Molecular marker diversity and bacterial wilt resistance in wild Solanum commersonii accessions from Uruguay

Dublin Core

Título

Molecular marker diversity and bacterial wilt resistance in wild Solanum commersonii accessions from Uruguay

Tema

SOLANUM COMMERSONII
BIBLIOGRAFIA NACIONAL QUIMICA
URUGUAY
2009

Abstract

Solanum commersonii is a wild tuber-bearing species native to Uruguay with high potential for use in potato breeding programs. Little is known about the genetic diversity within this wild species and the relationship with the resistance to the bacterial pathogen Ralstonia solanacearum. We studied 30 S. commersonii clonal accessions, 20 of which were collected from geographically diVerent areas across the country, while the other ten were grown from seeds from a single plant. Resistance against R. solanacearum was tested and diVerent levels of resistance were found, ranging from delayed wilting to asymptomatic reactions. The genetic variation and the relationships among individuals in this germplasm collection were studied by diVerent molecular markers: Random AmpliWed Polymorphic DNA (RAPD), AmpliWed Fragment Length Polymorphism (AFLP) and Microsatellites or Simple Sequence Repeats (SSR). AFLP markers generated the largest number of total and polymorphic fragments per assay unit while SSR revealed the highest frequency of polymorphic bands (100%), followed by AFLP (96.2%) and RAPD (89.4%). In contrast, when comparing the number of diVerent genetic proWles generated, the SSR markers exhibited the lowest discriminatory power. The clustering pattern obtained with the three marker systems showed a similar distribution of the S. commersonii germplasm revealing a high correlation between the three methods employed. All three dendrograms grouped most of the accessions into two main clusters, containing the same accessions regardless of the marker type. Bacterial wilt resistant accessions were present in both clusters. Accessions originated from diVerent seeds of the same plant were grouped within one of the major clusters, and diVered in the response to R. solanacearum revealing segregation of resistance. Furthermore, the distribution in two main clusters showed high correspondence with the geographical origin of the accessions, from the north and south of the country, and with the subspecies malmeanum and commersonii morphologically identified

Autor

Galvan, G.A.
Quirici, L.
Silvera, E.
Villanueva, P.

Fuente

Euphytica  v. 165, no. 2, 2009. -- p.371-382

Editor

Springer

Fecha

2009

Derechos

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Formato

PDF

Idioma

Ingles

Tipo

Artículo

Identificador

DOI 10.1007/s10681-008-9800-8

Cobertura

Uruguay
Fecha de agregación
June 5, 2014
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
Citación
Siri, M. I., “Molecular marker diversity and bacterial wilt resistance in wild Solanum commersonii accessions from Uruguay,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed July 18, 2019, http://riquim.fq.edu.uy/items/show/1522.
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