Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate

Dublin Core

Título

Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate

Tema

ACEITES ESENCIALES
CITRICOS
POMELO
LIMON
BERGAMOTA
HELMINTOS
PARASITOS
BIBLIOGRAFIA NACIONAL QUIMICA
2021

Abstract

Haemonchus contortus, a blood-sucking parasite of small ruminants, produces very important economic losses in the productive sector. This abomasum parasite has become resistant to most commercial drugs worldwide, and alternatives to fight this problem are urgently needed. Essential oils (EO) are a complex mixture of volatile secondary metabolites, composed mainly by terpenoids and phenolic compounds, from plants that have several pharmacological properties, including anthelmintic activity. Particularly, citrus peel is a source of cold-pressed EO, where limonene is its major component, and can be used as an additional food component for ruminants. The
aim of the present work was to determine the in vitro anthelmintic activity of EO from Citrus bergamia (EOB), C. x
paradisii (EOG) and limonene against the benzimidazole-susceptible Kirby isolate of H. contortus, using the egg hatch test (EHT) and the exsheathed third stage larval motility test (XLMT) using a WMicroTracker equipment. Albendazole (ABZ) and monepantel (MON) were used as positive controls. The 50% inhibitory concentrations (IC50) in XLMT were 8.77 and 13.88 μg/ml for EOB and EOG respectively, after an incubation of 72 h. An interesting observation on XLMT resulted when the positive controls were tested on the same plate, but in different well of the EOB. The volatile components of the EO significantly influenced (P < 0.05) the percentage of larval motility, reducing values from 66.9 to 19.6% for ABZ, and from 72.8 to 33.7% for MON, when comparing the activity of positive controls in a control plate without EO. The in vitro anthelmintic activity of EOB and EOG shows that they could be interesting candidates for nematode control. It is still necessary additional studies
against the adult stage of H. contortus in efficacy trials in infected animals to validate their anthelmintic activity.

Autor

Garbin, Vivien Patricia
Munguía, Beatriz
Saldaña, Jenny Carolina
Deschamps, Cícero
Raupp Cipriano, Roger
Beltrao Molento, Marcelo

Fuente

Acta Tropica v. 217, 2021. -- e105869

Editor

Elsevier

Fecha

2021

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

10.1016/j.actatropica.2021.105869

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Original Format

PDF
Fecha de agregación
October 14, 2021
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
Garbin, Vivien Patricia, “Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed December 8, 2021, http://riquim.fq.edu.uy/items/show/6380.
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