
-
https://riquim.fq.edu.uy/files/original/b7a85be21fd4107eff16444b9e49c477.jpg
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
84
Width
146
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
Papel
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Composición química de PMSG (Pregnant Mare Serum Gonadotropin) : inmunología de PMSG</strong>
Subject
The topic of the resource
<strong>PMSG</strong>
<strong>ECG</strong>
<strong>GONADOTROPINAS</strong>
<strong>CABALLOS</strong>
<strong>COMPOSICIÓN QUIMICA</strong>
<strong>AMINOACIDOS</strong>
<strong>INMUNOLOGIA</strong>
<strong>ANTICUERPOS</strong>
Publisher
An entity responsible for making the resource available
Biblioteca-FQ
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
Format
The file format, physical medium, or dimensions of the resource
Papel
PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Bibliografía
Temporal Coverage
Temporal characteristics of the resource.
1984-1991
Aminoácidos
Anticuerpos
Caballos
Composición química
ECG
Gonadotropinas
Inmunología
PMSG
-
https://riquim.fq.edu.uy/files/original/684d887b69ab6425b46ec761e08e0032.pdf
f4be5d6a55128546ea446f4cac4f93a1
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
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3
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175
Width
134
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Linear and extended : a common polyglutamine conformation recognized by the three antibodies MW1, 1C2 and 3B5H10</strong>
Subject
The topic of the resource
PEPTIDOS
GLUTAMINA
POLIGLUTAMINA
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
2013
Abstract
A summary of the resource.
A long-standing pathomechanistic model proposes that the polyglutamine (polyQ)-length-dependent toxicity threshold observed in all polyQ diseases is triggered by a conformational change within the monomer that occurs only above a certain polyQ length. If true, this yet undefined and elusive mutant-specific toxic conformation would constitute a direct therapeutic target. Three anti-polyQ antibodies—MW1, 1C2 and 3B5H10—have been extensively used to probe the conformation of polyQ. The crystal structure of the MW1 epitope reveals a linear, non-pathogenic polyQ. In contrast, although the detailed structure of its epitope is unknown, the 3B5H10 antibody is widely advertised and used as a conformational antibody that recognizes the toxic conformation of expanded polyQ. We solved the crystal structure of the 1C2 antigen-binding domain (1C2-Fab) and performed a direct comparison between the 1C2, MW1 and 3B5H10 structures. The MW1 and 1C2 antibodies have similar sequences and structures, consistent with their binding to short polyQ and their polyQ length-discrimination properties. Unexpectedly, the 3B5H10 antibody also shares striking features with MW1 and 1C2, which prompted us to revisit its binding properties. We show that the 3B5H10 epitope is actually a short, non-pathogenic polyQ. All three antibodies MW1, 1C2 and 3B5H10 interact similarly with polyQ of various lengths, and bind small polyQ epitopes in similar linear and extended conformations. Together with studies published during the recent years, our work argues against the hypothesis that a mutant-specific conformation in monomeric polyQ molecules is the toxic entity responsible for polyQ diseases.<br /><br />
Creator
An entity primarily responsible for making the resource
<strong>Klein Fabrice, A. C.</strong>
<strong>Zeder Lutz, Gabrielle</strong>
<strong>Cousido Siah, Alexandra</strong>
<strong>Mitschler, Andre</strong>
<strong>Katz, Aline</strong>
<strong>Eberling, Pascal</strong>
<strong>Mandel, Jean Louis</strong>
<strong>Podjarny, Alberto</strong>
<strong>Trottier, Yvon</strong>
Source
A related resource from which the described resource is derived
Human Molecular Genetics v. 22, no. 20, 2013. -- p. 4215-4223
Publisher
An entity responsible for making the resource available
Oxford Academic
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
Rights
Information about rights held in and over the resource
<p style="margin-bottom: 0cm;"><strong>Información sobre Derechos de Autor</strong><br /><br />(Por favor lea este aviso antes de abrir los documentos u objetos)<br /><br /><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes<br /><br />(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)<br /><br /><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
The file format, physical medium, or dimensions of the resource
PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
https://doi.org/10.1093/hmg/ddt273
Anticuerpos
Glutamina
Péptidos
Poliglutamina
-
https://riquim.fq.edu.uy/files/original/de67d34190bb97e5d9a59f95e89310b2.pdf
0ebebc2e46cd599f97051dd7aefd7031
https://riquim.fq.edu.uy/files/original/56cbfbbd47f5e6d9b19eb39d642bffc8.jpg
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
208
Width
150
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Obtención de anticuerpos monoclonales frente al antígeno hidatídico y su caracterización</strong>
Subject
The topic of the resource
ANTICUERPOS
ANTICUERPOS MONOCLONALES
HIDATIDOSIS
BIBLIOGRAFIA NACIONAL QUIMICA
1987
Creator
An entity primarily responsible for making the resource
<strong>Muñoz, C.</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8" target="_blank"><strong>Nieto, A.</strong></a>
<strong>Gayá, A.</strong>
<strong>Mazie, J.</strong>
Source
A related resource from which the described resource is derived
Revista Ibérica de Parasitología. Sup., 1987. -- p. 229-237
Publisher
An entity responsible for making the resource available
<span class="valor">Instituto José de Acosta. Secciones de Hemintología y Parasitología. Universidad de Granad</span>a
Date
A point or period of time associated with an event in the lifecycle of the resource
1987
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong> La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
ISSN: 0034-9623.
