1 25 9 https://riquim.fq.edu.uy/files/original/8dba11a276e182ce6357dc5d617f0259.pdf ea68912b5d455b1ca8a05541f84ea604 https://riquim.fq.edu.uy/files/original/0c1b70ecb37003bcc2c92e61a5418885.jpg c2ad83a8bd076ff5d79c106b37c5b026 Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 155 Width 187 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data PDF Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>A highly sensitive nanobody-based immunoassay detecting SARS-CoV-2 nucleocapsid protein using all-recombinant reagents</strong> Subject The topic of the resource NANOCUERPOS COVID-19 PROTEINA DE LA NUCLEOCAPSIDE BIBLIOGRAFIA NACIONAL QUIMICA 2023 Abstract A summary of the resource. Antigen tests have been crucial for managing the COVID-19 pandemic by identifying individuals infected with SARS-CoV-2. This remains true even after immunity has been widely attained through natural infection and vaccination, since it only provides moderate protection against transmission and is highly permeable to the emergence of new virus variants. For this reason, the widespread availability of diagnostic methods is essential for health systems to manage outbreaks effectively. In this work, we generated nanobodies to the virus nucleocapsid protein (NP) and after an affinity-guided selection identified a nanobody pair that allowed the detection of NP at sub-ng/mL levels in a colorimetric two-site ELISA, demonstrating high diagnostic value with clinical samples. We further modified the assay by using a nanobody-NanoLuc luciferase chimeric tracer, resulting in increased sensitivity (detection limit = 61 pg/mL) and remarkable improvement in diagnostic performance. The luminescent assay was finally evaluated using 115 nasopharyngeal swab samples. Receiver Operating Characteristic (ROC) curve analysis revealed a sensitivity of 78.7% (95% confidence interval: 64.3%-89.3%) and specificity of 100.0% (95% confidence interval: 94.7%-100.0%). The test allows the parallel analysis of a large number of untreated samples, and fulfills our goal of producing a recombinant reagent-based test that can be reproduced at low cost by other laboratories with recombinant expression capabilities, aiding to build diagnostic capacity. Creator An entity primarily responsible for making the resource <a href="https://export.cvuy.uy/cv/?5246a5ca81aeb900b0acc0265c03abd2" target="_blank"><strong>Segovia de los Santos, Paula</strong></a> <a href="https://export.cvuy.uy/cv/?86fbaf91aa923439a4e294f1925a27ca" target="_blank"><strong>Padula Roca, Carolina</strong></a> <strong>Simon, Ximena</strong> <strong>Echaides, César</strong> <a href="https://export.cvuy.uy/cv/?1c1f29bd240d37882295d2531e2cfdd7bed8462cd28f84bbf32453c0ac9c793ec306557fbabc459cfa8e8564b47d63924c13a8ea78e6df85af1afa01363bc336" target="_blank"><strong>Lassabe, Gabriel</strong></a> <a href="https://export.cvuy.uy/cv/?f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20" target="_blank"><strong>González Sapienza, Gualberto</strong></a> Source A related resource from which the described resource is derived Frontiers in immunology, v.14, 2023 Publisher An entity responsible for making the resource available Frontiers Date A point or period of time associated with an event in the lifecycle of the resource 2023 Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> Format The file format, physical medium, or dimensions of the resource PDF Extent The size or duration of the resource. 12 p. Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context 10.3389/fimmu.2023.1220477 Covid-19 Nanocuerpos Proteína de la nucleocápside https://riquim.fq.edu.uy/files/original/81bee82c873b5a6eeefc36c31f4dd596.pdf c845c1000b6329c094a98acfecd6b7ea https://riquim.fq.edu.uy/files/original/32165fdea9a53a2c3a6a20af2ec75a93.jpg aa2da6a49295df971b18e68d3651fcde Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 155 Width 116 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data PDF Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>A highly sensitive nanobody-based ELISA for bovine β-lactoglobulin to classified donated human milk destined to susceptible newborns</strong> Subject The topic of the resource NANOCUERPOS BETA-LACTOGLOBULINA LECHE MATERNA BANCOS DE LECHE BIBLIOGRAFIA NACIONAL QUIMICA 2023 Abstract A summary of the resource. The β-lactoglobulin protein from cow’s milk is a common allergen present in human milk that leads to serious allergic reactions in susceptible infants. This creates a serious safety issue in Human Milk Banks, and the best practice to assure the safety of