1
25
4
-
https://riquim.fq.edu.uy/files/original/939c6f0b2749c13ea02fdcc558a642d5.pdf
c845c1000b6329c094a98acfecd6b7ea
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Title
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Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
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Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Purification and characterization of α-fucosidase from Dichostereum sordulentum 1488</strong>
Subject
The topic of the resource
GLICOSIDASAS
ENZIMAS
CARBOHIDRATOS
A-L-FUCOSA
A-FUCOSIDASA
BIBLIOGRAFIA NACIONAL QUIMICA
2024
Abstract
A summary of the resource.
Biological glycans mediate several physiological processes, thus altered glycosylation patterns can lead to different diseases such as autoimmune, infectious, chronic anti-inflammatory diseases, or even cancer. In fact, alterations in fucosylation in either N- or O-glycans are among the most frequent changes in glycosylation patterns associated with cancer. Therefore, elucidation of the role of glycoconjugate glycans is essential for understanding the development of pathologies where they are involved. In this sense glycosidases are excellent tools, since they catalyse the selective removal of sugar residues, allowing the evaluation of changes in their biological role due to glycan removal. This work describes the purification and characterization of a α-fucosidase from the fungus Dichostereum sordulentum 1488. It is a homodimer with a molecular weight of 214 kDa and optimum pH and temperature of 4.0 and 70 ◦C respectively. It has a KM of 0.27 mM and VMax of 3.3 μmoles PNP/ min per mg for the substrate p-nitrophenyl-α-L-fucopyranoside, showing a substrate inhibition profile. It showed high specificity for the hydrolysis of fucose linked by α-(1,2) bonds. The identification, purification, and characterization of this new α-fucosidase is highly relevant for enlarging the availability of glycosidases for use as tools for glycan elucidation.
Creator
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<a href="https://export.cvuy.uy/cv/?89f4f95ef44a6b6cf3cc08494680b267"><strong>Herrera, Lorena</strong></a>
<a href="https://export.cvuy.uy/cv/?441488c6d1acc8438d8713846d7f670b"><strong>Cedrés, María Eugenia</strong></a>
<a href="https://export.cvuy.uy/cv/?a7dc62e1b1bbd449b3ac8472794ba2e52c18f2d4c52e9debc1b69bc3869e0f2eee9e4150d80c79f75a9876faa56a6b16a3a194119f3a19e8ff051ce749292a53"><strong>Rodríguez Bonnecarrére, Paula</strong></a>
<a href="https://export.cvuy.uy/cv/?8c80c1ae8b95cb6b8bc4b6ce3a8d3a39b297d1bd27a3804c09be97adcbe35a3741bd28180c0446feac51238afbedb3cf67010e8ed6fa462a13806c083463ea87"><strong>Giacomini, Cecilia</strong></a>
Source
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Carbohydrate Research, v. 545, nº 1, 2024. -- e109278
Publisher
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Elsevier
Date
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2024
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<div class="element-text">
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
</div>
Format
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PDF
Extent
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8 p.
Language
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Inglés
Type
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Artículo
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10.1016/j.carres.2024.109278
a-fucosidasa
a-L-fucosa
Carbohidratos
Enzimas
Glicosidasas
-
https://riquim.fq.edu.uy/files/original/cc537e75a05944079c65cdbb3f70a50f.pdf
0ebebc2e46cd599f97051dd7aefd7031
https://riquim.fq.edu.uy/files/original/0b84e89f5f4226451bee9d645c4934c3.gif
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
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8
Channels
3
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150
Width
113
Dublin Core
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles : A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates</strong>
Subject
The topic of the resource
INMOVILIZACION DE ENZIMAS
GLICOSIDASAS
ANALISIS GLICOMICO
BIBLIOGRAFIA NACIONAL QUIMICA
2018
Abstract
A summary of the resource.
Glycans present in biological glycoconjugates have several structural and functional roles. Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment. Immobilized glycosidases are powerful tools for glycan analysis, as they are able to remove specific carbohydrates without altering the protein structure. Here we describe the individual immobilization of Aspergillus oryzae β-galactosidase and Canavalia ensiformis α-mannosidase onto agarose and silica magnetic nanoparticles activated with cyanate ester groups. High immobilization yields (70–90%) were achieved, keeping above 60% of its original activity. Immobilized glycosidases were effective in the selective deglycosylation of model glycoproteins and a Fasciola hepatica lysate, evidenced by a decrease in specific lectin recognition of 40–50% after enzymatic deglycosylation. Immobilized glycosidases were reused for several deglycosylation cycles without loss of effectiveness. Their use was extended to the elucidation of the glycan role of native glycoconjugates. A decrease in the recognition of lactoferrin treated with α-mannosidase by a C-type lectin receptor, DC-SIGN was found. Also the specific deglycosylation of a F. hepatica lysate demonstrated the relevance of mannosylated glycans in the induction of Th2/Treg immune responses during the infection. Our results show successful immobilization of specific glycosidases in nano-supports and validate their utility to identify glycans biological functions.
Creator
An entity primarily responsible for making the resource
<strong>Rodríguez, Ernesto</strong>
<strong>Francia, Karen.</strong>
<strong>Brossard, Natalie</strong>
<strong>García Vallejo, Juan J.</strong>
<strong>Kalay, Hakan</strong>
<strong>van Kooyk, Ivette</strong>
<strong>Freire, Teresa</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=3c8a423235a59fcbc901eec52b2b7794" target="_self"><strong>Giacomini, Cecilia</strong></a>
Source
A related resource from which the described resource is derived
Enzyme and Microbial Technology v. 117, 2018. -- p. 45-55
Publisher
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Elsevier
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong> La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong> ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
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PDF
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Artículo
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DOI: 10.1016/j.enzmictec.2018.05.012
Analisis Glicómico
Glicosidasas
Inmovilizacion de enzimas
-
https://riquim.fq.edu.uy/files/original/49f4ce29683eae67396254819a3e48df.pdf
7b1d7e6dd398ef8c59e9c62d930cef3b
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Omeka Image File
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8
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340
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240
Dublin Core
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>General Method for the Synthesis of -Conduritol C and Analogs from Chiral Cyclohexadienediol Scaffolds</strong>
Subject
The topic of the resource
GLICOSIDASA
GLICOSIDASA-INHIBIDORES
CONDURITOL C
BIOCATALISIS
ESTRUCTURA CRISTALINA
BIBLIOGRAFIA NACIONAL QUIMICA
2018
Abstract
A summary of the resource.
