Analysis of the NCR Mechanisms in Hanseniaspora vineae and Saccharomyces cerevisiae During Winemaking

Dublin Core

Title

Analysis of the NCR Mechanisms in Hanseniaspora vineae and Saccharomyces cerevisiae During Winemaking

Subject

AMINOACIDOS
NITROGENO
FERMENTACION ALCOHOLICA
LEVADURAS
VINOS
SACCHAROMYCES CEREVISIAE
HANSENIASPORA VINEAE
BIBLIOGRAFIA NACIONAL QUIMICA
2019

Abstract

There is increasing interest in the use of non-Saccharomyces yeasts in winemaking due to their positive attributes. The non-Saccharomyces yeast Hanseniaspora vineae is an apiculate yeast that has been associated with the production of wine with good fermentation capacity and an increase in aromatic properties. However, this yeast represents a concern in mixed culture fermentation because of its nutrient consumption, especially nitrogen, as its mechanisms of regulation and consumption are still unknown. In this study, we analyzed the nitrogen consumption, as well as the nitrogen catabolism repression (NCR) mechanism, in two genome-sequenced H. vineae strains, using synthetic must fermentations. The use of synthetic must with an established nitrogen content allowed us to study the NCR mechanism in H. vineae, following the amino acid and ammonia consumption, and the expression of genes known to be regulated by the NCR mechanism in S. cerevisiae, AGP1, GAP1, MEP2, and PUT2. H. vineae exhibited a similar amino acid consumption and gene expression profile to S. cerevisiae. However, the wine strain of S. cerevisiae QA23 consumed ammonia and valine more quickly and, in contrast, tyrosine and tryptophan more slowly, than the H. vineae strains. Our results showed a similar behavior of nitrogen regulation in H. vineae and S. cerevisiae, indicating the presence of the NCR mechanism in this Hanseniaspora yeast differentiated before the whole genome duplication event of the Saccharomyces complex. Future study will elucidate if the NCR mechanism is the only strategy used by H. vineae to optimize nitrogen consumpti

Creator

Lleixá, Jessica
Martín, Valentina
Giorello, Facundo.
Portillo, María C.
Beltran, Gemma
Mas, Albert

Source

Frontiers in Genetics v. 9, 2019.-- 9p.--e747

Publisher

Frontiers Media S.A.

Date

2019

Rights

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Format

PDF

Language

Inglés

Type

Artículo

Identifier

DOI: 10.3389/fgene.2018.00747

Document Item Type Metadata

Original Format

PDF
Date Added
July 26, 2019
Collection
Bibliografía Nacional Química
Item Type
Document
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Citation
Lleixá, Jessica et al., “Analysis of the NCR Mechanisms in Hanseniaspora vineae and Saccharomyces cerevisiae During Winemaking,” RIQUIM - Repositorio Institucional de la Facultad de Química - UdelaR, accessed November 17, 2025, https://riquim.fq.edu.uy/items/show/5809.