Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA

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Título

Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA

Tema

ESPECTROSCOPIA DE MASAS
PECES
TEJIDOS (BIOLOGIA)
ANALISIS QUIMICO
CIANOTOXINAS
BIBLIOGRAFIA NACIONAL QUIMICA
2011

Abstract

Microcystins (MCs) are the most common cyanotoxins found worldwide in freshwater, brackish, and marine environments. The rapid and accurate analysis of MCs and nodularin (Nod-R) in fish tissue is important for determining occurrence, following trends, and monitoring exposure for risk assessment and other purposes. The aim of this study was to develop a streamlined and reliable sample preparation method for eight MCs (MC-RR, MCYR, MC-LR, MC-WR, MC-LA, MC-LY, MC-LW, and MCLF) and Nod-R in fish, and conduct a validation of the new method using liquid chromatography–tandem mass spectrometry (LC-MS/MS) for analysis and compare the results with a commercial enzyme-linked immunosorbent assay (ELISA) kit. Different sample preparation methods were compared, and a simple extraction protocol with acidified acetonitrile/water (3:1) followed by hexane partitioning cleanup was found to be most effective. Thorough validation of the final method was conducted, and 90– 115% recoveries were achieved for all analytes except for MC-RR, which gave 130% average recovery (isotopically labeled internal standards were unavailable to correct for possible biases). The use of electrospray ionization in the negative mode gave few interferences and minimal matrix effects in the LC-MS/MS analysis overall. Precision was typically 10–20% RSD among multiple days in experiments, detection limits were <10 ng/g in the fish tissue (catfish, basa, and swai filets), and no false-positives or false-negatives occurred in blind analyses of many spiked samples. The ELISA was unable to distinguish between MCs but was found to correctly assess the presence or absence of MCs and Nod-R in the blind-fortified fish tissues. The capability of these approaches to measure covalently bound MCs was not assessed

Autor

Geis Asteggiante, Lucía
Lehotay, S. J.
Fortis, L. L.
Paoli, G.
Wijey, C.

Fuente

Analytical and Bioanalytical Chemistry v.401, no. 8, 2011. -- p. 2617-2630

Editor

Springer

Fecha

2011

Derechos

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

DOI 10.1007/s00216-011-5345-0
Fecha de agregación
August 28, 2013
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
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Citación
Geis Asteggiante, Lucía, “Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed May 28, 2020, http://riquim.fq.edu.uy/items/show/831.
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