A novel thermophilic and halophilic esterase from Janibacter sp. R02,the first member of a new lipase family (Family XVII)

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Título

A novel thermophilic and halophilic esterase from Janibacter sp. R02,the first member of a new lipase family (Family XVII)

Tema

LIPASAS
JANIBACTER
BIBLIOGRAFIA NACIONAL QUIMICA
2017

Abstract

Janibacter sp. strain R02 (BNM 560) was isolated in our laboratory from an Antarctic soil sample. A remarkable trait of the strain was its high lipolytic activity, detected in Rhodamine-olive oil supplemented plates. Supernatants of Janibacter sp. R02 displayed superb activity on transesterification of acyl glycerols, thus being a good candidate for lipase prospection. Considering the lack of information concerning lipases of the genus Janibacter, we focused on the identification, cloning, expression and characterization of the extracellular lipases of this strain. By means of sequence alignment and clustering of consensus nucleotide sequences, a DNA fragment of 1272 bp was amplified, cloned and expressed in E. coli. The resulting recombinant enzyme, named LipJ2, showed preference for short to medium chain-length substrates, and displayed maximum activity at 80 °C and pH 8–9, being strongly activated by a mixture of Na+ and K+. The enzyme presented an outstanding stability regarding both pH and temperature. Bioinformatics analysis of the amino acid sequence of LipJ2 revealed the presence of a consensus catalytic triad and a canonical pentapeptide. However, two additional rare motifs were found in LipJ2: an SXXL β-lactamase motif and two putative Y-type oxyanion holes (YAP). Although some of the previous features could allow assigning LipJ2 to the bacterial lipase families VIII or X, the phylogenetic analysis showed that LipJ2 clusters apart from other members of known lipase families, indicating that the newly isolated Janibacter esterase LipJ2 would be the first characterized member of a new family of bacterial lipases.

Autor

Castilla, Agustín
Panizza, Paola
Rodríguez, Diego
Bonino, Luis
Díaz, Pilar
Rodríguez Giordano, Sonia

Fuente

Enzyme and Microbial Technology v. 98, 2017. -- p. 86-95

Editor

Elsevier

Fecha

2017

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PDF

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Tipo

Artículo

Identificador

DOI: 10.1016/j.enzmictec.2016.12.010

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Original Format

PDF
Fecha de agregación
January 15, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
,
Citación
Castilla, Agustín, “A novel thermophilic and halophilic esterase from Janibacter sp. R02,the first member of a new lipase family (Family XVII),” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed February 25, 2018, http://riquim.fq.edu.uy/items/show/4827.
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