<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:dcterms="http://purl.org/dc/terms/">
<rdf:Description rdf:about="https://riquim.fq.edu.uy/items/show/6857">
    <dcterms:title><![CDATA[<strong>Peptide derived from plant defensins: a promising 68Ga radiolabelled agent for diagnostic of infection foci in PET</strong>]]></dcterms:title>
    <dcterms:subject><![CDATA[ASPERGILLUS NIGER]]></dcterms:subject>
    <dcterms:subject><![CDATA[CEIBO]]></dcterms:subject>
    <dcterms:subject><![CDATA[CANDIDA ALBICANS]]></dcterms:subject>
    <dcterms:subject><![CDATA[PEPTIDOS]]></dcterms:subject>
    <dcterms:subject><![CDATA[STAPHYLOCOCCUS AUREUS]]></dcterms:subject>
    <dcterms:subject><![CDATA[BIBLIOGRAFIA NACIONAL QUIMICA]]></dcterms:subject>
    <dcterms:subject><![CDATA[2024]]></dcterms:subject>
    <dcterms:abstract><![CDATA[The development of new radiopharmaceuticals for the detection of hidden infection foci has great relevance for early detection and the selection of the correct treatment, particularly in immunosuppressed patients. In that sense, the labelling of antimicrobial peptides (AMPs) that are capable of binding specifically to the pathogenic microorganism which causes the infection, should provide a sufficiently specific agent, able to distinguish an infection from a sterile inflammation. Defensins are particularly interesting molecules with antimicrobial activity, the EcgDf1 defensin was identified from the genome of a Uruguayan native plant, Erythrina crista-galli, the &lsquo;Ceibo&rsquo; tree. Our group has previously reported a synthetic biologically active short analogue EcgDf21 (ERFTGGHCRGFRRRCFCTKHC) successfully labelled with 99mTc. Herein we present a shorter analogue which also preserves the &gamma;-core domain, as a pharmacophore for a potential infection detection agent. This peptide was derivatized with the bifunctional chelating agent 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) through a lysine linker in the amino-terminal group (NOTA-KGHCRGFRRRC) and radiolabelled with 68Ga ([68Ga]Ga-NOTA-K-EcgDf1(10)). The [68Ga]Ga-NOTA-K-EcgDf1(10) labelling procedure rendered a product with high radiochemical purity and stability in the labelling milieu. The Log P value indicated that the complex has a hydrophilic behaviour, confirmed by the biodistribution profile. The [68Ga]Ga-NOTA-K-EcgDf1(10) complex demonstrated specific binding to cultures of Candida albicans and Aspergillus niger. Its biodistribution showed renal elimination and low accumulation in the rest of the body. It was possible to successfully differentiate sterile inflammation from infection by PET images in nude mice with a target/non-target ratio of 3.3 for C. albicans and 3.7 for A. niger, respectively.]]></dcterms:abstract>
    <dcterms:creator><![CDATA[<strong>Osorio, Jessica</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?c3b26e04b115c427dde5cc8f9acc5f7e" target="_blank"><strong>Castro Rosas, Roberto</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?762b8fe2a16598dbbea9d81004746939aea0f840d8aa1e7a0074441ca7113eaf70593133c6037cbedaa8e8d22d78def3c697948887dc603469c21c0d05729586" target="_blank"><strong>Barraco Vega, Mariana</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?bcf54f13fbc2b4f0c0db5fa53c0d3a3799fcf2ba54fe6cf762e49f19109f684d81597cb16cc66a069f63ebfcfccd5be4ab5c160310cae3a9c42bcdf64ccd2db5" target="_blank"><strong>Reyes, Ana