Anticuerpos
Anticuerpos monoclonales
Hidatidosis
-
https://riquim.fq.edu.uy/files/original/4675c6338fb2986a1788c859c973b604.pdf
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https://riquim.fq.edu.uy/files/original/a074882e86cbf8182c9432059c8a7ac8.jpg
766ede8a79c33f24b93cb484556e19ba
Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
198
Width
157
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<p><strong>Título y avidez de anticuerpos antigliadina en enfermos celíacos (Ec)</strong></p>
Subject
The topic of the resource
ANTICUERPOS
ENFERMEDAD CELÍACA
ANTICUERPOS ANTIGLIADINA
ENFERMEDADES AUTOINMUNES
BIBLIOGRAFIA NACIONAL QUIMICA
1989
Abstract
A summary of the resource.
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" alt="" />
Creator
An entity primarily responsible for making the resource
<strong>Molina, M.C.</strong>
<a title="Curriculum vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8b2cacb0aafc512133624fa7b9bf174c" target="_blank"><strong>Salinas, G.</strong></a>
<a title="Curriculum vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8" target="_blank"><strong>Nieto, Alberto</strong></a>
Source
A related resource from which the described resource is derived
Inmunología v. 8, no. 3, 1989. -- p. 102-106
Rights
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<p class="western" lang="es-ES" align="left"><strong>Información sobre Derechos de Autor</strong></p>
<p class="western" lang="es-ES">(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p class="western" lang="es-ES"><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p class="western" lang="es-ES">(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p class="western" style="margin-bottom: 0cm;" lang="es-ES"><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Español
Type
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Artículo
Identifier
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ISSN (digital) 0213-0626
1989
Anticuerpos
Anticuerpos antigliadina
Bibliografía Nacional Química
Enfermedad celíaca
Enfermedades autoinmunes
-
https://riquim.fq.edu.uy/files/original/150bc7992b1f614cb7517588ad1b1247.bmp
c7a359daa12e23281c4edb5469c50d2d
https://riquim.fq.edu.uy/files/original/4e21d660006632429b1f53980cc52bbb.pdf
e178036d9674f251826713e8851bbff2
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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<strong>Natural and induced antibodies contribute to differentialsusceptibility to secondary cystic echinococcosis of Balb/c andC57Bl/6 mice</strong>
Subject
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ECHINOCOCCUS GRANULOSUS
INMUNOBIOLOGIA
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
2016
Abstract
A summary of the resource.
tAntibodies are key immune players in several helminth infections and animal models have been centralfor the identification of their mechanisms of protection. Murine secondary cystic echinococcosis is auseful model for studying Echinococcus granulosus immunobiology, being the immune profile mountedby the experimental host a determinant of parasite success or failure in infection establishment. In thepresent study, we analyzed infection outcome using Balb/c and C57Bl/6 mice strains, and compared theirantibody responses in terms of quality and intensity. Our results showed that Balb/c is a highly susceptiblestrain to secondary cystic echinococcosis, while C57Bl/6 mice are quite resistant. Moreover, significantdifferences between strains were observed in natural and induced antibodies recognizing E. granulosusantigens, both at the systemic and peritoneal levels. Natural cross-reacting IgM, IgG2b and IgG3 antibodieswere detected in sera from both strains but with different intensities, and – remarkably – natural IgG2bshowed to be an intrinsic correlate of protection in both mice strains. Interestingly, naïve C57Bl/6 serumdisplayed a higher protoscolicidal activity, and heterologous – but not homologous – transference ofC57Bl/6 naïve serum into Balb/c mice, significantly reduced their infection susceptibility. In the peritonealcavity, different levels of natural cross-reacting IgM and IgG3 antibodies were detected in both micestrains, while cross-reacting IgG2b was detected only in C57Bl/6 mice. On the other hand, infected micefrom both strains developed isotype-mixed antibody responses, with Balb/c mice biasing their responsetowards high avidity IgG1 and C57Bl/6 mice showing a predominance of mixed IgM/IgG2c/IgG2b/IgG3.In this regard, IgG1 levels showed to be a correlate of susceptibility in both mice strains. In conclusion,our results suggest that antibodies – either natural or induced – play a role in the susceptibility degreeto murine secondary cystic echinococcosis.
