The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom

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Título

The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom

Tema

ANTIVENENOS
FLAVONOIDES
ANTIVENENOS DE SERPIENTE
CISSAMPELOS PAREIRA
BIBLIOGRAFIA NACIONAL QUIMICA
2018

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: Ophidian accidents are a serious public health problem in Argentina; the Bothrops species is responsible for 97% of these accidents, and in particular, B. diporus is responsible for 80% of them. In the northeast of the country (Corrientes Provinces), Cissampelos pareira L. (Menispermaceae) is commonly used against the venom of B. diporus; its use is described in almost all ethnobotanical literature from countries where the plant grows. AIM OF THE STUDY: In this study, the in vitro and in vivo antivenom activities of C. pareira extracts were evaluated against B. diporus venom, with a particular focus on the local effects associated with envenoming. The seasonal influence on the chemical composition of the active extracts was also studied, in order determine the associated range of variability and its influence on the antivenom activity. MATERIALS AND METHODS: This research was conducted using aerial parts (leaves, flowers, tender stems) and roots of Cissampelos pareira collected from two different phytogeographic regions of Corrientes (Argentina); Paso de la Patria and Lomas de Vallejos. In addition, to perform a seasonal analysis and to evaluate the metabolic stability, material was collected at three different growth stages. In vivo and in vitro anti-snake venom activities were tested, and a bio-guided chromatographic separation was performed in order to determine the active chemicals involved. The fractions obtained were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the chemical profile of the most active constituent was analyzed by ultra high-performance liquid chromatography coupled to quadrupole/high-resolution mass spectrometry (Q-Orbitrap). (UHPLC-MS). RESULTS: The alcoholic extract was found to be the most active The bio-guided fractionation allowed selection one fraction to be analyzed by UHPLC-MS in order to identify the components responsible for the activities found; this identified five possible flavonoids. CONCLUSIONS: Our studies of the activity of C. pareira against the venom of B. diporus have confirmed that this species possesses inhibitory effects in both in vitro and in vivo models. Moreover, the present data demonstrate that certain flavonoids may mitigate some of the venom-induced local tissue damage.

Autor

Ricciardi-Verrastro, Bárbara
Torres, Ana María.
Ricciardi, Gabriela
Teibler, Pamela
Maruñak, Silvana
Barnaba, Chiara
Larcher, Roberto
Nicolini, Giorgio

Fuente

Journal of Ethnopharmacolog v. 212, 2018. -- p. 36-42

Editor

Elsevier

Fecha

2018

Derechos

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

DOI: 10.1016/j.jep.2017.09.015.

Document Item Type Metadata

Original Format

PDF
Fecha de agregación
November 6, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
Ricciardi-Verrastro, Bárbara, “The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed November 14, 2018, http://riquim.fq.edu.uy/items/show/5341.
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