Laura</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?b0dab5c456275b119788a0765a192b5472042c732a55377b1855bc183f2188bb206ead86e04c40d7724c2e9747ada86c7cc4ada4db4992d83d9dd1812500edea" target="_blank"><strong>Paolino, Andrea</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?672abfaee668d8143a7b356856ae2d1b3876cb1cb1c28383aef4dbd8dbc4cdfa469351307b069733b0b4f90bb0616b9bcaaad88bf8c2b817274a4dc98aa1ae9a" target="_blank"><strong>Men&eacute;ndez, Florencia</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?672abfaee668d8143a7b356856ae2d1b3876cb1cb1c28383aef4dbd8dbc4cdfa469351307b069733b0b4f90bb0616b9bcaaad88bf8c2b817274a4dc98aa1ae9a" target="_blank"><strong>Vega-Teijido, Mauricio</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?60276f88477318edd00bb24e1f35441ae3bddd4cee93156bcdf5fa754e9a98357dc0a4c6acf0c55a15e3137839aeffe5a2f01d7800a4ff31e83380374e2b7728" target="_blank"><strong>Savio, Eduardo</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?44fdd0a91803ce0502eee9f4ffadef292fcbecefdc292eac0c76afbdce088c91cd8be39ea544d8e9e9dbafc0d21627b25fe4e21ceccb4fe0015cc1e361df511f" target="_blank"><strong>Giglio, Javier</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?218b84c2789c9c69e07f34fe382a7957b23bce652811fe0d610d4cc4548f3798c0f8890d9ff98c7f5c1ba06ec276f43552fc8d108a551f3d4572b9632eebecb0" target="_blank"><strong>Cecchetto, Gianna</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?3854b6ca9e4736c84abb19a87040ca5cdb736e955554e345f794f144edfa97713df504d3b91234778cf86d515e65c48904fd0eadb17005e5138566750a869fec" target="_blank"><strong>Ter&aacute;n, Mariella</strong></a>]]></dcterms:creator>
    <dcterms:source><![CDATA[Chemical Biology and Drug Design, v. 104, n&ordm; 1, 2024. -- e14578]]></dcterms:source>
    <dcterms:publisher><![CDATA[Wiley]]></dcterms:publisher>
    <dcterms:date><![CDATA[2024]]></dcterms:date>
    <dcterms:rights><![CDATA[<p><strong>Informaci&oacute;n sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislaci&oacute;n uruguaya protege el derecho</strong>&nbsp;de autor sobre toda creaci&oacute;n literaria, cient&iacute;fica o art&iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&oacute;n a lo establecido por el derecho com&uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong>&nbsp;La consulta de este documento queda condicionada a la aceptaci&oacute;n de las siguientes condiciones de uso: Este documento es &uacute;nicamente para usos privados enmarcados en actividades de investigaci&oacute;n y docencia. No se autoriza su reproducci&oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&oacute;n o cita de partes debe indicarse el nombre de la persona autora.</p>]]></dcterms:rights>
    <dcterms:format><![CDATA[PDF]]></dcterms:format>
    <dcterms:extent><![CDATA[11 p.]]></dcterms:extent>
    <dcterms:language><![CDATA[Ingl&eacute;s]]></dcterms:language>
    <dcterms:type><![CDATA[Art&iacute;culo]]></dcterms:type>
    <dcterms:identifier><![CDATA[10.1111/cbdd.14578]]></dcterms:identifier>
</rdf:Description><rdf:Description rdf:about="https://riquim.fq.edu.uy/items/show/6600">
    <dcterms:title><![CDATA[<strong>[99mTc]Tc-HYNIC-EcgDf21 : a defensin short analogue with potential application in infection foci imaging</strong>]]></dcterms:title>
    <dcterms:subject><![CDATA[HONGOS]]></dcterms:subject>
    <dcterms:subject><![CDATA[ASPERGILLUS NIGER]]></dcterms:subject>
    <dcterms:subject><![CDATA[CANDIDA ALBICANS]]></dcterms:subject>
    <dcterms:subject><![CDATA[CEIBO]]></dcterms:subject>
    <dcterms:subject><![CDATA[DEFENSINAS]]></dcterms:subject>
    <dcterms:subject><![CDATA[PEPTIDOS]]></dcterms:subject>
    <dcterms:subject><![CDATA[STAPHYLOCOCCUS AUREUS]]></dcterms:subject>
    <dcterms:subject><![CDATA[TRAZADORES RADIOACTIVOS]]></dcterms:subject>
    <dcterms:subject><![CDATA[TECNECIO]]></dcterms:subject>
    <dcterms:subject><![CDATA[BIBLIOGRAFIA NACIONAL QUIMICA]]></dcterms:subject>
    <dcterms:subject><![CDATA[2022]]></dcterms:subject>