Creator
An entity primarily responsible for making the resource
<strong>Mourglia Ettlin, Gustavo Daniel</strong>
<strong>Cucher, Marcela</strong>
<strong>Arbildi, Paula</strong>
<strong>Rosenzvit, Mara</strong>
<a title="Currículum vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=3d2eba59d2d4c6ef6f52fa30f8fc1854" target="_blank"><strong>Dematteis, Sylvia</strong></a>
Source
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Immunobiology v. 221, 2016. -- p. 103-115
Publisher
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Elsevier
Date
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2016
Rights
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<p align="LEFT"><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p style="margin-bottom: 0cm;"><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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http://dx.doi.org/10.1016/j.imbio.2015.07.016
Anticuerpos
Echinococcus Granulosus
Inmunobiología
-
https://riquim.fq.edu.uy/files/original/9a18eb4d506299db861c2123a15f5800.pdf
887368cdcfd41924a3752d0133b638f1
https://riquim.fq.edu.uy/files/original/a08af00fc2ba0de8d6ffce999d10fa06.jpg
7fbb1866d05413c200fff65a99c088b5
Omeka Image File
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Bit Depth
8
Channels
3
Height
182
Width
128
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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<strong>A Model to explain the influence of maternal idiotypes or antiidiotypes on the selection of available repertoires</strong>
Subject
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IDIOTIPO
ANTI-IDIOTIPO
ANTICUERPOS
1991
BIBLIOGRAFIA NACIONAL QUIMICA
Creator
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<strong>Dewit, D.</strong>
<strong>Baz, A.</strong>
<strong>Demeur, C.</strong>
<strong>Ryelandt, M.</strong>
<strong>Urbain, J.</strong>
<strong>Vansanten, G.</strong>
Source
A related resource from which the described resource is derived
Cellular Molecular Biology of the Maternal-Fetal Relationship v. 212, , 1991. -- p. 151-157
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong> La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong> ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Capítulo de libro
Identifier
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ISSN 0768-3154
Anti-Ideotipo
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Ideotipo
-
https://riquim.fq.edu.uy/files/original/791ef36838657d8048ecd71680d4ea17.pdf
47a04f8f934ca1a865868e503e4e507f
https://riquim.fq.edu.uy/files/original/4c7c803a83eebc7fa53f16d55ede502a.jpg
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3
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Title
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Bibliografía Nacional Química
Description
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Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
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Departamento de Documentación y Biblioteca
RIQUIM
Document
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PDF
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Title
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<strong>Streamlined method for parallel identification of single domain antibodies to membrane receptors on whole cells</strong>
Subject
The topic of the resource
ANTICUERPOS
RECEPTOR DE LA CELULA
BIBLIOGRAFIA NACIONAL QUIMICA
2015
Abstract
A summary of the resource.
