Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii

Dublin Core

Title

Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii

Subject

TUBERCULOS
BACTERIAS
SOLANUM COMMERSONII
BACTERIAS-RESISTENCIA
BIBLIOGRAFIA NACIONAL QUIMICA
2017

Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.

Creator

Boschi, Federico
Schvartzman, Claudia
Murchio, Sara
Ferreira, Virginia
Galván, Guillermo A.
Smoker, Matthew
Stransfeld, Lena
Zipfel, Cyrill
Vilaró, Francisco L.
Dalla Rizza, Marco

Source

Frontiers in Plant Science v.8, 2017. -- 11p.-- e1642

Publisher

Frontiers Media

Date

2017

Rights

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Format

PDF

Language

Inglés

Type

Artículo

Identifier

DOI: 10.3389/fpls.2018.00058

Document Item Type Metadata

Original Format

PDF
Date Added
January 11, 2018
Collection
Bibliografía Nacional Química
Item Type
Document
Tags
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Citation
Boschi, Federico et al., “Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed November 17, 2025, https://riquim.fq.edu.uy/items/show/4822.