Genotypic and Phenotypic Characterization of Streptomyces Species Causing Potato Common Scab in Uruguay

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Título

Genotypic and Phenotypic Characterization of Streptomyces Species Causing Potato Common Scab in Uruguay

Tema

PAPAS
URUGUAY
SARNA
STREPTOMYCES
BILIOGRAFIA NACIONAL QUIMICA
2017

Abstract

Isolation and characterization of common scab (CS) pathogen Streptomyces spp. from Uruguayan potato tubers and soil samples were done in response to significant economic losses due to CS on potato in autumn 2010. Seventy of the 331 isolates were classified as pathogenic owing to their ability to induce necrosis on tuber disks and stunting of radish seedling. Streptomyces spp. causing CS on potato in Uruguay were found to represent a range of different species by virtue of their diverse morphological and physiological traits as well as rep-PCR, rpoB phylogenetic analysis, and multi-locus sequences analysis. We identified isolates primarily as Streptomyces scabiei, S. acidiscabies, and S. europaeiscabiei. However, some of the pathogenic isolates still remain to be identified at the species level. This highlights the need for improved methods for discrimination among pathogenic Streptomyces species. The presence of Streptomyces pathogenicity island (PAI) genes was analyzed, including genes encoding for thaxtomin synthetase (txtA, txtB), tomatinase (tomA), and a necrosis protein (nec1). Among the isolates that were pathogenic, 50% contained the four pathogenicity genes, 33% had an atypical composition of PAI marker genes, and 17% did not contain any genes. The absence of the genes reported to be involved in thaxtomin biosynthesis (txtA, txtB) was confirmed by whole-genome sequencing of two representative strains of this group. This finding suggests the participation of other virulence factors in plant pathogenicity.

Autor

Lapaz, M. I.
Huguet Tapia, J. C.
Verdier, E.
Loria, R.

Fuente

Plant disease v. 101, 2017.-- p. 1362-1372

Editor

The American Phytopathological Society

Fecha

2017

Derechos

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

DOI: 10.1094/PDIS-09-16-1348-RE

Cobertura

Uruguay

Document Item Type Metadata

Original Format

PDF
Fecha de agregación
March 7, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
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Citación
Lapaz, M. I., “Genotypic and Phenotypic Characterization of Streptomyces Species Causing Potato Common Scab in Uruguay,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed December 17, 2018, http://riquim.fq.edu.uy/items/show/4876.
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