susceptible newborns would be to classify the donated human milk according to its content of β-lactoglobulin. To provide a simple and fast assay to detect this molecule in human milk we developed a nanobody-based sandwich ELISA. The capture and detection nanobodies were isolated by phage display from a llama immunized with the antigen using a sequential selection strategy for antibody pair iden tification. This allowed to attain a highly sensitive test with a limit of quantitation of 40 pg/mL and extended lineal range of up to 3000 pg/mL, showing an excellent performance with human milk. For these characteristics, the ELISA developed might be useful for the classification of donated human milk according to their β-lacto globulin content, improving the quality of milk destined to susceptible newborns. Creator An entity primarily responsible for making the resource <a href="https://export.cvuy.uy/cv/?71934076182f14281ef9fe7e2c7dc381a644339885c1cf0c38160f7dd9fd90b916148bcc01ec448981888590b0b3084a814a46e59aff2122c09d57f1cf176f22" target="_blank"><strong>Rodríguez Camejo, Claudio</strong></a> <a href="https://export.cvuy.uy/cv/?2151545e1e2bcf5a1aed18f0fcbc86f8f572989a1ff4170a7081b84aaee68c3f0c594002567678df9a2ad53f09c41af0654f6be4d057bda841a03a8994b48e31" target="_blank"><strong>Delfin Riela, Triana</strong></a> <a href="https://export.cvuy.uy/cvsni/?urlId=0714ab9cf73af9870ec8809b325b44115e7a8c32a7bffc5b0260762ba5347b07fa4dc1efff263fa6245889f68b031f22fc09f7dd37fb84c74004165d1dca6005&convocatoria=21&formato=html" target="_blank"><strong>Rossotti, Martín A.</strong></a> <strong>Puyol, Arturo</strong> <strong>Echaides, César</strong> <a href="https://export.cvuy.uy/cv/?e48336f406343f48c858e7403983e63cc46d4bcc33267754f0e951102827dcb148a4da125f875269753f952843d446507c27d521c2053a1d384354c62d730f2c" target="_blank"><strong>Hernández, Ana</strong></a> <strong>González Sapienza, Gualberto</strong> Source A related resource from which the described resource is derived Food Control, v.153, 2023. --e109910 Publisher An entity responsible for making the resource available Elsevier Date A point or period of time associated with an event in the lifecycle of the resource 2023 Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> Format The file format, physical medium, or dimensions of the resource PDF Extent The size or duration of the resource. 8 p. Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context 10.1016/j.foodcont.2023.109910 Bancos de leche Beta-Lactoglobulina Leche materna Nanocuerpos https://riquim.fq.edu.uy/files/original/8eb692d27954e1f77f4516fcbab11c06.pdf c845c1000b6329c094a98acfecd6b7ea https://riquim.fq.edu.uy/files/original/3ddf456d3ac0793f43361b76c691e0dc.PNG 3dd78a822b0144d37010d3aeb0f5e16b Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Height 203 Width 150 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data PDF Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>Local delivery of optimized nanobodies targeting the PD-1/PD-L1 axis with a self-amplifying RNA viral vector induces potent antitumor responses</strong> Subject The topic of the resource NANOCUERPOS VIRUS DEL BOSQUE SEMLIKI SFV ALFAVIRUS PD-1 PD-L1 INMUNOTERAPIA CANCER BIBLIOGRAFIA NACIONAL QUIMICA 2023 Abstract A summary of the resource. Despite the success of immune checkpoint blockade for cancer therapy, many patients do not respond adequately. We aimed to improve this therapy by optimizing both the antibodies and their delivery route, using small monodomain antibodies (nanobodies) delivered locally with a self-amplifying RNA (saRNA) vector based on Semliki Forest virus (SFV). We generated nanobodies against PD-1 and PD-L1 able to inhibit both human and mouse interactions. Incorporation of a dimerization domain reduced PD-1/PD-L1 IC50 by 8- and 40-fold for anti- PD-L1 and anti-PD-1 nanobodies, respectively. SFV viral particles expressing dimeric nanobodies showed a potent antitumor response in the MC38 model, resulting in >50% complete regressions, and showed better therapeutic efficacy compared to vectors expressing conventional antibodies. These effects were also observed in the B16 melanoma model. Although a short-term expression of nanobodies was observed due to the cytopathic nature of the saRNA vector, it was enough to generate a strong proinflammatory response in tumors, increasing infiltration of NK and CD8+ T cells. Delivery of the SFV vector expressing dimeric nanobodies by local plasmid electroporation, which could be more easily translated to the clinic, also showed a potent antitumor effect. Creator