Background<br /><br />Owing to their minimal size, high production yield, versatility and robustness, the recombinant variable domains (nanobodies) of camelid single chain antibodies are valued affinity reagents for research, diagnostic, and therapeutic applications. While their preparation against purified antigens is straightforward, the generation of nanobodies to difficult targets such as multi-pass or complex membrane cell receptors remains challenging. Here we devised a platform for high throughput identification of nanobodies to cell receptor based on the use of a biotin handle.<br />Methods<br /><br />Using a biotin-acceptor peptide tag, the in vivo biotinylation of nanobodies in 96 well culture blocks was optimized allowing their parallel analysis by flow cytometry and ELISA, and their direct use for pull-down/MS target identification.<br />Results<br /><br />The potential of this strategy was demonstrated by the selection and characterization of panels of nanobodies to Mac-1 (CD11b/CD18), MHC II and the mouse Ly-5 leukocyte common antigen (CD45) receptors, from a VHH library obtained from a llama immunized with mouse bone marrow derived dendritic cells. By on and off switching of the addition of biotin, the method also allowed the epitope binning of the selected Nbs directly on cells.<br />Conclusions<br /><br />This strategy streamlines the selection of potent nanobodies to complex antigens, and the selected nanobodies constitute ready-to-use biotinylated reagents.<br />General significance<br /><br />This method will accelerate the discovery of nanobodies to cell membrane receptors which comprise the largest group of drug and analytical targets.<br /><br />
Creator
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<strong>Rossotti, Martín</strong>
<strong>Tabares, Sofía</strong>
<strong>Alfaya, Lucía</strong>
<strong>Leizagoyen, Carmen</strong>
<strong>Moron, Gabriel</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=7ee0def1187be47e0710e877d9768b5a" target="_blank"><strong>González-Sapienza, Gualberto G.</strong></a>
Source
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Biochimica et Biophysica Acta v. 1850, no. 7, 2015. -- p. 1397-1404
Publisher
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Elsevier
Date
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2015
Rights
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<p><strong>Información sobre Derechos de Autor</strong><br /> <br /> (Por favor lea este aviso antes de abrir los documentos u objetos)<br /> <br /> <strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes<br /> <br /> (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)<br /> <br /> <strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
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PDF
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Inglés
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Artículo
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doi:10.1016/j.bbagen.2015.03.009
Anticuerpos
Receptor de la celula
-
https://riquim.fq.edu.uy/files/original/044c234e50a13b3474f8d3fa15274606.pdf
47a04f8f934ca1a865868e503e4e507f
https://riquim.fq.edu.uy/files/original/c069120cef2da2a26358dd457c6df45e.jpg
e3fab2d8a6ab8db4d4b28e63bf7e3f7e
Omeka Image File
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
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PDF
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Title
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<strong>Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays</strong>
Subject
The topic of the resource
INMUNOLOGIA
CLONACION
ANTICUERPOS
HIDROLASAS
BIBLIOGRAFIA NACIONAL QUIMICA
2015
Abstract
A summary of the resource.
Single domain heavychain binders (nanobodies) obtained from camelid antibody libraries hold a great promise for immunoassay development. However, there is no simple method to select the most valuable nanobodies from the crowd of positive clones obtained after the initial screening. In this paper, we describe a novel nanobody-based platform that allows comparison of the reactivity of hundreds of clones with the labeled antigen, and identifies the best nanobody pairs for two-site immunoassay development. The output clones are biotinylated in vivo in 96-well culture blocks and then used to saturate the biotin binding capacity of avidin coated wells. This standardizes the amount of captured antibody allowing their sorting by ranking their reactivity with the labeled antigen. Using human soluble epoxide hydrolase (sEH) as a model antigen, we were able to classify 96 clones in four families and confirm this classification by sequencing. This provided a criterion to select a restricted panel of five capturing antibodies and to test each of them against the rest of the 96 clones. The method constitutes a powerful tool for epitope binning, and in our case allowed development of a sandwich ELISA for sEH with a detection limit of 63 pg/mL and four log dynamic range, which performed with excellent recovery in different tissue extracts. This strategy provides a systematic way to test nanobody pairwise combinations and would have a broad utility for the development of highly sensitive sandwich immunoassays.
Creator
An entity primarily responsible for making the resource
<strong>Rossotti, Martín</strong>
<strong>Pirez, Macarena</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=67c04f6a200d5de027ddb6804d7bae5f" target="_blank"><strong>González Techera, Andrés</strong></a>
<strong>Cui, Yongliang</strong>
<strong>Bever, C. S.</strong>
<strong>Morisseau, C.</strong>
<strong>Leizagoyen, Carmen</strong>
<strong>Gee, Shirley</strong>
<strong>Hammock, B. D</strong><br /><br />
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=7ee0def1187be47e0710e877d9768b5a" target="_blank"><strong>González-Sapienza, Gualberto G.</strong></a>
Source
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Analytical Chemistry v. 87, no. 23, 2015. -- p. 11907-14
Publisher
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American Chemical Society
Date
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2015
Rights
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<p><strong>Información sobre Derechos de Autor</strong><br /> <br /> (Por favor lea este aviso antes de abrir los documentos u objetos)<br /> <br /> <strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes<br /> <br /> (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)<br /> <br /> <strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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DOI: 10.1021/acs.analchem.5b03561
Anticuerpos
Clonación
Hidrolasas
Inmunología
-
https://riquim.fq.edu.uy/files/original/015eb9a40dedf4b5f8b4cb8a8352181e.pdf
e178036d9674f251826713e8851bbff2
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
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8
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
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PDF
Dublin Core
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Title
A name given to the resource
<strong> The perinatal presence of antigen (p-azophenylarsonate) or anti-u antibodies lead to the loss of the recurrent idiotype (CRIA) in A/J mice.</strong>
Subject
The topic of the resource
ANTIGENOS
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
1994
Abstract
A summary of the resource.
