Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers

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Título

Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers

Tema

EQUINOCOCOSIS QUISTICA
BIOMARCADORES
SEROLOGIA
DIAGNOSTICO
ESTADIFICACION DEL QUISTE
BIBLIOGRAFIA NACIONAL QUIMICA
2020

Abstract

The reference diagnostic method of human abdominal Cystic Echinococcosis (CE) is imaging, particularly ultrasound, supported by serology when imaging is inconclusive. However, current diagnostic tools are neither optimal nor widely available. The availability of a test detecting circulating biomarkers would considerably improve CE diagnosis and cyst staging (active vs inactive), as well as treatments and follow-up of patients. Exosomes are extracellular vesicles involved in intercellular communication, including immune system responses, and are a recognized source of biomarkers. With the aim of identifying potential biomarkers, plasma pools from patients infected by active or inactive CE, as well as from control subjects, were processed to isolate exosomes for proteomic label-free quantitative analysis. Results were statistically processed and subjected to bioinformatics analysis to define distinct features associated with parasite viability. First, a few parasite proteins were identified that were specifically associated with either active or inactive CE, which represent potential biomarkers to be validated in further studies. Second, numerous identified proteins of human origin were common to active and inactive CE, confirming an overlap of several immune response pathways. However, a subset of human proteins specific to either active or inactive CE, and central in the respective protein-protein interaction networks, were identified. These include the Src family kinases Src and Lyn, and the immune-suppressive cytokine TGF-β in active CE, and Cdc42 in inactive CE. The Src and Lyn Kinases were

Autor

Fratini, Federica
Tamarozzi, F.
Macchia, G.
Bertuccini, L.
Mariconti, M.
Birago, C.
Iriarte, A.
Brunetti, E.
Cretu, C. M.
Akhan, O.
Siles-Lucas, M.
Díaz, A.
Casulli, Adriano

Fuente

Plos Neglected Tropical Diseases v.14, no. 10, 2020.-- p. 1-31. -- e0008586

Editor

Public Library of Science

Fecha

2020

Derechos

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Inglés

Tipo

Artículo

Identificador

https://doi.org/10.1371/journal. pntd.0008586

Document Item Type Metadata

Original Format

Pdf
Fecha de agregación
March 1, 2021
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
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Citación
Fratini, Federica, “Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed January 20, 2022, http://riquim.fq.edu.uy/items/show/6192.
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