An efficient and facile general method for the synthesis of conduritol C analogs, taking advantage of an enantioselective biocatalysis process of monosubstituted benzenes, is described. The absolute stereochemical patterns of the target molecules (−)-conduritol C, (−)-bromo-conduritol C, and (−)-methyl-conduritol C were achieved by means of chemoenzymatic methods. The stereochemistry present at the homochiral cyclohexadiene-cis-1,2-diols derived from the arene biotransformation and the enantioselective ring opening of a non-isolated vinylepoxide derivative permitted the absolute configuration of the carbon bearing the hydroxyl groups at the target molecules to be established. All three conduritols and two intermediates were crystallized, and their structures were confirmed by X-ray diffraction. The three conduritols and intermediates were isostructural. The versatility of our methodology is noteworthy to expand the preparation of conduritol C analogs starting from toluene dioxygenase (TDO) monosubstituted arene substrates.
Creator
An entity primarily responsible for making the resource
<strong>Tibhe, Gaurao D.</strong>
<strong>Macías, Mario A</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=0b6d7b4c1f21766e03e6c9354d672f8d" target="_self"><strong>Schapiro, Valeria</strong></a>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8b1a957170f0b5988597d2cc3d6f732b" target="_self"><strong>Suescun, Leopoldo</strong></a>
<a title="Curriculum Vitae" href="ttp://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=1ad085b4305ec8d8997ce8a565b947d4" target="_self"><strong>Pandolfi Graziosi, Enrique.</strong></a>
Source
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Molecules v. 23, 2018. --9p.--e1653
Publisher
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MDPI
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor </strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Articulo
Identifier
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DOI: 10.3390/molecules23071653
Conduritol C
Glicosidasa-Inhibidores
Glicosidasas
-
https://riquim.fq.edu.uy/files/original/34eede4b5b095ce112720a0237b337c7.pdf
47a04f8f934ca1a865868e503e4e507f
https://riquim.fq.edu.uy/files/original/611675883a1cc2ad29de65ecc6accdac.jpg
d4dce153448482eb95985c40061baaf4
Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
Channels
3
Height
142
Width
110
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Enzymatic synthesis of 3-aminopropyl-1-O-β-Dgalactopyranoside catalyzed by Aspergillus oryzae β-galactosidase</strong>
Subject
The topic of the resource
ENZIMAS
GALACTOSIDASAS
GLICOSIDASAS
BIBLIOGRAFIA NACIONAL QUIMICA
2015
Abstract
A summary of the resource.
Glycosidases represent excellent green chemistry alternatives as catalysts for the synthesis of glycosides, and in particular their stereoselectivity allows the production of anomerically pure glycosides, in only one reaction step using mild reaction conditions. Here, we report the enzymatic synthesis and structural characterization of 3-aminopropyl-1-O-β-D-galactopyranoside. Optimal reaction conditions for the transgalactosylation reaction were 100 mM lactose, 500 mM 3-amino-1-propanol and 24 h of incubation at 50 °C with 6 U/mL of β-galactosidase from Aspergillus oryzae. The fact that the synthesis of 1-propyl-2-O-β-D-galactopyranoside using 1-amino-2-propanol as acceptor was not achieved, and that N-glycoside formation was not observed, confirms the selectivity of β-galactosidase for the synthesis of O-glycosides, and particularly for primary alcohols. The synthesized galactosides were evaluated for their ability to interact with bovine spleen galectin-1 (Gal-1) by using the hemagglutination inhibition assay; results demonstrated that 3-aminopropyl-1-O-β-D-galactopyranoside may be considered as a functionalized galactose moiety more than an efficient Gal-1 inhibitor. The proposed approach constitutes a promising tool for the generation of glycopolymers and glyconanoparticles with potential applications in the development of biosensors as well as construction blocks in chemical synthesis.
Creator
An entity primarily responsible for making the resource
<strong>Porciúncula González, Cecilia</strong>
<strong>Rodríguez, Ernesto</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=1502d6d114aace9e148166840e515f38" target="_blank"><strong>Soulé, Silvia</strong></a>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=729d3d9bb07d7be0ba5e4dc762c01e29" target="_blank"><strong>Franco Fraguas, Laura</strong></a>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=586cda0b970a37c5463af91ae8481fed" target="_blank"><strong>Brena, Beatriz M.</strong></a>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=3c8a423235a59fcbc901eec52b2b7794" target="_blank"><strong>Giacomini, Cecilia</strong></a>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=da4f071f890fec490234707830d5f695" target="_blank"><strong>Irazoqui, Gabriela</strong></a>
Source
A related resource from which the described resource is derived
Biocatalysis and Biotransformation v. 33, no. 4, 2015. -- p. 197-207
Publisher
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Taylor Francis
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong><br /> <br /> (Por favor lea este aviso antes de abrir los documentos u objetos)<br /> <br /> <strong>La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes<br /> <br /> (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)<br /> <br /> <strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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DOI: 10.3109/10242422.2015.1095678
Enzimas
Galactosidasas
Glicosidasas