1
25
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https://riquim.fq.edu.uy/files/original/961679e3fdc187b04220a8e6bf337007.pdf
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Dublin Core
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Intranasal delivery of Quillaja brasiliensis saponin-based nanoadjuvants improve humoral immune response of influenza vaccine in aged mice</strong>
Subject
The topic of the resource
QUILLAJA BRASILIENSIS
VACUNAS
NANOTECNOLOGIA
NANOPARTICULAS
MATRICES ISCOM
VIRUS
INFLUENZA
VIA INTRANASAL
RATONES ENVEJECIDOS
BIBLIOGRAFIA NACIONAL QUIMICA
2024
Abstract
A summary of the resource.
Increasing the effectiveness of vaccines against respiratory viruses is particularly relevant for the elderly, since they are prone to develop serious infections due to comorbidities and the senescence of the immune system. The addition of saponin-based adjuvants is an interesting strategy to increase the effectiveness of vaccines. We have previously shown that ISCOM matrices from Q. brasiliensis (IMXQB) are a safe and potent adjuvant. In this study, we evaluated the use of IMXQB as an adjuvant for the seasonal trivalent influenza vaccine (TIV) in an aged mice model. Herein, we show that subcutaneous injection of the adjuvanted vaccine promoted higher titers of IgM, IgG (and isotypes), and serum hemagglutination inhibition titers (HAI). Notably, aged mice immunized by intranasal route also produced higher IgG (and isotypes) and IgA titers up to 120 days after priming, as well as demonstrating an improvement in the HAI antibodies against the TIV. Further, experimental infected aged mice treated once with sera from adult naïve mice previously immunized with TIV-IMXQB subcutaneously successfully controlled the infection. Overall, TIV-IMXQB improved the immunogenicity compared to TIV by enhancing systemic and mucosal immunity in old mice conferring a faster recovery after the H1N1pdm09-like virus challenge. Thus, IMXQB nanoparticles may be a promising platform for next-generation viral vaccines.
Creator
An entity primarily responsible for making the resource
<strong>Silveira, Fernando</strong>
<strong>García, Florencia</strong>
<strong>García, Gabriel</strong>
<strong>Chabalgoity, José A.</strong>
<strong>Rossi, Silvina</strong>
<strong>Baz, Mariana</strong>
Source
A related resource from which the described resource is derived
<span data-sheets-formula-bar-text-style="font-size:13px;color:#000000;font-weight:normal;text-decoration:none;font-family:'Arial';font-style:normal;text-decoration-skip-ink:none;">Vaccines, v. 12, nº 8, 2024. -- e902</span>
Publisher
An entity responsible for making the resource available
MDPI
Date
A point or period of time associated with an event in the lifecycle of the resource
2024
Rights
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<div class="element-text">
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
</div>
Format
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PDF
Extent
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18 p.
Language
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Inglés
Type
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Artículo
Identifier
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<span data-sheets-formula-bar-text-style="font-size:13px;color:#000000;font-weight:normal;text-decoration:none;font-family:'Arial';font-style:normal;text-decoration-skip-ink:none;">10.3390/vaccines12080902</span>
Influenza
Matrices ISCOM
Nanopartículas
Nanotecnología
Quillaja Brasiliensis
Ratones envejecidos
Vacunas
Via intranasal
Virus
-
https://riquim.fq.edu.uy/files/original/461e959364bd022862415077690aec71.pdf
7d655d477419b81ec6c1baf85df26072
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Omeka Image File
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8
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155
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
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Title
A name given to the resource
<strong>A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers</strong>
Subject
The topic of the resource
VIRUS
ENFERMEDADES DE LAS PLANTAS
VIRUS ARN
PROTEINAS
MICROSCOPIA
CRISTALOGRAFIA
RAYOS X
BIBLIOGRAFIA NACIONAL QUIMICA
2023
Abstract
A summary of the resource.
Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein of the mal de Río Cuarto virus (MRCV) are required for its multimerization and the formation of viroplasm like structures. Using an integrative structural approach, the C-arm was found to be dis pensable for P9-1 dimer assembly but essential for the formation of pentamers and hexamers of dimers (decamers and dodecamers), which favored RNA binding. Although both P9-1 and P9-1DC-arm catalyzed ATP with similar activities, an RNA-stimulated ATPase activity was only detected in the full-length protein, indicating a C-arm-mediated interaction between the ATP catalytic site and the allosteric RNA binding sites in the (do)decameric assemblies. A stronger preference to bind phosphate moieties in the dec amer was predicted, suggesting that the allosteric modulation of ATPase activity by RNA is favored in this structural conformation. Our work reveals the structural versatility
Creator
An entity primarily responsible for making the resource
<strong>Llauger, Gabriela</strong>
<strong>Melero, Roberto</strong>
<strong>Monti, Demián</strong>
<strong>Sycz, Gabriela</strong>
<strong>Huck Iriart, Cristián</strong>
<strong>Cerutti, María L.</strong>
<strong>Klinke, Sebastián</strong>
<strong>Mikkelsen, Evelyn</strong>
<a href="https://export.cvuy.uy/cv/?1797974415d19368fea1eae1d35ebba1" target="_blank"><strong>Tijman, Ariel</strong></a>
<strong>Arranz, Rocío</strong>
<strong>Alfonso, Victoria</strong>
<strong>Arellano, Sofía M.</strong>
<strong>Goldbaum, Fernando A.</strong>
<strong>Sterckx, Yann G. J.</strong>
<strong>Carazo, José María</strong>
<strong>Kaufman, Sergio B.</strong>
<strong>Dans, Pablo D.</strong>
<strong>del Vas, Mariana</strong>
<strong>Otero, Lisandro H.</strong>
Source
A related resource from which the described resource is derived
MBio, v.14, n°2, 2023
Publisher
An entity responsible for making the resource available
American Society for Microbiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2023
Rights
Information about rights held in and over the resource
<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Extent
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24 p.
Language
A language of the resource
Inglés
Type
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Artículo
Identifier
An unambiguous reference to the resource within a given context
10.1128/mbio.00023-23
Cristalografía
Enfermedades de las plantas
Microscopía
Proteínas
Rayos X
Virus
Virus ARN
-
https://riquim.fq.edu.uy/files/original/169c842b9f72874f55d6b64c428df27e.pdf
26eae7e0c2a58a43c61fe0694186c6ec
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
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Dublin Core
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
PDF
Dublin Core
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Title
A name given to the resource
<strong>Sortase-Mediated Phage Decoration for Analytical Applications</strong>
Subject
The topic of the resource
INMUNOLOGIA
VIRUS
PEPTIDOS
PROTEINAS
ETIQUETADO
BIOPOLIMEROS
BIBLIOGRAFIA NACIONAL QUIMICA
2021
Abstract
A summary of the resource.
<p>Phage-borne peptides and antibody fragments isolated from phage display libraries have proven to be versatile and<br />valuable reagents for immunoassay development. Due to the lack of convenient and mild-condition methods for the labeling of the phage particles, isolated peptide/protein affinity ligands are commonly removed from the viral particles and conjugated to protein tracers or nanoparticles for analytical use. This abolishes the advantage of isolating ready-to-use affinity binders and creates the risk of affecting the polypeptide activity. To circumvent this problem, we optimized the phage display system to produce phage particles that express the affinity binder on pIII and a polyglycine short peptide fused to pVIII that allows the covalent attachment of tracer molecules employing sortase A. Using a llama heavy chain only variable domain (VHH) against the herbicide 2,4-D on pIII as the<br />model, we showed that the phage can be extensively decorated with a rhodamine-LPETGG peptide conjugate or the protein<br />nanoluciferase (Nluc) equipped with a C-terminal LPETGG peptide. The maximum labeling amounts of rhodamine-LPETGG and Nluc-LPETGG were 1238 ± 63 and 102 ± 16 per phage, respectively. The Nluc-labeled dual display phage was employed to develop a phage bioluminescent immunoassay (P-BLEIA) for the detection of 2,4-D. The limit of detection and 50% inhibition<br />concentration of P-BLEIA were 0.491 and 2.15 ng mL−1, respectively, which represent 16-fold and 8-fold improvement compared to the phage enzyme-linked immunosorbent assay. In addition, the P-BLEIA showed good accuracy for the detection of 2,4-D in spiked samples.</p>
Creator
An entity primarily responsible for making the resource
<strong>Ding, Yuan</strong>
<strong>Chen, He</strong>
<strong>Li, Jiao</strong>
<strong>Huang, Lianrun</strong>
<strong>Song, Guangyue</strong>
<strong>Li, Zhenfeng</strong>
<strong>Hua, Xiude</strong>
<a href="https://exportcvuy.anii.org.uy/cv/?f843e2759f6ee96f8a16e51ceccfaeb41eb83154ba5da266ce35c9e64d7ad49b7ee8622e336b48ac9846dbe51b76b49fb1d2738fdaf473a4c75dea6e4f1afd20" target="_blank"><strong>González Sapienza, Gualberto</strong></a>
<strong>Hammock, Bruce D.</strong>
<strong>Wang, Minghua</strong>
Source
A related resource from which the described resource is derived
Analytical Chemistry, v. 93, n° 34, 2021 .-- pp. 11800-11808.