An entity primarily responsible for making the resource <strong>Silva Pilipich, Noelia</strong> <strong>Blanco, Ester</strong> <strong>Lozano, Teresa</strong> <strong>Martisova, Eva</strong> <strong>Igea, Ana</strong> <strong>Herrador-Cañete, Guillermo</strong> <strong>Ballesteros-Briones, María Cristina</strong> <strong>Gorraiz, Marta</strong> <strong>Sarrión, Patricia</strong> <strong>González Sapienza, Gualberto</strong> <strong>Lasarte, Juan José</strong> <strong>Vanrell, Lucía</strong> <strong>Smerdou, Cristian</strong> Source A related resource from which the described resource is derived Cancer Letters v. 561, 2023. -- e216139 Publisher An entity responsible for making the resource available Elsevier Date A point or period of time associated with an event in the lifecycle of the resource 2023 Rights Information about rights held in and over the resource <div class="element-text"> <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> </div> Format The file format, physical medium, or dimensions of the resource PDF Extent The size or duration of the resource. 13 p. Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context 10.1016/j.canlet.2023.216139 Alfavirus Cáncer Inmunoterapia Nanocuerpos PD-1 PD-L1 SFV Virus del Bosque Semliki https://riquim.fq.edu.uy/files/original/6c47691a904e90d5cb4430742e6e5f17.pdf 26eae7e0c2a58a43c61fe0694186c6ec https://riquim.fq.edu.uy/files/original/a6dc2797eb95335c793e1e18722746f2.png 9062914763101fddffef314155415bf2 Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Height 155 Width 116 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data PDF Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>Induction of antigen-specific tolerance by nanobody–antigen adducts that target class-II major histocompatibility complexes</strong> Subject The topic of the resource ANTIGENOS HISTOCOMPATIBILIDAD NANOCUERPOS ENFERMEDADES AUTOINMUNES BIBLIOGRAFIA NACIONAL QUIMICA 2021 Abstract A summary of the resource. <p>The association of autoimmune diseases with particular allellic products of the class-II major histocompatibility complex<br />(MHCII) region implicates the presentation of the offending self-antigens to T cells. Because antigen-presenting cells are<br />tolerogenic when they encounter an antigen under non-inflammatory conditions, the manipulation of antigen presentation may induce antigen-specific tolerance. Here, we show that, in mouse models of experimental autoimmune encephalomyelitis, type 1 diabetes and rheumatoid arthritis, the systemic administration of a single dose of nanobodies that recognize MHCII molecules and conjugated to the relevant self-antigen under non-inflammatory conditions confers long-lasting protection against these diseases. Moreover, co-administration of a nanobody–antigen adduct and the glucocorticoid dexamethasone, conjugated to the nanobody via a cleavable linker, halted the progression of established experimental autoimmune encephalomyelitis in symptomatic mice and alleviated their symptoms. This approach may represent a means of treating autoimmune conditions.</p> Creator An entity primarily responsible for making the resource <strong>Pishesha, Novalia</strong> <strong>Harmand, Thibault</strong> <strong>Smeding, Liyan Y.</strong> <strong>Ma, Weiyi</strong> <strong>Ludwing, Leif S.</strong> <strong>Janssen, Robine</strong> <strong>Islam, Ashraful</strong> <strong>Xie, Yushu J.</strong> <strong>Fang, Tao</strong> <strong>McCaul, Nicholas</strong> <strong>Pinney III, Wiliam</strong> <strong>Sugito, Harun R.</strong> <a href="https://exportcvuy.anii.org.uy/cvsni/?urlId=0714ab9cf73af9870ec8809b325b44115e7a8c32a7bffc5b0260762ba5347b07fa4dc1efff263fa6245889f68b031f22fc09f7dd37fb84c74004165d1dca6005&convocatoria=21&formato=html" target="_blank"><strong>Rossoti, Martin A.</strong></a> <a href="https://exportcvuy.anii.org.uy/cv/?f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20" target="_blank"><strong>González Sapienza, Gualberto</strong></a> <strong>Ploegh, Hidde L.