Phylogeny and identification of pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay

Dublin Core

Título

Phylogeny and identification of pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay

Tema

ALLIUM CEPA
CEBOLLA
ENFERMEDADES DE LAS PLANTAS
BACTERIAS
BIBLIOGRAFIA NACIONAL QUIMICA
2022

Abstract

Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens.

Autor

De Armas, Stefanie
Galván, Guillermo A.
Lapaz, María I.
González Barrios, Pablo
Vicente, Esteban

Fuente

Plant disease v. 106, n° 4 , 2022. -- pp. 1216-1225

Editor

APS

Fecha

2022

Derechos

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

10.1094/PDIS-06-21-1140-RE

Cobertura

URUGUAY

Document Item Type Metadata

Original Format

PDF
Fecha de agregación
June 6, 2022
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
De Armas, Stefanie, “Phylogeny and identification of pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed April 24, 2024, https://riquim.fq.edu.uy/items/show/6520.
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