Maximizing the practical value and investigating the retention characteristics of a remodified first-generation monolith

MONOLITOS

MONOLITOS DE SILICE

BIBLIOGRAFIA NACIONAL QUIMICA

2015

This study illustrates a procedure for producing different high-performance liquid chromatography (HPLC) stationary phases, on demand, for silica monoliths. Two commercial first-generation analytical scale bare silica monoliths were subjected to a “remodification” process that involved the following: (1) coating of the silica through an in situ silylation procedure to bond ligands of choice to the surface, (2) periodic chromatographic characterization of the coated surface over 2,000 column volumes, and (3) removing the bonded ligands using a 0.1 M hydrochloric (HCl) acid wash, to regenerate the initial silica surface. This remodification protocol was repeated for three cycles to fabricate monoliths with selected functionalities. To test reproducibility, this study was conducted on two different commercial silica monoliths subjected to this treatment involving synthetic reactions with organo-silanes and subsequent stripping with acid washes. Both monoliths were functionalized with three different coatings, without significant degradation of the initial columns' silica infrastructure and excellent stability (tested up to 2,000 column volumes). One of the monoliths had measured theoretical plates per meter (N/m) of 57,000 N/m for the original silica surface, 105,000 N/m for the first cyano coating, and 60,000 N/m for the third phenyl coating. The stationary phases prepared by remodification possessed similar selectivities to those synthesized from new silica monoliths. The results of the Tanaka test shows evidence of residual ligands of previous coatings. However, the methylene and phenyl selectivity proved to display the same characteristics as other modified monoliths of the same functionality, not subjected to the remodified proces

Microchemical Journal v. 120, 2015. -- p. 18-25

Elsevier

Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derecho de autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.

PDF

Inglés

Artículo

DOI:10.1016/j.microc.2014.12.009