</strong> Source A related resource from which the described resource is derived Nature Biomedical Engineering, 2021. Publisher An entity responsible for making the resource available Springer Date A point or period of time associated with an event in the lifecycle of the resource 2021 Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context <span id="docs-internal-guid-80b53838-7fff-331c-3ee1-4cf13e92c83e"><span>10.1038/s41551-021-00738-5  </span></span> Antígenos Enfermedades autoinmunes Histocompatibilidad Nanocuerpos https://riquim.fq.edu.uy/files/original/6ab8df8814eb49152a0e3379b45c4487.pdf b9b6ba7ffc452d52cf936763b24ec2e5 https://riquim.fq.edu.uy/files/original/ae514cf095ad8f969a6d5335171b0afe.jpg a259be0f783a969362a223b745883ca8 Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 154 Width 152 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data Pdf <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>Highly Sensitive Detection of Zika Virus Nonstructural Protein 1 in Serum Samples by a Two-Site Nanobody ELISA</strong> Subject The topic of the resource ZIKA-VIRUS <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> INMUNOENSAYO ELISA <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> NANOCUERPOS <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> BIBLIOGRAFIA NACIONAL QUIMICA <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> 2020 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Abstract A summary of the resource. The Zika virus was introduced in Brazil in 2015 and, shortly after, spread all over the Americas. Nowadays, it remains present in more than 80 countries and represents a major threat due to some singularities among other flaviviruses. Due to its easy transmission, high percentage of silent cases, the severity of its associated complications, and the lack of prophylactic methods and e�ective treatments, it is essential to develop reliable and rapid diagnostic tests for early containment of the infection. Nonstructural protein 1 (NS1), a glycoprotein involved in all flavivirus infections, is secreted since the beginning of the infection into the blood stream and has proven to be a valuable biomarker for the early diagnosis of other flaviviral infections. Here, we describe the development of a highly sensitive nanobody ELISA for the detection of the NS1 protein in serum samples. Nanobodies were selected from a library generated from a llama immunized with Zika NS1 (ZVNS1) by a two-step high-throughput screening geared to identify the most sensitive and specific nanobody pairs. The assay was performed with a sub-ng/mL detection limit in the sera and showed excellent reproducibility and accuracy when validated with serum samples spiked with 0.80, 1.60, or 3.10 ng/mL of ZVNS1. Furthermore, the specificity of the developed ELISA was demonstrated using a panel of flavivirus’ NS1 proteins; this is of extreme relevance in countries endemic for more than one flavivirus. Considering that the nanobody sequences are provided, the assay can be reproduced in any laboratory at low cost, which may help to strengthen the diagnostic capacity of the disease even in low-resource countries. <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Creator An entity primarily responsible for making the resource <strong>Delfin Riela, Triana</strong> <strong>Rossotti, Martín</strong> <strong>Alvez-Rosado, Romina</strong> <strong>Leizagoyen, Carmen</strong> <strong>González-Sapienza, Gualberto</strong> Source A related resource from which the described resource is derived Biomolecules v. 10, 2020. -- p. 1-14.--e 1652 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Publisher An entity responsible for making the resource available MDPI <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Date A point or period of time associated with an event in the lifecycle of the resource 2020 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong> (Por favor lea este aviso antes de abrir los documentos u objetos)<strong> La legislación uruguaya protege el derecho de autor </strong>sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006) ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Format The file format, physical medium, or dimensions of the resource Pdf <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Language A language of the resource Inglés <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Type The nature or genre of the resource Artículo <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Identifier An unambiguous reference to the resource within a given context doi:10.3390/biom10121652 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Inmumoensayo Elisa Nanocuerpos Zika-Virus https://riquim.fq.edu.uy/files/original/40d2c6339c4632d93d591b1c66603260.pdf 3afe8c00580c935879a75468669acf26 https://riquim.fq.edu.uy/files/original/88d0476073c5bee14b07ad15bb1e5f98.jpg 95b6dfc24ee8013cafa405e0becf9e3c Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 33 Width 155 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data Pdf <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>Long-Term Systemic