The immune response of A/J mice against p-azophenylarsonate (Ars)—keyhole limpet hemocyanln(KLH) is characterized by the dominance, late in primary and during the secondary, of a recurrent Idiotype called CRIA, encoded by a canonical combination of Ig gene segments. In this study, A/J mice were given Ars coupled to deaggregated human gamma globulins (dHGG) within 24 h after delivery. The offsprings from these mice were then exposed as adults to Ars—KLH. These animals developed an unusual immune response. The level of anti-Ars antibodies was nearly normal but a dramatic shift In repertoire was observed: the cross-reactive idiotype which is the hallmark of the anti-Ars response in A/J mice was completely absent. The idiotype could be recovered by injection of anti-idiotypic antibodies alone, with no need of llpopolysaccharlde coupling. Therefore the presence of antigen at birth can lead to a strong perturbation of idiotype selection. Similar results were obtained with neonatal treatment using antl-IgM antibodies. After recovery of suppression, A/J mice can mount an anti-arsonate response of normal level but devoid of the dominant idiotype.
Creator
An entity primarily responsible for making the resource
<strong>Ryelandt, M</strong>
<strong>De Wit, D</strong>
<strong>Baz, A</strong>
<strong>Huetz, F</strong>
<strong>Nisol, F</strong>
<strong>Macedo, F</strong>
<strong>Barcy, S</strong>
<strong> Brait, M</strong>
<strong>Leo, O</strong>
<strong>Bazin, H</strong>
<strong>Urbain, J</strong>
Source
A related resource from which the described resource is derived
International Immunology v. 4, 1994. -- p. 645-652
Publisher
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The Japanese Society for Immunology
Date
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1994
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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doi: 10.1093/intimm/7.4.645
Anticuerpos
Antígenos
-
https://riquim.fq.edu.uy/files/original/9982c92eae24f72e21f9c414f726cc4a.pdf
e178036d9674f251826713e8851bbff2
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Use of specific antibody and circulating antigen serum levels in the hydatid immunodiagnosis of asymptomatic population.</strong>
Subject
The topic of the resource
ANTICUERPOS
ANTIGENO
QUISTE HIDATICO
INMUNODIAGNOSTICO
HIDATIDOSIS
BIBLIOGRAFIA NACIONAL QUIMICA
1994
Abstract
A summary of the resource.
Levels of specific antibodies (Ab) and circulating antigens (CAg) were tested by ELISA in sera from 115 surgically confirmed hydatid patients, 41 individuals exhibiting other parasitic and unrelated diseases and 69 healthy subjects. Addition of CAg data to Ab detection in this sera collection increased sensitivity from 85% (only Ab) to 89% (Ab + CAg). Combination of ultrasonography with Ab and CAg serology for diagnosis of asymptomatic population in endemic areas was analyzed. One field survey (163 persons) involved both blood extraction and ultrasonography to all the population. Three people exhibited cyst images and all of them were Ab positive, while 6 Ab and 1 CAg positive individuals exhibited no cyst image. Another survey (1620 persons) involved a selection of 85 subjects for serology according to ultrasound data and record of family hydatid history. Twelve per cent exhibited no hydatid image being serologically positive and 14% were serologically negative but exhibited cyst image. Ultrasonography and serology (Ab and CAg) should be used in combination to maximize the diagnostic yield in asymptomatic population.