Publisher
An entity responsible for making the resource available
American Chemical Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
Rights
Information about rights held in and over the resource
<p dir="ltr"><strong>Información sobre Derechos de Autor</strong></p>
<p dir="ltr"><span>(Por favor lea este aviso antes de abrir los documentos u objetos)</span></p>
<p dir="ltr"><strong>La legislación uruguaya protege el derechode autor</strong><span> sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</span></p>
<span id="docs-internal-guid-9d2103f7-7fff-c813-00c7-2528fd4891ff"><strong>ADVERTENCIA:</strong><span> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</span></span>
Format
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PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Artículo
Identifier
An unambiguous reference to the resource within a given context
<p align="JUSTIFY"><span style="font-family: Arial, sans-serif;"><span style="font-size: small;"><span style="color: #000000;"><span><span><span>10.1021/acs.analchem.1c02322</span></span></span></span></span></span></p>
Biopolímeros
Etiquetado
Inmunología
Péptidos
Proteínas
Virus
-
https://riquim.fq.edu.uy/files/original/434f5f4f5b307f7d70ee0da73e802050.pdf
423249b0e3aeb9313910e8bb298d183e
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Omeka Image File
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8
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3
Height
153
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135
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Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
If the image is of an object, state the type of object, such as painting, sculpture, paper, photo, and additional data
<p>PDF</p>
<p> </p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>The Distribution of Onion Virulence Gene Clusters Among Pantoea spp.</strong>
Subject
The topic of the resource
CEBOLLAS
BACTERIAS
VIRUS
ENFERMEDADES DE LAS PLANTAS
BIBLIOGRAFIA NACIONAL QUIMICA
2021
Abstract
A summary of the resource.
<p>Pantoea ananatis is a gram-negative bacterium and the primary causal agent of center rot of onions in Georgia. Previous genomic studies identified two virulence gene clusters, HiVir and alt, associated with center rot. The HiVir gene cluster is required to induce necrosis on onion tissues via synthesis of pantaphos, (2-hydroxy[phosphonomethyl) maleate), a phosphonate phytotoxin. The alt gene cluster aids in tolerance<br />to thiosulfinates generated during onion tissue damage. Whole genome sequencing of other Pantoea species suggests that these gene clusters are present outside of P. ananatis. To assess the distribution of these gene clusters, two PCR primer sets were designed to detect the presence of HiVir and alt. Two hundred fifty-two strains of Pantoea spp. were phenotyped using the red onion scale necrosis (RSN) assay and were genotyped using PCR for the presence of these virulence genes. A diverse panel of strains from three distinct culture collections comprised of 24 Pantoea species, 41 isolation sources, and 23 countries, collected from 1946–2019, was tested. There is a<br />significant association between the alt PCR assay and Pantoea strains recovered from symptomatic onion (P < 0.001). There is also a significant association of a positive HiVir PCR and RSN assay among P. ananatis strains but not among Pantoea spp.,<br />congeners. This may indicate a divergent HiVir cluster or different pathogenicity and virulence mechanisms. Last, we describe natural alt positive [RSN C /HiVir C /alt C ]<br />P. ananatis strains, which cause extensive bulb necrosis in a neck-to-bulb infection assay compared to alt negative [RSN C /HiVir C /alt</p>
Creator
An entity primarily responsible for making the resource
<strong>Stice, Shaun P.</strong>
<strong>Shin, Gi Yoon</strong>
<strong>De Armas, Stefanie</strong>
<strong>Koirala, Santosh</strong>
<strong>Galván, Guillermo A.</strong>
<a href="https://exportcvuy.anii.org.uy/cv/?cfed0661e796b3b7a6c89a05745b635f6e13ddb8d5177c67cc3b1bee6429641382fbd641b8d389c25effb4a604816b6183886da64c1bb3218a0e70430beb6c69" target="_blank"><strong>Siri, María Inés</strong></a>
<strong>Severns, Paul M.</strong>
<strong>Coutinho, Teresa</strong>
<strong>Dutta, Bhabesh</strong>
<strong>Kvitko, Brian H.</strong>
Source
A related resource from which the described resource is derived
Frontiers in Plant Science v. 12. 2021. -- e643787
Publisher
An entity responsible for making the resource available
Frontiers
Date Available
Date (often a range) that the resource became or will become available.
