Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia.

Dublin Core

Title

Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia.

Subject

ASPERGILLUS NIDULANS CONIDIA
MICROBIOLOGIA
BIBLIOGRAFIA NACIONAL QUIMICA
2004

Abstract

Aspergillus nidulans possesses three well-characterized purine transporters encoded by the genes uapA , uapC and azgA . Expression of these genes in mycelium is induced by purines and repressed by ammonium or glutamine through the action of the pathwayspecific UaY regulator and the general GATA factor AreA respectively. Here, we describe the regulation of expression of purine transporters during conidiospore germination and the onset of mycelium development. In resting conidiospores, mRNA steady-state levels of purine transporter genes and purine uptake activities are undetectable or very low. Both mRNA steady-state levels and purine transport activities increase substantially during the isotropic growth phase of conidial germination. Both processes occur in the absence of purine induction and independently of the nitrogen source present in the medium. The transcriptional activator UaY is dispensable for the germination-induced expression of the three transporter genes. AreA, on the other hand, is essential for the expression of uapA , but not for that of azgA or uapC , during germination. Transcriptional activation of uapA , uapC and azgA during germination is also independent of the presence of a carbon source in the medium. This work establishes the presence of a novel system triggering purine transporter transcription during germination. Similar results have been found in studies on the expression of other transporters in A. nidulans , suggesting that global expression of transporters might operate as a general system for sensing solute availability.

Creator

Amilis, S
Koukaki, M
Scazzocchio, Claudio
Diallinas, George

Source

Molecular Microbiology v. 52, no. 1, 2004. -- p. 205-216

Publisher

Blackwell

Date

2004

Rights

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Format

PDF

Language

Inglés

Type

Artículo

Identifier

doi:10.1046/j.1365-2958.2003.03956.x

Document Item Type Metadata

Original Format

PDF
Date Added
April 14, 2015
Collection
Bibliografía Nacional Química
Item Type
Document
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Citation
Amilis, S et al., “Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia.,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed November 17, 2025, https://riquim.fq.edu.uy/items/show/2736.