In Vitro Anti/Pro-oxidant Activities of R. ferruginea Extract and Its Effect on Glioma Cell Viability : Correlation with Phenolic Compound Content and Effects on Membrane Dynamics

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Título

In Vitro Anti/Pro-oxidant Activities of R. ferruginea Extract and Its Effect on Glioma Cell Viability : Correlation with Phenolic Compound Content and Effects on Membrane Dynamics

Tema

LIPOSOMAS
COMPUESTOS FENOLICOS
GLIOMA
BIBLIOGRAFIA NACIONAL QUIMICA
2018

Abstract

Rapanea ferruginea antioxidant and antitumoral properties were not explored before in literature. This study aimed to investigate these biological activities for the R. ferruginea leaf extract and correlate them with its phenolic content and influence in biological membrane dynamics. Thus, in this study, anti/pro-oxidative properties of R. ferruginea leaf extract by in vitro DPPH and TBARS assays, with respect to the free radical reducing potential and to its activity regarding membrane free radical-induced peroxidation, respectively. Furthermore, preliminary tests related to the extract effect on in vitro glioma cell viability were also performed. In parallel, the phenolic content was detected by HPLC-DAD and included syringic and trans-cinnamic acids, quercetrin, catechin, quercetin, and gallic acid. In an attempt to correlate the biological activity of R. ferruginea extract and its effect on membrane dynamics, the molecular interaction between the extract and a liposomal model with natural-sourced phospholipids was investigated. Location and changes in vibrational, rotational, and translational lipid motions, as well as in the phase state of liposomes, induced by R. ferruginea extract, were monitored by Fourier-transform infrared spectroscopy, nuclear magnetic resonance, differential scanning calorimetry, and UV-visible spectroscopy. In its free form, the extract showed promising in vitro antioxidant properties. Free-form extract (at 1000µ g/mL) exposure reduced glioma cell in vitro viability in 40%, as evidenced by MTT tests. Pro-oxidant behavior was observed when the extract was loaded into liposomes. A 70.8% cell viability reduction was achieved with 500 µg/mL of liposome-loaded extract. The compounds of R. ferruginea extract ordered liposome interface and disorder edits a polar region. Phenolic content, as well as membrane interaction and modulation may have an important role in the oxidative and antitumoral activities of the R. ferruginea leaf extract.

Autor

Magalhães dos Santos, Desirée
Jardim Rocha, Camila Valesca
Ferreira da Silveira, Elita
Germani Marinho, Marcelo Augusto
Osti Silva, Nichole
da Silva Ferreira, Ailton
Fernandes de Moura, Neusa
Sagrera Darelli, Gabriel Jorge.
Braganhol, Elizandra
Horn, Ana Paula
Rodrigues de Lima, Vânia

Fuente

The Journal of Membrane Biology v. 251, 2018. --p. 247-261

Editor

Springer

Fecha

2018

Derechos

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

DOI:10.1007/s00232-018-0017-z

Document Item Type Metadata

Original Format

PDF
Fecha de agregación
August 29, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
Magalhães dos Santos, Desirée, “In Vitro Anti/Pro-oxidant Activities of R. ferruginea Extract and Its Effect on Glioma Cell Viability : Correlation with Phenolic Compound Content and Effects on Membrane Dynamics,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed February 18, 2020, http://riquim.fq.edu.uy/items/show/5102.
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