2021
Rights
Information about rights held in and over the resource
<strong>Información sobre Derechos de Autor</strong>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho de autor</strong> sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA</strong> - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
An unambiguous reference to the resource within a given context
<a href="https://doi.org/10.3389/fpls.2021.643787">https://doi.org/10.3389/fpls.2021.643787</a>
Bacterias
Cebollas
Enfermedades de las plantas
Virus
-
https://riquim.fq.edu.uy/files/original/806e50031011d9ebdd05c12d72e25389.jpg
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Omeka Image File
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Bit Depth
8
Channels
3
Height
84
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146
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Original Format
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Papel
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Desinfección del alcohol etílico al 70% sobre los siguientes virus : HIV, hepatitis B y C</strong>
Subject
The topic of the resource
<strong>ALCOHOL ETILICO</strong>
<strong>DESINFECCION</strong>
<strong>VIRUS</strong>
<strong>ENFERMEDADES PRODUCIDAS POR VIRUS</strong>
<strong>VIRUS DE INMUNODEFICIENCIA HUMANA</strong>
<strong>HEPATITIS VIRICA</strong>
Publisher
An entity responsible for making the resource available
Biblioteca-FQ
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
Format
The file format, physical medium, or dimensions of the resource
Papel
PDF
Language
A language of the resource
Inglés
Type
The nature or genre of the resource
Bibliografía
Temporal Coverage
Temporal characteristics of the resource.
1984-1998
Alcohol etílico
Desinfección
Enfermedades producidas por virus
Hepatitis vírica
Virus
Virus de inmunodeficiencia humana
-
https://riquim.fq.edu.uy/files/original/f762d1502f0b8198f4a30079fafd42c6.pdf
0ebebc2e46cd599f97051dd7aefd7031
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Omeka Image File
The metadata element set that was included in the `files_images` table in previous versions of Omeka. These elements are common to all image files.
Bit Depth
8
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3
Height
154
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120
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
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Departamento de Documentación y Biblioteca
RIQUIM
Document
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PDF
Dublin Core
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Title
A name given to the resource
<strong>Evaluation of a Labelled Bacteriophage with 99mTc as a Potential Agent for Infection Diagnosis</strong>
Subject
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VIRUS
RADIOQUIMICA
BACTERIOFAGO
DIAGNOSTICO
BIBLIOGRAFIA NACIONAL QUIMICA
2016
Abstract
A summary of the resource.
The design of target-specific molecular imaging probes to determine infection sites are mainly based on the biochemistry of the inflammatory response that may lead to an ideal agent for infection imaging. Infectious diseases timely and specifically diagnosed can be clinically challenging but essential for the patient's recovery. Laboratory tests can detect the responsible microorganism but cannot discriminate between sterile inflammatory disease and truly infectious disease.
