Targeted and untargeted high resolution mass approach for a putative profiling of glycosylated simple phenols in hybrid grapes

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Título

Targeted and untargeted high resolution mass approach for a putative profiling of glycosylated simple phenols in hybrid grapes

Tema

CROMATOGRAFIA LIQUIDA
FENOLES GLICOSILADOS
UVAS HIBRIDAS
BIBLIOGRAFIA NACIONAL QUIMICA
2017

Abstract

Vitis vinifera is one of the most widespread grapevines around the world representing the raw material for high quality wine production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generated much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids have not been well defined, particularly for the simple phenols profile. The dynamic of these phenols in wines, where the glycosylated forms can be transformed into the free ones during winemaking, also raises an increasing health interest by their role as antoxidants in wine consumers. In this work an on-line SPE clean-up device, to reduce matrix interference, was combined with ultra-high liquid chromatography-high resolution mass spectrometry in order to increase understanding of the phenolic composition of hybrid grape varieties. Specifically, the phenolic composition of 4 hybrid grape varieties (red, Cabernet Cantor and Prior; white, Muscaris and Solaris) and 2 European grape varieties (red, Merlot; white, Chardonnay) was investigated, focusing on free and glycosidically bound simple phenols and considering compound distribution in pulp, skin, seeds and wine. Using a targeted approach 53 free simple phenols and 7 glycosidic precursors were quantified with quantification limits ranging from 0.001 to 2 mg Kg− 1 and calibration R2 of 0.99 for over 86% of compounds. The untargeted approach made it possible to tentatively identify 79 glycosylated precursors of selected free simple phenols in the form of -hexoside (N = 30), -pentoside (21), -hexoside-hexoside (17), -hexoside-pentoside (4), -pentoside-hexoside (5) and -pentoside-pentoside (2) derivatives on the basis of accurate mass, isotopic pattern and MS/MS fragmentation.

Autor

Barnaba, Chiara
Nicolini, Giorgio
Giacomelli, Mattia
Villegas, Tomas Roman
Nardin, Tiziana
Larcher, Roger

Fuente

Food Research International v. 98, 2017. -- p. 20-33

Editor

Elsevier

Fecha

2017

Derechos

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Formato

PDF

Idioma

Inglès

Tipo

Artículo

Identificador

DOI: 10.1016/j.foodres.2017.01.011

Document Item Type Metadata

Original Format

PDF
Fecha de agregación
January 10, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
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Citación
Barnaba, Chiara, “Targeted and untargeted high resolution mass approach for a putative profiling of glycosylated simple phenols in hybrid grapes,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed October 17, 2018, http://riquim.fq.edu.uy/items/show/4821.
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