Creator
An entity primarily responsible for making the resource
<strong>Barbieri, Mariela</strong>
<strong>Severi, María Angélica</strong>
<strong>Pírez, Maria Ines</strong>
<strong>Battistoni, Julio</strong>
<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8"><strong>Nieto, Alberto</strong></a>
Source
A related resource from which the described resource is derived
International Journal for Parasitology v. 24, no. 7, 1994. -- p. 937-942
Publisher
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Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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doi:10.1016/0020-7519(94)90157-0
Anticuerpos
Antígenos
Hidatidosis
Inmunodiagnóstico
Quiste hidático
-
https://riquim.fq.edu.uy/files/original/ee8a547e3a8b2038a09b7a50dc0a69a1.pdf
e178036d9674f251826713e8851bbff2
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Omeka Image File
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101
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong> Human isotype antibody responses to an Onchocerca volvulus glutathione S-transferase.</strong>
Subject
The topic of the resource
AGLUTINACION
ANTICUERPOS
ISOTIPOS
ONCHOCERCA VOLVULUS
GLUTATIONA S-TRANFERASA
BIBLIOGRAFIA NACIONAL QUIMICA
1996
Creator
An entity primarily responsible for making the resource
<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8b2cacb0aafc512133624fa7b9bf174c"><strong>Salinas, Gustavo</strong></a>
<strong>Sinha, K</strong>
<strong>Cooper, J. P</strong>
<strong>Whitworth, J</strong>
<strong>Taylor, D</strong>
Source
A related resource from which the described resource is derived
Parasite Immunology v. 18, no. 8, 1996. -- p. 377-386
Publisher
An entity responsible for making the resource available
Wiley
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
An unambiguous reference to the resource within a given context
DOI: 10.1046/j.1365-3024.1996.d01-124.x
Aglutinación
Anticuerpos
Glutationa s-tranferasa
Isotipos
Onchocerca volvulus
-
https://riquim.fq.edu.uy/files/original/813f8466f2f9e87300f862b83798f18d.pdf
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Bit Depth
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131
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
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Departamento de Documentación y Biblioteca
RIQUIM
Document
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If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
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Title
A name given to the resource
<strong>Antibody response of Echinococcus granulosus infected mice : recognition of glucidic and peptidic epitopes and lack of avidity maturation.</strong>
Subject
The topic of the resource
ECHINOCOCCUS GRANULOSUS
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
1996
Abstract
A summary of the resource.
. granulosus;secondary hydatidosis in mice;carbohydrate epitopes;antibody avidity The antibody response was followed weekly during 68 weeks in 17 Balb/c mice intraperitoneally (i.p.) infected with 2000 Echinococcus granulosus protoscoleces (PSC) and in three mice i.p. immunized with 2000 dead PSC. Antibodies against hydatid cyst fluid (HCFA) and its peptidic (periodate-resistant) and carbohydrate (periodate-sensitive) epitopes were titrated by ELISA. Avidity and the antigen recognition pattern of antibodies were also analysed during infection and immunization by ELISA and immunoblot, respectively. The antibody response of infected mice showed quantitative and qualitative variations during infection, since both titre as well as recognition of peptide and carbohydrate epitopes in HCFA depended on time post infection. No avidity maturation was evident during the course of infection. Sera from infected mice recognized the 38 kDa subunit of Ag5 but did not react with the 8 kDa subunit of AgB. On the contrary, the antibody response of immunized mice showed only one peak of antibodies that recognized both peptidic and carbohydrate epitopes of HCFA. In addition, sera from these mice recognized mainly 60 and 110 kDa bands. Our results suggest that: a) avidity and antigen recognition patterns of antibodies in mice treated with live PSC are different from those treated with dead PSC; b) antibodies against HCFA glucidic or peptidic epitopes appear at different times post infection.
Creator
An entity primarily responsible for making the resource
<strong>Ferragut, Gabriela</strong>
<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8"><strong>Nieto, Alberto</strong></a>
Source
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Parasite Immunology v. 18, , 1996. -- p. 393-402
Publisher
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Wiley
Date
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1997
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
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PDF
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Inglés
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Artículo
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DOI: 10.1046/j.1365-3024.1996.d01-125.x
Anticuerpos
Echinoccocus granulosus
-
https://riquim.fq.edu.uy/files/original/dbab940074110071be41eb812ece67d0.pdf
e178036d9674f251826713e8851bbff2
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8
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3
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
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Title
A name given to the resource
<strong>Chloroactivated latex particles for covalent coupling of antibodies. Application to immunoassays.</strong>
Subject
The topic of the resource
LATEX
ANTICUERPOS
INMUNOENSAYOS
BIBLIOGRAFIA NACIONAL QUIMICA
1997
Abstract
A summary of the resource.
