Secretion and detection of defensive compounds by the red flour beetle tribolium castaneum interacting with the insect pathogenic fungus beauveria bassiana

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Título

Secretion and detection of defensive compounds by the red flour beetle tribolium castaneum interacting with the insect pathogenic fungus beauveria bassiana

Tema

ECOLOGIA QUIMICA
BENZOQUINONAS
EXPRESION GENICA
PROTEINAS
BIBLIOGRAFIA NACIONAL QUIMICA
2022

Abstract

Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4- benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungustreated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.

Autor

Davyt Colo, Belén
Girotti, Juan R.
González, Andrés
Pedrini, Nicolás

Fuente

Pathogens v. 11, n° 5, 2022. -- pp. 1-10. -- e487

Editor

MDPI

Fecha

2022

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Formato

PDF

Idioma

Inglés

Tipo

Artículo

Identificador

10.3390/pathogens11050487

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Original Format

PDF
Fecha de agregación
June 6, 2022
Colección
Bibliografía Nacional Química
Tipo de Elemento
Document
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Citación
Davyt Colo, Belén, “Secretion and detection of defensive compounds by the red flour beetle tribolium castaneum interacting with the insect pathogenic fungus beauveria bassiana,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed February 29, 2024, https://riquim.fq.edu.uy/items/show/6519.
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