Expression of a Novel PD-1 Blocking Nanobody from an AAV Vector Provides Antitumor Activity without Toxicity</strong> Subject The topic of the resource INMUNOTERAPIA <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> TERAPIA DE GENES <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> CANCER <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> NANOCUERPOS <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> ACTIVIDAD ANTICANCERIGENA <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> BIBLIOGRAFIA NACIONAL QUIMICA <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> 2020 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Creator An entity primarily responsible for making the resource <strong>Silva-Pilipich, Noelia</strong> <strong>Martisova, Eva</strong> <strong>Ballesteros Briones, María Cristina</strong> <strong>Hervas-Stubbs, Sandra</strong> <strong>Casares, Noelia</strong> <strong>González Sapienza, Gualberto</strong> <strong>Smerdou, Cristian</strong> <strong>Vanrell, Lucía</strong> Source A related resource from which the described resource is derived Biomedicines v. 8, 2020. -- p. 1-19.--e562 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Publisher An entity responsible for making the resource available MDPI <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Date A point or period of time associated with an event in the lifecycle of the resource 2020 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong> (Por favor lea este aviso antes de abrir los documentos u objetos)<strong> La legislación uruguaya protege el derecho de autor </strong>sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006) ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Format The file format, physical medium, or dimensions of the resource Pdf <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Language A language of the resource Inglés <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Type The nature or genre of the resource Artículo <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Identifier An unambiguous reference to the resource within a given context doi:10.3390/biomedicines8120562 <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> <div id="sconnect-is-installed" style="display: none;">2.11.0.0</div> Actividad anticancerigena Cáncer Inmunoterapia Nanocuerpos Terapia de genes https://riquim.fq.edu.uy/files/original/36bc6bfe98bc8880cae3e77f1fb3827f.pdf 381081c6197b4d45a931a88b922a3eeb https://riquim.fq.edu.uy/files/original/d96596c851d184bb3947cf13377bbc1b.gif 15608f0af4774ac45e3acf534e95c93b Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 150 Width 114 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data PDF Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Structure and specificity of several triclocarban-binding single domain camelid antibody fragments Subject The topic of the resource NANOCUERPOS ESTRUCTURA CRISTALINA TRICLOCARBAN BIBLIOGRAFIA NACIONAL QUIMICA 2019 Abstract A summary of the resource. The variable VHH domains of camelid single chain antibodies have been useful in numerous biotechnology applications due to their simplicity, biophysical properties, and abilities to bind to their cognate antigens with high affinities and specificity. Their interactions with proteins have been well-studied, but considerably less work has been done to characterize their ability to bind haptens. A high-resolution structural study of three nanobodies (T4, T9, and T10) which have been shown to bind triclocarban (TCC, 3-(4-chlorophenyl)-1-(3,4-dichlorophenyl)urea) with near-nanomolar affinity shows that binding occurs in a tunnel largely formed by CDR1 rather than a surface or lateral binding mode seen in other nanobody-hapten interactions. Additional significant interactions are formed with a non-hypervariable loop, sometimes dubbed "CDR4". A comparison of apo and holo forms of T9 and T10 shows that the binding site undergoes little conformational change upon binding of TCC. Structures of three nanobody-TCC complexes demonstrated there was not a standard binding mode. T4 and T9 have a high degree of sequence identity and bind the hapten in a nearly identical manner, while the more divergent T10 binds TCC in a slightly displaced orientation with the urea moiety rotated approximately 180° along the long axis of the molecule. In addition to methotrexate, this is the second report of haptens binding in a tunnel formed by CDR1, suggesting that compounds with similar hydrophobicity and shape could be recognized by nanobodies in analogous fashion. Structure-guided mutations failed to improve binding affinity for T4 and T9 underscoring the high degree of natural optimization. Creator An entity primarily responsible for making the resource <strong>Tabares da Rosa, Sofía</strong> <strong>Wogulis, Linda A.