Leaf saponins of Quillaja brasiliensis enhance long-term specific immune responses and promote dose-sparing effect in BVDV experimental vaccines

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Título

Leaf saponins of Quillaja brasiliensis enhance long-term specific immune responses and promote dose-sparing effect in BVDV experimental vaccines

Tema

SAPONINAS
INMUNOLOGIA
QUILLAJA BRASILIENSIS
VACUNAS
BIBLIOGRAFIA NACIONAL QUIMICA
2018

Abstract

Saponin-based adjuvants are promising adjuvants that enhance both humoral and T-cell-mediated immunity. One of the most used natural products as vaccine adjuvants are Quillaja saponaria bark saponins and its fraction named Quil A®. Despite that, its use has been restricted for human use due to safety issues. As an alternative, our group has been studying the congener species Quillaja brasiliensis saponins and its performance as vaccine adjuvants, which have shown to trigger humoral and cellular immune responses comparable to Quil A® but with milder side effects. Here, we studied a semi purified aqueous extract (AE) and a previously little characterized saponin-enriched fraction (QB-80) from Q. brasiliensis as vaccine adjuvants and an inactivated virus (bovine viral diarrhea virus, BVDV) antigen co-formulated in experimental vaccines in mice model. For the first time, we show the spectra pattern of the Q. brasiliensis saponins by MALDI-TOF, a novel and cost-effective method that could be used to characterize different batches during saponins production. Both AE and QB-80 exhibited noteworthy chemical similarities to Quil A®. In addition, the haemolytic activity and toxicity were assessed, showing that both AE and QB-80 were less toxic than Quil A®. When subcutaneously inoculated in mice, both fractions promoted long-term strong antibody responses encompassing specific IgG1 and IgG2a, enhanced the avidity of IgG antibodies, induced a robust DTH reaction and significantly increased IFN-ɣ production in T CD4+ and T CD8+ cells. Furthermore, we have proven herein that AE has the potential to promote dose-sparing, substantially reducing the dose of antigen required for the BVDV vaccines and still eliciting a mixed Th1/Th2 strong immune response. Based on these results, and considering that AE is a raw extract, easier and cheaper to produce than commercially available saponins, this product can be considered as candidate to be escalated from experimental to industrial uses.

Autor

Cibulski, Samuel
Rivera Patrón, Mariana.
Suárez, Norma
Pirez, Macarena
Rossi, Silvina
Yendo, Anna Carolina
de Costa, Fernanda
Gosmann, Grace
Fett Neto, Arthur
Roehe, Paulo Michel
Silveira, Fernando

Fuente

Vaccine  v. 36, 2018. -- p. 55-65

Editor

Elsevier

Fecha

2018

Derechos

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PDF

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Tipo

Artículo

Identificador

DOI: 10.1016/j.vaccine.2017.11.030

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Original Format

PDF
Fecha de agregación
November 26, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
Cibulski, Samuel, “Leaf saponins of Quillaja brasiliensis enhance long-term specific immune responses and promote dose-sparing effect in BVDV experimental vaccines,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed May 28, 2020, http://riquim.fq.edu.uy/items/show/5444.
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