Sterolibacterium denitrifican gen. nov. : sp. nov.

BACTERIAS

MICROBIOLOGIA

A bacterial strain (Chol-1ST) that is able to oxidize cholesterol to CO2 and reduce nitrate to dinitrogen was enriched and isolated from an upflow sludge bed (USB) anoxic reactor that treats sanitary landfill leachate from the city of Montevideo, Uruguay. Cells of strain Chol-1ST were Gram-negative, rod-shaped to slightly curved, measured 0?5–0?661?0-1?3 mm and were motile by a single polar flagellum. Strain Chol-1ST grew optimally at 30–32 °C and pH 7?0, with a doubling time of 44–46 h when cholesterol was used as the sole carbon and energy source. The metabolism of strain Chol-1ST was strictly respiratory, with oxygen or nitrate as the terminal electron acceptor. The presence of ubiquinone Q-8 as the sole respiratory lipoquinone indicated that strain Chol-1ST belonged to the b-subclass of the Proteobacteria. Phosphatidylethanolamine was the predominant polar lipid and the G+C content of the DNA was 65?3 mol%. The fatty acid profile of strain Chol-1ST, cultivated under denitrifying conditions by using a defined mineral medium supplemented with cholesterol, was characterized by the following major components: summed feature 4 (C16 : 1v7c and/or iso C15 : 0 2-OH), C16 : 0, C18 : 1v7c and hydroxy acid C10 : 0 3-OH. Minor components included C10 : 0, C11 : 0, C12 : 0, C14 : 0, C15 : 0, C19 : 0, C19 : 0 10-methyl and hydroxylated acids C8 : 0 3-OH and C16 : 0 3-OH. Analysis of the 16S rDNA sequence showed that strain Chol-1ST represents a separate lineage within the Thauera, Azoarcus, Zoogloea and Rhodocyclus assemblage of the b-Proteobacteria. Strain Chol-1ST had highest sequence similarity (96?5%) with strain 72Chol, a denitrifying b-Proteobacterium.On the basis of polyphasic evidence, strain Chol-1ST (=DSM 13999T=ATCC BAA-354T) is proposed as the type strain of Sterolibacterium denitrificans gen. nov., sp. nov

International Journal of Systematic and Evolutionary Microbiology  v. 53, , 2003. -- p. 1085-1091

IUMS

2003

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DOI 10.1099/ijs.0.02039-0