</strong> <strong>Wogulis, Mark D.</strong> <a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20&formato=pdf&convocatoria=21" target="_self"><strong>González Sapienza, Gualberto.</strong></a> <strong>Wilson, David K.</strong> Source A related resource from which the described resource is derived Journal of Molecular Recognition v. 32, no. 1, 2019. -- p. 1-9.--e2755 Publisher An entity responsible for making the resource available Wiley Date A point or period of time associated with an event in the lifecycle of the resource 2019 Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong> La legislación uruguaya</strong> protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora</p> Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context DOI: 10.1002/jmr.2755 Estructuras Cristalina Nanocuerpos Triclocarban https://riquim.fq.edu.uy/files/original/9bfe36056199eea80f4097fa160879a5.pdf 0ebebc2e46cd599f97051dd7aefd7031 https://riquim.fq.edu.uy/files/original/6bc6208a494ee99d06d47f6478d3a2e9.jpg 5fccbb5460217554af5ae36efc98ca4f Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 153 IPTC Array a:4:{s:7:"caption";s:72:"no spine minimum. full size. Editor: Nella JEM: Esther RTP: Erin Dalton";s:6:"byline";s:15:"wardsdesign.com";s:12:"byline_title";s:6:"ancham";s:11:"object_name";s:20:"Analytical Chemistry";} IPTC String caption:no spine minimum. full size. Editor: Nella JEM: Esther RTP: Erin Dalton byline:wardsdesign.com byline_title:ancham object_name:Analytical Chemistry Width 115 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Original Format If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data PDF Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>Oriented functionalization of magnetic beads with in vivo biotinylated nanobodies for rapid MALDI-TOF ultrasensitive quantitation of microcystins in biological samples</strong> Subject The topic of the resource CIANOTOXINAS NANOCUERPOS BIBLIOGRAFIA NACIONAL QUIMICA 2019 Abstract A summary of the resource. Here we present a new analytical method where immunoconcentration of the analyte is coupled to quantitative matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) analysis allowing in minutes the identification and highly sensitive quantitation of microcystins (MCs) as model targets. The key element is a site-specific in vivo biotinylated nanobody of broad cross-reactivity with microcystins. The single biotin moiety at the C-terminus and the small size of the nanobody (15 kDa) enable its oriented and tightly packed immobilization on magnetic beads, providing a highly efficient capture of the toxin. The binding capacity of the bioadsorbent is partially loaded with an easily synthesized internal standard for MS quantitation. After capture, the beads are directly dispensed on the MALDI-TOF MS target enabling the identification and sensitive quantitation of the microcystin (MC) congeners. Since salts and contaminants are removed during the concentration step, no cleanup or other sample treatments are needed. The method was validated with a large number of water and serum samples with excellent precision and recovery at quantitation limits of 0.025 μg/L of MC. Creator An entity primarily responsible for making the resource <a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=551c5710da6933c1089ec0d5955dcdc6f8139412c73650c95b82a2b3b02388a3247ceadce7333c784ca076abeafe679c38b5f2272687b8ab5cc950f03394f2a7&formato=pdf&convocatoria=21" target="_self"><strong>Pírez-Schirmer, Macarena</strong></a> <a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=76fda5e876d7838a885399f5cef02e0d379f384ffe6daa3cb23af35c02ec6f7f1e120c2bddda0b94b93ca0683d4b038a08132b4d8ad1bbe4dab743b47bb1df5e&formato=pdf&convocatoria=21" target="_self"><strong>Brena, Beatriz M.</strong></a> <a title="Curriculum Vitae" href="https://exportcvuy.anii.org.uy/CvEstatico/?urlId=f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20&formato=pdf&convocatoria=21" target="_self"><strong>González Sapienza, Gualberto.