Creator
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<strong>Cardoso, María Elena</strong>
<strong>Fernández, Leticia</strong>
<strong>Tejería, Emilia</strong>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8b87e8e50f3fa6cbb61ecc6edf70e6f8" target="_blank"><strong>Esperón, Patricia</strong></a>
<a title="Curriculum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=5d09a7215277d361b69aefbb081c9fa8" target="_blank"><strong>Terán, Mariella</strong></a>
Source
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Current Radiopharmaceuticals v. 9, no. 2, 2016. -- p. 137-142
Publisher
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Bentham Science
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes</p>
Format
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PDF
Language
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Inglés
Type
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Artículo
Identifier
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DOI: 10.2174/1874471008666151027154148
Bacteriófagos
Diagnóstico
Radioqimica
Virus
-
https://riquim.fq.edu.uy/files/original/521797ea709ccbaccccef0b24a636b56.pdf
08ca3bc617b3bad9c851a8f6c38d1387
https://riquim.fq.edu.uy/files/original/7a07daf302f5c9388faa5fbe13b2b279.JPG
5737bb9201391384660f25a5d20b56b1
Omeka Image File
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Bit Depth
8
Channels
3
Height
199
Width
185
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bibliografía Nacional Química
Description
An account of the resource
Contiene la producción científica nacional de los profesionales químicos e investigadores, durante el período 1929 hasta la fecha.
Contributor
An entity responsible for making contributions to the resource
Departamento de Documentación y Biblioteca
RIQUIM
Document
A resource containing textual data. Note that facsimiles or images of texts are still of the genre text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
<strong>Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant</strong>
Subject
The topic of the resource
INMOVILIZACIOÓN DE ENZIMAS
HISTIDINA
VIRUS
BIBLIOGRAFIA NACIONAL QUÍMICA
2009
Abstract
A summary of the resource.
Addition of tags [such as His (histidine) tags] is extremely helpful for the affinity purification of recombinant proteins. In several cases, these tags must be removed before performing functional and structural studies. The enzyme most frequently used to cleave tags of recombinant proteins is the TEV-protease (tobacco-etch-virus NIa protease). The continuous production of this enzyme in soluble form is quite an expensive process and not easily accessible to many laboratories. Thus an interesting alternative is the use of TEV-protease in an immobilized form, which may be reutilized several times. The main objective of the present study was to obtain a TEV-protease in an immobilized form, by covalent immobilization on to solid supports through selective use of different amino acid residues, lysine or cysteine. High protein immobilization yields (75–97 %) were obtained with both strategies. The TEV-protease immobilized through its exposed cysteine thiol groups maintained its ability for cleaving a 20 kDa substrate. While the activity of the immobilized TEV-protease maintained only 30% of the activity of the enzyme in soluble form, its stability at 4 ◦C was improved three times. Moreover, this enzyme could be reutilized in at least five cycles of cleavage without loss of performance. The present results indicate that the use of a TEV-protease in an immobilized form is a potentially useful tool for the cleavage of His tags of recombinant proteins and may be useful for reducing the cost of the total process
Creator
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<strong>Puhl, Ana Cristina</strong>
<a title="Currículum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=3c8a423235a59fcbc901eec52b2b7794" target="_blank"><strong>Giacomini, Cecilia</strong></a>
<a title="Currículum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=da4f071f890fec490234707830d5f695" target="_blank"><strong>Irazoqui, Gabriela</strong></a>
<a title="Currículum Vitae" href="http://buscadores.anii.org.uy/buscador_sni/exportador/ExportarPdf?hash=8dfa5283acaafc5c73dab5b61b04f9a0" target="_blank"><strong>Batista-Viera, Francisco</strong></a>
<strong>Villarino, Andrea</strong>
<strong>Terenzi, Hernan</strong>
Source
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Biotechnology and Applied Biochemistry v. 53, no. 3, 2009. -- p.165-174.
Publisher
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Wiley-Blackwell.
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
Rights
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<p><strong>Información sobre Derechos de Autor</strong></p>
<p>(Por favor lea este aviso antes de abrir los documentos u objetos)</p>
<p><strong>La legislación uruguaya protege el derecho</strong> de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)</p>
<p><strong>ADVERTENCIA -</strong> La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.</p>
Format
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PDF
Language
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Ingles
Type
The nature or genre of the resource
Artículo
Identifier
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DOI:10.1042/BA20080063
Histidina
Inmovilización de enzimas
Virus