The aim of the present work is to prepare and characterize a functionalized latex with chloromethyl groups on the surface and to perform the covalent coupling of anti-human serum albumin (a-HSA) IgG protein. The chloromethyl-styrene latex (CMS) was synthesized by means of a core-shell emulsion polymerization in a batch reactor. The monodisperse-obtained latex was characterized by determining the diameter (TEM and PCS), the surface charge density (conductometric and potentiometric titration), the amount of chloromethyl groups on the surface (hydrolysis reaction), and the stability vs electrolyte concentration (turbidity measurements). Electrokinetic characterization was also performed (electrophoretic mobility versus pH and ionic strength). IgG was chemically bound to the latex particles under different sensitization and block-stabilization conditions. Colloidal stability of complexes was studied to select an immunolatex suitable for the development of latex immunoassays. The final part of this work consists of a study of the immunoreactivity of the IgG-latex complexes at different pH and ionic strength, in particular under physiological conditions. The results show that chemically bound IgG to chloromethyl latex provides an IgG-latex complex suitable for application in immunodiagnosis tests.
Creator
An entity primarily responsible for making the resource
<strong>Miraballes, I</strong>
<strong>Martín, A</strong>
<strong> Hidalgo, R</strong>
Source
A related resource from which the described resource is derived
Journal of Biomaterials Science, Polymer edition v. 8, 1997. -- p. 765-777
Publisher
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Taylor and Francis
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
An unambiguous reference to the resource within a given context
DOI: 10.1163/156856297X00308
Anticuerpos
Inmuno ensayo
Látex
-
https://riquim.fq.edu.uy/files/original/a77f6fa37dede63201cf1377d8789148.pdf
39b6a031ade0b3d955e03c24c070c3ca
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
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8
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Analysis of cytokine and specific antibody profile in hydatid patients with primary infection and relapse of diseas.</strong>
Subject
The topic of the resource
CITOCINAS
ANTICUERPOS
HIDATIDOSIS
BIBLIOGRAFIA NACIONAL QUIMICA
1997
Abstract
A summary of the resource.
We studied in vitro cytokine production by peripheral blood mononuclear cells (PBMC) from patients with primary and recurrent hydatid disease when cells were incubated with mitogen (PHA) and antigen from hydatid cyst fluid (HCFAg); levels of specific IgE, IgG4 and eosinophil counts were also measured in sera. When specifically stimulated, PBMC from patients produced higher levels of IL-2 (P < 0·02), IFN- g (P < 0·0028) and IL-5 (P < 0·01) than those from uninfected donors, whereas IL-10 levels were comparable. Notably, IL-5 was also produced in higher levels (P < 0·01) by PBMC from patients when incubated with PHA. The IL-5:IFN- g ratio was significantly greater (P < 0·02) when measured in response to specific stimulation than it was for PHA-stimulated cultures. These cytokine data suggest a bias towards a Th2-response which is in agreement with the high levels of IgG4 and IgE observed. The polarized response appears to be related to clinical status, as differences between patients with primary infec- tion and those with relapse of disease were demonstrated, with significantly higher levels of IgE (P < 0·003), IgG4 (P < 0·04) titres and eosinophil counts (P < 0·04) in the latter; in addition a tendency to an increased production of IL-5 buy lower IFN- g was also observed in this group. These results merit further study as they are suggestive of a putative role of Th2-like responses in susceptibility to reinfection by E. granulosus
Creator
An entity primarily responsible for making the resource
<strong>Hernández, A</strong>
<strong>Borras, R</strong>
<strong> Mir, G</strong>
Source
A related resource from which the described resource is derived
Parasite Immunology, vol 19, 1997. -- p. 553-561
Publisher
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Blackwell
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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DOI: 10.1046/j.1365-3024.1997.d01-173.x
Anticuerpos
Citocinas
Hidatidosis
-
https://riquim.fq.edu.uy/files/original/65cd454c375641f9b86b643614ff7fdd.pdf
e178036d9674f251826713e8851bbff2
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Omeka Image File
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8
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3
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308
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230
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Evolution of IgG antibody response against T.gondii tissue cyst in acute and chronic human infections.</strong>
Subject
The topic of the resource
TRYPANOSOMA GONDII
INMUNOGLOBULINA - IgG
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
1998
Abstract
A summary of the resource.