    <dcterms:abstract><![CDATA[Opportunistic infections are a problem of great relevance in public health and the precise detection and localization of infection in the early stages of the dis ease is of great importance for patient management as well as cost containment. Our proposal seeks to contribute to developing a new agent that meets the needs of diagnosis and follow-up of fungal and bacterial infections, focused on the de sign of a radiotracer with the potential for recognition of hidden infection foci. Defensins are plant antimicrobial peptides that not only show activity against plant pathogens but also against human ones. A short analogue of EcgDf1 de fensin, EcgDf21d (NH2-ERFTGGHCRGFRRRCFCTKHC-COOH), was labelled through the formation of a 99mTc-HYNIC complex which was assessed for phys icochemical and biological behaviour both in vitro and in vivo. The [99mTc]Tc HYNIC-EcgDf21 labelling procedure rendered a single product with remarkably high RCP and stability in the labelling milieu. The Log p value indicated that [ 99mTc]Tc-HYNIC-EcgDf21 has a hydrophilic behaviour, confirmed by the biodis tribution profiles. The optimal uptake value was obtained for Candida albicans infection model reaching a lesion/muscle ratio of 3, this correlates with in vitro binding studies, and the lesion can be definitely observed in the scintigraphic images.]]></dcterms:abstract>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?3854b6ca9e4736c84abb19a87040ca5cdb736e955554e345f794f144edfa97713df504d3b91234778cf86d515e65c48904fd0eadb17005e5138566750a869fec" target="_blank"><strong>Ter&aacute;n, Mariella&nbsp;</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Osorio, Jessica&nbsp;</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?a2fb68a486af477aa36b46a5327480f26dc7c3c526f31621436f65951d5829b0f2c4d46b70d597d34e4a83aca96b0753195c070a6d52a4a6b6af34188553a1dd" target="_blank"><strong>Cardoso, Elena</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?cdd76ed451701e2bd1e2542e1b3b07f6a1eefa0f322ee7d761b5355fab11b59d0e6008699812d6b904ab5cefba5b3b029d6d2e55ec756b3859959c4403ee3501" target="_blank"><strong>Tejer&iacute;a, Emilia</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?b0dab5c456275b119788a0765a192b5472042c732a55377b1855bc183f2188bb206ead86e04c40d7724c2e9747ada86c7cc4ada4db4992d83d9dd1812500edea" target="_blank"><strong>Paolino, Andrea&nbsp;</strong></a>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<strong>Reyes, Ana Laura&nbsp;</strong>]]></dcterms:creator>
    <dcterms:creator><![CDATA[<a href="https://export.cvuy.uy/cv/?218b84c2789c9c69e07f34fe382a7957b23bce652811fe0d610d4cc4548f3798c0f8890d9ff98c7f5c1ba06ec276f43552fc8d108a551f3d4572b9632eebecb0" target="_blank"><strong>Cecchetto, Gianna&nbsp;</strong></a>]]></dcterms:creator>
    <dcterms:source><![CDATA[Chemical Biology and drug design, v.101, 2022]]></dcterms:source>
    <dcterms:publisher><![CDATA[Wiley]]></dcterms:publisher>
    <dcterms:date><![CDATA[2022]]></dcterms:date>
    <dcterms:rights><![CDATA[<p><strong>Informaci&oacute;n sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislaci&oacute;n uruguaya protege el derecho</strong>&nbsp;de autor sobre toda creaci&oacute;n literaria, cient&iacute;fica o art&iacute;stica, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeci&oacute;n a lo establecido por el derecho com&uacute;n y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong>&nbsp;La consulta de este documento queda condicionada a la aceptaci&oacute;n de las siguientes condiciones de uso: Este documento es &uacute;nicamente para usos privados enmarcados en actividades de investigaci&oacute;n y docencia. No se autoriza su reproducci&oacute;n con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilizaci&oacute;n o cita de partes debe indicarse el nombre de la persona autora.</p>]]></dcterms:rights>
    <dcterms:format><![CDATA[PDF]]></dcterms:format>
    <dcterms:extent><![CDATA[12 p.]]></dcterms:extent>
    <dcterms:language><![CDATA[Ingl&eacute;s]]></dcterms:language>
    <dcterms:type><![CDATA[Art&iacute;culo]]></dcterms:type>
    <dcterms:identifier><![CDATA[10.1111/cbdd.14149]]></dcterms:identifier>
</rdf:Description></rdf:RDF>
