Natural and induced antibodies contribute to differentialsusceptibility to secondary cystic echinococcosis of Balb/c andC57Bl/6 mice

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Título

Natural and induced antibodies contribute to differentialsusceptibility to secondary cystic echinococcosis of Balb/c andC57Bl/6 mice

Tema

ECHINOCOCCUS GRANULOSUS
INMUNOBIOLOGIA
ANTICUERPOS
BIBLIOGRAFIA NACIONAL QUIMICA
2016

Abstract

tAntibodies are key immune players in several helminth infections and animal models have been centralfor the identification of their mechanisms of protection. Murine secondary cystic echinococcosis is auseful model for studying Echinococcus granulosus immunobiology, being the immune profile mountedby the experimental host a determinant of parasite success or failure in infection establishment. In thepresent study, we analyzed infection outcome using Balb/c and C57Bl/6 mice strains, and compared theirantibody responses in terms of quality and intensity. Our results showed that Balb/c is a highly susceptiblestrain to secondary cystic echinococcosis, while C57Bl/6 mice are quite resistant. Moreover, significantdifferences between strains were observed in natural and induced antibodies recognizing E. granulosusantigens, both at the systemic and peritoneal levels. Natural cross-reacting IgM, IgG2b and IgG3 antibodieswere detected in sera from both strains but with different intensities, and – remarkably – natural IgG2bshowed to be an intrinsic correlate of protection in both mice strains. Interestingly, naïve C57Bl/6 serumdisplayed a higher protoscolicidal activity, and heterologous – but not homologous – transference ofC57Bl/6 naïve serum into Balb/c mice, significantly reduced their infection susceptibility. In the peritonealcavity, different levels of natural cross-reacting IgM and IgG3 antibodies were detected in both micestrains, while cross-reacting IgG2b was detected only in C57Bl/6 mice. On the other hand, infected micefrom both strains developed isotype-mixed antibody responses, with Balb/c mice biasing their responsetowards high avidity IgG1 and C57Bl/6 mice showing a predominance of mixed IgM/IgG2c/IgG2b/IgG3.In this regard, IgG1 levels showed to be a correlate of susceptibility in both mice strains. In conclusion,our results suggest that antibodies – either natural or induced – play a role in the susceptibility degreeto murine secondary cystic echinococcosis.

Autor

Mourglia Ettlin, Gustavo Daniel
Cucher, Marcela
Arbildi, Paula
Rosenzvit, Mara

Fuente

Immunobiology v. 221, 2016. -- p. 103-115

Editor

Elsevier

Fecha

2016

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PDF

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Tipo

Artículo

Identificador

http://dx.doi.org/10.1016/j.imbio.2015.07.016

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Original Format

PDF
Fecha de agregación
May 12, 2016
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
Mourglia Ettlin, Gustavo Daniel, “Natural and induced antibodies contribute to differentialsusceptibility to secondary cystic echinococcosis of Balb/c andC57Bl/6 mice,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed October 20, 2020, http://riquim.fq.edu.uy/items/show/4003.
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