</strong></a> Source A related resource from which the described resource is derived Analytical Chemistry  v. 91, no. 15, 2019. --p. 9925-9931 Publisher An entity responsible for making the resource available American Chemical Society Date A point or period of time associated with an event in the lifecycle of the resource 2019 Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context DOI: 10.1021/acs.analchem.9b01596 Cianotoxinas Nanocuerpos https://riquim.fq.edu.uy/files/original/63a6eb45cdca8f40b9827abfab8a29e6.pdf 0ebebc2e46cd599f97051dd7aefd7031 https://riquim.fq.edu.uy/files/original/548a7b420edef0ed80bb9716c4717894.jpg 50703dba2ca5e0ff79aad66bb0e58961 Omeka Image File The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files. Bit Depth 8 Channels 3 Height 256 IPTC Array a:2:{s:6:"byline";s:4:"tech";s:11:"object_name";s:20:"KMAB_7_05_COVER.indd";} IPTC String byline:tech object_name:KMAB_7_05_COVER.indd Width 200 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bibliografía Nacional Química Description An account of the resource Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha. Contributor An entity responsible for making contributions to the resource Departamento de Documentación y Biblioteca RIQUIM Document A resource containing textual data. Note that facsimiles or images of texts are still of the genre text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource <strong>Increasing the potency of neutralizing single-domain antibodies by functionalization with a CD11b/CD18 binding domain</strong> Subject The topic of the resource ENSAYO INMUNO ENZIMATICO INMUNOTERAPIA NANOCUERPOS ANTICUERPOS TERAPEUTICOS BIBLIOGRAFIA NACIONAL QUIMICA 2015 Abstract A summary of the resource. Recombinant single domain antibodies (nanobodies) constitute an attractive alternative for the production of neutralizing therapeutic agents. Their small size warrants rapid bioavailability and fast penetration to sites of toxin uptake, but also rapid renal clearance, which negatively affects their performance. In this work, we present a new strategy to drastically improve the neutralizing potency of single domain antibodies based on their fusion to a second nanobody specific for the complement receptor CD11b/CD18 (Mac-1). These bispecific antibodies retain a small size (~30 kDa), but acquire effector functions that promote the elimination of the toxin-immunocomplexes. The principle was demonstrated in a mouse model of lethal toxicity with tetanus toxin. Three anti-tetanus toxin nanobodies were selected and characterized in terms of overlapping epitopes and inhibition of toxin binding to neuron gangliosides. Bispecific constructs of the most promising monodomain antibodies were built using anti Mac-1, CD45 and MHC II nanobodies. When co-administered with the toxin, all bispecific antibodies showed higher toxin-neutralizing capacity than the monomeric ones, but only their fusion to the anti-endocytic receptor Mac-1 nanobody allowed the mice to survive a 10-fold lethal dose. In a model of delayed neutralization of the toxin, the anti- Mac-1 bispecific antibodies outperformed a sheep anti-toxin polyclonal IgG that had shown similar neutralization potency in the co-administration experiments. This strategy should have widespread application in the development of nanobody-based neutralizing therapeutics, which can be produced economically and more safely than conventional antisera. Creator An entity primarily responsible for making the resource <strong>Rossotti, Martín</strong> <a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=67c04f6a200d5de027ddb6804d7bae5f" target="_blank"><strong>González Techera, Andrés</strong></a> <strong>Guarnaschelli, Julio</strong> <strong>Yim, Lucia</strong> <strong>Camacho, Ximena</strong> <strong>Fernández Silveira, Marcelo.</strong> <strong>Cabral, Pablo</strong> <strong>Leizagoyen, Carmen</strong> <strong>Chabalgoity Jose A.</strong> <a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=7ee0def1187be47e0710e877d9768b5a" target="_blank"><strong>González-Sapienza, Gualberto G</strong></a> Source A related resource from which the described resource is derived MAbs v. 7, no. 5, 2015. -- p. 820-828 Publisher An entity responsible for making the resource available Taylor & Francis Group Rights Information about rights held in and over the resource <p><strong>Información sobre Derechos de Autor</strong></p> <p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p> <p><strong> La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p> <p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p> Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource Inglés Type The nature or genre of the resource Artículo Identifier An unambiguous reference to the resource within a given context DOI: 10.1080/19420862.2015.1068491 Ensayo Inmuno Enzimatico Inmunoterapia Nanocuerpos