Purification of protein from Arthrospira platensis using aqueous two-phase system associate with membrane separation process and evaluation of functional properties 

Arthrospira platensis has stood out, especially due to its high protein content and its potential for food application. However,
the exploration of protein concentration techniques of this microalgae still presents a great challenge. Thus, the objective
of this study was to evaluate the purification of A. platensis proteins by applying two different aqueous two-phase systems
(ATS) composed of potassium phosphate and ethanol, and sodium citrate and ethanol, in addition to the association with
membrane separation process (MSP), and the characterization of the fractions obtained. ATS under the optimised conditions
(19% potassium phosphate and 30% ethanol, and 19.5% sodium citrate and 29% ethanol) resulted in 1.27 and 1.31 purity, and 77.45% and 78% recovery, respectively. The sodium citrate and ethanol ATS showed significantly greater purity; thus, the upper phase was submitted to MSP. The association of ATS and MSP increased the recovery (~ 87%) and the purity (1.5). The top phase of ATS showed higher protein content (61%) than the bottom (5.9%), confirming the protein preference to the top. When the MSP was evaluated, the protein was increased about 8.0%, and antioxidant activity was kept (42 and 40% to top phase and ATS/MSP concentrate, respectively). Emulsions formed by ATS top phase showed a lower droplet size (33.69 μm D4.3 and 6.02 μm D3.2), and a significantly lower degree of overall destabilization (0.04 × 10–
6 s−1 kh and 3.54 × 10–3 s−1 ks) than those stabilized with bottom phase and MSP/ATS concentrate. ATS associated with membrane process showed efficiency in the concentration A. platensis protein, being able to be an alternative to be used in industrial systems.

Información sobre Derechos de Autor

(Por favor lea este aviso antes de abrir los documentos u objetos)

La legislación uruguaya protege el derechode autor sobre toda creación literaria, científica o artística, tanto en lo que tiene que ver con sus derechos morales, como en lo referente a los derechos patrimoniales con sujeción a lo establecido por el derecho común y las siguientes leyes (LEY 9.739 DE 17 DE DICIEMBRE DE 1937 SOBRE PROPIEDAD LITERARIA Y ARTISTICA CON LAS MODIFICACIONES INTRODUCIDAS POR LA LEY DE DERECHO DE AUTOR Y DERECHOS CONEXOS No. 17.616 DE 10 DE ENERO DE 2003, LEY 17.805 DE 26 DE AGOSTO DE 2004, LEY 18.046 DE 24 DE OCTUBRE DE 2006 LEY 18.046 DE 24 DE OCTUBRE DE 2006)

ADVERTENCIA: La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso: Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con fines de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes debe indicarse el nombre de la persona autora.