The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected patients at acute and chronic phase. There was an overall coincidence of molecular mass among antigens of tachyzoites and tissue cysts recognized by these sera, however, they appear not to be the same molecules. The response against tissue cysts starts early during acute infection, and the reactivity of antibodies is strong against a wide range of antigens. Six bands (between 82 and 151 kDa) were exclusively recognized by chronic phase sera but only the 132 kDa band was positive in more than 50% of the sera analysed. A mixture of these antigens could be used to discriminate between the two infection phases. The most important antigens recognized by the acute and the chronic phase sera were 4 clusters in the ranges 20-24 kDa, 34-39 kDa, 58-80 kDa and 105-130 kDa as well as two additional antigens of 18 and 29 kDa. Both accidentally infected patients and some of the naturally infected patients showed a weak specific response against tissue cyst antigens.
Creator
An entity primarily responsible for making the resource
<strong>Villavedra, M</strong>
<strong>Carol, H</strong>
<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8"><strong>Nieto, Alberto</strong></a>
Source
A related resource from which the described resource is derived
Revista do Instituto de Medicina Tropical de Sao Paulo v. 40, no. 2, 1998. -- p. 77-84
Publisher
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Instituto de Medicina Tropical
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora
Format
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PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
ISSN 0036-4665
Anticuerpos
Inmunoglobulina - IgG
Trypanosoma gondii
-
https://riquim.fq.edu.uy/files/original/23ea0d0104683cf4c186536320647943.pdf
2bab74293de97f01c501b9c4db7d1123
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Omeka Image File
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8
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3
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Preparation of reagents from blood group serology : illustrating basic concepts of the antibody respons.</strong>
Subject
The topic of the resource
SEROLOGIA
ANTICUERPOS
URUGUAY
BIBLIOGRAFIA NACIONAL QUIMICA
1998
Abstract
A summary of the resource.
The aim of the present work is to illustrate basic issues relating to the antibody response through the preparation reagents for blood group serology. Two rabbits were immunised with the stromata of A and B cells. The antibody response was analysed by ELISA and Haemagglutination. The appropriate bleeding was selected in order to prepare the reagents. They were tested by blood grouping a panel of volunteers and comparing the results with those obtained with a commercial kit.
Creator
An entity primarily responsible for making the resource
<strong>Miguez, M</strong>
<strong>Marco, M</strong>
<strong>Caceres, S</strong>
<a title="Curriculum" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=9d36a9655c915bddd80b664491d73ec8"><strong>Nieto, Alberto</strong></a>
Source
A related resource from which the described resource is derived
Biochemical Education v. 26, , 1998. -- p. 168-172
Publisher
An entity responsible for making the resource available
Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Uruguay
Anticuerpos
Serología
Uruguay
-
https://riquim.fq.edu.uy/files/original/1313b6cb294f96785589c655aeb8f218.pdf
e178036d9674f251826713e8851bbff2
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Characterization of MARCKS (Myristoylated Alanine-Rich C Kinase Substrate) identified by a monoclonal antibody generated against chick embryo neural retina.</strong>
Subject
The topic of the resource
BIOLOGIA MOLECULAR
BIOLOGIA CELULAR
PROTEINAS
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
1999
Abstract
A summary of the resource.
To identify molecular markers of cell differentiation in developing nervous tissue, monoclonal antibodies against chick embryo neural retina were made. One of them, 3C3mAb, recognized a developmentally regulated antigen present in several organs of the CNS. Data from MALDI-TOF mass spectrometry and peptide sequencing of the immuno-affinity purified protein indicated identity of the antigen with MARCKS. The immunoreactive material was always found as a unique polypeptide (Mr 71 kDa) in SDS-PAGE, however isoelectrofocusing revealed the existence of several bands (pI ranging from 4.0 to 4.5). Interestingly some retinal cell types, as photoreceptors, exhibited an extremely significant decrease in the intensity of the immunoreactive material during the final phases of terminal differentiation while others, as some retinal neurons, maintained the immunoreactivity when fully differentiated. Taken together these results indicate that MARCKS, a protein susceptible of several posttranslational modifications as myristoylation and phosphorylation at variable extent, may act differently in neural retina cell types.
Creator
An entity primarily responsible for making the resource
<strong>Zolessi Elizalde, Flavio Rafael</strong>
<strong>Hellman, Ulf</strong>
<strong>Baz Morelli, Adriana</strong>
<strong>Arruti, C</strong>
Source
A related resource from which the described resource is derived
Biochemical and Biophysical Research Communications v.257, 1999. -- p. 480-487
Publisher
An entity responsible for making the resource available
Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes</p>
<p>(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
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DOI: 10.1006/bbrc.1999.0490
Anticuerpos
Biologia Celular
Biología molecular
Proteínas