Development of a Simple Method for the Determination of Toxicologically Relevant Species of Arsenic in Urine Using HG-AAS

TOXICOLOGIA

ARSENICO

ORINA

ANALISIS CLINICOS

BIBLIOGRAFIA NACIONAL QUIMICA

2015

Humans are exposed to arsenic (As) in the environment, in both organic and inorganic forms, and it has been widely demonstrated that the inorganic arsenic species (arsenate and arsenite) are the main toxic ones being drinking water one of the main sources of exposure worldwide. Urinary arsenic level is the recommended biomarker for assessing human exposure risks. Absorbed inorganic arsenic is metabolized to mono- and dimethylated arsenic compounds (MMA and DMA) prior to its excretion in urine. In this work, a simple procedure for the determination of toxicologically relevant arsenic species in urine (sum of As(III), As(V), MMA and DMA) using Hydride Generation Atomic Absorption Spectrometry (HG-AAS) was developed in order to obtain a feasible and self-sustainable technology for analysis and epidemiological studies when other expensive techniques are not available. Sample preparation was based on the derivation of arsenic species with L-cysteine. The limit of detection was 1.5 μg L-1, linearity up to 50 μg L-1 and analytical precision was 12 % (relative standard deviation, RSD %, n=10). Trueness was evaluated using spiked urine samples as well as a reference material. The range of acceptation for recoveries was established in 85-115% by means of a t-test at the 95% significance level. Recoveries for the four As species from spiked urine samples were in the range 87.8-113.5 %. The recovery from a reference material was 100.7%. The comparison between the HG-AAS and high performance liquid chromatography-hydride generation-coupled to inductively coupled plasma mass spectrometry (HPLC-HG-ICPMS), considered as a gold standard technique, showed good agreement (R2 = 0.94) for forty urine samples. The figures of merit were adequate for the determination of relevant species of As for biomonitoring purposes according to international regulations and it can be postulated as an alternative to more expensive techniques

Journal of Environment Pollution and Human Health v. 3, 2015. -- p. 46-51

Science and Education Publishing

2015

Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes

(LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

PDF

Inglés

Artículo

DOI:10.12691/jephh-3-2-4

PDF