Assessment of HV1 and HV2 mtDNA variation for forensic purposes in an Uruguayan population sample.

URUGUAY

POBLACION

ESTUDIOS FORENSES

BIBLIOGRAFIA NACIONAL QUIMICA

2005

In order to assess the utility of mtDNA typing for forensic purposes in Uruguay, a population sample of 120 maternally unrelated individuals were amplified and directly sequenced for the HV1 and for the HV2 segments in the control region of the human mtDNA, following previous international recommendations (1). Blood and saliva samples were taken from 120 unrelated individuals and deposited into FTA cards (FTA card, Whatman WB 12-0205). The DNA was extracted in a 1.2 mm punch according to the manufacturer recommendations. DNA amplification and sequencing was performed according to previously published paper (2). All statistical analysis were calculated using the Arlequin Software ver. 2.000 (3). Results are shown in Table 1. In this population sample, 107 different mtDNA sequences were observed out of 120 individuals. Of these, 97 sequences were observed once (80.8%), 8 sequences were observed twice (13.3%), one sequence was observed three times (2.5%), and another four times (3.3%). The variation of mtDNA HV1 and HV2 regions was confined to 146 positions, of which 99 was observed in the HV1 and 47 in the HV2. Sequence variation consisted of 120 transitions, 21 transversions, and 11 indels. The genetic diversity value for combined HV1 and HV2 regions was 0.998 (±0.002) in this Uruguayan population sample, and the nucleotide diversity was 0.068 (±0.035). The complete dataset is available to any interested researcher at: http://www.lorgen.com/forense

Journal of Forensic Sciences v. 50, no. 5, 2005. -- p. 1239-1242

ASTM

2005

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Inglés

Artículo

ID JFS2004362

Uruguay

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