Evaluation of Mycobacterium smegmatis as indicator of the efficacy of high hydrostatic pressure and ultra-high pressure homogenization treatments for pasteurization-like purposes in milk

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Título

Evaluation of Mycobacterium smegmatis as indicator of the efficacy of high hydrostatic pressure and ultra-high pressure homogenization treatments for pasteurization-like purposes in milk

Tema

LECHE
PASTEURIZACION
MYCOBACTERIUM SMEGMATIS
BIBLIOGRAFIA NACIONAL QUIMICA
2020

Abstract

The objectives of this study were: to assess the efficiency of high hydrostatic pressure or ultrahigh
pressure homogenization against Mycobacterium smegmatis in milk and to discuss whether M. smegmatis can be considered a suitable surrogate for other Mycobacterium spp.
in high pressure inactivation trials using milk. Three strains of this specie (CECT 3017, 3020 and 3032) were independently inoculated into both skimmed (0.2% fat) and whole milk (3.4% fat) at an approximate load of 6.5 Log CFU/ml and submitted to HHP treatments at 300, 400 or 500 MPa for 10m at 6°C and 20°C. Evolution of the surviving cells of the inoculated strains was evaluated analysing milk immediately after the treatments and after 5 and 8 d of storage at 6°C. HHP treatments at 300 MPa were seldom efficient at inactivating M. smegmatis strains, but lethality increased with pressure applied in all cases. Generation of sub-lethal injured cells was observed only after 400 MPa treatments since inactivation at
500 MPa was shown to be complete. Significant differences were not observed due to either
temperature of treatment or fat content of milk, except for strain CECT3032, which was shown to be the most sensitive to HHP treatments. Milk inoculated with strain CECT3017 was submitted to ultra-high pressure homogenization (UHPH) treatments at 200, 300 and 400 MPa. Maximum reductions were obtained after 300 and 400 MPa treatments, although less than 3.50 Log CFU/ml were inactivated. UHPH did not cause significant number of injured cells. The usefulness of this species as a marker for pressure-based processing
seems limited since it showed greater sensitivity than some pathogenic species including
other Mycobacteria reported in previous studies.

Autor

Velázquez-Estrada, Rita M. 
López Pedemonte, Tom
Hernández Herrero, María Manuela
Roig-Sagués, Artur Xavier

Fuente

Journal of Dairy Research 2020.-- p. 1-9

Editor

Cambridge University Press

Fecha

2020

Derechos

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Pdf

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Inglés

Tipo

Artículo

Identificador

https://doi.org/10.1017/S0022029919001043

Document Item Type Metadata

Original Format

Pdf
Fecha de agregación
September 20, 2020
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
Etiquetas
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Citación
Velázquez-Estrada, Rita M., “Evaluation of Mycobacterium smegmatis as indicator of the efficacy of high hydrostatic pressure and ultra-high pressure homogenization treatments for pasteurization-like purposes in milk,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed October 29, 2020, http://riquim.fq.edu.uy/items/show/6138.
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