Alternative RNA splicing of leukocyte tissue transglutaminase in coeliac disease

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Título

Alternative RNA splicing of leukocyte tissue transglutaminase in coeliac disease

Tema

ENFERMEDAD CELIACA
TRANSGLUTAMINASA
BIBLIOGRAFIA NACIONAL QUIMICA
2018

Abstract

Tissue transglutaminase is a ubiquitous and multifunctional protein that contributes to several processes such as apoptosis/survival, efferocytosis, inflammation and tissue repairing under physiological and pathological conditions. Several activities can be associated with well-established functional domains; in addition, four RNA alternative splice variants have been described, characterized by sequence divergences and residues deletion at the C-terminal domains. Tissue transglutaminase is recognized as the central player in the physiopathology of coeliac disease (CD) mainly through calcium-dependent enzymatic activities. It can be hypothesized that differential regulation of tissue transglutaminase splice variants expression in persons with CD contributes to pathology by altering the protein functionality. We characterized the expression pattern of RNA alternative splice variants by RT-PCR in peripheral cells from patients with CD under free gluten diet adhesion; we considered inflammatory parameters and specific antibodies as markers of the stage of disease. We found significant higher expression of both the full length and the shortest C-truncated splice variants in leucocytes from patients with CD in comparison with healthy individuals. As tissue transglutaminase expression and canonical enzymatic activity are linked to inflammation, we studied the RNA expression of inflammatory cytokines in peripheral leucocytes of persons with CD in relation with splice variants expression; interestingly, we found that recently diagnosed patients showed significant correlation between both the full length and the shortest alternative spliced variants with IL-1 expression. Our results points that regulation of alternative splicing of tissue transglutaminase could account for the complex physiopathology of CD.

Autor

Arbildi Torres, Paula
Sóñora, Cecilia.
Del Río, Natalia
Marques, Juan Martín
Hernández, Ana

Fuente

Scandinavian Journal of Immunology v.87, no. 5, 2018. --e12659

Editor

Wiley

Fecha

2018

Derechos

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

DOI: 10.1111/sji.12659

Document Item Type Metadata

Original Format

PDF
Fecha de agregación
December 4, 2018
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
,
Citación
Arbildi Torres, Paula, “Alternative RNA splicing of leukocyte tissue transglutaminase in coeliac disease,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed December 19, 2018, http://riquim.fq.edu.uy/items/show/5500.
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